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Articular cartilage and osteochondral tissue engineering techniques:Recent advances and challenges 被引量:21
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作者 Wenying Wei Honglian Dai 《Bioactive Materials》 SCIE 2021年第12期4830-4855,共26页
In spite of the considerable achievements in the field of regenerative medicine in the past several decades,osteochondral defect regeneration remains a challenging issue among diseases in the musculoskeletal system be... In spite of the considerable achievements in the field of regenerative medicine in the past several decades,osteochondral defect regeneration remains a challenging issue among diseases in the musculoskeletal system because of the spatial complexity of osteochondral units in composition,structure and functions.In order to repair the hierarchical tissue involving different layers of articular cartilage,cartilage-bone interface and subchondral bone,traditional clinical treatments including palliative and reparative methods have showed certain improvement in pain relief and defect filling.It is the development of tissue engineering that has provided more promising results in regenerating neo-tissues with comparable compositional,structural and functional characteristics to the native osteochondral tissues.Here in this review,some basic knowledge of the osteochondral units including the anatomical structure and composition,the defect classification and clinical treatments will be first introduced.Then we will highlight the recent progress in osteochondral tissue engineering from perspectives of scaffold design,cell encapsulation and signaling factor incorporation including bioreactor application.Clinical products for osteochondral defect repair will be analyzed and summarized later.Moreover,we will discuss the current obstacles and future directions to regenerate the damaged osteochondral tissues. 展开更多
关键词 osteochondral tissue engineering Cartilage tissue engineering Gradient scaffold Bioreactors
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Osteochondral tissue repair in osteoarthritic joints: clinical challenges and opportunities in tissue engineering 被引量:7
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作者 Maryam Tamaddon Ling Wang +1 位作者 Ziyu Liu Chaozong Liu 《Bio-Design and Manufacturing》 2018年第2期101-114,共14页
Osteoarthritis (OA), identified as one of the priorities for the Bone and Joint Decade, is one of the most prevalent joint diseases, which causes pain and disability of joints in the adult population. Secondary OA u... Osteoarthritis (OA), identified as one of the priorities for the Bone and Joint Decade, is one of the most prevalent joint diseases, which causes pain and disability of joints in the adult population. Secondary OA usually stems from repetitive overloading to the osteochondral (OC) unit, which could result in cartilage damage and changes in the subchondral bone, leading to mechanical instability of the joint and loss of joint function. Tissue engineering approaches have emerged for the repair of cartilage defects and damages to the subchondral bone in the early stages of OA and have shown potential in restoring the joint's function. In this approach, the use of three-dimensional scaffolds (with or without cells) provides support for tissue growth. Commercially available OC scaffolds have been studied in OA patients for repair and regeneration of OC