Bacterial endotoxins are a major concern in periodontal health and diseases owing to their structure and biological activity.With up-to-date knowledge of endotoxins and the recent findings about the influence of endot...Bacterial endotoxins are a major concern in periodontal health and diseases owing to their structure and biological activity.With up-to-date knowledge of endotoxins and the recent findings about the influence of endotoxins in dental health,their probable mode of pathogenesis,and standard detection methods,this review analyzes the potential efficacy and benefits of probiotics in combination with conventional and contemporary treatment measures.In the oral cavity,Gram-negative bacteria are documented to predominate in the pulpal lesions with radiolucent areas and in the root canal with pulp necrosis,where they pose an absolute threat by promoting a series of inflammatory reactions.Endotoxin,a constituent of Gram-negative bacteria establishes a nexus between cytokine stimulation and proinflammatory reactions,therefore plays a critical role in decaying dental pulp and modulating periodontal diseases.Currently,the treatment regimen involves several biochemical preparations.In addition,probiotics have been reported to control endotoxin in gingivitis and contribute to the overall improvement of dental health.A potential benefit of a combination of probiotics as a complementary treatment along with the conventional treatment warrant more empirical evidence to elucidate its role and mechanism in resolving the clinical manifestations associated with endotoxins in the periodontal region.展开更多
Due to its safety,convenience,low cost and good compliance,oral administration attracts lots of attention.However,the efficacy of many oral drugs is limited to their unsatisfactory bioavailability in the gastrointesti...Due to its safety,convenience,low cost and good compliance,oral administration attracts lots of attention.However,the efficacy of many oral drugs is limited to their unsatisfactory bioavailability in the gastrointestinal tract.One of the critical and most overlooked factors is the symbiotic gut microbiota that can modulate the bioavailability of oral drugs by participating in the biotransformation of oral drugs,influencing the drug transport process and altering some gastrointestinal properties.In this review,we summarized the existing research investigating the possible relationship between the gut microbiota and the bioavailability of oral drugs,which may provide great ideas and useful instructions for the design of novel drug delivery systems or the achievement of personalized medicine.展开更多
The vaginal microbiota is less complex than the gut microbiota,and the colonization of Lactobacillus in the female vagina is considered to be critical for reproductive health.Oral probiotics have been suggested as pro...The vaginal microbiota is less complex than the gut microbiota,and the colonization of Lactobacillus in the female vagina is considered to be critical for reproductive health.Oral probiotics have been suggested as promising means to modulate vaginal homeostasis in the general population.In this study,60 Chinese women were followed for over a year before,during,and after treatment with the probiotics Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14.Shotgun metagenomic data of 1334 samples from multiple body sites did not support a colonization route of the probiotics from the oral cavity to the intestinal tract and then to the vagina.Our analyses enable the classification of the cervicovaginal microbiome into a stable state and a state of dysbiosis.The microbiome in the stable group steadily maintained a relatively high abundance of Lactobacilli over one year,which was not affected by probiotic intake,whereas in the dysbiosis group,the microbiota was more diverse and changed markedly over time.Data from a subset of the dysbiosis group suggests this subgroup possibly benefited from supplementation with the probiotics,indicating that probiotics supplementation can be prescribed for women in a subclinical microbiome setting of dysbiosis,providing opportunities for targeted and personalized microbiome reconstitution.展开更多
Background: Periodontal infections are the most common disease of cats and dogs. About 70% - 80% of them suffer from these infections during their lifetime. These infections occur in two forms, gingivitis and periodon...Background: Periodontal infections are the most common disease of cats and dogs. About 70% - 80% of them suffer from these infections during their lifetime. These infections occur in two forms, gingivitis and periodontitis. Gingivitis is a reversible inflammation of the gingiva which can be cured. As with humans, the harmful bacteria can cause infections also in organs such as the heart, liver and kidneys. Besides veterinary treatment, also the owner’s everyday care is essential. Objective: The aim of the study was to develop and clinically test easy home care probiotic product for cats’ and dogs’ oral health. Methods: Nine cats and thirteen dogs were given a combination of selected bacteria Streptococcus thermophilus SP4, Lactobacillus plantarum 14D and Lactobacillus rhamnosus SP1 (Petguide powder) in powder form daily for four weeks on top of the dry feed. Microbiological samples were taken before and after the intervention from each dog and cat. The samples were analyzed with flow cytometric analysis using Porphyromonas gingivalis and Fusobacterium sp. as indicators for oral health. Results: The home care treatment with these specific, live probiotic bacteria was shown to prevent the growth of infectious bacteria within four weeks. Petguide probiotic powder reduced the relative abundance of infectious microbes significantly (p = 0.005) in cats and dogs. Conclusion: The results suggest that the probiotic product with three live strains of probiotic bacteria prevents the growth of infectious bacterial species that are known to cause infections in pets’ mouths. Therefore, this probiotic combination could be used to improve the oral health of pets and increase their quality of life.展开更多
