为明确杨树对氮沉降和增水的响应机制,采用2因素(氮和水添加)随机区组设计,设置4个施氮水平(0、5、10和15 g N·m^(-2)·a^(-1))以及3个水分水平(自然降水、自然降水增加50%和增加100%),测定杨树幼苗不同器官(叶、枝、茎、粗根...为明确杨树对氮沉降和增水的响应机制,采用2因素(氮和水添加)随机区组设计,设置4个施氮水平(0、5、10和15 g N·m^(-2)·a^(-1))以及3个水分水平(自然降水、自然降水增加50%和增加100%),测定杨树幼苗不同器官(叶、枝、茎、粗根和细根)中C、N、P含量,分析各器官元素间的关系。结果表明:随施氮量增加,粗根和细根N含量和N∶P及细根P含量增加,粗根和细根C∶N及细根C∶P下降。随降水增加,叶和细根C含量下降,茎和粗根C含量保持稳定,粗根N∶P上升。施氮与增水对杨树幼苗化学计量具有交互作用。自然降水下,随施氮量增加,枝、茎、粗根和细根C含量、叶、枝和茎N含量和P含量变化不显著;增水50%处理下,随施氮量增加,叶、枝和细根C含量、各器官N含量及叶、茎和粗根P含量增加;增水100%处理下,随施氮量增加,叶、茎和粗根C和P含量变化不显著。相同元素(C、N、P)在杨树幼苗不同器官中具有较高的正相关关系,N与P含量在叶和细根中呈显著正相关。因此,自然降水下,施氮只影响杨树幼苗地下部分N、P分配;降水增加50%下,施氮促进了各器官C、N、P分配;而降水增加100%减弱了氮添加的影响。展开更多
BACKGROUND Acute appendicitis in a solid organ transplant recipient is a rare occurrence, and experience remains limited. Appendicitis in uterine transplant recipients has never been reported. Immunocompromised patien...BACKGROUND Acute appendicitis in a solid organ transplant recipient is a rare occurrence, and experience remains limited. Appendicitis in uterine transplant recipients has never been reported. Immunocompromised patients with acute abdomen often present late and with attenuated symptoms. The differential diagnosis in a transplanted patient is broad and challenging due to possible existing complications associated with the graft, effects of immunosuppression, and altered anatomical relations.CASE SUMMARY A 26-year-old woman suffering from absolute uterine factor infertility received a uterus transplant. In the post-transplant period, she suffered from leukopenia and recurrent acute cellular rejection. Her compliance was suboptimal. She travelled to an exotic destination despite the physician's recommendation not to do so. Following her vacation, she presented with abdominal discomfort, nausea and diarrhoea. There was no sign of acute abdomen;the abdominal ultrasound was negative on day 0. Clostridium difficile colitis was verified and treated with perorally administered vancomycin. On day 4, the discomfort changed to pain;the ultrasound scan revealed a finding suggestive of appendicitis. Surgical exploration revealed perforated appendicitis, and appendectomy was performed.From a surgical point of view, the patient's follow-up was uneventful. The patient underwent a successful embryo transfer 6 months after the appendectomy. The patient gave birth to a healthy boy at the 35 th week of gestation.CONCLUSION A high index of suspicion of an atypical course and symptomatology of acute abdomen should be maintained in immunosuppressed patients.展开更多
A rice CaMBP gene, OsCaMBP （AB363406）, was isolated from a chilling treated rice using the fluorescent differential display （FDD） screening method. Its cDNA sequence （2094 bp） contains an opening reading frame ...A rice CaMBP gene, OsCaMBP （AB363406）, was isolated from a chilling treated rice using the fluorescent differential display （FDD） screening method. Its cDNA sequence （2094 bp） contains an opening reading frame （ORF） encoding a 569 amino acids protein （63.2 kD）. OsCaMBP has the typical structural features of the CaMBP family, including the conserved IQ calmodulin-binding motif at the N-terminus. Homology analysis revealed 38.25%-47.28% identities of OsCaMBP with other CaMBPs in plants. RT-PCR analysis showed that the expression of OsCaMBP was remarkably inducible under the chilling （8℃） and heat-shock （42℃） treatments. OsCaMBP was undetectable under the normal conditions, and induced under the chilling treatment for 1 h, as well as the heat-shock treatment for 15 min, suggesting that the gene plays important roles in the signaling pathway in rice under both chilling and heat-shock stresses.展开更多
