Forty-five rhizofungal isolates were isolated, identified and characterized from 11 herbicides polluted-soil. Among the isolates, 10 fungal species proved to be the most potent and promising ones in herbicides toleran...Forty-five rhizofungal isolates were isolated, identified and characterized from 11 herbicides polluted-soil. Among the isolates, 10 fungal species proved to be the most potent and promising ones in herbicides tolerance. The herbicides exhibited severe and dramatic effect and modulation on fungal DNA and protein represented in DNA and protein profile. Severely loss in the total soluble cell ions （SCI） and total cell protein percentage （TCPC） concentrations were observed. The loss of SCI by glyphosate, Aspergillus flavus （86,30%） was the most affected one, followed by Penicillium spiculisporus （76,30%）, Penicilliurn verruculosum （64.40%） and Alternaria tenuissima （64%）, respectively. For pendimethalin, Alternaria tenuissima （54.01%） was the most affected fungi. For diclofop-methyl, Penicillium spiculisporus （74.20%） was the most affected fungi. The loss of TCPC by glyphosate, Alternaria tenuissima （64.71%） was the most effected fungi, followed by Penicillium spiculisporus （57.14%）, respectively. For pendimethalin, A. terreus （54.29%） w, as the most affected fungi. For diclofop-methyl, Penicillium spiculisporus （60%） was the most affected fungi, fbllowed by Alternaria tenuissima （58.82%）, Aspergillus tamarii （55.56%）, respectively. The results proved severe reductions and alteration in protein, SCI, TCPC and DNA in fungal strains exposed to these herbicides which might reflex a degree of tolerance occurred during the assimilation of those toxic compounds from the pesticides polluted-soil.展开更多
通过对东北地区污染农田黑土剖面的取样调查分析,对重金属Cd、Pb、Cu、Zn在农田黑土中的污染状况及其迁移特征进行了对比研究,并就BCR(the Comm un ity Bureau of R eference)连续提取法在农田土壤重金属污染评估中的应用进行了探讨。...通过对东北地区污染农田黑土剖面的取样调查分析,对重金属Cd、Pb、Cu、Zn在农田黑土中的污染状况及其迁移特征进行了对比研究,并就BCR(the Comm un ity Bureau of R eference)连续提取法在农田土壤重金属污染评估中的应用进行了探讨。结果表明,重金属的迁移率大小依次为:Cd>Cu>Pb>Zn,Cd的酸可溶态占全量的比率高于其它重金属。Pb的酸可提取态浓度随土壤深度增加而增大,公路附近土壤中Pb、Cu及城市郊区土壤中Cd、Pb和Zn的氧化物结合态含量均随土壤深度的增加而升高,已存在向下迁移的趋势。4种重金属有机结合态在采样区土壤中基本都表现为耕层高于非耕层。研究表明,应用BCR连续提取法有助于确定土壤重金属污染以及重金属向下迁移状况,借助该法可以有效、可靠地对土壤重金属污染进行评估。展开更多
文摘Forty-five rhizofungal isolates were isolated, identified and characterized from 11 herbicides polluted-soil. Among the isolates, 10 fungal species proved to be the most potent and promising ones in herbicides tolerance. The herbicides exhibited severe and dramatic effect and modulation on fungal DNA and protein represented in DNA and protein profile. Severely loss in the total soluble cell ions （SCI） and total cell protein percentage （TCPC） concentrations were observed. The loss of SCI by glyphosate, Aspergillus flavus （86,30%） was the most affected one, followed by Penicillium spiculisporus （76,30%）, Penicilliurn verruculosum （64.40%） and Alternaria tenuissima （64%）, respectively. For pendimethalin, Alternaria tenuissima （54.01%） was the most affected fungi. For diclofop-methyl, Penicillium spiculisporus （74.20%） was the most affected fungi. The loss of TCPC by glyphosate, Alternaria tenuissima （64.71%） was the most effected fungi, followed by Penicillium spiculisporus （57.14%）, respectively. For pendimethalin, A. terreus （54.29%） w, as the most affected fungi. For diclofop-methyl, Penicillium spiculisporus （60%） was the most affected fungi, fbllowed by Alternaria tenuissima （58.82%）, Aspergillus tamarii （55.56%）, respectively. The results proved severe reductions and alteration in protein, SCI, TCPC and DNA in fungal strains exposed to these herbicides which might reflex a degree of tolerance occurred during the assimilation of those toxic compounds from the pesticides polluted-soil.
文摘通过对东北地区污染农田黑土剖面的取样调查分析,对重金属Cd、Pb、Cu、Zn在农田黑土中的污染状况及其迁移特征进行了对比研究,并就BCR(the Comm un ity Bureau of R eference)连续提取法在农田土壤重金属污染评估中的应用进行了探讨。结果表明,重金属的迁移率大小依次为:Cd>Cu>Pb>Zn,Cd的酸可溶态占全量的比率高于其它重金属。Pb的酸可提取态浓度随土壤深度增加而增大,公路附近土壤中Pb、Cu及城市郊区土壤中Cd、Pb和Zn的氧化物结合态含量均随土壤深度的增加而升高,已存在向下迁移的趋势。4种重金属有机结合态在采样区土壤中基本都表现为耕层高于非耕层。研究表明,应用BCR连续提取法有助于确定土壤重金属污染以及重金属向下迁移状况,借助该法可以有效、可靠地对土壤重金属污染进行评估。
