Two new xanthone glycosides, polygalaxanthone IV and V were isolated from the roots of Polygala tenuifolia Willd. Their structures were established as 6-O-[a-L-rhamnopyranosyl- (12)-b-D-glucopyranosyl]-1-hydroxy-3, 7...Two new xanthone glycosides, polygalaxanthone IV and V were isolated from the roots of Polygala tenuifolia Willd. Their structures were established as 6-O-[a-L-rhamnopyranosyl- (12)-b-D-glucopyranosyl]-1-hydroxy-3, 7-dimethoxyxanthone (polygalaxanthone IV), and 6-O- [a-L-rhamnopyranosyl-(12)-b-D-glucopyranosyl]-1, 3-dihydroxy-7-methoxyxanthone (polyga- laxanthone V), respectively, on the basis of chemical and spectral evidence.展开更多
目的：建立一种远志中远志酮Ⅲ和3,6＇-二芥子酰基蔗糖含量的HPLC-MS/MS分析方法。方法：采用ZORBAX Eclipse plus C18色谱柱,（4.6 mm×100 mm,3.5μm）,流动相乙腈-0.1%甲酸水（28∶72）,流速0.3 m L·min^-1,进样量10μL,...目的：建立一种远志中远志酮Ⅲ和3,6＇-二芥子酰基蔗糖含量的HPLC-MS/MS分析方法。方法：采用ZORBAX Eclipse plus C18色谱柱,（4.6 mm×100 mm,3.5μm）,流动相乙腈-0.1%甲酸水（28∶72）,流速0.3 m L·min^-1,进样量10μL,柱温20℃。质谱条件：电喷雾负离子化模式,多反应监测模式（MRM）,检测离子对分别为m/z 567.2~345.1（远志酮Ⅲ）,m/z 753.3~205.1（3,6＇-二芥子酰基蔗糖）。结果：两种成分的线性关系良好,分析过程只需6 min。远志酮Ⅲ的日内精密度和日间精密度的RSD分别为2.2%,2.5%,回收率为96.1%~101.7%（RSD 2.4%）;3,6＇-二芥子酰基蔗糖的日内精密度和日间精密度的RSD分别为2.3%,2.4%,回收率为95.7%~101.4%（RSD 2.0%）。结论：该方法快速、灵敏、选择性好,适合远志中远志酮Ⅲ和3,6＇-二芥子酰基蔗糖的定量分析。展开更多
文摘Two new xanthone glycosides, polygalaxanthone IV and V were isolated from the roots of Polygala tenuifolia Willd. Their structures were established as 6-O-[a-L-rhamnopyranosyl- (12)-b-D-glucopyranosyl]-1-hydroxy-3, 7-dimethoxyxanthone (polygalaxanthone IV), and 6-O- [a-L-rhamnopyranosyl-(12)-b-D-glucopyranosyl]-1, 3-dihydroxy-7-methoxyxanthone (polyga- laxanthone V), respectively, on the basis of chemical and spectral evidence.
