Peroxiredoxin-6(PRDX6)is an antioxidant enzyme with both the activities of peroxidase and phospholipase A2(PLA2),which is involved in regulation of many cellular reactions.However,the function of PRDX6 during virus in...Peroxiredoxin-6(PRDX6)is an antioxidant enzyme with both the activities of peroxidase and phospholipase A2(PLA2),which is involved in regulation of many cellular reactions.However,the function of PRDX6 during virus infection remains unknown.In this study,we found that the abundance of PRDX6 protein was dramatically decreased in foot-and-mouth disease virus(FMDV)infected cells.Overexpression of PRDX6 inhibited FMDV replication.In contrast,knockdown of PRDX6 expression promoted FMDV replication,suggesting an antiviral role of PRDX6.To explore whether the activity of peroxidase and PLA2 was associated with PRDX6-mediated antiviral function,a specific inhibitor of PLA2(MJ33)and a specific inhibitor of peroxidase activity(mercaptosuccinate)were used to treat the cells before FMDV infection.The results showed that incubation of MJ33 but not mercaptosuccinate promoted FMDV replication.Meanwhile,overexpression of PRDX6 slightly enhanced type I interferon signaling.We further determined that the viral 3Cprowas responsible for degradation of PRDX6,and 3Cpro-induced reduction of PRDX6 was independent of the proteasome,lysosome,and caspase pathways.The protease activity of 3Cprowas required for induction of PRDX6 reduction.Besides,PRDX6 suppressed the replication of another porcine picornavirus Senecavirus A(SVA),and the 3Cproof SVA induced the reduction of PRDX6 through its proteolytic activity as well.Together,our results suggested that PRDX6 plays an important antiviral role during porcine picornavirus infection,and the viral 3Cproinduces the degradation of PRDX6 to overcome PRDX6-mediated antiviral function.展开更多
目的探讨肿瘤自身抗体NY-ESO-1、peroxiredoxin 6(PRDX6)和热休克蛋白70(heat shock protein 70,HSP70)联合检测在肺癌诊断中的应用价值。方法采用ELISA法检测92例肺癌患者(肺癌组)和80例体检健康者(对照组)血清NY-ESO-1、PRDX6和HSP70...目的探讨肿瘤自身抗体NY-ESO-1、peroxiredoxin 6(PRDX6)和热休克蛋白70(heat shock protein 70,HSP70)联合检测在肺癌诊断中的应用价值。方法采用ELISA法检测92例肺癌患者(肺癌组)和80例体检健康者(对照组)血清NY-ESO-1、PRDX6和HSP70表达水平,应用ROC曲线分析3项自身抗体联合诊断肺癌的效能。结果肺癌组血清NY-ESO-1[0.206(0.132,0.361)]、PRDX6(0.140±0.078)和HSP70(0.105±0.087)自身抗体水平明显高于对照组[0.095(0.055,0.145)、0.094±0.038、0.059±0.035],差异有统计学意义(P<0.01);血清NY-ESO-1自身抗体水平在年龄>60岁肺癌患者[0.325(0.161,0.683)]高于年龄≤60岁者[0.163(0.118,0.241)],有淋巴结转移者[0.229(0.132,0.431)]高于无淋巴结转移者[0.160(0.102,0.207)](P<0.05),在性别、组织病理类型和TNM分期上差异无统计学意义(P>0.05);血清PRDX6和HSP70自身抗体水平在年龄、性别、有无淋巴结转移、组织病理类型和TNM分期上差异均无统计学意义(P>0.05);血清NY-ESO-1、PRDX6和HSP70自身抗体诊断肺癌的敏感度分别为54.3%、30.4%、26.1%,特异度分别为92.5%、92.5%、92.5%;3种自身抗体联合检测诊断肺癌的敏感度为63.0%,特异度为91.2%,诊断敏感度高于单项指标检测,特异度略下降但差异无统计学意义(P<0.05)。结论血清NY-ESO-1、PRDX6和HSP70自身抗体联合检测诊断肺癌具有较高的敏感性,有可能用于肺癌的筛查和诊断。展开更多