A massive depletion of CD4+T lymphocytes has been described in early and acute human immunodeficiency virus(HIV)infection,leading to an imbalance between the human microbiome and immune responses.In recent years,a gro...A massive depletion of CD4+T lymphocytes has been described in early and acute human immunodeficiency virus(HIV)infection,leading to an imbalance between the human microbiome and immune responses.In recent years,a growing interest in the alterations in gut microbiota in HIV infection has led to many studies;however,only few studies have been conducted to explore the importance of oral microbiome in HIV-infected individuals.Evidence has indicated the dysbiosis of oral microbiota in people living with HIV(PLWH).Potential mechanisms might be related to the immunodeficiency in the oral cavity of HIV-infected individuals,including changes in secretory components such as reduced levels of enzymes and proteins in saliva and altered cellular components involved in the reduction and dysfunction of innate and adaptive immune cells.As a result,disrupted oral immunity in HIV-infected individuals leads to an imbalance between the oral microbiome and local immune responses,which may contribute to the development of HIV-related diseases and HIV-associated non-acquired immunodeficiency syndrome comorbidities.Although the introduction of antiretroviral therapy(ART)has led to a significant decrease in occurrence of the opportunistic oral infections in HIV-infected individuals,the dysbiosis in oral microbiome persists.Furthermore,several studies with the aim to investigate the ability of probiotics to regulate the dysbiosis of oral microbiota in HIV-infected individuals are ongoing.However,the effects of ART and probiotics on oral microbiome in HIV-infected individuals remain unclear.In this article,we review the composition of the oral microbiome in healthy and HIV-infected individuals and the possible effect of oral microbiome on HIV-associated oral diseases.We also discuss how ART and probiotics influence the oral microbiome in HIV infection.We believe that a deeper understanding of composition and function of the oral microbiome is critical for the development of effective preventive and therapeutic strategies for HIV infection展开更多
Oral diseases are associated with systemic diseases;such as type II diabetes, cardiovascular disease, rheumatoid arthritis (RA), and neurological diseases. Coincidentally, the oral microbiome (fluids or extracts) is r...Oral diseases are associated with systemic diseases;such as type II diabetes, cardiovascular disease, rheumatoid arthritis (RA), and neurological diseases. Coincidentally, the oral microbiome (fluids or extracts) is readily accessible and easily sampled;therefore, serving as a diagnostic or prognostic tool for health status. The oral microbiome is a useful research model for studying fundamental questions of the human microbiome. In this narrative literature review, we examine the characteristics of oral microorganisms, the relationship between oral microorganisms and human diseases, and the important role of oral microorganisms in disease prevention. Also, we illustrate the usefulness of sampling the oral microbiome in developing the diagnosis and prognostic treatment strategies for oral and systemic diseases to accelerate their clinical application. Selective saliva biomarkers and microbiome can serve for useful indices to oral diseases and systemic diseases, and as a model research tool, the oral cavity has many uses in the clinical and research environment. The relationships between oral health and systemic diseases are quite profound, and future research will illuminate opportunities for fruitful preventative measures and therapeutics.展开更多
Probiotics are living bacterial cells that have significant therapeutic potential for treating human infectious diseases.There is a huge market for probiotics in the pharmaceutical sector.They have been frequently use...Probiotics are living bacterial cells that have significant therapeutic potential for treating human infectious diseases.There is a huge market for probiotics in the pharmaceutical sector.They have been frequently used to treat the gastrointestinal diseases and improve gut immunity.In this review,the strains currently in use for manufacturing oral probiotic formulations are discussed.The review further recommends the use of probiotics for the control of various oral health disorders,like dental caries,periodontitis,gingivitis,halitosis,burning mouth syndrome,and oral cancer.Finally,this review also explores the use of various commercial probiotic products in maintaining oral health,their market values,and government acts and regulations that are relevant to the production and marketing of probiotics.Probiotics have tremendous therapeutic potential and more in-depth research must be done on these beneficial bacteria to make them one of the leading drugs in treating oral disorders.展开更多
