用气相色谱(GC)分析了紫苏油的脂肪酸组成,用高效液相色谱(HPLC)测定了紫苏油中维生素E含量,并对紫苏油的理化性质进行了研究。结果表明,紫苏油中不饱和脂肪酸含量为93.707%,其中亚麻酸含量最高,为60.752%,亚油酸和油酸含量分别为15.761...用气相色谱(GC)分析了紫苏油的脂肪酸组成,用高效液相色谱(HPLC)测定了紫苏油中维生素E含量,并对紫苏油的理化性质进行了研究。结果表明,紫苏油中不饱和脂肪酸含量为93.707%,其中亚麻酸含量最高,为60.752%,亚油酸和油酸含量分别为15.761%和17.194%;紫苏油中维生素E的总含量为500.9 m g/kg;紫苏油比重为0.928 8,折光指数为1.481 7,酸价为2.9 m g/g,碘价为1 980 g/kg,皂化值为190.7 m g/g,不皂化物为0.6%,水分及挥发物为0.07%,过氧化值为3.7 mm o l/kg。紫苏油是一种极具开发潜力的营养保健油。展开更多
In this study, production of ct-linoleic acid concentrated from crude perilla oil by gradient cooling urea inclusion was optimized. The fatty acid composition was determined after ethyl esterification by gas chromatog...In this study, production of ct-linoleic acid concentrated from crude perilla oil by gradient cooling urea inclusion was optimized. The fatty acid composition was determined after ethyl esterification by gas chromatography （GC）. In this process, orthogonal experiment was carried out. Under optimum conditions, the maximum amount of α-linoleic acid （91.5%） was obtained at a urea to fatty acid ratio of 3, a solvent to fatty acids ratio of 7, a reaction temperature of 348 K and a crystallization time of 690 min. A simple method of gradient cooling urea inclusion was used to purify α-linolenic acid by using urea to form inclusion complexes with the saturated and the less unsaturated fatty acids, which enhanced the purity of α-linoleic acid ethyl ester by above 90%.展开更多
采用大豆分离蛋白(Soybean protein isolate,SPI)-磷脂酰胆碱作为表面活性剂,利用超高压均质技术制备紫苏油纳米乳液,探究不同SPI质量分数以及超高压均质压力对紫苏油纳米乳液稳定性的影响。结果表明:随着SPI质量分数和超高压均质压力...采用大豆分离蛋白(Soybean protein isolate,SPI)-磷脂酰胆碱作为表面活性剂,利用超高压均质技术制备紫苏油纳米乳液,探究不同SPI质量分数以及超高压均质压力对紫苏油纳米乳液稳定性的影响。结果表明:随着SPI质量分数和超高压均质压力的增加,紫苏油纳米乳液的平均粒径和PDI值逐渐降低,在SPI质量分数为4%、均质压力140 MPa条件下紫苏油纳米乳液均一稳定,粒径最小为241.03 nm,PDI值最低为0.13;经过21 d的储藏试验,用Turbiscan稳定性分析仪测定紫苏油纳米乳液的物理稳定性,在SPI质量分数4%、均质压力140 MPa条件下,紫苏油纳米乳液未发生脂肪上浮或溶液聚集等现象,同时纳米乳液的平均粒径也未发生较大的变化,表明紫苏油纳米乳液在这一条件下具有较强的储藏稳定性。通过圆二色谱测定紫苏油纳米乳液中界面蛋白的二级结构变化,结果表明,超高压均质使α-螺旋的相对含量降低,而无规卷曲和β-折叠的相对含量升高,这说明界面蛋白的二级结构的变化对紫苏油纳米乳液的稳定性有一定的影响。展开更多
文摘用气相色谱(GC)分析了紫苏油的脂肪酸组成,用高效液相色谱(HPLC)测定了紫苏油中维生素E含量,并对紫苏油的理化性质进行了研究。结果表明,紫苏油中不饱和脂肪酸含量为93.707%,其中亚麻酸含量最高,为60.752%,亚油酸和油酸含量分别为15.761%和17.194%;紫苏油中维生素E的总含量为500.9 m g/kg;紫苏油比重为0.928 8,折光指数为1.481 7,酸价为2.9 m g/g,碘价为1 980 g/kg,皂化值为190.7 m g/g,不皂化物为0.6%,水分及挥发物为0.07%,过氧化值为3.7 mm o l/kg。紫苏油是一种极具开发潜力的营养保健油。
基金the National Natural Science Foundation of China (20871061)the National 973 Program of China (2008CB617512) for the financial support
文摘In this study, production of ct-linoleic acid concentrated from crude perilla oil by gradient cooling urea inclusion was optimized. The fatty acid composition was determined after ethyl esterification by gas chromatography （GC）. In this process, orthogonal experiment was carried out. Under optimum conditions, the maximum amount of α-linoleic acid （91.5%） was obtained at a urea to fatty acid ratio of 3, a solvent to fatty acids ratio of 7, a reaction temperature of 348 K and a crystallization time of 690 min. A simple method of gradient cooling urea inclusion was used to purify α-linolenic acid by using urea to form inclusion complexes with the saturated and the less unsaturated fatty acids, which enhanced the purity of α-linoleic acid ethyl ester by above 90%.
