To improve antagonistic metabolites production of Bacillus subtilis strain BS501a, physical parameters of fermentation and metal inorganic salts in medium, namely initial pH value, culture temperature, fermentation ti...To improve antagonistic metabolites production of Bacillus subtilis strain BS501a, physical parameters of fermentation and metal inorganic salts in medium, namely initial pH value, culture temperature, fermentation time, concentrations of CaC12, FeSO4, ZnSO4, MnSO4 and MgSO4, were optimized using one-factor-at-a-time and orthogonal tests. The results show that the optimal physical parameters of fermentation are an initial pH of 7.0, a culture temperature of 30 ~C, and a fermentation time of 48 h. The optimal concentrations of metal inorganic salts in basal medium are 10.2 mmol/L CaCl2, 0.4 mmol/L FeSO4, 3.5 mmol/L ZnSO4, 0.6 mmol/L MnSO4 and 2.0 mmol/L MgSO4. Among the metal inorganic salts, MgSO4 and MnSO4 play important roles in the improvement of the antagonistic metabolites production of B. subtilis strain BS501a; especially, MgSO4 contributes a highly significant effect. The average diameter of inhibition zone of the BS501a filtered fermentation supernatant （FFS） cultured in the optimal fermentation conditions against Magnaporthe grisea DWBJ329 reaches 71.4 mm, and there is 2.4-fold increase in antifungal activity as compared with 21.2 mm under the pre-optimized conditions.展开更多
30~300 MHz的甚高频(Very High Frequency,VHF)频段是重要的射电天文波段,该频段观测采用天线阵组阵方式。稀布阵列具有空间分辨率高、副瓣电平低以及造价低等优点,进一步的天线阵综合加权可以对天线阵主瓣波束进行有效赋形,对最大旁瓣...30~300 MHz的甚高频(Very High Frequency,VHF)频段是重要的射电天文波段,该频段观测采用天线阵组阵方式。稀布阵列具有空间分辨率高、副瓣电平低以及造价低等优点,进一步的天线阵综合加权可以对天线阵主瓣波束进行有效赋形,对最大旁瓣副瓣(Maximum Side Lobe)电平和远区栅瓣(Far Side Lobe)电平进行抑制。首先回顾了射电天文甚高频稀布阵列研究发展和现状,以及将会遇到的难点,提出了首先优化最优稀布天线阵元排布,进一步提出融合高性能计算平台+FPGA SOPC的稀布甚高频射电天文阵列信号处理结构体系,在图形处理器(Graphics Processing Unit,GPU)或者云计算平台上完成对天线阵各阵元频点加权参数的计算,然后通过高速总线将计算参数下发到前端的信号处理板中,通过FPGA SOPC完成对加权参数的配发。进一步分析计算了多波束情况下的数据率,可以实现实时的参数配置。本文成果为下一步大规模甚高频天线阵架设提供了技术依据。展开更多
An air parametric array can generate a highly directional beam of audible sound in air,which has a wide range of applications in targeted audio delivery.Capacitive micromachined ultrasonic transducer(CMUTs)have great ...An air parametric array can generate a highly directional beam of audible sound in air,which has a wide range of applications in targeted audio delivery.Capacitive micromachined ultrasonic transducer(CMUTs)have great potential for air-coupled applications,mainly because of their low acoustic impedance.In this study,an air-coupled CMUT array is designed as an air parametric array.A hexagonal array is proposed to improve the directivity of the sound generated.A finite element model of the CMUT is established in COMSOL software to facilitate the choice of appropriate structural parameters of the CMUT cell.The CMUT array is then fabricated by a wafer bonding process with high consistency.The performances of the CMUT are tested to verify the accuracy of the finite element analysis.By optimizing the component parameters of the bias-T circuit used for driving the CMUT,DC and AC voltages can be effectively applied to the top and bottom electrodes of the CMUT to provide efficient ultrasound transmission.Finally,the prepared hexagonal array is successfully used to conduct preliminary experiments on its application as an air parametric array.展开更多
An acoustic vector sensor can measure the components of particle velocity and the acoustic pressure at the same point simultaneously, which provides a larger array gain against the ambient noise and a higher angular r...An acoustic vector sensor can measure the components of particle velocity and the acoustic pressure at the same point simultaneously, which provides a larger array gain against the ambient noise and a higher angular resolution than the omnidirectional pressure sensor. This paper presents an experimental study of array gain for a conformal acoustic vector sensor array in a practical environment. First, the manifold vector is calculated using the real measured data so that the effects of array mismatches can be minimized. Second, an optimal beamformer with a specific spatial response on the basis of the stable directivity of the ambient noise is designed, which can effectively suppress the ambient noise. Experimental results show that this beamformer for the conformal acoustic vector sensor array provides good signal-to- noise ratio enhancement and is more advantageous than the delay-and-sum and minimum variance distortionless response beamformers.展开更多