文摘Bacterial endotoxins are a major concern in periodontal health and diseases owing to their structure and biological activity.With up-to-date knowledge of endotoxins and the recent findings about the influence of endotoxins in dental health,their probable mode of pathogenesis,and standard detection methods,this review analyzes the potential efficacy and benefits of probiotics in combination with conventional and contemporary treatment measures.In the oral cavity,Gram-negative bacteria are documented to predominate in the pulpal lesions with radiolucent areas and in the root canal with pulp necrosis,where they pose an absolute threat by promoting a series of inflammatory reactions.Endotoxin,a constituent of Gram-negative bacteria establishes a nexus between cytokine stimulation and proinflammatory reactions,therefore plays a critical role in decaying dental pulp and modulating periodontal diseases.Currently,the treatment regimen involves several biochemical preparations.In addition,probiotics have been reported to control endotoxin in gingivitis and contribute to the overall improvement of dental health.A potential benefit of a combination of probiotics as a complementary treatment along with the conventional treatment warrant more empirical evidence to elucidate its role and mechanism in resolving the clinical manifestations associated with endotoxins in the periodontal region.
基金supported by Chinese Academy of Medical Sciences(CAMS)Innovation Fund for Medical Sciences(2017-I2M1e011,China)the Drug Innovation Major Project(2018ZX09711001-002-005,China)+1 种基金National Natural Science Foundation of China(No.82073778,China)the Fundamental Research Funds for the Central Public Welfare Research Institutes(2018PT35002,China)
文摘Due to its safety,convenience,low cost and good compliance,oral administration attracts lots of attention.However,the efficacy of many oral drugs is limited to their unsatisfactory bioavailability in the gastrointestinal tract.One of the critical and most overlooked factors is the symbiotic gut microbiota that can modulate the bioavailability of oral drugs by participating in the biotransformation of oral drugs,influencing the drug transport process and altering some gastrointestinal properties.In this review,we summarized the existing research investigating the possible relationship between the gut microbiota and the bioavailability of oral drugs,which may provide great ideas and useful instructions for the design of novel drug delivery systems or the achievement of personalized medicine.
文摘The vaginal microbiota is less complex than the gut microbiota,and the colonization of Lactobacillus in the female vagina is considered to be critical for reproductive health.Oral probiotics have been suggested as promising means to modulate vaginal homeostasis in the general population.In this study,60 Chinese women were followed for over a year before,during,and after treatment with the probiotics Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14.Shotgun metagenomic data of 1334 samples from multiple body sites did not support a colonization route of the probiotics from the oral cavity to the intestinal tract and then to the vagina.Our analyses enable the classification of the cervicovaginal microbiome into a stable state and a state of dysbiosis.The microbiome in the stable group steadily maintained a relatively high abundance of Lactobacilli over one year,which was not affected by probiotic intake,whereas in the dysbiosis group,the microbiota was more diverse and changed markedly over time.Data from a subset of the dysbiosis group suggests this subgroup possibly benefited from supplementation with the probiotics,indicating that probiotics supplementation can be prescribed for women in a subclinical microbiome setting of dysbiosis,providing opportunities for targeted and personalized microbiome reconstitution.
文摘Background: Periodontal infections are the most common disease of cats and dogs. About 70% - 80% of them suffer from these infections during their lifetime. These infections occur in two forms, gingivitis and periodontitis. Gingivitis is a reversible inflammation of the gingiva which can be cured. As with humans, the harmful bacteria can cause infections also in organs such as the heart, liver and kidneys. Besides veterinary treatment, also the owner’s everyday care is essential. Objective: The aim of the study was to develop and clinically test easy home care probiotic product for cats’ and dogs’ oral health. Methods: Nine cats and thirteen dogs were given a combination of selected bacteria Streptococcus thermophilus SP4, Lactobacillus plantarum 14D and Lactobacillus rhamnosus SP1 (Petguide powder) in powder form daily for four weeks on top of the dry feed. Microbiological samples were taken before and after the intervention from each dog and cat. The samples were analyzed with flow cytometric analysis using Porphyromonas gingivalis and Fusobacterium sp. as indicators for oral health. Results: The home care treatment with these specific, live probiotic bacteria was shown to prevent the growth of infectious bacteria within four weeks. Petguide probiotic powder reduced the relative abundance of infectious microbes significantly (p = 0.005) in cats and dogs. Conclusion: The results suggest that the probiotic product with three live strains of probiotic bacteria prevents the growth of infectious bacterial species that are known to cause infections in pets’ mouths. Therefore, this probiotic combination could be used to improve the oral health of pets and increase their quality of life.
