There are increasing concerns on the environmental impacts of intensive chemical agriculture. The effect of high agrochemical inputs used in intensive chemical farming was assessed on soil microbiological, molecular a...There are increasing concerns on the environmental impacts of intensive chemical agriculture. The effect of high agrochemical inputs used in intensive chemical farming was assessed on soil microbiological, molecular and biochemical properties in tropical Vertisols in India. Farm field sites under normal cultivation of arable crops using high inputs of fertilizers and pesticides in chili （Capsicum annum L., 5.0× dose for fertilizers and 1.5× dose for pesticides over normal inputs） and black gram （Vigna mungo L. Hepper, 2.2× dose for fertilizers and 2.3× dose for pesticides over normal inputs） were compared with adjacent sites using normal recommended doses. Organic carbon and basal respiration showed no response to high inputs of fertilizers and pesticides in soils of both crops. Labile carbon decreased by 10% in chili soils and increased by 24% in black gram soils under high input farming system. The proportion of soil labile carbon as a fraction of soil organic carbon was unaffected by high inputs. The labile carbon mineralization coefficient （qMLc） increased by 50.0% in chili soils, indicating that the soil microorganisms were under stress due to high agochemical inputs, whereas qMLc decreased by 36.4% in black gram soils. Copiotrophs increased due to high inputs in soils of both chili （63.1%） and black gram （47.1%）. Oligotrophs increased by 10.8% in black gram soils but not in chili soils. The abundance of amoA gene reduced by 39.3% in chili soils due to high inputs and increased significantly by 110.8% in black gram soils. β-Glucosidase also increased by 27.2% and 325.0%, respectively. Acid phosphatase activity reduced by 29.2% due to high inputs in chili soils and increased by 105.0% in black gram soils. The use of high agrochemical inputs thus had adverse consequences on biological health in chili but not in black gram soils. In soils cultivated with black gram, the moderating effect of cultivating legumes and their beneficial effect on soil health were evident from the increase in 展开更多
Phytoplankton assemblages in the subtrophical oligotrophic Lake Fuxian, the second deepest lake in China, were investigated monthly from September 2002 to August 2003. A total of 113 species belonging to seven phyla w...Phytoplankton assemblages in the subtrophical oligotrophic Lake Fuxian, the second deepest lake in China, were investigated monthly from September 2002 to August 2003. A total of 113 species belonging to seven phyla were identified, among them, a filamentous green alga, Mougeotia sp., dominated almost throughout the study period and comprised most of the total phytoplankton biomass. Mougeotia sp. has made a substantial development during the past decades: it was absent in 1957, only occasionally present in 1983, increased substantially in 1993, and became predominant in 2002—2003. It is likely that natural invasion of the Taihu Lake noodlefish(Neosalanx taihuensis) has led to a change of dominant herbivorous zooplankton from small to large calanoid, which has increased grazing pressure on small edible algae, and thus has indirectly favored the development of the inedible filamentous Mougeotia sp.展开更多
The effects of precipitation reduction and nitrogen deposition increase on soil bacterial communities and functions impact soil nitrogen cycling. Seasonal changes could modify the effects of precipitation reduction an...The effects of precipitation reduction and nitrogen deposition increase on soil bacterial communities and functions impact soil nitrogen cycling. Seasonal changes could modify the effects of precipitation reduction and nitrogen deposition increase on bacterial communities and functions by changing soil environments and properties. Understanding soil microbial communities and the seasonal response of functions to precipitation reduction and nitrogen deposition increase may be important for the accurate prediction of changes in the soil nitrogen dynamics. Thus, a long-term field simulation experiment of nitrogen deposition increase and throughfall exclusion was established to