defects. However, none of these scaffolds has shown satisfactory clinical results. This article reviews the OC tissue structure and the design, manufacturing and performance of current OC scaffolds in treatment of OA. The findings demonstrate the importance of biological and biomechanical fixations of OC scaffolds to the host tissue in achieving an improved cartilage fill and a hyaline-like tissue formation. Achieving a strong and stable subchondral bone support that helps the regeneration of overlying cartilage seems to be still a grand challenge for the early treatment of OA. 展开更多
关键词 osteochondral tissue engineering OSTEOARTHRITIS osteochondral scaffold Cartilage and subchondral bone Clinical scaffolds
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Integrated gradient tissue-engineered osteochondral scaffolds:Challenges,current efforts and future perspectives 被引量:2
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作者 Xiaolian Niu Ning Li +1 位作者 Zhipo Du Xiaoming Li 《Bioactive Materials》 SCIE CSCD 2023年第2期574-597,共24页
The osteochondral defect repair has been most extensively studied due to the rising demand for new therapies to diseases such as osteoarthritis.Tissue engineering has been proposed as a promising strategy to meet the ... The osteochondral defect repair has been most extensively studied due to the rising demand for new therapies to diseases such as osteoarthritis.Tissue engineering has been proposed as a promising strategy to meet the demand of simultaneous regeneration of both cartilage and subchondral bone by constructing integrated gradient tissue-engineered osteochondral scaffold(IGTEOS).This review brought forward the main challenges of establishing a satisfactory IGTEOS from the perspectives of the complexity of physiology and microenvironment of osteochondral tissue,and the limitations of obtaining the desired and required scaffold.Then,we comprehensively discussed and summarized the current tissue-engineered efforts to resolve the above challenges,including architecture strategies,fabrication techniques and in vitro/in vivo evaluation methods of the IGTEOS.Especially,we highlighted the advantages and limitations of various fabrication techniques of IGTEOS,and common cases of IGTEOS application.Finally,based on the above challenges and current research progress,we analyzed in details the future perspectives of tissue-engineered osteochondral construct,so as to achieve the perfect reconstruction of the cartilaginous and osseous layers of osteochondral tissue simultaneously.This comprehensive and instructive review could provide deep insights into our current understanding of IGTEOS. 展开更多
关键词 osteochondral tissue engineering Integrated gradient tissue-engineered osteochondral scaffold(IGTEOS) Tissue-engineered strategies Fabrication techniques Evaluation
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海藻酸盐支架修复关节软骨 被引量:3
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作者 李诚 郑国爽 +1 位作者 蒯贤东 于炜婷 《中国组织工程研究》 CAS 北大核心 2023年第7期1080-1088,共9页
背景:由于关节软骨的解剖、生理特点,其自我修复能力有限,故而如何修复大面积的软骨缺损(直径>4 mm)成为医学界备受瞩目的问题之一。伴随生物材料和组织工程学科的发展,通过支架技术尤其具有模拟细胞外基质微环境的水凝胶支架研究的... 背景:由于关节软骨的解剖、生理特点,其自我修复能力有限,故而如何修复大面积的软骨缺损(直径>4 mm)成为医学界备受瞩目的问题之一。伴随生物材料和组织工程学科的发展,通过支架技术尤其具有模拟细胞外基质微环境的水凝胶支架研究的深入发展,为软骨损伤修复提供了新的治疗思路。目的:就海藻酸盐的性质、海藻酸盐水凝胶支架的制备以及在软骨损伤修复中的研究进展进行综述。方法:利用计算机检索Web of Science、PubMed、万方和中国知网数据库,中文检索词为“海藻酸盐水凝胶、软骨组织工程或软骨、骨软骨、支架”,英文检索词为“alginate hydrogel,cartilage tissue engineering or chondro*,osteochondral,scaffold”,检索文献时间范围2016年1月至2020年12月。最终按入组标准筛选后纳入60篇文献进行综述分析。结果与结论:①海藻酸盐作为带负电荷的天然亲水性多糖,可通过修饰技术赋予传统海藻酸盐水凝胶更优良的机械性能、黏附性、生物降解性以及生物相容性等。②修饰后的海藻酸盐复合水凝胶支架利于维持种子细胞的正常形态、合成相应的细胞外基质,促进成软骨相关基因的表达,表现出优良的成软骨能力。③有报道证实海藻酸盐复合水凝胶支架可在动物体内形成与周边正常软骨相似的软骨组织,有效修复缺损部位。④因此,海藻酸盐复合水凝胶支架具备良好的促软骨修复能力,为软骨组织工程提供新的治疗思路,但该材料未来还需更完善的临床前试验数据的支撑,以推进其临床转化进程。 展开更多
