Based on the target analysis of the operation optimization for power plants, a novel system scheme called operation optimization decision support system (OODSS) is brought forward. According to the structure and desig...Based on the target analysis of the operation optimization for power plants, a novel system scheme called operation optimization decision support system (OODSS) is brought forward. According to the structure and design thinking of decision support system (DSS), the overall structure of the OODSS is studied, and the scheme of the sub systems in the OODSS such as the user interface system, the problem processing system, the database system, the model base system, the expert system (ES) and the data mining sy...展开更多
The most popular CRISPR-SpCas9 systemrecognizes canonical NGG protospacer adjacent motifs(PAMs).Previously engineered SpCas9 variants,such as Cas9-NG,favor G-rich PAMs in genome editing.In this manuscript,we describe ...The most popular CRISPR-SpCas9 systemrecognizes canonical NGG protospacer adjacent motifs(PAMs).Previously engineered SpCas9 variants,such as Cas9-NG,favor G-rich PAMs in genome editing.In this manuscript,we describe a new plant genome-editing system based on a hybrid iSpyMacCas9 platform that allows for targeted mutagenesis,C to T base editing,and A to G base editing at A-rich PAMs.This study fills amajor technology gap in the CRISPR-Cas9 system for editing NAAR PAMs in plants,which greatly expands the targeting scope of CRISPR-Cas9.Finally,our vector systems are fully compatible with Gateway cloning and will work with all existing single-guide RNA expression systems,facilitating easy adoption of the systems by others.We anticipate that more tools,such as prime editing,homology-directed repair,CRISPR interference,and CRISPR activation,will be further developed based on our promising iSpyMac-Cas9 platform.展开更多
文摘Based on the target analysis of the operation optimization for power plants, a novel system scheme called operation optimization decision support system (OODSS) is brought forward. According to the structure and design thinking of decision support system (DSS), the overall structure of the OODSS is studied, and the scheme of the sub systems in the OODSS such as the user interface system, the problem processing system, the database system, the model base system, the expert system (ES) and the data mining sy...
基金supported by startup funds from the University of Maryland,the National Science Foundation Plant Genome Research Program grant(award no.IOS-1758745)the Biotechnology Risk Assessment Grant Program competitive grant(award no.2018-33522-28789)from the U.S.Department of Agriculture.
文摘The most popular CRISPR-SpCas9 systemrecognizes canonical NGG protospacer adjacent motifs(PAMs).Previously engineered SpCas9 variants,such as Cas9-NG,favor G-rich PAMs in genome editing.In this manuscript,we describe a new plant genome-editing system based on a hybrid iSpyMacCas9 platform that allows for targeted mutagenesis,C to T base editing,and A to G base editing at A-rich PAMs.This study fills amajor technology gap in the CRISPR-Cas9 system for editing NAAR PAMs in plants,which greatly expands the targeting scope of CRISPR-Cas9.Finally,our vector systems are fully compatible with Gateway cloning and will work with all existing single-guide RNA expression systems,facilitating easy adoption of the systems by others.We anticipate that more tools,such as prime editing,homology-directed repair,CRISPR interference,and CRISPR activation,will be further developed based on our promising iSpyMac-Cas9 platform.
