Peanut pod shape is a heritable trait which affects the market acceptance of in-shell peanut products.In order to determine the genetic control of pod shape,six component traits of pod shape(pod length,pod width,pod l...Peanut pod shape is a heritable trait which affects the market acceptance of in-shell peanut products.In order to determine the genetic control of pod shape,six component traits of pod shape(pod length,pod width,pod length/width ratio,pod roundness,beak degree and constriction degree)were measured using an image-based phenotyping method.A recombinant inbred line(RIL)population consisting of 181 lines was phenotyped across three environments.Continuous distributions and transgressive segregations were demonstrated in all measured traits and environments.Significant correlations were found among most component traits with broad-sense heritability ranging from 0.87 to 0.95.Quantitative trait locus(QTL)analysis yielded 26 additive QTLs explaining 3.79 to 52.37%phenotypic variations.A novel,stable and major QTL region conditioning multiple shape features was detected on chromosome 2,which spans a 10.81-Mb genomic region with 543 putative genes.Bioinformatics analysis revealed several candidate genes in this region.In addition,73 pairs of epistatic interactions involving 92 loci were identified for six component traits explaining 0.94–6.45%phenotypic variations.These results provide new genetic loci to facilitate genomics-assisted breeding of peanut pod shape.展开更多
基金supported by the National Natural Science Foundation of China(32001584)the Natural Science Foundation of Shandong Province,China(ZR202111290099)+2 种基金the Breeding Project from Department Science&Technology of Shandong Province,China(2020LZGC001)the Agricultural Scientific and the Technological Innovation Project of Shandong Academy of Agricultural Sciences,China(CXGC2022A21,CXGC2022A03,and CXGC2023A06(A39 and A46))the Qingdao People’s Livelihood Science and Technology Program,China(20-3-4-26-nsh)。
文摘Peanut pod shape is a heritable trait which affects the market acceptance of in-shell peanut products.In order to determine the genetic control of pod shape,six component traits of pod shape(pod length,pod width,pod length/width ratio,pod roundness,beak degree and constriction degree)were measured using an image-based phenotyping method.A recombinant inbred line(RIL)population consisting of 181 lines was phenotyped across three environments.Continuous distributions and transgressive segregations were demonstrated in all measured traits and environments.Significant correlations were found among most component traits with broad-sense heritability ranging from 0.87 to 0.95.Quantitative trait locus(QTL)analysis yielded 26 additive QTLs explaining 3.79 to 52.37%phenotypic variations.A novel,stable and major QTL region conditioning multiple shape features was detected on chromosome 2,which spans a 10.81-Mb genomic region with 543 putative genes.Bioinformatics analysis revealed several candidate genes in this region.In addition,73 pairs of epistatic interactions involving 92 loci were identified for six component traits explaining 0.94–6.45%phenotypic variations.These results provide new genetic loci to facilitate genomics-assisted breeding of peanut pod shape.
