The rice field frog Hoplobatrachus rugulosus is an important anuran species found in wetlands throughout Thailand. At present, the hematological parameters of wild populations are unknown. Therefore, hematological and...The rice field frog Hoplobatrachus rugulosus is an important anuran species found in wetlands throughout Thailand. At present, the hematological parameters of wild populations are unknown. Therefore, hematological and morphological characteristics of peripheral blood cells of wild-caught H. rugulosus were examined. Thirty-three adult frogs （17 male and 16 female frogs） were collected from a natural population in Nan Province, northem Thailand during the wet season of 2014. Blood samples were analyzed by packed cell volume （PCV） and blood cell counts from hemocytometer and Giemsa-stained blood smears. The mean PCV of male frogs （30.70% ±6.07%） was significantly higher than that of the female frogs （25.09%± 4.85%）. The mean number of lymphocytes and neutrophils also showed significant sex-related differences. Moreover, the morphometric analysis of blood cells revealed dimensions as follows： erythrocytes （17.96 ±1.44 μm length × 11.50 ± 1.09μm width）, immature erythrocytes （14.91 ± 2.20 μm diameter）, thrombocytes （13.93± 3.14 μm length × 7.05 ± 1.31 μm width）, lymphocytes （11.01 ±2.69μm diameter）, monocytes （12.04±2.40 μm diameter）, neutrophils （12.58 -±2.08μm diameter）, basophils （13.60 ± 2.17μm diameter） and eosinophils （12.33 ± 2.95 μm diameter）. Overall, the hematological parameters obtained in this study could be regarded as the first report and a crucial baseline data of wild H. rugulosus in Thailand that can be used for monitoring the health status of this anuran.展开更多
Numerical simulations are performed to examine the packing behavior of human red blood cells(RBCs). A combined ?nite-discrete element method(FDEM) is utilized, in which the RBCs are modeled as no-friction and no-adhes...Numerical simulations are performed to examine the packing behavior of human red blood cells(RBCs). A combined ?nite-discrete element method(FDEM) is utilized, in which the RBCs are modeled as no-friction and no-adhesion solid bodies. The packed volume and the void ratio of a large number of randomly packed RBCs are clari?ed,and the effects of the RBC shape, the mesh size, the cell number, and the container size are investigated. The results show that the packed human RBCs with normal shape have a void ratio of 28.45%, which is slightly higher than that of the ?at or thick cells used in this study. Such information is bene?cial to the further understanding on the geometric features of human RBCs and the research on RBC simulations.展开更多
Objective: To compare the efficacy of Oxytocin versus Methylergometrine in active management of third stage of labour in reducing risk of postpartum hemorrhage. Methods: This study was carried out by randomly assignin...Objective: To compare the efficacy of Oxytocin versus Methylergometrine in active management of third stage of labour in reducing risk of postpartum hemorrhage. Methods: This study was carried out by randomly assigning into two groups with 150 women in each group.?Group 1 included patients who received injection Oxytocin 10 IU intramuscular within one minute of the birth of the baby. Injection?Methylergometrine?(0.2 mg) was given intravenously at the delivery of anterior shoulder of the baby to women in Group 2. Outcome measures were the duration of third stage, blood loss, pre and post-delivery hematocrit, side effects and incidence of PPH. Statistical analysis was done using Chi square test, Fischers test, Mann Whitney test, and t test. p < 0.05 was considered significant. Results: Mean duration of third stage of labour, mean blood loss, post-delivery fall in hematocrit and need for additional uterotonics were significantly less in the Group 2. However side effects like nausea, vomiting and rise in blood pressure were significantly higher in women in Group 2 (p = 0.000). Conclusion: Methylergometrine is more effective than Oxytocin in reducing the duration of third stage of labour and blood loss but has significant side effects like nausea, vomiting and rise in blood pressure.展开更多
Several studies have shown that sickle cell disease (SCD) is made worse by infections;it was necessary to carry out this study to ascertain the occurrence of intestinal parasitic infections to the number of SCD patien...Several studies have shown that sickle cell disease (SCD) is made worse by infections;it was necessary to carry out this study to ascertain the occurrence of intestinal parasitic infections to the number of SCD patients infected compared to Non-SCD patients, the impact of the infections on Pack cell volume (PCV) of the SCD patients in Nigeria populace. A total of 140 stool samples were collected from both SCD patients and Non-SCD patients. 3 ml of venous blood, seventy samples in total were collected from the SCD patients only. The blood and stool samples were collected from September 2005 to November 2005;structured questionnaire was administered to each of the patient that gave consent to be part of the study alongside with questionnaire interview. The stool samples were analysed macroscopically and microscopically using saline, iodine and formal-ether concentration technique. The blood samples were analysed by micro-heamatocrit method. The findings showed that a total of six parasites were identified among the SCD patients and a total of thirteen parasites were identified among Non-SCD patients. Although the prevalence of intestinal parasitic infections was low among the research participates, the identified parasites fell into the 4 major categories of intestinal parasites, an indication that SCD patients can be susceptible to any of the intestinal worms/protozoa. Despite a low prevalence of intestinal parasites among the studied individual at the time of this study, it is however, necessary for regular laboratory investigations for intestinal worm/protozoa since they are still a public health problem.展开更多