关键词 海藻酸盐 软骨 骨软骨 支架 组织工程 修饰 水凝胶 综述
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Scaffolds based therapy for osteochondral lesions of the talus:A systematic review 被引量:4
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作者 Yoshiharu Shimozono Youichi Yasui +2 位作者 Andrew W Ross Wataru Miyamoto John G Kennedy 《World Journal of Orthopedics》 2017年第10期798-808,共11页
AIM To clarify the effectiveness of scaffold-based therapy for osteochondral lesions of the talus(OLT). METHODS A systematic search of MEDLINE and EMBASE databases was performed during August 2016 and updated in Janua... AIM To clarify the effectiveness of scaffold-based therapy for osteochondral lesions of the talus(OLT). METHODS A systematic search of MEDLINE and EMBASE databases was performed during August 2016 and updated in January 2017. Included studies were evaluated with regard to the level of evidence(LOE) and quality of evidence(QOE) using the Modified Coleman Methodology Score. Variable reporting outcome data, clinical outcomes, and the percentage of patients who returned to sport at previous level were also evaluated. RESULTS Twenty-eight studies for a total of 897 ankles were included; 96% were either LOE Ⅲ or Ⅳ. Studies were designated as either of poor or fair quality. There were 30 treatment groups reporting six different scaffold repair techniques: 13 matrix-induced autologous chondrocyte transplantation(MACT), nine bone marrow derived cell transplantation(BMDCT), four autologous matrixinduced chondrogeneis(AMIC), and four studies of other techniques. The categories of general demographics(93%) and patient-reported outcome data(85%) were well reported. Study design(73%), imaging data(73%), clinical variables(49%), and patient history(30%) were also included. The weighted mean American Orthopaedic Foot and Ankle Society(AOFAS) score at final follow-up was: 86.7 in MACT, 88.2 in BMDCT, and 82.3 in AMIC. Eight studies reported that a weighted mean of 68.3% ofpatients returned to a previous level of sport activity. CONCLUSION Scaffold-based therapy for OLT may produce favorable clinical outcomes, but low LOE, poor QOE, and variability of the data have confounded the effectiveness of this treatment. 展开更多
关键词 scaffold ANKLE Talar osteochondral LESION Systematic review CARTILAGE
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缓释万古霉素三维支架修复兔感染性骨软骨缺损
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作者 李兴屿 周杰 +5 位作者 李沙沙 张天喜 郭国宁 喻安永 邓江 叶鹏 《中国组织工程研究》 CAS 北大核心 2024年第22期3509-3516,共8页
背景:大量研究证实组织工程支架几乎可完全修复骨软骨缺损,但当骨软骨缺损合并感染时,即使前期经过彻底的清创,单纯骨软骨组织工程支架的修复效果往往不理想。目的:制备盐酸万古霉素缓释微球丝素蛋白/壳聚糖/纳米羟基磷灰石支架,观察其... 背景:大量研究证实组织工程支架几乎可完全修复骨软骨缺损,但当骨软骨缺损合并感染时,即使前期经过彻底的清创,单纯骨软骨组织工程支架的修复效果往往不理想。目的:制备盐酸万古霉素缓释微球丝素蛋白/壳聚糖/纳米羟基磷灰石支架,观察其对兔股骨远端感染性骨软骨缺损的修复效果。方法:①采用乳化溶剂挥发法制备盐酸万古霉素缓释微球;将不同质量(7.5,10,12.5 mg)的缓释微球分别与丝素蛋白-壳聚糖-纳米羟基磷灰石溶液混合,利用化学交联法制备盐酸万古霉素缓释微球丝素蛋白/壳聚糖/纳米羟基磷灰石支架,表征支架的孔隙率、吸水膨胀率、热水溶失率及体外药物缓释等。②将45只新西兰大白兔随机分为空白组、对照组、实验组,每组15只,均建立右后肢股骨远端骨软骨缺损并感染模型,空白组不作任何处理,对照组缺损处植入丝素蛋白-壳聚糖-纳米羟基磷灰石支架,实验组缺损处植入盐酸万古霉素缓释微球(10 mg)丝素蛋白/壳聚糖/纳米羟基磷灰石支架。术后1周,检测血液样本C-反应蛋白、白细胞水平;术后4,8,12周取术区组织,分别进行大体观察与病理学观察。结果与结论:①随着缓释微球含量的增加,支架的孔隙率降低,组间比较差异有显著性意义(P<0.05);3组支架的孔径大小、吸水膨胀率、热水溶失率比较差异均无显著性意义(P>0.05);3组支架体外均可持续释放盐酸万古霉素达30 d以上。②实验组兔血液样本C-反应蛋白、白细胞水平均低于空白组、对照组(P<0.05);实验组兔术后各时间点的大体软骨修复情况明显好于空白组、对照组;苏木精-伊红、Masson、阿利新蓝及Ⅱ型胶原免疫组化染色显示,实验组兔术后各时间点的骨软骨修复效果明显优于空白组、对照组。③结果表明,盐酸万古霉素缓释微球丝素蛋白/壳聚糖/纳米羟基磷灰石支架能有效促进开放性骨软骨� 展开更多
