目的:分析血清铁蛋白(SF)、脂滴包被蛋白、瘦素与妊娠期糖尿病(GDM)患者妊娠结局的相关性。方法:选取2017年10月至2019年12月在保定市第四中心医院进行产检且住院分娩的126例GDM患者为GDM组,同期82例正常妊娠孕妇为对照组。比较两组SF...目的:分析血清铁蛋白(SF)、脂滴包被蛋白、瘦素与妊娠期糖尿病(GDM)患者妊娠结局的相关性。方法:选取2017年10月至2019年12月在保定市第四中心医院进行产检且住院分娩的126例GDM患者为GDM组,同期82例正常妊娠孕妇为对照组。比较两组SF、脂滴包被蛋白、瘦素水平,比较GDM组不同血糖控制患者、不同妊娠结局患者SF、脂滴包被蛋白、瘦素水平的差异,分析GDM患者SF、脂滴包被蛋白、瘦素水平与空腹血糖(FPG)、餐后2 h血糖(2 h PG)水平和胰岛素抵抗指数(HOMA-IR)的关系及其预测GDM患者发生不良妊娠结局的价值。结果:GDM组SF、脂滴包被蛋白、瘦素水平高于对照组[(152.48±37.64)μg/L比(109.27±32.16)μg/L、(857.06±192.35)ng/L比(262.83±104.7)ng/L、(23.54±2.28)μg/L比(14.62±1.83)μg/L](P<0.05);GDM组血糖控制良好孕妇SF、脂滴包被蛋白、瘦素水平低于血糖控制不良孕妇[(132.10±36.52)μg/L比(176.37±40.06)μg/L、(176.37±40.06)ng/L比(946.42±205.37)ng/L、(21.49±2.16)μg/L比(25.94±2.40)μg/L](P<0.05);GDM组SF、脂滴包被蛋白、瘦素水平与FPG、2 h PG、HOMA-IR水平呈正相关(P<0.05);GDM组发生不良妊娠结局孕妇SF、脂滴包被蛋白、瘦素水平高于未发生患者[(182.86±42.29)μg/L比(138.86±35.47)μg/L、(1013.35±216.07)ng/L比(787.00±183.49)ng/L、(27.04±2.5)μg/L比(21.97±2.07)μg/L](P<0.05);Logistic回归分析显示,SF、脂滴包被蛋白、瘦素水平升高均是GDM患者发生不良妊娠结局的危险因素(P<0.05);SF、脂滴包被蛋白、瘦素联合预测GDM患者发生不良妊娠结局的灵敏度为76.92%,特异度为83.91%。结论:GDM患者SF、脂滴包被蛋白、瘦素呈异常高水平,且与血糖水平、HOMA-IR呈正相关,联合检测可提高对不良妊娠结局的预测价值。展开更多
Background:Nonalcoholic fatty liver disease(NAFLD)is one of the most common chronic liver diseases globally.Hepatic stellate cells(HSCs)are the major effector cells of liver fibrosis.HSCs contain abundant lipid drople...Background:Nonalcoholic fatty liver disease(NAFLD)is one of the most common chronic liver diseases globally.Hepatic stellate cells(HSCs)are the major effector cells of liver fibrosis.HSCs contain abundant lipid droplets(LDs)in their cytoplasm during quiescence.Perilipin 5(PLIN 5)is a LD surface-associated protein that plays a crucial role in lipid homeostasis.However,little is known about the role of PLIN 5 in HSC activation.Methods:PLIN 5 was overexpressed in HSCs of Sprague–Dawley rats by lentivirus transfection.At the same time,PLIN 5 gene knockout mice were constructed and fed with a high-fat diet(HFD)for 20 weeks to study the role of PLIN 5 in NAFLD.The corresponding reagent kits were used to measure TG,GSH,Caspase 3 activity,ATP level,and mitochondrial DNA copy number.Metabolomic analysis of mice liver tissue metabolism was performed based on UPLC-MS/MS.AMPK,mitochondrial function,cell proliferation,and apoptosis-related genes and proteins were detected by western blotting and qPCR.Results:Overexpression of PLIN 5 in activated HSCs led to a decrease in ATP levels in mitochondria,inhibition of cell proliferation,and a significant increase in cell apoptosis through AMPK activation.In addition,compared with the HFD-fed C57BL/6J mice,PLIN 5 knockout mice fed with HFD showed reduced liver fat deposition,decreased LD abundance and size,and reduced liver fibrosis.Conclusion:These findings highlight the unique regulatory role of PLIN 5 in HSCs and the role of PLIN 5 in the fibrosis process of NAFLD.展开更多
Hepatitis B virus(HBV)infection poses a global health concern without a definitive cure;however,antiviral medications can effectively suppress viral replication.This study delves into the intricate interplay between l...Hepatitis B virus(HBV)infection poses a global health concern without a definitive cure;however,antiviral medications can effectively suppress viral replication.This study delves into the intricate interplay between lipid metabo-lism and HBV replication,implicating molecular mechanisms such as the stearoyl coenzyme A desaturase 1 autophagy pathway,SAC1-like phosphatidylinositol phosphatase,and galectin-9 mediated selective autophagy of viral core proteins in regulating HBV replication.Within lipid droplets,perilipin 2(PLIN2)emerges as a pivotal guardian,with its overexpression protecting against autophagy and downregulation stimulating triglyceride catabolism through the autophagy pathway.This editorial discusses the correlation between hepatic steatosis and HBV replication,emphasizing the role of PLIN2 in this process.The study underscores the multifaceted roles of lipid metabolism,autophagy,and perilipins in HBV replication,shedding light on potential therapeutic avenues.展开更多
