在海南的赛葵上发现了类似植原体感染的病症,症状表现为花变叶和叶片变小。本研究通过植原体16S r DNA通用引物P1/P7对表现症状的赛葵植株进行了PCR检测,并对检测到的植原体病原16S r DNA进行了克隆测序、序列比对、虚拟RFLP分析和系统...在海南的赛葵上发现了类似植原体感染的病症,症状表现为花变叶和叶片变小。本研究通过植原体16S r DNA通用引物P1/P7对表现症状的赛葵植株进行了PCR检测,并对检测到的植原体病原16S r DNA进行了克隆测序、序列比对、虚拟RFLP分析和系统进化树构建分析。结果表明,该植原体为翠菊黄化植原体候选种相关株系,属于翠菊黄化植原体组(16SrI)的B亚组(相似系数为1.00)。展开更多
Wheat blue dwarf(WBD)is a disease caused by phytoplasma and only reported from China.A fragment about 1.3 kb in protein translocation gene,secY was amplified by PCR from the total DNA of di-seased wheat sample with pr...Wheat blue dwarf(WBD)is a disease caused by phytoplasma and only reported from China.A fragment about 1.3 kb in protein translocation gene,secY was amplified by PCR from the total DNA of di-seased wheat sample with primer pair secYF/secYR,which was designed based on secY gene sequence of known 16SrI group members.Nucleotide acid sequence analysis of amplified fragment indicated that the length was 1 240 bp.A phylogenetic tree based on secY gene sequences was constructed and showed that wheat blue dwarf phytoplasma was clustered into the Candidatus Phytoplasma asteris,subgroup 16SrI-C.Wheat blue dwarf phytoplasma showed high homology with clover phyllody phytoplasma strains based on sequence comparison and phylogenetic analysis.展开更多
文摘在海南的赛葵上发现了类似植原体感染的病症,症状表现为花变叶和叶片变小。本研究通过植原体16S r DNA通用引物P1/P7对表现症状的赛葵植株进行了PCR检测,并对检测到的植原体病原16S r DNA进行了克隆测序、序列比对、虚拟RFLP分析和系统进化树构建分析。结果表明,该植原体为翠菊黄化植原体候选种相关株系,属于翠菊黄化植原体组(16SrI)的B亚组(相似系数为1.00)。
文摘Wheat blue dwarf(WBD)is a disease caused by phytoplasma and only reported from China.A fragment about 1.3 kb in protein translocation gene,secY was amplified by PCR from the total DNA of di-seased wheat sample with primer pair secYF/secYR,which was designed based on secY gene sequence of known 16SrI group members.Nucleotide acid sequence analysis of amplified fragment indicated that the length was 1 240 bp.A phylogenetic tree based on secY gene sequences was constructed and showed that wheat blue dwarf phytoplasma was clustered into the Candidatus Phytoplasma asteris,subgroup 16SrI-C.Wheat blue dwarf phytoplasma showed high homology with clover phyllody phytoplasma strains based on sequence comparison and phylogenetic analysis.