关键词 载药微球 万古霉素 骨软骨缺损 组织工程 丝素蛋白 壳聚糖 纳米羟基磷灰石 支架
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应用凝胶接种技术体外分层构建工程化骨软骨复合组织的初步研究 被引量:5
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作者 段小军 王富友 +4 位作者 杨柳 戴刚 谭洪波 陈光兴 郭林 《第三军医大学学报》 CAS CSCD 北大核心 2009年第20期1969-1972,共4页
目的应用组织工程原理,探索体外构建骨软骨复合组织的关键技术,为移植修复关节骨软骨组织缺损创造条件。方法采用组织分层构建策略,用兔成骨细胞和羟基磷灰石支架材料,采用凝胶接种技术,体外构建组织工程骨;用兔软骨细胞和聚乳酸/聚磷... 目的应用组织工程原理,探索体外构建骨软骨复合组织的关键技术,为移植修复关节骨软骨组织缺损创造条件。方法采用组织分层构建策略,用兔成骨细胞和羟基磷灰石支架材料,采用凝胶接种技术,体外构建组织工程骨;用兔软骨细胞和聚乳酸/聚磷酸钙纤维支架材料,采用凝胶接种技术,体外构建组织工程软骨;体外培养48h后,利用界面间凹凸进行压配,并结合蛋白胶粘贴形成工程化骨软骨复合组织;将构建组织移植到裸鼠皮下,以相同大小无细胞复合的支架材料为对照组,术后12周取材进行病理分析。结果采用凝胶接种技术,在体外可以初步构建组织工程骨和软骨,进而构建工程化骨软骨复合组织;工程化骨软骨复合组织移植到裸鼠皮下,术后12周实验组5例样本在成骨和成软骨区域观察到成骨和成软骨表现,但缺乏正常的钙化层界面结构,而对照组5例未观察到软骨组织形成。结论采用组织分层构建策略,采用简单的压配技术和新型凝胶接种技术可以在体外初步构建工程化骨软骨复合组织。 展开更多
关键词 组织工程 骨软骨 成骨细胞 软骨细胞 支架材料
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In vivo evaluation of additively manufacturedmulti-layered scaffold for the repair of large osteochondral defects 被引量:4
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作者 Maryam Tamaddon Gordon Blunn +10 位作者 Rongwei Tan Pan Yang Xiaodan Sun Shen-Mao Chen Jiajun Luo Ziyu Liu Ling Wang Dichen Li Ricardo Donate Mario Monzón Chaozong Liu 《Bio-Design and Manufacturing》 SCIE EI CAS CSCD 2022年第3期481-496,共16页
The repair of osteochondral defects is one of the major clinical challenges in orthopaedics.Well-established osteochondral tissue engineering methods have shown promising results for the early treatment of small defec... The repair of osteochondral defects is one of the major clinical challenges in orthopaedics.Well-established osteochondral tissue engineering methods have shown promising results for the early treatment of small defects.However,less success has been achieved for the regeneration of large defects,which is mainly due to the mechanical environment of the joint and the heterogeneous nature of the tissue.In this study,we developed a multi-layered osteochondral scaffold to match the heterogeneous nature of osteochondral tissue by harnessing additive manufacturing technologies and combining the established art laser sintering and material extrusion techniques.The developed scaffold is based on a titanium and polylactic acid matrix-reinforced collagen“sandwich”composite system.The microstructure and mechanical properties of the scaffold were examined,and its safety and efficacy in the repair of large osteochondral defects were tested in an ovine condyle model.The 12-week in vivo evaluation period revealed extensive and significantly higher bone in-growth in the multi-layered scaffold compared with the collagen–HAp scaffold,and the achieved stable mechanical fixation provided strong support to the healing of the overlying cartilage,as demonstrated by hyaline-like cartilage formation.The histological examination showed that the regenerated cartilage in the multi-layer scaffold group was superior to that formed in the control group.Chondrogenic genes such as aggrecan and collagen-II were upregulated in the scaffold and were higher than those in the control group.The findings showed the safety and efficacy of the cell-free“translation-ready”osteochondral scaffold,which has the potential to be used in a one-step surgical procedure for the treatment of large osteochondral defects. 展开更多
