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奥曲肽对缺氧环境下大鼠胰腺外分泌细胞增殖、氧耗及HIF-1α、VEGF表达的影响
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作者 刘森峰 冯樾 +3 位作者 王圣禹 冯逸飞 莫庆辉 田磊 《广西医科大学学报》 CAS 2021年第5期887-892,共6页
目的:探究奥曲肽(Oct)在胰岛移植早期缺氧环境下对胰腺外分泌细胞AR42J的影响及可能机制。方法:将胰岛β细胞Min6分为常氧培养组和缺氧培养组,CCK-8法检测Min6细胞在常氧、缺氧环境中的增殖活性以确定培养时间。AR42J细胞分为常氧阴性... 目的:探究奥曲肽(Oct)在胰岛移植早期缺氧环境下对胰腺外分泌细胞AR42J的影响及可能机制。方法:将胰岛β细胞Min6分为常氧培养组和缺氧培养组,CCK-8法检测Min6细胞在常氧、缺氧环境中的增殖活性以确定培养时间。AR42J细胞分为常氧阴性对照组、常氧+Oct组、缺氧阴性对照组、缺氧+Oct组,将Oct稀释为不同浓度(10-6 mol/L、10-7 mol/L、10-8 mol/L、10-9 mol/L、10-10 mol/L)处理细胞。CCK-8法检测常氧、缺氧条件下Oct对AR42J增殖活性的影响,酶联免疫吸附试验(ELISA)法检测AR42J细胞培养上清液中血管内皮生长因子(VEGF)含量,磷氧探针检测细胞耗氧量,Western blotting法检测缺氧诱导因子(HIF-1α)蛋白表达。结果:Min6细胞在培养9 h后增殖活性达到最佳。与缺氧阴性对照组比较,缺氧+Oct组在作用3 h、6 h、9 h细胞活力上升(P<0.05)。与缺氧阴性对照组比较,除10-10 mol/L Oct组细胞HIF-1α表达无明显差异(P>0.05)外,其他各浓度Oct组HIF-1α蛋白表达量显著下降(均P<0.05)。与常氧阴性对照组比较,各浓度Oct组HIF-1α蛋白表达量均显著降低(均P<0.05)。与缺氧阴性对照组比较,缺氧+Oct组细胞上清VEGF含量和耗氧量降低(均P<0.05)。与常氧阴性对照组比较,各加药组HIF-1α表达降低(P<0.05),而VEGF含量、细胞耗氧量无明显差异(P>0.05)。结论:Oct提高缺氧环境下胰腺外分泌细胞活力,抑制HIF-1α与VEGF,降低细胞耗氧量,这可能是Oct对胰岛移植后缺氧环境中的胰岛细胞起到保护作用的机制之一。 展开更多
关键词 奥曲肽 胰腺外分泌细胞 增殖 VEGF HIF-1α
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DNA Content in Pancreatic ExocrinalCells after Vagotom yand Electron Microscopy in Rats 被引量:1
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作者 LI Jinsong, CHEN Daoda , TIAN Yuan Department of Surgery, Xiehe Hospital, Tongji Medical University, Wuhan 430022 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 1999年第3期226-229,共4页
Fifty six SD rats were randomly divided into the normal control group and the operation group. The operating group was subdivided into six groups on the basis of killing time (the 12th h, the first day, 3rd day, firs... Fifty six SD rats were randomly divided into the normal control group and the operation group. The operating group was subdivided into six groups on the basis of killing time (the 12th h, the first day, 3rd day, first week, 2nd week and 4th week) after vagotomy (VG). The pancreatic tissues were taken for HE and Feulgen staining. The DNA content of pancreatic exocrine cells was determined by a domestically fabricated computer image analyzing system. In the control group, on the first day or in the first week after VG, the pancreatic samples were taken for transmission electron microscopic examination. The DNA content of pancreatic exocrinal cells was decreased from 1 to 3 days after VG. The secretion was found to be in inhibitory state and One week later, it gradually restored. The results indicated that the proliferation and the function of the SD rat's pancreatic exocrinal cells were prohibited at initial stage after VG, which might be concerned, at least in part, with dominance and nutrition of vagus. 展开更多
关键词 VAGOTOMY pancreatic exocrinal cells DNA content
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Study of nennatolysosomes in mouse hepatocytes and pancreatic exocrine acinar cells
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作者 罗深秋 萧焕才 +2 位作者 朴英杰 Masahiro Sakai Kazuo Ogawa 《Journal of Medical Colleges of PLA(China)》 CAS 1992年第3期283-286,共4页
Acid phosphatase(ACPase)-positive nematolysosomes in mouse hepatocytes and thepancreatic exocrine acinar cells were studied with ultracytoehemical method.The nematolyso-somes were 2~8μm in length,0.1~0.3μm in diam... Acid phosphatase(ACPase)-positive nematolysosomes in mouse hepatocytes and thepancreatic exocrine acinar cells were studied with ultracytoehemical method.The nematolyso-somes were 2~8μm in length,0.1~0.3μm in diameter.Some branched and some did notbranch,and most of them winded through the organelles. 展开更多
关键词 HEPATOCYTES pancreatic exocrine acinar cells nematolysosomes ACID PHOSPHATASE
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小白鼠肝细胞和胰腺外分泌细胞的线状溶酶体研究
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作者 罗深秋 肖换才 +2 位作者 朴英杰 酒井真弘 小川和朗 《第一军医大学学报》 CSCD 1992年第2期97-100,共4页
用电镜酶细胞化学方法,显示了小白鼠肝细胞和胰腺外分泌细胞的线状溶酶体。该溶酶体呈酸性磷酸酶(ACPase)阳性反应,长条状,长度2~8μm,横径0.15~0.3μm,分支或不分支,多蜿蜒走行在细胞器之间。
关键词 肝细胞 溶酶体 电镜 细胞化学
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