Objective:To facilitate the recognition of intracellular yeasts of Histoplasma capsulation and differentiate it from Leishmania amastigotes and other parasites,using the combination of Giemsa and a rapid modification ...Objective:To facilitate the recognition of intracellular yeasts of Histoplasma capsulation and differentiate it from Leishmania amastigotes and other parasites,using the combination of Giemsa and a rapid modification of Grocott stains to peripheral blood smears in a hematological study.Methods:The combination of both stains was applied consecutively(first Grocott and then Giemsa)to previously fixed peripheral blood smears.Microscopy was performed with 400x and 1000x,the latter using immersion oil.Results:The yeasts of Histoplasma capsulatum were observed into the cytoplasm of leukocytes as brownish oval elements,with 3-4μm in diameter.Conclusions:The combination of both techniques is a simple and fast method to facilitate recognition of intracellular yeasts and it is different from intracellular parasitic elements.Moreover,it allows distinguishing the cell elements that are in the microscopic preparations.It may be very helpful in those cases in which the presumptive diagnosis of histoplasmosis has not been established yet and where other more sophisticated methods are not available.展开更多
基金Supported by grant from the Louis Albert Foundation,Tolosa,Argentina(Grant No.165381)
文摘Objective:To facilitate the recognition of intracellular yeasts of Histoplasma capsulation and differentiate it from Leishmania amastigotes and other parasites,using the combination of Giemsa and a rapid modification of Grocott stains to peripheral blood smears in a hematological study.Methods:The combination of both stains was applied consecutively(first Grocott and then Giemsa)to previously fixed peripheral blood smears.Microscopy was performed with 400x and 1000x,the latter using immersion oil.Results:The yeasts of Histoplasma capsulatum were observed into the cytoplasm of leukocytes as brownish oval elements,with 3-4μm in diameter.Conclusions:The combination of both techniques is a simple and fast method to facilitate recognition of intracellular yeasts and it is different from intracellular parasitic elements.Moreover,it allows distinguishing the cell elements that are in the microscopic preparations.It may be very helpful in those cases in which the presumptive diagnosis of histoplasmosis has not been established yet and where other more sophisticated methods are not available.