基金supported by grants from the National Key R&D Program of China(2017YFD0501103)the Key Development and Research Foundation of Yunnan(2018BB004)+1 种基金the Chinese Academy of Agricultural Science and Technology Innovation Project(Y2017JC55)Central Public-interest Scientific Institution Basal Research Fund(1610312016013 and 1610312017003)。
文摘Peroxiredoxin-6(PRDX6)is an antioxidant enzyme with both the activities of peroxidase and phospholipase A2(PLA2),which is involved in regulation of many cellular reactions.However,the function of PRDX6 during virus infection remains unknown.In this study,we found that the abundance of PRDX6 protein was dramatically decreased in foot-and-mouth disease virus(FMDV)infected cells.Overexpression of PRDX6 inhibited FMDV replication.In contrast,knockdown of PRDX6 expression promoted FMDV replication,suggesting an antiviral role of PRDX6.To explore whether the activity of peroxidase and PLA2 was associated with PRDX6-mediated antiviral function,a specific inhibitor of PLA2(MJ33)and a specific inhibitor of peroxidase activity(mercaptosuccinate)were used to treat the cells before FMDV infection.The results showed that incubation of MJ33 but not mercaptosuccinate promoted FMDV replication.Meanwhile,overexpression of PRDX6 slightly enhanced type I interferon signaling.We further determined that the viral 3Cprowas responsible for degradation of PRDX6,and 3Cpro-induced reduction of PRDX6 was independent of the proteasome,lysosome,and caspase pathways.The protease activity of 3Cprowas required for induction of PRDX6 reduction.Besides,PRDX6 suppressed the replication of another porcine picornavirus Senecavirus A(SVA),and the 3Cproof SVA induced the reduction of PRDX6 through its proteolytic activity as well.Together,our results suggested that PRDX6 plays an important antiviral role during porcine picornavirus infection,and the viral 3Cproinduces the degradation of PRDX6 to overcome PRDX6-mediated antiviral function.
文摘目的探讨肿瘤自身抗体NY-ESO-1、peroxiredoxin 6(PRDX6)和热休克蛋白70(heat shock protein 70,HSP70)联合检测在肺癌诊断中的应用价值。方法采用ELISA法检测92例肺癌患者(肺癌组)和80例体检健康者(对照组)血清NY-ESO-1、PRDX6和HSP70表达水平,应用ROC曲线分析3项自身抗体联合诊断肺癌的效能。结果肺癌组血清NY-ESO-1[0.206(0.132,0.361)]、PRDX6(0.140±0.078)和HSP70(0.105±0.087)自身抗体水平明显高于对照组[0.095(0.055,0.145)、0.094±0.038、0.059±0.035],差异有统计学意义(P<0.01);血清NY-ESO-1自身抗体水平在年龄>60岁肺癌患者[0.325(0.161,0.683)]高于年龄≤60岁者[0.163(0.118,0.241)],有淋巴结转移者[0.229(0.132,0.431)]高于无淋巴结转移者[0.160(0.102,0.207)](P<0.05),在性别、组织病理类型和TNM分期上差异无统计学意义(P>0.05);血清PRDX6和HSP70自身抗体水平在年龄、性别、有无淋巴结转移、组织病理类型和TNM分期上差异均无统计学意义(P>0.05);血清NY-ESO-1、PRDX6和HSP70自身抗体诊断肺癌的敏感度分别为54.3%、30.4%、26.1%,特异度分别为92.5%、92.5%、92.5%;3种自身抗体联合检测诊断肺癌的敏感度为63.0%,特异度为91.2%,诊断敏感度高于单项指标检测,特异度略下降但差异无统计学意义(P<0.05)。结论血清NY-ESO-1、PRDX6和HSP70自身抗体联合检测诊断肺癌具有较高的敏感性,有可能用于肺癌的筛查和诊断。