基金the National Natural Science Foundation of China(Nos.82072271,81772165,and 81974303)the National 13th Five-Year Grand Program on Key Infectious Disease Control(Nos.2017ZX10202101-004-001,2017ZX10202102-005-003)+1 种基金the NSFC-NIH Biomedical collaborative research program(No.81761128001)the Beijing Key Laboratory for HIV/AIDS Research(No.BZ0089)。
文摘A massive depletion of CD4+T lymphocytes has been described in early and acute human immunodeficiency virus(HIV)infection,leading to an imbalance between the human microbiome and immune responses.In recent years,a growing interest in the alterations in gut microbiota in HIV infection has led to many studies;however,only few studies have been conducted to explore the importance of oral microbiome in HIV-infected individuals.Evidence has indicated the dysbiosis of oral microbiota in people living with HIV(PLWH).Potential mechanisms might be related to the immunodeficiency in the oral cavity of HIV-infected individuals,including changes in secretory components such as reduced levels of enzymes and proteins in saliva and altered cellular components involved in the reduction and dysfunction of innate and adaptive immune cells.As a result,disrupted oral immunity in HIV-infected individuals leads to an imbalance between the oral microbiome and local immune responses,which may contribute to the development of HIV-related diseases and HIV-associated non-acquired immunodeficiency syndrome comorbidities.Although the introduction of antiretroviral therapy(ART)has led to a significant decrease in occurrence of the opportunistic oral infections in HIV-infected individuals,the dysbiosis in oral microbiome persists.Furthermore,several studies with the aim to investigate the ability of probiotics to regulate the dysbiosis of oral microbiota in HIV-infected individuals are ongoing.However,the effects of ART and probiotics on oral microbiome in HIV-infected individuals remain unclear.In this article,we review the composition of the oral microbiome in healthy and HIV-infected individuals and the possible effect of oral microbiome on HIV-associated oral diseases.We also discuss how ART and probiotics influence the oral microbiome in HIV infection.We believe that a deeper understanding of composition and function of the oral microbiome is critical for the development of effective preventive and therapeutic strategies for HIV infection
文摘Oral diseases are associated with systemic diseases;such as type II diabetes, cardiovascular disease, rheumatoid arthritis (RA), and neurological diseases. Coincidentally, the oral microbiome (fluids or extracts) is readily accessible and easily sampled;therefore, serving as a diagnostic or prognostic tool for health status. The oral microbiome is a useful research model for studying fundamental questions of the human microbiome. In this narrative literature review, we examine the characteristics of oral microorganisms, the relationship between oral microorganisms and human diseases, and the important role of oral microorganisms in disease prevention. Also, we illustrate the usefulness of sampling the oral microbiome in developing the diagnosis and prognostic treatment strategies for oral and systemic diseases to accelerate their clinical application. Selective saliva biomarkers and microbiome can serve for useful indices to oral diseases and systemic diseases, and as a model research tool, the oral cavity has many uses in the clinical and research environment. The relationships between oral health and systemic diseases are quite profound, and future research will illuminate opportunities for fruitful preventative measures and therapeutics.
文摘Probiotics are living bacterial cells that have significant therapeutic potential for treating human infectious diseases.There is a huge market for probiotics in the pharmaceutical sector.They have been frequently used to treat the gastrointestinal diseases and improve gut immunity.In this review,the strains currently in use for manufacturing oral probiotic formulations are discussed.The review further recommends the use of probiotics for the control of various oral health disorders,like dental caries,periodontitis,gingivitis,halitosis,burning mouth syndrome,and oral cancer.Finally,this review also explores the use of various commercial probiotic products in maintaining oral health,their market values,and government acts and regulations that are relevant to the production and marketing of probiotics.Probiotics have tremendous therapeutic potential and more in-depth research must be done on these beneficial bacteria to make them one of the leading drugs in treating oral disorders.
