The increasing relevance and importance of zoning regional biotic factors and environmental variables to scientists and management agencies are fully introduced. Comprehensive research into regional differentiation of...The increasing relevance and importance of zoning regional biotic factors and environmental variables to scientists and management agencies are fully introduced. Comprehensive research into regional differentiation of ecosystems, nevertheless, remains comparatively neglected. A rounded eco-regionalization System in terms of regional ecosystem properties and distribution can supply powerful tools for rational and sustainable utilization of natural resources, solution of regional environmental problems, and conservation of biodiversity. In this paper, ideas on nature, theoretical foundation and methods available to eco-regionalization are presented Finally, a new ecoregionalization system of China based on specific principles of ecological division is prepared.展开更多
The present study induced in vitro-cultured passage 4 bone marrow-derived mesenchymal stem cells to differentiate into neural-like cells with a mixture of alkaloid, polysaccharide, aglycone, glycoside, essential oils,...The present study induced in vitro-cultured passage 4 bone marrow-derived mesenchymal stem cells to differentiate into neural-like cells with a mixture of alkaloid, polysaccharide, aglycone, glycoside, essential oils, and effective components of Buyang Huanwu decoction （active principle region of decoction for invigorating yang for recuperation）. After 28 days, nestin and neuron-specific enolase were expressed in the cytoplasm. Reverse transcription-PCR and western blot analyses showed that nestin and neuron-specific enolase mRNA and protein expression was greater in the active principle region group compared with the original formula group. Results demonstrated that the active principle region of Buyang Huanwu decoction induced greater differentiation of rat bone marrow-derived mesenchymal stem cells into neural-like cells in vitro than the original Buyang Huanwu decoction formula.展开更多
目的分析不同剂量养心通脉有效部位方(apr-YTF)对心肌缺血再灌注损伤(MIRI)模型大鼠心肌线粒体代谢相关酶及其转运的影响。方法本实验时间为2020年6月至2021年6月。将70只健康雄性Wistar大鼠随机分为空白对照组、模型组、阴性对照组、...目的分析不同剂量养心通脉有效部位方(apr-YTF)对心肌缺血再灌注损伤(MIRI)模型大鼠心肌线粒体代谢相关酶及其转运的影响。方法本实验时间为2020年6月至2021年6月。将70只健康雄性Wistar大鼠随机分为空白对照组、模型组、阴性对照组、阳性对照组及apr-YTF低、中、高剂量组,每组10只。除空白对照组外,其他组大鼠构建MIRI模型,造模期间,各组大鼠共死亡25只,为保证实验完整性,补充造模至每组10只。造模完成后,空白对照组及模型组大鼠正常饲养,阴性对照组大鼠给予0.9%氯化钠溶液灌胃,阳性对照组大鼠给予冠心苏合丸灌胃,apr-YTF低、中、高剂量组大鼠分别给予1.5、3.0、6.0 ml apr-YTF灌胃,1次/d,共干预2周。比较空白对照组和模型组造模24 h后血液流变学指标(包括全血低切、中切、高切黏度)、血清心肌梗死标志物[肌酸激酶同工酶(CKMB)、心肌肌钙蛋白I(cTnI)、乳酸脱氢酶(LDH)]水平。比较七组大鼠造模第14天心肌组织病理学检查结果、线粒体代谢相关酶[ATP、磷酸果糖激酶1(PFK1)、琥珀酸脱氢酶(SDH)、腺苷酸转运体(ANT)、异枸橼酸脱氢酶(IDH)]水平及心肌组织中肿瘤坏死因子α诱导蛋白2(TNFαIP2)、MIRO1表达水平。结果模型组全血低切、中切黏度及血清CK-MB、cTnI、LDH水平高于空白对照组(P<0.05)。模型组ATP水平低于空白对照组,模型组、阴性对照组PFK1、SDH、ANT、IDH水平高于空白对照组(P<0.05);阴性对照组、阳性对照组及apr-YTF低、中、高剂量组ATP水平高于模型组,阳性对照组及apr-YTF中、高剂量组PFK1、ANT、IDH水平低于模型组,阳性对照组及apr-YTF低、中、高剂量组SDH水平低于模型组(P<0.05);apr-YTF低剂量组ATP、ANT水平高于阳性对照组,apr-YTF低、中剂量组PFK1、IDH水平高于阳性对照组(P<0.05);apr-YTF中剂量组ATP水平低于apr-YTF低剂量组,apr-YTF中、高剂量组PFK1、ANT、IDH水平低于apr-YTF低剂�展开更多
文摘The increasing relevance and importance of zoning regional biotic factors and environmental variables to scientists and management agencies are fully introduced. Comprehensive research into regional differentiation of ecosystems, nevertheless, remains comparatively neglected. A rounded eco-regionalization System in terms of regional ecosystem properties and distribution can supply powerful tools for rational and sustainable utilization of natural resources, solution of regional environmental problems, and conservation of biodiversity. In this paper, ideas on nature, theoretical foundation and methods available to eco-regionalization are presented Finally, a new ecoregionalization system of China based on specific principles of ecological division is prepared.