基金Project(2010A210003) supported by Henan Province Natural Sciences Research PlanProject(0910SGYS34370-2) supported by Zhengzhou City Science and Technology Research PlanProject supported by the Youth Backbone Teacher of Universities in Henan Province Grants Plan
文摘To improve antagonistic metabolites production of Bacillus subtilis strain BS501a, physical parameters of fermentation and metal inorganic salts in medium, namely initial pH value, culture temperature, fermentation time, concentrations of CaC12, FeSO4, ZnSO4, MnSO4 and MgSO4, were optimized using one-factor-at-a-time and orthogonal tests. The results show that the optimal physical parameters of fermentation are an initial pH of 7.0, a culture temperature of 30 ~C, and a fermentation time of 48 h. The optimal concentrations of metal inorganic salts in basal medium are 10.2 mmol/L CaCl2, 0.4 mmol/L FeSO4, 3.5 mmol/L ZnSO4, 0.6 mmol/L MnSO4 and 2.0 mmol/L MgSO4. Among the metal inorganic salts, MgSO4 and MnSO4 play important roles in the improvement of the antagonistic metabolites production of B. subtilis strain BS501a; especially, MgSO4 contributes a highly significant effect. The average diameter of inhibition zone of the BS501a filtered fermentation supernatant （FFS） cultured in the optimal fermentation conditions against Magnaporthe grisea DWBJ329 reaches 71.4 mm, and there is 2.4-fold increase in antifungal activity as compared with 21.2 mm under the pre-optimized conditions.
文摘30~300 MHz的甚高频(Very High Frequency,VHF)频段是重要的射电天文波段,该频段观测采用天线阵组阵方式。稀布阵列具有空间分辨率高、副瓣电平低以及造价低等优点,进一步的天线阵综合加权可以对天线阵主瓣波束进行有效赋形,对最大旁瓣副瓣(Maximum Side Lobe)电平和远区栅瓣(Far Side Lobe)电平进行抑制。首先回顾了射电天文甚高频稀布阵列研究发展和现状,以及将会遇到的难点,提出了首先优化最优稀布天线阵元排布,进一步提出融合高性能计算平台+FPGA SOPC的稀布甚高频射电天文阵列信号处理结构体系,在图形处理器(Graphics Processing Unit,GPU)或者云计算平台上完成对天线阵各阵元频点加权参数的计算,然后通过高速总线将计算参数下发到前端的信号处理板中,通过FPGA SOPC完成对加权参数的配发。进一步分析计算了多波束情况下的数据率,可以实现实时的参数配置。本文成果为下一步大规模甚高频天线阵架设提供了技术依据。
基金the National Key R&D Program of China(Nos.2017YFA0205103 and 2018YFE020505)the National Natural Science Foundation of China(Nos.81571766 and 61771337)+1 种基金the Natural Science Foundation of Tianjin,China(No.17JCYBJC24400)the“111”Project of China(No.B07014).
文摘An air parametric array can generate a highly directional beam of audible sound in air,which has a wide range of applications in targeted audio delivery.Capacitive micromachined ultrasonic transducer(CMUTs)have great potential for air-coupled applications,mainly because of their low acoustic impedance.In this study,an air-coupled CMUT array is designed as an air parametric array.A hexagonal array is proposed to improve the directivity of the sound generated.A finite element model of the CMUT is established in COMSOL software to facilitate the choice of appropriate structural parameters of the CMUT cell.The CMUT array is then fabricated by a wafer bonding process with high consistency.The performances of the CMUT are tested to verify the accuracy of the finite element analysis.By optimizing the component parameters of the bias-T circuit used for driving the CMUT,DC and AC voltages can be effectively applied to the top and bottom electrodes of the CMUT to provide efficient ultrasound transmission.Finally,the prepared hexagonal array is successfully used to conduct preliminary experiments on its application as an air parametric array.
基金Project supported by the China Postdoctoral Science Foundation(Grant No.2016M592782)the National Natural Science Foundation of China(Grant Nos.11274253 and 11604259)
文摘An acoustic vector sensor can measure the components of particle velocity and the acoustic pressure at the same point simultaneously, which provides a larger array gain against the ambient noise and a higher angular resolution than the omnidirectional pressure sensor. This paper presents an experimental study of array gain for a conformal acoustic vector sensor array in a practical environment. First, the manifold vector is calculated using the real measured data so that the effects of array mismatches can be minimized. Second, an optimal beamformer with a specific spatial response on the basis of the stable directivity of the ambient noise is designed, which can effectively suppress the ambient noise. Experimental results show that this beamformer for the conformal acoustic vector sensor array provides good signal-to- noise ratio enhancement and is more advantageous than the delay-and-sum and minimum variance distortionless response beamformers.