关键词 osteochondral scaffold Large animal Additive manufacturing Porous titanium
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Stable mechanical fixation in a bionic osteochondral scaffold considering bone growth 被引量:3
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作者 Jian Zhou Hao Huang +5 位作者 Li-Jing Wang Maryam Tamaddon Chao-Zong Liu Zi-Yu Liu Teng-Bo Yu Ying-Ze Zhang 《Rare Metals》 SCIE EI CAS CSCD 2022年第8期2711-2718,共8页
In the field of tissue engineering,there is significant subsidence of the porous design scaffold several months after implantation.To avoid stress shielding and stimulate bone and cartilage ingrowth,high scaffold poro... In the field of tissue engineering,there is significant subsidence of the porous design scaffold several months after implantation.To avoid stress shielding and stimulate bone and cartilage ingrowth,high scaffold porosity is needed to diminish the mechanical properties of the scaffold.The closer the mechanical properties of the scaffold are to those of surrounding tissues,the better biological properties it will get.Besides,adequate mechanical stability is needed as the scaffold needs to be well fixed in the target area and it will endure load after surgery.Evaluating the mechanical fixation of the scaffold at the initial stage and the long-term performance of a scaffold for in vivo study is hard,as no facility can be put into the target area for the friction test.This study investigated the mechanical stability of the biomimetic scaffold at the initial stage of implantation by finite element analysis(FEA).According to in vivo study,scaffold could not maintain its original position and would sink 1-2 mm in the target area.The simulation results suggested that mechanical loading is not the main reason for scaffold subsidence. 展开更多
关键词 osteochondral scaffold Mechanical fixation scaffold subsidence Bone ingrowth
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组织工程支架在骨软骨修复中的应用进展 被引量:4
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作者 马钢 肖云峰 +3 位作者 刘晓民 丁良甲 刘长路 高博 《医学综述》 2019年第3期453-458,共6页
因运动损伤、事故创伤所导致的骨软骨损伤逐渐增加,骨软骨损伤的修复也越来越受到重视。基于目前的研究,骨软骨组织工程支架在充分利用材料、信号分子的多种途径方面表现出巨大的潜能。支架设计需要考虑的基本要素是具有适当孔隙的双相... 因运动损伤、事故创伤所导致的骨软骨损伤逐渐增加,骨软骨损伤的修复也越来越受到重视。基于目前的研究,骨软骨组织工程支架在充分利用材料、信号分子的多种途径方面表现出巨大的潜能。支架设计需要考虑的基本要素是具有适当孔隙的双相结构和界面整合能力,组成物质的梯度分布和相适应的机械性能。支架的表面修饰技术可以改善细胞调节和信号分子递送。功能性支架可调控多信号转导分子递送的功能,被认为是有希望的治疗方法。 展开更多
关键词 骨软骨修复 界面组织 组织工程 支架设计 机械性能
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Osteochondral scaffolds for early treatment of cartilage defects in osteoarthritic joints:from bench to clinic 被引量:4
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作者 Maryam Tamaddon Helena Gilja +4 位作者 Ling Wang JMiguel Oliveira Xiaodan Sun Rongwei Tan Chaozong Liu 《Biomaterials Translational》 2020年第1期3-17,共15页
