Apical points of young seedlings of wheat (Triticum aestivum) cultivar “Jing 411” and somatic calli of cultivar “FK8” were transformed with plasmids pBI121 and (or) pBIAH A + by using microprojectile bombardment. ...Apical points of young seedlings of wheat (Triticum aestivum) cultivar “Jing 411” and somatic calli of cultivar “FK8” were transformed with plasmids pBI121 and (or) pBIAH A + by using microprojectile bombardment. Histochemical assay of GUS activity showed positive reaction on some of the transformation processed apical points and calli. This demonstrated that foreign genes were introduced into the apical meristematic cells as well as the callus cells. The plantlets of cv. “Jing 411” survived after apical point transformation with pBIAH A + were transplanted into the field and the progenies were screened with kanamycin. 4% of the screened seeds germinated into green seedlings with kanamycin resistance. Dot hybridization of total DNA from kanamycin resistant plants showed the existence of foreign DNA in some of the detected plants.展开更多
Automatic and accurate classification is a fundamental problem to the analysis and modeling of LiDAR(Light Detection and Ranging)data.Recently,convolutional neural network(ConvNet or CNN)has achieved remarkable perfor...Automatic and accurate classification is a fundamental problem to the analysis and modeling of LiDAR(Light Detection and Ranging)data.Recently,convolutional neural network(ConvNet or CNN)has achieved remarkable performance in image recognition and computer vision.While significant efforts have also been made to develop various deep networks for satellite image scene classification,it still needs to further investigate suitable deep learning network frameworks for 3D dense mobile laser scanning(MLS)data.In this paper,we present a simple deep CNN for multiple object classification based on multi-scale context representation.For the pointwise classification,we first extracted the neighboring points within spatial context and transformed them into a three-channel image for each point.Then,the classification task can be treated as the image recognition using CNN.The proposed CNN architecture adopted common convolution,maximum pooling and rectified linear unit(ReLU)layers,which combined multiple deeper network layers.After being trained and tested on approximately seven million labeled MLS points,the deep CNN model can classify accurately into nine classes.Comparing with the widely used ResNet algorithm,this model performs better precision and recall rates,and less processing time,which indicated the significant potential of deep-learning-based methods in MLS data classification.展开更多
文摘Apical points of young seedlings of wheat (Triticum aestivum) cultivar “Jing 411” and somatic calli of cultivar “FK8” were transformed with plasmids pBI121 and (or) pBIAH A + by using microprojectile bombardment. Histochemical assay of GUS activity showed positive reaction on some of the transformation processed apical points and calli. This demonstrated that foreign genes were introduced into the apical meristematic cells as well as the callus cells. The plantlets of cv. “Jing 411” survived after apical point transformation with pBIAH A + were transplanted into the field and the progenies were screened with kanamycin. 4% of the screened seeds germinated into green seedlings with kanamycin resistance. Dot hybridization of total DNA from kanamycin resistant plants showed the existence of foreign DNA in some of the detected plants.
基金National Natural Science Foundation of China(Nos.41971423,31972951,41771462)Hunan Provincial Natural Science Foundation of China(No.2020JJ3020)+1 种基金Science and Technology Planning Project of Hunan Province(No.2019RS2043,2019GK2132)Outstanding Youth Project of Education Department of Hunan Province(No.18B224)。
文摘Automatic and accurate classification is a fundamental problem to the analysis and modeling of LiDAR(Light Detection and Ranging)data.Recently,convolutional neural network(ConvNet or CNN)has achieved remarkable performance in image recognition and computer vision.While significant efforts have also been made to develop various deep networks for satellite image scene classification,it still needs to further investigate suitable deep learning network frameworks for 3D dense mobile laser scanning(MLS)data.In this paper,we present a simple deep CNN for multiple object classification based on multi-scale context representation.For the pointwise classification,we first extracted the neighboring points within spatial context and transformed them into a three-channel image for each point.Then,the classification task can be treated as the image recognition using CNN.The proposed CNN architecture adopted common convolution,maximum pooling and rectified linear unit(ReLU)layers,which combined multiple deeper network layers.After being trained and tested on approximately seven million labeled MLS points,the deep CNN model can classify accurately into nine classes.Comparing with the widely used ResNet algorithm,this model performs better precision and recall rates,and less processing time,which indicated the significant potential of deep-learning-based methods in MLS data classification.
