A novel electrogenerated chemiluminescence （ECL） aptasensor for highly sensitive detection of thrombin was developed on the basis of poly（pyrrole-co-pyrrole propylic acid） nanoparticles loaded with aptamer and rut...A novel electrogenerated chemiluminescence （ECL） aptasensor for highly sensitive detection of thrombin was developed on the basis of poly（pyrrole-co-pyrrole propylic acid） nanoparticles loaded with aptamer and ruthenium complex. Thrombin binding aptamers served as the molecular recognition elements and ruthenium bis（2,2＇-bipyridine） （2,2＇-bipyridine-4,4＇-dicarboxylic acid）-ethylenediamine （Rul） was used as an ECL signal complex. Novel electroactive polymers poly（pyrrole-co-pyr- role propylic acid） nanoparticles （Ppy-pa NPs） were synthesized by a simple alcohol-assisted microemulsion polymerization. Rul-Ppy-pa NPs were synthesized by covalently coupling Rul with the Ppy-pa NPs. Ppy-pa NPs and Rul-Ppy-pa NPs were characterized using a fourier transform infrared spectrometer, super-conducting fourier digital NMR spectrometer, and trans- mission electron microscope. One ECL chemical sensor fabricated by immobilizing the Rul-Ppy-pa NPs on PIGE was developed for the determination of TprA with a high sensitivity and stability. The ECL aptasensor was fabricated by covalenfly coupling the thrombin binding aptamer-I （TBA-I） onto the surface of the paraffin-impregnated graphite electrode, which had been covalently modified with a monolayer of 4-aminobenzene sulfonic acid via electrochemical oxidations, for capturing thrombin onto the electrode and then the TBA-II labeled with Rul-Ppy-pa NPs was bound with epitope of thrombin. The ECL aptasensot showed an extremely low detection limit of 3.0×10^-16 mol/L for thrombin and a good selectivity. This work demonstrated that using Ppy-pa NPs as a carrier of ruthenium complex and molecular recognition element was a promising approach for the fabrication of ECL biosensor with high sensitivity.展开更多
基金supported by the National Natural Science Foundation of China (21027007, 20975065 & 20775046)
文摘A novel electrogenerated chemiluminescence （ECL） aptasensor for highly sensitive detection of thrombin was developed on the basis of poly（pyrrole-co-pyrrole propylic acid） nanoparticles loaded with aptamer and ruthenium complex. Thrombin binding aptamers served as the molecular recognition elements and ruthenium bis（2,2＇-bipyridine） （2,2＇-bipyridine-4,4＇-dicarboxylic acid）-ethylenediamine （Rul） was used as an ECL signal complex. Novel electroactive polymers poly（pyrrole-co-pyr- role propylic acid） nanoparticles （Ppy-pa NPs） were synthesized by a simple alcohol-assisted microemulsion polymerization. Rul-Ppy-pa NPs were synthesized by covalently coupling Rul with the Ppy-pa NPs. Ppy-pa NPs and Rul-Ppy-pa NPs were characterized using a fourier transform infrared spectrometer, super-conducting fourier digital NMR spectrometer, and trans- mission electron microscope. One ECL chemical sensor fabricated by immobilizing the Rul-Ppy-pa NPs on PIGE was developed for the determination of TprA with a high sensitivity and stability. The ECL aptasensor was fabricated by covalenfly coupling the thrombin binding aptamer-I （TBA-I） onto the surface of the paraffin-impregnated graphite electrode, which had been covalently modified with a monolayer of 4-aminobenzene sulfonic acid via electrochemical oxidations, for capturing thrombin onto the electrode and then the TBA-II labeled with Rul-Ppy-pa NPs was bound with epitope of thrombin. The ECL aptasensot showed an extremely low detection limit of 3.0×10^-16 mol/L for thrombin and a good selectivity. This work demonstrated that using Ppy-pa NPs as a carrier of ruthenium complex and molecular recognition element was a promising approach for the fabrication of ECL biosensor with high sensitivity.
