Objective With the improvement of people＇s living standards, people＇s requests for beauty are increasing. Skin whitening and lightening have become the pursuit of many women, and whitening and removing freckles have...Objective With the improvement of people＇s living standards, people＇s requests for beauty are increasing. Skin whitening and lightening have become the pursuit of many women, and whitening and removing freckles have become the focus of scientific research. At present, widely used whitening agents, such as kojic acid, vitamin C, and its derivatives, have shortcomings such as poor stability and retarded effect. Therefore, safer and more effective whitening products from herbs are urgently needed. To explore the possibility of triterpenes as whitening active substance, the effects of total triterpenes of Poria（TTP） and poricoic acid A（PAA） on mushroom tyrosinase activities and B16 cells were investigated, and their mechanisms on mushroom tyrosinase were also studied. Methods Using arbutin and nicotinamide as reference substances, we determinated the inhibitory effects of TTP and PAA on mushroom tyrosinase and tyrosinase in B16 cells and then studied the inhibitory mechanism on mushroom tyrosinase. Results TTP and PAA exhibited good inhibitory effects on the activities of monophenolase and diphenolase in mushroom tyrosinase, as well as a certain inhibitory effect on tyrosinase in B16 cells. Conclusion TTP and PAA are potential whitening active ingredients.展开更多
文摘[目的]建立同时检测新麦纤散中5种成分:三七皂苷R1、人参皂苷Rb1、Coelonin、薯蓣皂苷和茯苓酸含量的方法。[方法]采用反相高效液相色谱(reversed phase-high performance liquid chromatography,RP-HPLC)梯度洗脱法测定三七皂苷R1、人参皂苷Rb1、Coelonin、薯蓣皂苷、茯苓酸的含量。色谱条件:phenomenex-C18色谱柱(250mm×4.6mm,5μm),流动相乙腈和0.1%磷酸水梯度洗脱,流速1.0 m L·min^(-1),柱温30℃,检测波长为203nm。[结果]三七皂苷R1、人参皂苷Rb1、Coelonin、薯蓣皂苷、茯苓酸分别在0.65~16.25μg·mL^(-1)(r^2=0.9996),0.42~10.50μg·mL^(-1)(r^2=0.9997),0.08~2.00μg·mL^(-1)(r^2=0.9998),0.37~9.25μg·mL^(-1)(r^2=0.9986),0.16~4.00μg·mL^(-1)(r^2=0.9999)范围内线性关系良好,平均加样回收率分别为98.53%、99.02%、98.55%、95.43%、97.66%,相对标准偏差(relative standard deviation,RSD)分别为1.27%、0.54%、0.53%、1.48%、0.81%。[结论]RP-HPLC梯度洗脱法操作简便,结果准确,精密度高,重现性好,能同时测定新麦纤散中三七皂苷R1、人参皂苷Rb1、Coelonin、薯蓣皂苷、茯苓酸的含量,可为完善新麦纤散的质量控制方法提供科学的依据。
基金Project of National Twelve-Five Year Research Program of China(2011BAI06B03-2 and 2012BAI29B03)
文摘Objective With the improvement of people＇s living standards, people＇s requests for beauty are increasing. Skin whitening and lightening have become the pursuit of many women, and whitening and removing freckles have become the focus of scientific research. At present, widely used whitening agents, such as kojic acid, vitamin C, and its derivatives, have shortcomings such as poor stability and retarded effect. Therefore, safer and more effective whitening products from herbs are urgently needed. To explore the possibility of triterpenes as whitening active substance, the effects of total triterpenes of Poria（TTP） and poricoic acid A（PAA） on mushroom tyrosinase activities and B16 cells were investigated, and their mechanisms on mushroom tyrosinase were also studied. Methods Using arbutin and nicotinamide as reference substances, we determinated the inhibitory effects of TTP and PAA on mushroom tyrosinase and tyrosinase in B16 cells and then studied the inhibitory mechanism on mushroom tyrosinase. Results TTP and PAA exhibited good inhibitory effects on the activities of monophenolase and diphenolase in mushroom tyrosinase, as well as a certain inhibitory effect on tyrosinase in B16 cells. Conclusion TTP and PAA are potential whitening active ingredients.
