Signal transducer and activator of transcription（STAT）is a unique protein family that binds to DNA,coupled with tyrosine phosphorylation signaling pathways,acting as a transcriptional regulator to mediate a variety ...Signal transducer and activator of transcription（STAT）is a unique protein family that binds to DNA,coupled with tyrosine phosphorylation signaling pathways,acting as a transcriptional regulator to mediate a variety of biological effects.Cerebral ischemia and reperfusion can activate STATs signaling pathway,but no studies have confirmed whether STAT activation can be verified by diffusion-weighted magnetic resonance imaging（DWI）in rats after cerebral ischemia/reperfusion.Here,we established a rat model of focal cerebral ischemia injury using the modified Longa method.DWI revealed hyperintensity in parts of the left hemisphere before reperfusion and a low apparent diffusion coefficient.STAT3 protein expression showed no significant change after reperfusion,but phosphorylated STAT3 expression began to increase after 30 minutes of reperfusion and peaked at 24 hours.Pearson correlation analysis showed that STAT3 activation was correlated positively with the relative apparent diffusion coefficient and negatively with the DWI abnormal signal area.These results indicate that DWI is a reliable representation of the infarct area and reflects STAT phosphorylation in rat brain following focal cerebral ischemia/reperfusion.展开更多
目的分析磷酸化转录信号转导子及激活子3(phosphorylated signal transducer and activator of transcription-3,p-STAT3)/磷酸化蛋白激酶B(phosphorylated protein kinase B,p-AKT)信号通路及其靶基因细胞周期素D1(cyclinD1)在基底细胞...目的分析磷酸化转录信号转导子及激活子3(phosphorylated signal transducer and activator of transcription-3,p-STAT3)/磷酸化蛋白激酶B(phosphorylated protein kinase B,p-AKT)信号通路及其靶基因细胞周期素D1(cyclinD1)在基底细胞癌(basal cell carcinoma,BCC)皮损中的表达及其临床意义。方法选取2010年1月—2019年8月在山东省济南市人民医院皮肤科就诊且经组织病理及免疫组化确诊为BCC的60例患者石蜡标本,以及在本院接受外科整形手术并留存的正常皮肤组织标本的患者,分别纳入BBC组及正常组。分别采用免疫组化法、PCR法、Western印迹实验检测皮损处p-STAT3、p-AKT及cyclinD1表达,并分析BCC患者皮损处p-STAT3 mRNA、p-AKT mRNA、cyclinD1 mRNA与临床病理的关系。结果BCC组p-STAT3、p-AKT、cyclinD1平均光密度值,p-STAT3 mRNA、p-AKT mRNA、cyclinD1 mRNA及p-STAT3蛋白、p-AKT蛋白、cyclinD1蛋白表达显著高于正常组(P<0.05);且IBCC型BCC患者皮损中p-STAT3 mRNA、p-AKT mRNA、cyclinD1 mRNA表达显著高于NIBCC患者(P<0.05);BCC皮损中p-STAT3 mRNA、p-AKT mRNA、cyclinD1 mRNA表达与病理类型有显著相关性(P<0.01)。结论BCC皮损处存在p-STAT3、p-AKT、cyclinD1高表达现象,且IBCC型BCC患者皮损处p-STAT3 mRNA、p-AKT mRNA、cyclinD1 mRNA表达更高,值得临床重视。展开更多