investigate soil bacterial communities’ response to nitrogen deposition increase and/or precipitation reduction, with no nitrogen deposition increase and no precipation reduction as a control, in a temperate forest. We examined soil bacterial communities(Illumina sequencing) under different treatments during the winter, freezing-thawing cycle periods(FTCs), and growing season. The bacterial functional groups were predicted by the FAPROTAX database. The results showed that nitrogen deposition increase, precipitation reduction, the combined effect of nitrogen deposition increase and precipitation reduction, and seasonal changes significantly altered the soil bacterial community composition.Interestingly, by combining the result of a previous study in which nitrogen deposition increase increased the nitrous oxide flux in the same experimental system, the loss of soil nitrogen was increased by the decrease in denitrification and increase of nitrification bacteria under nitrogen deposition increase,while ammonification bacteria significantly increased and N-fixing bacteria significantly decreased with precipitation reduction compared to the control. In relation to seasonal changes, the aromatic-degrading, cellulolytic, and ureolytic bacteria were lowest during FTCs, which indicated that FTCs might inhibit biodegradation. Nitrification and nitrite展开更多
The physico-chemical water quality of the Obafemi Awolowo University Teaching and Research Farm Lake, Ile-Ife, Nigeria, was studied over an annual cycle (September 2006 to August 2007) almost 40 years after its impoun...The physico-chemical water quality of the Obafemi Awolowo University Teaching and Research Farm Lake, Ile-Ife, Nigeria, was studied over an annual cycle (September 2006 to August 2007) almost 40 years after its impoundment in 1967. This was with a view to obtaining the then current state of physico-chemical water quality parameters with their variations in time and space as well as using some of the data obtained to evaluate the aging process of the lake for the 40 years of its existence. There was no significant (p > 0.05) horizontal variation (i.e. upstream-dowstream) in the physico-chemical characteristics of the lake whereas, Apparent-color, True color, Turbidity, Total Suspended Solids (TSS), Total Solids (TS) and Total Acidity increased significantly (p < 0.05) from the lake surface towards the bottom of the lake. On the other hand, water pH, Dissolved Oxygen (DO);Dissolved Oxygen Saturation (DO% Sat.) and Biochemical Oxygen Demand (BOD5) decreased significantly (p < 0.05) from the lake surface towards the bottom of the lake. Dissolved Oxygen (DO), Dissolved Oxygen Saturation (DO% Sat.) and Nitrate ion (NO-3) were significantly higher (p < 0.05) in the dry season than in the rainy season while, True colour, pH, Conductivity, Total Hardness, Calcium ion (Ca2+), Magnesium ion (Mg2+), Potassium ion (K+) and Sulphate ion (SO2-4) were significantly higher (p < 0.05) in the rainy season than in the dry season. The lake water could be regarded as shallow, coloured, turbid, less transparent, slightly acidic, soft and well-buffered fresh-waterbodies with evidence of aging (i.e. transforming the lake from an oligotrophic status to a eutrophic waterbody). The results indicated that the lake is suitable for most of its applications with little or no adjustments as the case may be;except probably for drinking purposes where some microbial analysis may be necessary.展开更多
An ancient irrigated paddy soil from the Neolithic age was excavated at Chuodunshan Site in the Yangtze River Delta, close to Suzhou, China. The soil organic matter (SOM) content in the prehistoric rice soil is compar...An ancient irrigated paddy soil from the Neolithic age was excavated at Chuodunshan Site in the Yangtze River Delta, close to Suzhou, China. The soil organic matter (SOM) content in the prehistoric rice soil is comparable to the average SOM content of present rice soils in this region, but it is about 5 times higher than that in the parent materials. As possible biomarkers to indicate the presence of the prehistoric paddy soil, the bacterial communities were investigated using the techniques of aerobic and anaerobic oligotrophic bacteria enumeration, Biolog