Osteoarthritis is a degenerative joint disease,typified by the loss in the quality of cartilage and bone at the interface of a synovial joint,resulting in pain,stiffness and reduced mobility.The current surgical treat... Osteoarthritis is a degenerative joint disease,typified by the loss in the quality of cartilage and bone at the interface of a synovial joint,resulting in pain,stiffness and reduced mobility.The current surgical treatment for advanced stages of the disease is joint replacement,where the non-surgical therapeutic options or less invasive surgical treatments are no longer effective.These are major surgical procedures which have a substantial impact on patients’quality of life and lifetime risk of requiring revision surgery.Treatments using regenerative methods such as tissue engineering methods have been established and are promising for the early treatment of cartilage degeneration in osteoarthritis joints.In this approach,3-dimensional scaffolds(with or without cells)are employed to provide support for tissue growth.However,none of the currently available tissue engineering and regenerative medicine products promotes satisfactory durable regeneration of large cartilage defects.Herein,we discuss the current regenerative treatment options for cartilage and osteochondral(cartilage and underlying subchondral bone)defects in the articulating joints.We further identify the main hurdles in osteochondral scaffold development for achieving satisfactory and durable regeneration of osteochondral tissues.The evolution of the osteochondral scaffolds–from monophasic to multiphasic constructs–is overviewed and the osteochondral scaffolds that have progressed to clinical trials are examined with respect to their clinical performances and their potential impact on the clinical practices.Development of an osteochondral scaffold which bridges the gap between small defect treatment and joint replacement is still a grand challenge.Such scaffold could be used for early treatment of cartilage and osteochondral defects at early stage of osteoarthritis and could either negate or delay the need for joint replacements. 展开更多
关键词 cartilage injury OSTEOARTHRITIS osteochondral scaffold regenerative medicine subchondral defect tissue engineering
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骨软骨一体化支架的制备及力学性能研究
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作者 冯鹿明 罗洋 +3 位作者 高丽兰 谭沿松 刘钢 张春秋 《天津理工大学学报》 2023年第5期64-70,共7页
按一定比例混合Ⅱ型胶原、丝素蛋白与透明质酸3种天然材料,运用冷冻干燥技术与天然软骨下骨交联,制备出同时具有软骨层和软骨下骨层的骨软骨一体化支架。采用扫描电子显微镜(scanning electron microscope,SEM)及非接触数字技术研究分... 按一定比例混合Ⅱ型胶原、丝素蛋白与透明质酸3种天然材料,运用冷冻干燥技术与天然软骨下骨交联,制备出同时具有软骨层和软骨下骨层的骨软骨一体化支架。采用扫描电子显微镜(scanning electron microscope,SEM)及非接触数字技术研究分析支架的表观与微观结构,通过压缩试验及循环载荷下的平均应变试验,对支架的力学性能进行检测。结果表明:制备出的骨软骨一体化支架软骨层呈多孔孔隙结构,通透性较好,且具有明显的率相关性;随着压缩速率的增加,支架的弹性模量增加;在循环压缩载荷作用下,支架显示了应力-应变滞环曲线,随着加载圈数的增加,支架表现出明显的平均应变行为,对骨软骨缺损修复具有重要的临床价值。 展开更多
关键词 骨软骨缺损 一体化支架 天然材料 微观结构 力学性能
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组织工程骨软骨复合组织构建研究进展 被引量:1
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作者 赵家宁 徐宝山 杨强 《中国矫形外科杂志》 CAS CSCD 北大核心 2014年第23期2156-2160,共5页
本文综述了近几年骨软骨组织工程研究进展。查阅近几年有关骨软骨组织工程的文献,于支架材料、信号因子、种子细胞及制备技术等方面加以总结分析。骨软骨组织工程根据仿生学理论与方法完成组织替代物的制备并对损伤或有缺陷组织进行修复... 本文综述了近几年骨软骨组织工程研究进展。查阅近几年有关骨软骨组织工程的文献,于支架材料、信号因子、种子细胞及制备技术等方面加以总结分析。骨软骨组织工程根据仿生学理论与方法完成组织替代物的制备并对损伤或有缺陷组织进行修复,可有效恢复受损骨软骨组织的结构功能与力学特性,是一种具有长远意义的综合交叉学科。目前,骨软骨组织工程策略针对骨软骨损伤取得了一定的成果,但仍需进一步发展,最终为骨软骨损伤提供一种成熟的临床治疗方法。 展开更多
关键词 骨软骨组织工程 骨软骨损伤 骨软骨支架
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仿生多材料复合增强骨软骨支架的制造及性能研究 被引量:3
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作者 庄佩 连芩 +4 位作者 李涤尘 贺健康 边卫国 王臻 靳忠民 《机械工程学报》 EI CAS CSCD 北大核心 2014年第21期133-139,共7页