目的研究细胞因子信号转导抑制因子-3(suppressor of cytokine signaling-3,SOCS-3)在喉鳞状细胞癌(LSCC)组织中的表达,并进一步探讨SOCS-3与磷酸化信号转导转录活化因子-3(phosphorylation signal transducer and activator of transcr...目的研究细胞因子信号转导抑制因子-3(suppressor of cytokine signaling-3,SOCS-3)在喉鳞状细胞癌(LSCC)组织中的表达,并进一步探讨SOCS-3与磷酸化信号转导转录活化因子-3(phosphorylation signal transducer and activator of transcription 3,p-STAT3)的相关性及与喉癌临床病理因素的关系。方法收集2005年2月～2009年2月在白求恩国际和平医院耳鼻咽喉头颈外科接受手术的LSCC石蜡标本40例,并选取11例正常喉黏膜组织作为对照。采用免疫组化染色法检测SOCS-3、p-STAT3蛋白的表达情况。结果①LSCC组织中SCOS-3蛋白表达明显低于正常喉黏膜组织,两者之间差异具有统计学意义(P=0.000);p-STAT3蛋白在正常喉黏膜组织中的表达小于喉癌组织(P=0.000);②LSCC组织中SOCS-3与p-STAT3蛋白之间存在显著负相关(r=-0.459,P=0.003);③SOCS-3表达与患者年龄(P=0.223)、性别(P=0.849)、肿瘤T分级(P=0.193)无相关性,而与肿瘤病理分期(P=0.0 0 0),淋巴结转移(P=0.0 0 0)有关。结论①在喉正常黏膜组织中SOCS-3均呈阳性表达,而在LSCC组织中部分呈阳性表达;并与p-STAT3蛋白的表达情况呈负相关。提示SOCS-3可能在喉癌生长的过程通过抑制JAK/STAT3信号转导通路的持续活化从而抑制喉癌细胞增长,促进细胞凋亡;②SOCS-3的表达与患者年龄、性别、肿瘤T分级无相关性,而与喉癌病理分期及颈部淋巴结转移有关。展开更多
文摘Signal transducer and activator of transcription（STAT）is a unique protein family that binds to DNA,coupled with tyrosine phosphorylation signaling pathways,acting as a transcriptional regulator to mediate a variety of biological effects.Cerebral ischemia and reperfusion can activate STATs signaling pathway,but no studies have confirmed whether STAT activation can be verified by diffusion-weighted magnetic resonance imaging（DWI）in rats after cerebral ischemia/reperfusion.Here,we established a rat model of focal cerebral ischemia injury using the modified Longa method.DWI revealed hyperintensity in parts of the left hemisphere before reperfusion and a low apparent diffusion coefficient.STAT3 protein expression showed no significant change after reperfusion,but phosphorylated STAT3 expression began to increase after 30 minutes of reperfusion and peaked at 24 hours.Pearson correlation analysis showed that STAT3 activation was correlated positively with the relative apparent diffusion coefficient and negatively with the DWI abnormal signal area.These results indicate that DWI is a reliable representation of the infarct area and reflects STAT phosphorylation in rat brain following focal cerebral ischemia/reperfusion.
文摘目的研究细胞因子信号转导抑制因子-3(suppressor of cytokine signaling-3,SOCS-3)在喉鳞状细胞癌(LSCC)组织中的表达,并进一步探讨SOCS-3与磷酸化信号转导转录活化因子-3(phosphorylation signal transducer and activator of transcription 3,p-STAT3)的相关性及与喉癌临床病理因素的关系。方法收集2005年2月～2009年2月在白求恩国际和平医院耳鼻咽喉头颈外科接受手术的LSCC石蜡标本40例,并选取11例正常喉黏膜组织作为对照。采用免疫组化染色法检测SOCS-3、p-STAT3蛋白的表达情况。结果①LSCC组织中SCOS-3蛋白表达明显低于正常喉黏膜组织,两者之间差异具有统计学意义(P=0.000);p-STAT3蛋白在正常喉黏膜组织中的表达小于喉癌组织(P=0.000);②LSCC组织中SOCS-3与p-STAT3蛋白之间存在显著负相关(r=-0.459,P=0.003);③SOCS-3表达与患者年龄(P=0.223)、性别(P=0.849)、肿瘤T分级(P=0.193)无相关性,而与肿瘤病理分期(P=0.0 0 0),淋巴结转移(P=0.0 0 0)有关。结论①在喉正常黏膜组织中SOCS-3均呈阳性表达,而在LSCC组织中部分呈阳性表达;并与p-STAT3蛋白的表达情况呈负相关。提示SOCS-3可能在喉癌生长的过程通过抑制JAK/STAT3信号转导通路的持续活化从而抑制喉癌细胞增长,促进细胞凋亡;②SOCS-3的表达与患者年龄、性别、肿瘤T分级无相关性,而与喉癌病理分期及颈部淋巴结转移有关。