analysis, and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that in the buried soil layers, the prehistoric paddy soil had the largest number of aerobic and anaerobic oligotrophic bacteria, up to 6.12 and 5.86 log cfu g-1 dry soil, respectively. The prehistoric paddy soil displayed better carbon utilization potential and higher functional diversity compared to the parent materials and a prehistoric loess layer. The Shannon index and richness based on DGGE profiles of bacterial 16S rRNA genes were higher in prehistoric paddy soil than those in the prehistoric loess soil. It might be concluded that the prehistoric irrigated rice cultivation accumulated the SOM in plowed soil layer, and thus increased soil bacterial populations, metabolic activity, functional diversity and genetic diversity. Bacterial communities might be considered as the sensitive indicators of the presence of the prehistoric paddy soil in China's Yangtze River Delta.展开更多
基金supported by the Indian Council of Agricultural Research,New Delhi,India
文摘There are increasing concerns on the environmental impacts of intensive chemical agriculture. The effect of high agrochemical inputs used in intensive chemical farming was assessed on soil microbiological, molecular and biochemical properties in tropical Vertisols in India. Farm field sites under normal cultivation of arable crops using high inputs of fertilizers and pesticides in chili （Capsicum annum L., 5.0× dose for fertilizers and 1.5× dose for pesticides over normal inputs） and black gram （Vigna mungo L. Hepper, 2.2× dose for fertilizers and 2.3× dose for pesticides over normal inputs） were compared with adjacent sites using normal recommended doses. Organic carbon and basal respiration showed no response to high inputs of fertilizers and pesticides in soils of both crops. Labile carbon decreased by 10% in chili soils and increased by 24% in black gram soils under high input farming system. The proportion of soil labile carbon as a fraction of soil organic carbon was unaffected by high inputs. The labile carbon mineralization coefficient （qMLc） increased by 50.0% in chili soils, indicating that the soil microorganisms were under stress due to high agochemical inputs, whereas qMLc decreased by 36.4% in black gram soils. Copiotrophs increased due to high inputs in soils of both chili （63.1%） and black gram （47.1%）. Oligotrophs increased by 10.8% in black gram soils but not in chili soils. The abundance of amoA gene reduced by 39.3% in chili soils due to high inputs and increased significantly by 110.8% in black gram soils. β-Glucosidase also increased by 27.2% and 325.0%, respectively. Acid phosphatase activity reduced by 29.2% due to high inputs in chili soils and increased by 105.0% in black gram soils. The use of high agrochemical inputs thus had adverse consequences on biological health in chili but not in black gram soils. In soils cultivated with black gram, the moderating effect of cultivating legumes and their beneficial effect on soil health were evident from the increase in
基金The Key Project of Chinese Academy of Sciences(No. KSCX1 SW 13).
文摘Phytoplankton assemblages in the subtrophical oligotrophic Lake Fuxian, the second deepest lake in China, were investigated monthly from September 2002 to August 2003. A total of 113 species belonging to seven phyla were identified, among them, a filamentous green alga, Mougeotia sp., dominated almost throughout the study period and comprised most of the total phytoplankton biomass. Mougeotia sp. has made a substantial development during the past decades: it was absent in 1957, only occasionally present in 1983, increased substantially in 1993, and became predominant in 2002—2003. It is likely that natural invasion of the Taihu Lake noodlefish(Neosalanx taihuensis) has led to a change of dominant herbivorous zooplankton from small to large calanoid, which has increased grazing pressure on small edible algae, and thus has indirectly favored the development of the inedible filamentous Mougeotia sp.
基金This research was part of the project Global Change and Response which is supported by the National Key Research and Development Program of China(No.2016YFA0600800)and the National Natural Science Foundation of China(Nos.41773075,41575137,31370494,and 31170421).