针对关节面上大面积骨软骨缺损修复过程中软骨形态恢复和力学环境恢复困难的问题,设计并制造一种新型聚乙二醇(Polyethylene glycol, PEG)/聚乳酸(Polylactide, PLA)/β-磷酸三钙(β-Tricalcium phosphate,β-TCP)仿生多材料复... 针对关节面上大面积骨软骨缺损修复过程中软骨形态恢复和力学环境恢复困难的问题,设计并制造一种新型聚乙二醇(Polyethylene glycol, PEG)/聚乳酸(Polylactide, PLA)/β-磷酸三钙(β-Tricalcium phosphate,β-TCP)仿生多材料复合增强骨软骨支架。基于CT扫描数据重建的羊膝关节模型上进行仿生多材料骨软骨支架的结构设计,包括多孔定制结构和固定桩及仿生结构;以光固化成形技术与真空灌注工艺相结合制造了的多材料复合增强骨软骨支架,确定灌注温度220℃,真空度–0.08~–0.10 Pa。形貌观测表明真空灌注法能使PLA完全充满整个次级管道,力学试验发现复合材料支架的压缩强度(21.25 MPa ±1.15 MPa)是单管道多孔生物陶瓷支架(9.76 MPa±0.64 MPa)的2.17倍, PLA固定桩的剪切强度(16.24 MPa±1.85 MPa)是陶瓷固定桩(0.87 MPa±0.14 MPa)的18.7倍。因此,复合PLA的骨软骨支架具有显著的力学增强和固定能力,有望为大面积骨软骨缺损的修复提供新的治疗手段。 展开更多
关键词 三维打印 仿生设计 骨软骨支架 聚乳酸 Β-磷酸三钙
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新型骨-软骨一体化修复支架材料的制备 被引量:2
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作者 任力 季培红 +1 位作者 黄志芳 王迎军 《复合材料学报》 EI CAS CSCD 北大核心 2011年第1期99-103,共5页
利用可降解聚合物微球的相互粘结制备了一种新型的组织工程支架材料,可用于软骨和软骨下骨损伤的修复。采用光学显微镜、扫描电镜对支架的表面形貌、内部结构进行了表征,同时研究了支架材料的力学性能,此外还研究了微球的粒径对支架材... 利用可降解聚合物微球的相互粘结制备了一种新型的组织工程支架材料,可用于软骨和软骨下骨损伤的修复。采用光学显微镜、扫描电镜对支架的表面形貌、内部结构进行了表征,同时研究了支架材料的力学性能,此外还研究了微球的粒径对支架材料孔隙率的影响。结果显示,该材料在结构上分为乳酸-羟基乙酸共聚物(PLGA)层和PLGA/生物活性玻璃(BG)层;材料的孔隙三维连通、分布均匀;采用粒径为150~200μm微球所制备的支架孔隙率为(53.37±4.39)%,在10%的应变下材料压缩强度便已达到了0.9 MPa,显示了较强的力学性能;随着微球粒径的变小,材料孔隙率逐渐增大。这种微球支架在骨-软骨组织缺损修复方面有着很大的研究价值和应用价值。 展开更多
关键词 软骨 微球 支架 PLGA 生物活性玻璃
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Current researches on design and manufacture of biopolymer-based osteochondral biomimetic scaffolds 被引量:2
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作者 Yanen Wang Ying Guo +3 位作者 Qinghua Wei Xinpei Li Kang Ji Kun Zhang 《Bio-Design and Manufacturing》 SCIE EI CSCD 2021年第3期541-567,共27页
Currently,osteochondral(OC)tissue engineering has become a potential treatment strategy in repairing chondral lesions and early osteoarthritis due to the limited self-healing ability of cartilage.However,it is still c... Currently,osteochondral(OC)tissue engineering has become a potential treatment strategy in repairing chondral lesions and early osteoarthritis due to the limited self-healing ability of cartilage.However,it is still challenging to ensure the integrity,physiological function and regeneration ability of stratified OC scaffolds in clinical application.Biomimetic OC scaffolds are attractive to overcome the above problems because of their similar biological and mechanical properties with native OC tissue.As a consequence,the researches on biomimetic design to achieve the tissue function of each layer,and additive manufacture(AM)to accomplish composition switch and ultrastructure of personalized OC scaffolds have made a remarkable progress.In this review,the design methods of biomaterial and structure as well as computer-aided design,and performance prediction of biopolymer-based OC scaffolds are presented;then,the characteristics and limitations of AM technologies and the integrated manufacture schemes in OC tissue engineering are summarized;finally,the novel biomaterials and techniques and the inevitable trends of multifunctional bio-manufacturing system are discussed for further optimizing production of tissue engineering OC scaffolds. 展开更多
关键词 osteochondral tissue Biomimetic scaffold Additive manufacture Integrated manufacture
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丝素蛋白壳聚糖和纳米羟基磷灰石制备骨软骨梯度孔径支架初探 被引量:2
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作者 肖红利 黄文良 +3 位作者 熊坤 李茂伦 陈禺 邓江 《中华老年医学杂志》 CAS CSCD 北大核心 2018年第7期816-820,共5页