基金supported by the Natural Science Foundation of Anhui Province (Role of substance P in electroacupuncture for focal brain ischemia in rats), No. 050431003the National Natural Science Foundation of China (Target point and signal transduction pathway of Yangxin Tongmai active principle region for BMSCs), No. 81102595
文摘The present study induced in vitro-cultured passage 4 bone marrow-derived mesenchymal stem cells to differentiate into neural-like cells with a mixture of alkaloid, polysaccharide, aglycone, glycoside, essential oils, and effective components of Buyang Huanwu decoction （active principle region of decoction for invigorating yang for recuperation）. After 28 days, nestin and neuron-specific enolase were expressed in the cytoplasm. Reverse transcription-PCR and western blot analyses showed that nestin and neuron-specific enolase mRNA and protein expression was greater in the active principle region group compared with the original formula group. Results demonstrated that the active principle region of Buyang Huanwu decoction induced greater differentiation of rat bone marrow-derived mesenchymal stem cells into neural-like cells in vitro than the original Buyang Huanwu decoction formula.
文摘目的分析不同剂量养心通脉有效部位方(apr-YTF)对心肌缺血再灌注损伤(MIRI)模型大鼠心肌线粒体代谢相关酶及其转运的影响。方法本实验时间为2020年6月至2021年6月。将70只健康雄性Wistar大鼠随机分为空白对照组、模型组、阴性对照组、阳性对照组及apr-YTF低、中、高剂量组,每组10只。除空白对照组外,其他组大鼠构建MIRI模型,造模期间,各组大鼠共死亡25只,为保证实验完整性,补充造模至每组10只。造模完成后,空白对照组及模型组大鼠正常饲养,阴性对照组大鼠给予0.9%氯化钠溶液灌胃,阳性对照组大鼠给予冠心苏合丸灌胃,apr-YTF低、中、高剂量组大鼠分别给予1.5、3.0、6.0 ml apr-YTF灌胃,1次/d,共干预2周。比较空白对照组和模型组造模24 h后血液流变学指标(包括全血低切、中切、高切黏度)、血清心肌梗死标志物[肌酸激酶同工酶(CKMB)、心肌肌钙蛋白I(cTnI)、乳酸脱氢酶(LDH)]水平。比较七组大鼠造模第14天心肌组织病理学检查结果、线粒体代谢相关酶[ATP、磷酸果糖激酶1(PFK1)、琥珀酸脱氢酶(SDH)、腺苷酸转运体(ANT)、异枸橼酸脱氢酶(IDH)]水平及心肌组织中肿瘤坏死因子α诱导蛋白2(TNFαIP2)、MIRO1表达水平。结果模型组全血低切、中切黏度及血清CK-MB、cTnI、LDH水平高于空白对照组(P<0.05)。模型组ATP水平低于空白对照组,模型组、阴性对照组PFK1、SDH、ANT、IDH水平高于空白对照组(P<0.05);阴性对照组、阳性对照组及apr-YTF低、中、高剂量组ATP水平高于模型组,阳性对照组及apr-YTF中、高剂量组PFK1、ANT、IDH水平低于模型组,阳性对照组及apr-YTF低、中、高剂量组SDH水平低于模型组(P<0.05);apr-YTF低剂量组ATP、ANT水平高于阳性对照组,apr-YTF低、中剂量组PFK1、IDH水平高于阳性对照组(P<0.05);apr-YTF中剂量组ATP水平低于apr-YTF低剂量组,apr-YTF中、高剂量组PFK1、ANT、IDH水平低于apr-YTF低剂�
