A new rearranged charnigrane sesquiterpene, named laurenokamurin, was isolated from the marine red alga Laurencia okamurai. Its structure was determined on the basis of spectroscopic methods.
To establish a micropropagation system of three Laurencia complex species （Laurencia okamurai, Laurencia tristicha, and Chondrophvcus undulatus） by tissue culture techniques, we studied the regeneration characterist...To establish a micropropagation system of three Laurencia complex species （Laurencia okamurai, Laurencia tristicha, and Chondrophvcus undulatus） by tissue culture techniques, we studied the regeneration characteristics and optimal culture conditions of axenic algal fragments cultured on solid medium and in liquid medium. Regeneration structures were observed and counted regularly under a reverse microscope to investigate the regeneration process, polarity and optimal illumination, and temperature and salinity levels. The results show that in most cultures of the three species, we obtained bud regeneration on solidified medium with 0.5% agar and in liquid medium. Rhizoid-like regeneration was filamentous and developed from the lower cut surface of fragments in L. okamurai, but was discoid and developed from the apical back side of bud regeneration in L. tristicha and C. undulatus. Regeneration polarity was localized to the apical part of algal fronds in all three species, and on fragments cut from the basal part of algae buds could develop from both the upper and the lower cut surfaces. Buds could develop from both the medullary and the cortical portions in L. okamurai and C. undulatus, while in L. tristicha, buds only emerged from the cortex. The optimal culture conditions for L. okamurai were 4 500 lx, 20℃ and 35 （salinity）; for C. undulatus, 4 500 lx, 20℃ and 30; and for L. tristicha, 4 500 lx, 25℃ and 30.展开更多
基金supported by the National High-Tech R & D Project(No.2007AA09Z403)the National Natural Science Foundation of China(No.30530080)the Department of Science and Technology of Shandong Province(No.2006GG2205023).
文摘A new rearranged charnigrane sesquiterpene, named laurenokamurin, was isolated from the marine red alga Laurencia okamurai. Its structure was determined on the basis of spectroscopic methods.
基金Supported by the fund from Jiangsu Key Laboratory of Marine Biotechnology (No. 2006HS006)Huaihai Institute of Technology, Lianyungang, China211-Project Funding of Soochow University (No. 14134908)
文摘To establish a micropropagation system of three Laurencia complex species （Laurencia okamurai, Laurencia tristicha, and Chondrophvcus undulatus） by tissue culture techniques, we studied the regeneration characteristics and optimal culture conditions of axenic algal fragments cultured on solid medium and in liquid medium. Regeneration structures were observed and counted regularly under a reverse microscope to investigate the regeneration process, polarity and optimal illumination, and temperature and salinity levels. The results show that in most cultures of the three species, we obtained bud regeneration on solidified medium with 0.5% agar and in liquid medium. Rhizoid-like regeneration was filamentous and developed from the lower cut surface of fragments in L. okamurai, but was discoid and developed from the apical back side of bud regeneration in L. tristicha and C. undulatus. Regeneration polarity was localized to the apical part of algal fronds in all three species, and on fragments cut from the basal part of algae buds could develop from both the upper and the lower cut surfaces. Buds could develop from both the medullary and the cortical portions in L. okamurai and C. undulatus, while in L. tristicha, buds only emerged from the cortex. The optimal culture conditions for L. okamurai were 4 500 lx, 20℃ and 35 （salinity）; for C. undulatus, 4 500 lx, 20℃ and 30; and for L. tristicha, 4 500 lx, 25℃ and 30.
文摘目的对采自中国东海南麂岛海域的海洋红藻冈村凹顶藻(Laurencia okamurai)进行系统的化学成分研究,并对得到的单体化合物进行抗菌活性筛选。方法采用正相硅胶柱色谱、凝胶Sephadex LH-20以及半制备高效液相色谱HPLC等层析手段对冈村凹顶藻的甲醇提取物进行了系统的分离纯化,同时运用1 H NMR、13 C NMR、EIMS等光谱技术手段对得到的化合物进行结构鉴定,并利用无乳链球菌、鳗弧菌、嗜水气单胞菌、塔氏弧菌、海豚链球菌、鮰爱德华和HN016对所得单体化合物进行抗菌活性测试。结果从冈村凹顶藻中分离得到了11个化合物,包括7个倍半萜化合物、1个甾体化合物、1个不饱和脂肪酸、1个脂类化合物和1个醛类化合物,分别是aplysin(1),debromolaurinterol(2),aplysinol(3),debromoisolaurinterol(4),3β-hydroperoxyaplysin(5),laurinterol acetate(6),Laurokomurenene A(7),cholesterol(8),棕榈油酸(9),octadec-3(E)-en-2-one(10)和(2E)-2-tridecyl-2-heptadecenal(11)。其中化合物2对无乳链球菌和鳗弧菌HLPF表现出强的特异性抑制活性,其余化合物均无明显的抗菌活性。结论本研究首次发现化合物2对无乳链球菌和鳗弧菌显示出很好的特异性抑制活性。
基金This project was supported by the National Science & Technology Major Project (No. 2009ZX09301-001)Natural Science Foundation of China (Nos. 40976048,30730108 and 20721003)+1 种基金STCSM International Cooperation Project between SIMM/China and ICB/Italy (No. 10540702900)partly founded by the NSFC-TRF International Cooperation Project (No. 20911140471)~~