目的探索丝素蛋白(SF)、壳聚糖(CS)和纳米羟基磷厌石(nHA)制备骨、软骨梯度孔径支架方法及可行性。方法制备浓度为2%的SF和CS溶液及nHA悬液,三者等比例混合,采用离心-冷冻干燥法及化学交联法制备SF/CS/nHA骨、软骨梯度孔径支... 目的探索丝素蛋白(SF)、壳聚糖(CS)和纳米羟基磷厌石(nHA)制备骨、软骨梯度孔径支架方法及可行性。方法制备浓度为2%的SF和CS溶液及nHA悬液,三者等比例混合,采用离心-冷冻干燥法及化学交联法制备SF/CS/nHA骨、软骨梯度孔径支架,检测支架孔隙率、热水溶失率、吸水膨胀率及力学性能,绘制溶失曲线及应力-应变曲线,采用扫描电镜(SEM)观察支架内部结构及形态并测量支架孔 径大小。结果SF/CS/nHA骨、软骨梯度孔径支架孔隙率为(91.30±3.35)%,5周后热水溶失率为(16.57±3.18)%,吸水膨胀率为(3218.53±84.37)%,力学检测结果显示支架抗压性能良好。SEM显示支架内部孔隙呈蜂窝状、相互交通,孔隙分布从上到下逐渐密集,孔径大小从上至下逐渐减小,分别为(141.1]±11.85)μm、(119.94±9.05)μm、(93.10±14.98)μm、(79.95±8.65)μm,各层互相比较差异有统计学意义(F=22.973,P=0.000)。支架细胞毒性检测结果显示各个时间段浸提液组A值与阴性对照组相比差异均无统计学意义(t24h=0.520,P=0.610;t48h=0.665,P=0.515;t72h=0.439,P=0.666),细胞卡H对增殖率(RGR)值均大于100%。结论利用离心冷冻干燥法及化学交联法可初步制备SF/CS/nHA骨、软骨梯度孔径支架。支架呈三维立体结构、渐进的梯度式孔径、高孔隙率、极强的吸水性、适官的降解速率及良好的抗压性能,支架无明显细胞毒性,细胞相容性良好,基本符合骨组织工程材料的要求。 展开更多
关键词 丝素蛋白类 壳聚糖 纳米羟基磷灰石 骨软骨支架
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含天然钙化层的骨软骨材料修复猪膝关节软骨缺损
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作者 黄杨 张颖 +3 位作者 王富友 曾伟南 刘俊利 杨柳 《第三军医大学学报》 CAS CSCD 北大核心 2018年第4期307-315,共9页
目的构建含和不含天然钙化层的修复材料,比较两者在猪膝关节软骨缺损修复中的效果,探讨钙化层在骨软骨组织工程中的重要性。方法选取普通实验猪膝关节骨,软骨通过Ⅱ型胶原水凝胶冻干技术和天然骨软骨脱细胞技术构建含有钙化层和无钙化... 目的构建含和不含天然钙化层的修复材料,比较两者在猪膝关节软骨缺损修复中的效果,探讨钙化层在骨软骨组织工程中的重要性。方法选取普通实验猪膝关节骨,软骨通过Ⅱ型胶原水凝胶冻干技术和天然骨软骨脱细胞技术构建含有钙化层和无钙化层的骨软骨支架,以贵州小香猪自体骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)为种子细胞,构建移植修复材料。30只小香猪按随机数字表法分为空白对照组、无钙化层组、含钙化层组(n=10)。双膝关节滑车骨软骨缺损造模,直径8 mm,深至软骨下骨,植入对应的修复材料。分别于12、24周取材,采用大体、体式显微镜观察,以及MRI检测缺损填充情况,HE染色、番红O-固绿染色,以及Ⅰ、Ⅱ型胶原蛋白免疫组化染色观察新生修复物的组织类别,O’Driscoll组织学评分评价修复效果。结果各组术后24周修复效果较12周均有改善。大体和体式显微镜观察,24周空白对照组新生组织部分填充缺损,为纤维组织,软骨表面凹陷深大;无钙化层组移植物填充缺损,软骨表面凹陷;含钙化层组缺损填充较好,表面微凹陷,三层结构可见,与宿主组织整合佳。组织学观察提示空白对照组为纤维组织填充修复;无钙化层组为骨和纤维软骨修复,三层结构不明显;含钙化层组钙化层结构清晰,移植物与宿主整合,软骨表面微凹陷,透明软骨修复。O’Driscoll组织学评分,12、24周空白对照组分别为(3.80±0.83)、(5.50±0.52)分,无钙化层组分别为(10.30±0.63)、(14.20±0.68)分,含钙化层组分别为(15.10±0.58)、(18.80±0.87)分,各组评分差异均有统计学意义(P<0.01),含钙化层组评分最高。结论含有天然钙化层的骨软骨支架,在猪骨软骨缺损修复中取得了很好的修复效果,明显优于无钙化层支架和空白对照,是今后软骨组织工程支架设计的重要参考。 展开更多
关键词 组织工程 钙化层 软骨缺损 骨软骨支架 自体 贵州小香猪
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3D打印技术构建含界层结构关节骨软骨支架的现状与展望
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作者 杨柳 黄杨 《陆军军医大学学报》 CAS CSCD 北大核心 2022年第15期1493-1500,共8页
关节骨软骨缺损在临床上十分常见,组织工程技术方法可为这一病损提供具有广阔前景的再生治疗方案。关节骨软骨单元由透明软骨、钙化软骨层、软骨下骨构成,其中钙化软骨层作为关节骨与软骨的界层结构,在应力传导和微环境调控方面均具有... 关节骨软骨缺损在临床上十分常见,组织工程技术方法可为这一病损提供具有广阔前景的再生治疗方案。关节骨软骨单元由透明软骨、钙化软骨层、软骨下骨构成,其中钙化软骨层作为关节骨与软骨的界层结构,在应力传导和微环境调控方面均具有重要作用;含有界层结构复合支架用于再生修复这类缺损是未来关节骨软骨复合组织工程的发展方向。3D打印制造技术具有快速、精准、个性化定制的优势和特点,能够满足含界层结构的关节骨软骨支架不规则几何外形与多层结构、成分差异化构建的需求。本文概述了关节骨软骨单元的形态结构、生化组分及生理功能,评述了关节骨软骨复合组织工程支架中构建其界层结构的必要性,以及利用材料挤出、熔融电写、静电纺丝、光固化和数字光处理等3D打印技术构建含界层结构骨软骨支架的方案。未来需要深入骨软骨界层结构的基础研究,并且积极开发和拓展3D打印技术在含有界层结构的关节骨软骨支架构建方面的应用,从而更好地实现骨软骨支架的功能和结构的仿生,最终提升各类伤病所致关节骨软骨缺损的再生治疗水平。 展开更多
关键词 3D打印 组织工程 骨软骨支架 钙化软骨层
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脂肪间充干细胞复合骨软骨一体化支架修复兔骨软骨缺损的实验研究
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作者 杰永生 郑蕊 +4 位作者 陈磊 靳少锋 孙磊 舒雄 綦惠 《中国医药生物技术》 2020年第5期469-474,共6页
目的探讨脂肪间充质干细胞复合骨软骨一体化支架修复兔骨软骨缺损的效果及其临床应用的可能性和优势。方法成年新西兰大白兔构建骨软骨缺损动物模型。A组为空白对照组,仅做缺损手术;B组为骨软骨一体化支架组,植入不含细胞的支架;C组为... 目的探讨脂肪间充质干细胞复合骨软骨一体化支架修复兔骨软骨缺损的效果及其临床应用的可能性和优势。方法成年新西兰大白兔构建骨软骨缺损动物模型。A组为空白对照组,仅做缺损手术;B组为骨软骨一体化支架组,植入不含细胞的支架;C组为负载脂肪间充质干细胞的骨软骨一体化支架组。术后6、12周取材,进行大体观察,根据国际软骨修复协会(ICRS)组织学评分标准评分,HE染色和番红O-固绿染色进行组织学评估。结果术后6周,一体化支架组与ADSCs复合一体化支架组的ICRS评分显著高于空白对照组(P<0.05);术后12周,一体化支架组与ADSCs复合支架组的ICRS评分仍显著高于空白对照组(P<0.05),且ADSCs复合一体化支架组的评分显著高于一体化支架组(P<0.05)。HE染色、甲苯胺蓝与番红O-固绿染色表明,一体化支架组和ADSCs复合一体化支架组的修复明显优于空白对照组,且ADSCs复合一体化支架组的修复较一体化支架组更优。结论骨软骨一体化支架能够促进兔膝关节骨软骨缺损的修复,但修复效果有限。ADSCs能够明显提高支架的作用,ADSCs复合一体化支架是此次动物实验中最佳的骨软骨修复方式。 展开更多
关键词 骨软骨 一体化支架 脂肪间充质干细胞 软骨分化
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