Background: Clear tumor imaging is essential to the resection of hepatocellular carcinoma(HCC). This study aimed to create a novel biological probe to improve the HCC imaging. Methods: Au nano-flower particles and CuI...Background: Clear tumor imaging is essential to the resection of hepatocellular carcinoma(HCC). This study aimed to create a novel biological probe to improve the HCC imaging. Methods: Au nano-flower particles and CuInS2 –Zn S core-shell quantum dots were synthesized by hydrothermal method. Au was coated with porous SiO2 and combined with anti-AFP antibody. HCC cell line HepG2 was used to evaluate the targeting efficacy of the probe, while flow cytometry and MTT assay were used to detect the cytotoxicity and bio-compatibility of the probe. Probes were subcutaneously injected to nude mice to explore light intensity and tissue penetration. Results: The fluorescence stability of the probe was maintained 100% for 24 h, and the brightness value was 4 times stronger than that of the corresponding CuInS2 –Zn S quantum dot. In the targeting experiment, the labeled HepG2 emitted yellow fluorescence. In the cytotoxicity experiments, MTT and flow cytometry results showed that the bio-compatibility of the probe was fine, the inhibition rate of HepG2 cell with 60% Cu-QDs/Anti-AFP probe and Au-QDs/Anti-AFP probe solution for 48 h were significantly different(86.3%±7.0% vs. 4.9%±1.3%, t = 19.745, P < 0.05), and the apoptosis rates were 83.3%±5.1% vs. 4.4%±0.8%( P < 0.001). In the animal experiment, the luminescence of the novel probe can penetrate the abdominal tissues of a mouse, stronger than that of CuInS2 –ZnS quantum dot. Conclusions: The Au@SiO2 @CuInS2 –ZnS/Anti-AFP probe can targetedly recognize and label HepG2 cells with good bio-compatibility and no toxicity, and the strong tissue penetrability of luminescence may be helpful to surgeons.展开更多
Rubrene thin films are deposited on quartz substrates and silver nanoparticles （Ag NPs） films by the thermal evapo- ration technique. The optical properties of rubrene thin film are investigated in a spectral range ...Rubrene thin films are deposited on quartz substrates and silver nanoparticles （Ag NPs） films by the thermal evapo- ration technique. The optical properties of rubrene thin film are investigated in a spectral range of 190 nm-1600 nm. The analysis of the absorption coefficient （a） reveals direct allowed transition with a corresponding energy of 2.24 eV. The photoluminescence （PL） peak of the mbrene thin film is observed to be at 563 nm （2.21 eV）. With the use ofAg NPs which are fabricated by radio-frequency （RF） rnagnetron sputtering on the quartz, the PL intensity is 8.5 times that of as-deposited rubrene thin film. It is attributed to the fact that the surface plasmon enhances the photoluminescence.展开更多
A facile and rapid approach for detecting low concentration of iron ion(Fe3+) with improved sensitivity was developed on the basis of plasmon enhanced fluorescence and subsequently amplified fluorescence quenching.Au1...A facile and rapid approach for detecting low concentration of iron ion(Fe3+) with improved sensitivity was developed on the basis of plasmon enhanced fluorescence and subsequently amplified fluorescence quenching.Au1Ag4@Si O2 nanoparticles were synthesized and dispersed into fluorescein isothiocyanate(FITC) solution. The fluorescence of the FITC solution was improved due to plasmon enhanced fluorescence. However, efficient fluorescence quenching of the FITC/Au1Ag4@Si O2 solution was subsequently achieved when Fe3+, with a concentration ranging from17 n M to 3.4 l M, was added into the FITC/Au1Ag4@Si O2 solution, whereas almost no fluorescence quenching was observed for pure FITC solution under the same condition. FITC/Au1Ag4@Si O2 solution shows a better sensitivity for detecting low concentration of Fe3+compared to pure FITC solution. The quantized limit of detection toward Fe3+was improved from 4.6 l M for pure FITC solution to 20 n M for FITC/Au1Ag4@Si O2 solution.展开更多