文摘为明确杨树对氮沉降和增水的响应机制,采用2因素(氮和水添加)随机区组设计,设置4个施氮水平(0、5、10和15 g N·m^(-2)·a^(-1))以及3个水分水平(自然降水、自然降水增加50%和增加100%),测定杨树幼苗不同器官(叶、枝、茎、粗根和细根)中C、N、P含量,分析各器官元素间的关系。结果表明:随施氮量增加,粗根和细根N含量和N∶P及细根P含量增加,粗根和细根C∶N及细根C∶P下降。随降水增加,叶和细根C含量下降,茎和粗根C含量保持稳定,粗根N∶P上升。施氮与增水对杨树幼苗化学计量具有交互作用。自然降水下,随施氮量增加,枝、茎、粗根和细根C含量、叶、枝和茎N含量和P含量变化不显著;增水50%处理下,随施氮量增加,叶、枝和细根C含量、各器官N含量及叶、茎和粗根P含量增加;增水100%处理下,随施氮量增加,叶、茎和粗根C和P含量变化不显著。相同元素(C、N、P)在杨树幼苗不同器官中具有较高的正相关关系,N与P含量在叶和细根中呈显著正相关。因此,自然降水下,施氮只影响杨树幼苗地下部分N、P分配;降水增加50%下,施氮促进了各器官C、N、P分配;而降水增加100%减弱了氮添加的影响。
文摘BACKGROUND Acute appendicitis in a solid organ transplant recipient is a rare occurrence, and experience remains limited. Appendicitis in uterine transplant recipients has never been reported. Immunocompromised patients with acute abdomen often present late and with attenuated symptoms. The differential diagnosis in a transplanted patient is broad and challenging due to possible existing complications associated with the graft, effects of immunosuppression, and altered anatomical relations.CASE SUMMARY A 26-year-old woman suffering from absolute uterine factor infertility received a uterus transplant. In the post-transplant period, she suffered from leukopenia and recurrent acute cellular rejection. Her compliance was suboptimal. She travelled to an exotic destination despite the physician's recommendation not to do so. Following her vacation, she presented with abdominal discomfort, nausea and diarrhoea. There was no sign of acute abdomen;the abdominal ultrasound was negative on day 0. Clostridium difficile colitis was verified and treated with perorally administered vancomycin. On day 4, the discomfort changed to pain;the ultrasound scan revealed a finding suggestive of appendicitis. Surgical exploration revealed perforated appendicitis, and appendectomy was performed.From a surgical point of view, the patient's follow-up was uneventful. The patient underwent a successful embryo transfer 6 months after the appendectomy. The patient gave birth to a healthy boy at the 35 th week of gestation.CONCLUSION A high index of suspicion of an atypical course and symptomatology of acute abdomen should be maintained in immunosuppressed patients.
基金supported by the Program for Changjiang Scholars and Innovative Research Team in University of China (Grant No. IRT0453)the National Science & Technology Pillar Program of China in the Eleventh Five-Year Plan Period (Grant No. 2007BAD81B00).
文摘A rice CaMBP gene, OsCaMBP （AB363406）, was isolated from a chilling treated rice using the fluorescent differential display （FDD） screening method. Its cDNA sequence （2094 bp） contains an opening reading frame （ORF） encoding a 569 amino acids protein （63.2 kD）. OsCaMBP has the typical structural features of the CaMBP family, including the conserved IQ calmodulin-binding motif at the N-terminus. Homology analysis revealed 38.25%-47.28% identities of OsCaMBP with other CaMBPs in plants. RT-PCR analysis showed that the expression of OsCaMBP was remarkably inducible under the chilling （8℃） and heat-shock （42℃） treatments. OsCaMBP was undetectable under the normal conditions, and induced under the chilling treatment for 1 h, as well as the heat-shock treatment for 15 min, suggesting that the gene plays important roles in the signaling pathway in rice under both chilling and heat-shock stresses.