基金supported by the Sponsorship of Graduate Student Research under the CU Academic Network in the region(CU-ANR-57-01),Chulalongkorn Universitythe 90th Anniversary of Chulalongkorn University Fund(Ratchadaphiseksomphot Endowment Fund)
文摘The rice field frog Hoplobatrachus rugulosus is an important anuran species found in wetlands throughout Thailand. At present, the hematological parameters of wild populations are unknown. Therefore, hematological and morphological characteristics of peripheral blood cells of wild-caught H. rugulosus were examined. Thirty-three adult frogs （17 male and 16 female frogs） were collected from a natural population in Nan Province, northem Thailand during the wet season of 2014. Blood samples were analyzed by packed cell volume （PCV） and blood cell counts from hemocytometer and Giemsa-stained blood smears. The mean PCV of male frogs （30.70% ±6.07%） was significantly higher than that of the female frogs （25.09%± 4.85%）. The mean number of lymphocytes and neutrophils also showed significant sex-related differences. Moreover, the morphometric analysis of blood cells revealed dimensions as follows： erythrocytes （17.96 ±1.44 μm length × 11.50 ± 1.09μm width）, immature erythrocytes （14.91 ± 2.20 μm diameter）, thrombocytes （13.93± 3.14 μm length × 7.05 ± 1.31 μm width）, lymphocytes （11.01 ±2.69μm diameter）, monocytes （12.04±2.40 μm diameter）, neutrophils （12.58 -±2.08μm diameter）, basophils （13.60 ± 2.17μm diameter） and eosinophils （12.33 ± 2.95 μm diameter）. Overall, the hematological parameters obtained in this study could be regarded as the first report and a crucial baseline data of wild H. rugulosus in Thailand that can be used for monitoring the health status of this anuran.
基金Project supported by the Engineering and Physical Sciences Research Council(EPSRC)Turbulence Consortium Grant(No.EP/G069581/1)the Marie Curie International Incoming Fellowship(No.PIIF-GA-253453)
文摘Numerical simulations are performed to examine the packing behavior of human red blood cells(RBCs). A combined ?nite-discrete element method(FDEM) is utilized, in which the RBCs are modeled as no-friction and no-adhesion solid bodies. The packed volume and the void ratio of a large number of randomly packed RBCs are clari?ed,and the effects of the RBC shape, the mesh size, the cell number, and the container size are investigated. The results show that the packed human RBCs with normal shape have a void ratio of 28.45%, which is slightly higher than that of the ?at or thick cells used in this study. Such information is bene?cial to the further understanding on the geometric features of human RBCs and the research on RBC simulations.
文摘Objective: To compare the efficacy of Oxytocin versus Methylergometrine in active management of third stage of labour in reducing risk of postpartum hemorrhage. Methods: This study was carried out by randomly assigning into two groups with 150 women in each group.?Group 1 included patients who received injection Oxytocin 10 IU intramuscular within one minute of the birth of the baby. Injection?Methylergometrine?(0.2 mg) was given intravenously at the delivery of anterior shoulder of the baby to women in Group 2. Outcome measures were the duration of third stage, blood loss, pre and post-delivery hematocrit, side effects and incidence of PPH. Statistical analysis was done using Chi square test, Fischers test, Mann Whitney test, and t test. p < 0.05 was considered significant. Results: Mean duration of third stage of labour, mean blood loss, post-delivery fall in hematocrit and need for additional uterotonics were significantly less in the Group 2. However side effects like nausea, vomiting and rise in blood pressure were significantly higher in women in Group 2 (p = 0.000). Conclusion: Methylergometrine is more effective than Oxytocin in reducing the duration of third stage of labour and blood loss but has significant side effects like nausea, vomiting and rise in blood pressure.
文摘Several studies have shown that sickle cell disease (SCD) is made worse by infections;it was necessary to carry out this study to ascertain the occurrence of intestinal parasitic infections to the number of SCD patients infected compared to Non-SCD patients, the impact of the infections on Pack cell volume (PCV) of the SCD patients in Nigeria populace. A total of 140 stool samples were collected from both SCD patients and Non-SCD patients. 3 ml of venous blood, seventy samples in total were collected from the SCD patients only. The blood and stool samples were collected from September 2005 to November 2005;structured questionnaire was administered to each of the patient that gave consent to be part of the study alongside with questionnaire interview. The stool samples were analysed macroscopically and microscopically using saline, iodine and formal-ether concentration technique. The blood samples were analysed by micro-heamatocrit method. The findings showed that a total of six parasites were identified among the SCD patients and a total of thirteen parasites were identified among Non-SCD patients. Although the prevalence of intestinal parasitic infections was low among the research participates, the identified parasites fell into the 4 major categories of intestinal parasites, an indication that SCD patients can be susceptible to any of the intestinal worms/protozoa. Despite a low prevalence of intestinal parasites among the studied individual at the time of this study, it is however, necessary for regular laboratory investigations for intestinal worm/protozoa since they are still a public health problem.