Optical absorption in thin-film solar cells can be improved by using surface plasmons for guiding and confining the light on the nanoscale.We report theoretical and simulation studies of a-Si thin-film solar cells wit...Optical absorption in thin-film solar cells can be improved by using surface plasmons for guiding and confining the light on the nanoscale.We report theoretical and simulation studies of a-Si thin-film solar cells with silver nanocylinders on the surface.We found that surface plasmons increased the cells＇ spectral response over almost the entire studied solar spectrum.In the ultraviolet range and at wavelengths close to the Si band gap we observed a significant enhancement of the absorption for both thin-film and wafer-based structures.We also performed optimization studies of particle size,inter-particle distance,and dielectric environment,for obtaining maximal absorption within the substrate.A blue-shift of the resonance wavelength with increasing inter-particle distance was observed in the visible range.Cell performance improved at optimal spacing,which strongly depended on the nanoparticle size.Increasing the nanocylinder size was accompanied by the widening of the plasmon resonance band and a red-shift of the plasmon resonance peaks.A weak red-shift and plasmon peak enhancement were observed in the reflectance curve with increasing refractive index of the dielectric spacer.展开更多
便携式拉曼光谱仪在现场快速分析中往往由于现场样品比较复杂,需要处理后才能采用光谱仪进行测定,因此一定程度上限制它的使用。常规实验室前处理虽然行之有效,但是过程繁琐费时,不适合现场使用。本课题组开发了一种便携式快速PERS前处...便携式拉曼光谱仪在现场快速分析中往往由于现场样品比较复杂,需要处理后才能采用光谱仪进行测定,因此一定程度上限制它的使用。常规实验室前处理虽然行之有效,但是过程繁琐费时,不适合现场使用。本课题组开发了一种便携式快速PERS前处理仪器,具有便携、功耗低、操作简便,提取时间短以及重复性好等特点,能满足现场快速检测的前处理需求。该仪器由超声波提取单元、加热抽气挥发单元、尾气净化与吸收单元以及控制系统四个功能模块组成,并且设有LED控制面板和报警装置。整个工作流程主要包括超声提取、液液萃取和溶剂加热抽气挥发三个环节。以三种实际样品(辣椒粉、辣椒油、豆瓣酱)中的违禁添加剂罗丹明B检测为例,结果表明:与常规实验室前处理方法相比,两种前处理方法对于PERS谱图影响不大,但是检测时间缩短一半。另外,重复性实验检测结果的相对标准偏差(relative standard deviation,RSD)小于±5%。展开更多
基金supported by grants from the National Natu-ral Science Foundation of China(51672003 and 51872279)
文摘Background: Clear tumor imaging is essential to the resection of hepatocellular carcinoma(HCC). This study aimed to create a novel biological probe to improve the HCC imaging. Methods: Au nano-flower particles and CuInS2 –Zn S core-shell quantum dots were synthesized by hydrothermal method. Au was coated with porous SiO2 and combined with anti-AFP antibody. HCC cell line HepG2 was used to evaluate the targeting efficacy of the probe, while flow cytometry and MTT assay were used to detect the cytotoxicity and bio-compatibility of the probe. Probes were subcutaneously injected to nude mice to explore light intensity and tissue penetration. Results: The fluorescence stability of the probe was maintained 100% for 24 h, and the brightness value was 4 times stronger than that of the corresponding CuInS2 –Zn S quantum dot. In the targeting experiment, the labeled HepG2 emitted yellow fluorescence. In the cytotoxicity experiments, MTT and flow cytometry results showed that the bio-compatibility of the probe was fine, the inhibition rate of HepG2 cell with 60% Cu-QDs/Anti-AFP probe and Au-QDs/Anti-AFP probe solution for 48 h were significantly different(86.3%±7.0% vs. 4.9%±1.3%, t = 19.745, P < 0.05), and the apoptosis rates were 83.3%±5.1% vs. 4.4%±0.8%( P < 0.001). In the animal experiment, the luminescence of the novel probe can penetrate the abdominal tissues of a mouse, stronger than that of CuInS2 –ZnS quantum dot. Conclusions: The Au@SiO2 @CuInS2 –ZnS/Anti-AFP probe can targetedly recognize and label HepG2 cells with good bio-compatibility and no toxicity, and the strong tissue penetrability of luminescence may be helpful to surgeons.
