目的为制备出满足骨缺损修复需要的具有一定力学强度和生物活性的骨组织工程支架,本文选取聚己内酯(polycaprolactone,PCL)和纳米氧化锆(ZrO2)粉末制备出三维多孔复合材料支架。方法采用高温熔融挤出3D打印方式制备PCL/ZrO2复合材料支架...目的为制备出满足骨缺损修复需要的具有一定力学强度和生物活性的骨组织工程支架,本文选取聚己内酯(polycaprolactone,PCL)和纳米氧化锆(ZrO2)粉末制备出三维多孔复合材料支架。方法采用高温熔融挤出3D打印方式制备PCL/ZrO2复合材料支架,为获取支架的几何形态、力学性能和生物学性能,利用扫描电子显微镜(scanning electron microscope,SEM)和万能试验机(material test system,MTS)分别分析了支架的形貌和压缩性能,并通过体外细胞培养的方式测试复合材料支架的生物相容性。结果制备完成的复合材料支架具有良好的三维孔隙结构,孔径≥400μm,孔隙率≥40%。对比纯PCL支架,PCL/ZrO2复合材料支架的力学性能显著提高,杨氏模量提高0.4倍左右,抗压强度提高0.5倍左右。在体外实验中,细胞培养7 d后PCL/ZrO2复合材料支架上的细胞增殖对比纯PCL支架有显著提高。结论基于该结果,本文制备出的PCL/ZrO2生物活性骨组织支架在骨组织工程方面有一定的应用前景。展开更多
The distinct structural properties and osteogenic capacity are important aspects to be taken into account when developing guided bone regeneration membranes.Herein,inspired by the structure and function of natural per...The distinct structural properties and osteogenic capacity are important aspects to be taken into account when developing guided bone regeneration membranes.Herein,inspired by the structure and function of natural periosteum,we designed and fabricated using electrospinning a fibrous membrane comprising(poly)-e-caprolactone(PCL),collagen-I(Col)and mineralized Col(MC).The three-layer membranes,having PCL as the outer layer,PCL/Col as the middle layer and PCL/Col/MC in different ratios(5/2.5/2.5(PCM-1);3.3/3.3/3.3(PCM-2);4/4/4(PCM-3)(%,w/w/w))as the inner layer,were produced.The physiochemical properties of the different layers were investigated and a good integration between the layers was observed.The three-layeredmembranes showed tensile properties in the range of those of natural periosteum.Moreover,the membranes exhibited excellent water absorption capability without changes of the thickness.In vitro experiments showed that the inner layer of the membranes supported attachment,proliferation,ingrowth and osteogenic differentiation of human bone marrowderived stromal cells.In particular cells cultured on PCM-2 exhibited a significantly higher expression of osteogenesis-related proteins.The three-layered membranes successfully supported new bone formation inside a critical-size cranial defect in rats,with PCM-3 being the most efficient.The membranes developed here are promising candidates for guided bone regeneration applications.展开更多
It remains a challenge to achieve satisfactory balance between biodegradability and osteogenic capacity in biosynthetic bone grafts.In this study,we aimed to address this challenge by incorporating mesoporous bioactiv...It remains a challenge to achieve satisfactory balance between biodegradability and osteogenic capacity in biosynthetic bone grafts.In this study,we aimed to address this challenge by incorporating mesoporous bioactive glass(MBG)into poly(caprolactone-co-glycolide)(PGA-PCL)at gradient ratios.MBG/PGA-PCL(PGC/M)scaffolds with MBG incorporation ratio at 0,10%,25%and 40%(PGC/M0-40)were synthesized using a modified solvent casting-particulate leaching method,and their physiochemical and biological properties were comprehensively evaluated.PGC/M scaffolds exhibited highly perforated porous structure with a large-pore size of 300-450μm,with ordered MBGs of around 6.0 nm mesopores size uniformly dispersed.The increase in MBG incorporation ratio significantly improved the scaffold surface hydrophilicity,apatite-formation ability and pH stability,increased the weight loss rate while insignificantly influenced the molecular chains degradation of PGA-PCL component,and facilitated the attachment,spreading,viability and proliferation of rat bone marrow stromal cells(rBMSCs)on scaffolds.Moreover,rBMSCs cultured on PGC/M10-40 scaffolds demonstrated enhanced ALP activity and osteogenesis-related gene expression in a MBG dose-dependent manner as compared with those cultured on PGC/M0 scaffolds.When implanted to the rat cranial bone defect,PGC/M25 and PGC/M40 scaffolds induced significantly better bone repair as compared to PGC/M0 and PGC/M10 scaffolds.Besides,the biodegradability of PGC/M scaffolds correlated with the MBG incorporation ratio.These data suggested this novel PGC/M scaffolds as promising bone repair biomaterial with highly tunable hydrophilicity,bioactivity,cytocompatibility,osteogenic activity as well as biodegradability.展开更多