文摘The effects of precipitation reduction and nitrogen deposition increase on soil bacterial communities and functions impact soil nitrogen cycling. Seasonal changes could modify the effects of precipitation reduction and nitrogen deposition increase on bacterial communities and functions by changing soil environments and properties. Understanding soil microbial communities and the seasonal response of functions to precipitation reduction and nitrogen deposition increase may be important for the accurate prediction of changes in the soil nitrogen dynamics. Thus, a long-term field simulation experiment of nitrogen deposition increase and throughfall exclusion was established to investigate soil bacterial communities’ response to nitrogen deposition increase and/or precipitation reduction, with no nitrogen deposition increase and no precipation reduction as a control, in a temperate forest. We examined soil bacterial communities(Illumina sequencing) under different treatments during the winter, freezing-thawing cycle periods(FTCs), and growing season. The bacterial functional groups were predicted by the FAPROTAX database. The results showed that nitrogen deposition increase, precipitation reduction, the combined effect of nitrogen deposition increase and precipitation reduction, and seasonal changes significantly altered the soil bacterial community composition.Interestingly, by combining the result of a previous study in which nitrogen deposition increase increased the nitrous oxide flux in the same experimental system, the loss of soil nitrogen was increased by the decrease in denitrification and increase of nitrification bacteria under nitrogen deposition increase,while ammonification bacteria significantly increased and N-fixing bacteria significantly decreased with precipitation reduction compared to the control. In relation to seasonal changes, the aromatic-degrading, cellulolytic, and ureolytic bacteria were lowest during FTCs, which indicated that FTCs might inhibit biodegradation. Nitrification and nitrite
文摘The physico-chemical water quality of the Obafemi Awolowo University Teaching and Research Farm Lake, Ile-Ife, Nigeria, was studied over an annual cycle (September 2006 to August 2007) almost 40 years after its impoundment in 1967. This was with a view to obtaining the then current state of physico-chemical water quality parameters with their variations in time and space as well as using some of the data obtained to evaluate the aging process of the lake for the 40 years of its existence. There was no significant (p > 0.05) horizontal variation (i.e. upstream-dowstream) in the physico-chemical characteristics of the lake whereas, Apparent-color, True color, Turbidity, Total Suspended Solids (TSS), Total Solids (TS) and Total Acidity increased significantly (p < 0.05) from the lake surface towards the bottom of the lake. On the other hand, water pH, Dissolved Oxygen (DO);Dissolved Oxygen Saturation (DO% Sat.) and Biochemical Oxygen Demand (BOD5) decreased significantly (p < 0.05) from the lake surface towards the bottom of the lake. Dissolved Oxygen (DO), Dissolved Oxygen Saturation (DO% Sat.) and Nitrate ion (NO-3) were significantly higher (p < 0.05) in the dry season than in the rainy season while, True colour, pH, Conductivity, Total Hardness, Calcium ion (Ca2+), Magnesium ion (Mg2+), Potassium ion (K+) and Sulphate ion (SO2-4) were significantly higher (p < 0.05) in the rainy season than in the dry season. The lake water could be regarded as shallow, coloured, turbid, less transparent, slightly acidic, soft and well-buffered fresh-waterbodies with evidence of aging (i.e. transforming the lake from an oligotrophic status to a eutrophic waterbody). The results indicated that the lake is suitable for most of its applications with little or no adjustments as the case may be;except probably for drinking purposes where some microbial analysis may be necessary.
基金Supported by the National Natural Science Foundation of China (No.40335047)the Sino-German Center for Research Promotion in Beijing (No.GZ 518)
文摘An ancient irrigated paddy soil from the Neolithic age was excavated at Chuodunshan Site in the Yangtze River Delta, close to Suzhou, China. The soil organic matter (SOM) content in the prehistoric rice soil is comparable to the average SOM content of present rice soils in this region, but it is about 5 times higher than that in the parent materials. As possible biomarkers to indicate the presence of the prehistoric paddy soil, the bacterial communities were investigated using the techniques of aerobic and anaerobic oligotrophic bacteria enumeration, Biolog analysis, and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that in the buried soil layers, the prehistoric paddy soil had the largest number of aerobic and anaerobic oligotrophic bacteria, up to 6.12 and 5.86 log cfu g-1 dry soil, respectively. The prehistoric paddy soil displayed better carbon utilization potential and higher functional diversity compared to the parent materials and a prehistoric loess layer. The Shannon index and richness based on DGGE profiles of bacterial 16S rRNA genes were higher in prehistoric paddy soil than those in the prehistoric loess soil. It might be concluded that the prehistoric irrigated rice cultivation accumulated the SOM in plowed soil layer, and thus increased soil bacterial populations, metabolic activity, functional diversity and genetic diversity. Bacterial communities might be considered as the sensitive indicators of the presence of the prehistoric paddy soil in China's Yangtze River Delta.