基金Project supported by the Funding for the Development Project of Beijing Municipal Education Commission of Science and Technology,China(Grant No.KZ201410005008)the Natural Science Foundation of Beijing City,China(Grant No.4102014)the Graduate Science Fund of the Beijing University of Technology,China(Grant No.ykj-2013-9835)
文摘Rubrene thin films are deposited on quartz substrates and silver nanoparticles （Ag NPs） films by the thermal evapo- ration technique. The optical properties of rubrene thin film are investigated in a spectral range of 190 nm-1600 nm. The analysis of the absorption coefficient （a） reveals direct allowed transition with a corresponding energy of 2.24 eV. The photoluminescence （PL） peak of the mbrene thin film is observed to be at 563 nm （2.21 eV）. With the use ofAg NPs which are fabricated by radio-frequency （RF） rnagnetron sputtering on the quartz, the PL intensity is 8.5 times that of as-deposited rubrene thin film. It is attributed to the fact that the surface plasmon enhances the photoluminescence.
基金supported by the National Natural Science Foundation of China (51003069)Natural Science Foundation of Jiangsu Higher Education Institutions of China (10KJB430014)A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘A facile and rapid approach for detecting low concentration of iron ion(Fe3+) with improved sensitivity was developed on the basis of plasmon enhanced fluorescence and subsequently amplified fluorescence quenching.Au1Ag4@Si O2 nanoparticles were synthesized and dispersed into fluorescein isothiocyanate(FITC) solution. The fluorescence of the FITC solution was improved due to plasmon enhanced fluorescence. However, efficient fluorescence quenching of the FITC/Au1Ag4@Si O2 solution was subsequently achieved when Fe3+, with a concentration ranging from17 n M to 3.4 l M, was added into the FITC/Au1Ag4@Si O2 solution, whereas almost no fluorescence quenching was observed for pure FITC solution under the same condition. FITC/Au1Ag4@Si O2 solution shows a better sensitivity for detecting low concentration of Fe3+compared to pure FITC solution. The quantized limit of detection toward Fe3+was improved from 4.6 l M for pure FITC solution to 20 n M for FITC/Au1Ag4@Si O2 solution.
文摘Optical absorption in thin-film solar cells can be improved by using surface plasmons for guiding and confining the light on the nanoscale.We report theoretical and simulation studies of a-Si thin-film solar cells with silver nanocylinders on the surface.We found that surface plasmons increased the cells＇ spectral response over almost the entire studied solar spectrum.In the ultraviolet range and at wavelengths close to the Si band gap we observed a significant enhancement of the absorption for both thin-film and wafer-based structures.We also performed optimization studies of particle size,inter-particle distance,and dielectric environment,for obtaining maximal absorption within the substrate.A blue-shift of the resonance wavelength with increasing inter-particle distance was observed in the visible range.Cell performance improved at optimal spacing,which strongly depended on the nanoparticle size.Increasing the nanocylinder size was accompanied by the widening of the plasmon resonance band and a red-shift of the plasmon resonance peaks.A weak red-shift and plasmon peak enhancement were observed in the reflectance curve with increasing refractive index of the dielectric spacer.
文摘便携式拉曼光谱仪在现场快速分析中往往由于现场样品比较复杂,需要处理后才能采用光谱仪进行测定,因此一定程度上限制它的使用。常规实验室前处理虽然行之有效,但是过程繁琐费时,不适合现场使用。本课题组开发了一种便携式快速PERS前处理仪器,具有便携、功耗低、操作简便,提取时间短以及重复性好等特点,能满足现场快速检测的前处理需求。该仪器由超声波提取单元、加热抽气挥发单元、尾气净化与吸收单元以及控制系统四个功能模块组成,并且设有LED控制面板和报警装置。整个工作流程主要包括超声提取、液液萃取和溶剂加热抽气挥发三个环节。以三种实际样品(辣椒粉、辣椒油、豆瓣酱)中的违禁添加剂罗丹明B检测为例,结果表明:与常规实验室前处理方法相比,两种前处理方法对于PERS谱图影响不大,但是检测时间缩短一半。另外,重复性实验检测结果的相对标准偏差(relative standard deviation,RSD)小于±5%。