文摘目的为制备出满足骨缺损修复需要的具有一定力学强度和生物活性的骨组织工程支架,本文选取聚己内酯(polycaprolactone,PCL)和纳米氧化锆(ZrO2)粉末制备出三维多孔复合材料支架。方法采用高温熔融挤出3D打印方式制备PCL/ZrO2复合材料支架,为获取支架的几何形态、力学性能和生物学性能,利用扫描电子显微镜(scanning electron microscope,SEM)和万能试验机(material test system,MTS)分别分析了支架的形貌和压缩性能,并通过体外细胞培养的方式测试复合材料支架的生物相容性。结果制备完成的复合材料支架具有良好的三维孔隙结构,孔径≥400μm,孔隙率≥40%。对比纯PCL支架,PCL/ZrO2复合材料支架的力学性能显著提高,杨氏模量提高0.4倍左右,抗压强度提高0.5倍左右。在体外实验中,细胞培养7 d后PCL/ZrO2复合材料支架上的细胞增殖对比纯PCL支架有显著提高。结论基于该结果,本文制备出的PCL/ZrO2生物活性骨组织支架在骨组织工程方面有一定的应用前景。
基金supported by National Key R&D Program of China(2017YFC1105000)National Natural Science Foundation of China(51572087)+3 种基金Outstanding Scholar Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory(2018GZR110102001)GDSTNWO Science Industry Cooperation Program Chemistry(2018A50501006)the 111 Project(B13039).P.H.and Y.Z.acknowledge the financial support by the Gravitation Program‘Materials Driven Regeneration’,funded by the Netherlands Organization for Scientific Research(NWO)(Grant#024.003.013)J.L.and P.H.acknowledge financial support by the NWO,Applied and Engineering Sciences(NWO-AES,Grant#16711).
文摘The distinct structural properties and osteogenic capacity are important aspects to be taken into account when developing guided bone regeneration membranes.Herein,inspired by the structure and function of natural periosteum,we designed and fabricated using electrospinning a fibrous membrane comprising(poly)-e-caprolactone(PCL),collagen-I(Col)and mineralized Col(MC).The three-layer membranes,having PCL as the outer layer,PCL/Col as the middle layer and PCL/Col/MC in different ratios(5/2.5/2.5(PCM-1);3.3/3.3/3.3(PCM-2);4/4/4(PCM-3)(%,w/w/w))as the inner layer,were produced.The physiochemical properties of the different layers were investigated and a good integration between the layers was observed.The three-layeredmembranes showed tensile properties in the range of those of natural periosteum.Moreover,the membranes exhibited excellent water absorption capability without changes of the thickness.In vitro experiments showed that the inner layer of the membranes supported attachment,proliferation,ingrowth and osteogenic differentiation of human bone marrowderived stromal cells.In particular cells cultured on PCM-2 exhibited a significantly higher expression of osteogenesis-related proteins.The three-layered membranes successfully supported new bone formation inside a critical-size cranial defect in rats,with PCM-3 being the most efficient.The membranes developed here are promising candidates for guided bone regeneration applications.
基金supported by National Key R&D Program of China(2018YFC1105703)Key R&D Program of Sichuan Province(2017SZ0047).
文摘It remains a challenge to achieve satisfactory balance between biodegradability and osteogenic capacity in biosynthetic bone grafts.In this study,we aimed to address this challenge by incorporating mesoporous bioactive glass(MBG)into poly(caprolactone-co-glycolide)(PGA-PCL)at gradient ratios.MBG/PGA-PCL(PGC/M)scaffolds with MBG incorporation ratio at 0,10%,25%and 40%(PGC/M0-40)were synthesized using a modified solvent casting-particulate leaching method,and their physiochemical and biological properties were comprehensively evaluated.PGC/M scaffolds exhibited highly perforated porous structure with a large-pore size of 300-450μm,with ordered MBGs of around 6.0 nm mesopores size uniformly dispersed.The increase in MBG incorporation ratio significantly improved the scaffold surface hydrophilicity,apatite-formation ability and pH stability,increased the weight loss rate while insignificantly influenced the molecular chains degradation of PGA-PCL component,and facilitated the attachment,spreading,viability and proliferation of rat bone marrow stromal cells(rBMSCs)on scaffolds.Moreover,rBMSCs cultured on PGC/M10-40 scaffolds demonstrated enhanced ALP activity and osteogenesis-related gene expression in a MBG dose-dependent manner as compared with those cultured on PGC/M0 scaffolds.When implanted to the rat cranial bone defect,PGC/M25 and PGC/M40 scaffolds induced significantly better bone repair as compared to PGC/M0 and PGC/M10 scaffolds.Besides,the biodegradability of PGC/M scaffolds correlated with the MBG incorporation ratio.These data suggested this novel PGC/M scaffolds as promising bone repair biomaterial with highly tunable hydrophilicity,bioactivity,cytocompatibility,osteogenic activity as well as biodegradability.