文摘目的:分析血清铁蛋白(SF)、脂滴包被蛋白、瘦素与妊娠期糖尿病(GDM)患者妊娠结局的相关性。方法:选取2017年10月至2019年12月在保定市第四中心医院进行产检且住院分娩的126例GDM患者为GDM组,同期82例正常妊娠孕妇为对照组。比较两组SF、脂滴包被蛋白、瘦素水平,比较GDM组不同血糖控制患者、不同妊娠结局患者SF、脂滴包被蛋白、瘦素水平的差异,分析GDM患者SF、脂滴包被蛋白、瘦素水平与空腹血糖(FPG)、餐后2 h血糖(2 h PG)水平和胰岛素抵抗指数(HOMA-IR)的关系及其预测GDM患者发生不良妊娠结局的价值。结果:GDM组SF、脂滴包被蛋白、瘦素水平高于对照组[(152.48±37.64)μg/L比(109.27±32.16)μg/L、(857.06±192.35)ng/L比(262.83±104.7)ng/L、(23.54±2.28)μg/L比(14.62±1.83)μg/L](P<0.05);GDM组血糖控制良好孕妇SF、脂滴包被蛋白、瘦素水平低于血糖控制不良孕妇[(132.10±36.52)μg/L比(176.37±40.06)μg/L、(176.37±40.06)ng/L比(946.42±205.37)ng/L、(21.49±2.16)μg/L比(25.94±2.40)μg/L](P<0.05);GDM组SF、脂滴包被蛋白、瘦素水平与FPG、2 h PG、HOMA-IR水平呈正相关(P<0.05);GDM组发生不良妊娠结局孕妇SF、脂滴包被蛋白、瘦素水平高于未发生患者[(182.86±42.29)μg/L比(138.86±35.47)μg/L、(1013.35±216.07)ng/L比(787.00±183.49)ng/L、(27.04±2.5)μg/L比(21.97±2.07)μg/L](P<0.05);Logistic回归分析显示,SF、脂滴包被蛋白、瘦素水平升高均是GDM患者发生不良妊娠结局的危险因素(P<0.05);SF、脂滴包被蛋白、瘦素联合预测GDM患者发生不良妊娠结局的灵敏度为76.92%,特异度为83.91%。结论:GDM患者SF、脂滴包被蛋白、瘦素呈异常高水平,且与血糖水平、HOMA-IR呈正相关,联合检测可提高对不良妊娠结局的预测价值。
基金Discipline Key Special ProjectGrant/Award Number:XKZDQY202001+7 种基金Henan Provincial Key R&D and Promotion Special ProjectGrant/Award Number:212102310033Henan Provincial Medical Science and Technology Tackling ProgramGrant/Award Number:LHGJ20220557Key R&D Program of ChinaGrant/Award Number:2020YFC2006100,2020YFC2009000 and 2020YFC2009006National Natural Science Foundation of ChinaGrant/Award Number:31471330 and 81870408。
文摘Background:Nonalcoholic fatty liver disease(NAFLD)is one of the most common chronic liver diseases globally.Hepatic stellate cells(HSCs)are the major effector cells of liver fibrosis.HSCs contain abundant lipid droplets(LDs)in their cytoplasm during quiescence.Perilipin 5(PLIN 5)is a LD surface-associated protein that plays a crucial role in lipid homeostasis.However,little is known about the role of PLIN 5 in HSC activation.Methods:PLIN 5 was overexpressed in HSCs of Sprague–Dawley rats by lentivirus transfection.At the same time,PLIN 5 gene knockout mice were constructed and fed with a high-fat diet(HFD)for 20 weeks to study the role of PLIN 5 in NAFLD.The corresponding reagent kits were used to measure TG,GSH,Caspase 3 activity,ATP level,and mitochondrial DNA copy number.Metabolomic analysis of mice liver tissue metabolism was performed based on UPLC-MS/MS.AMPK,mitochondrial function,cell proliferation,and apoptosis-related genes and proteins were detected by western blotting and qPCR.Results:Overexpression of PLIN 5 in activated HSCs led to a decrease in ATP levels in mitochondria,inhibition of cell proliferation,and a significant increase in cell apoptosis through AMPK activation.In addition,compared with the HFD-fed C57BL/6J mice,PLIN 5 knockout mice fed with HFD showed reduced liver fat deposition,decreased LD abundance and size,and reduced liver fibrosis.Conclusion:These findings highlight the unique regulatory role of PLIN 5 in HSCs and the role of PLIN 5 in the fibrosis process of NAFLD.
文摘Hepatitis B virus(HBV)infection poses a global health concern without a definitive cure;however,antiviral medications can effectively suppress viral replication.This study delves into the intricate interplay between lipid metabo-lism and HBV replication,implicating molecular mechanisms such as the stearoyl coenzyme A desaturase 1 autophagy pathway,SAC1-like phosphatidylinositol phosphatase,and galectin-9 mediated selective autophagy of viral core proteins in regulating HBV replication.Within lipid droplets,perilipin 2(PLIN2)emerges as a pivotal guardian,with its overexpression protecting against autophagy and downregulation stimulating triglyceride catabolism through the autophagy pathway.This editorial discusses the correlation between hepatic steatosis and HBV replication,emphasizing the role of PLIN2 in this process.The study underscores the multifaceted roles of lipid metabolism,autophagy,and perilipins in HBV replication,shedding light on potential therapeutic avenues.
