As a rare and valuable wood and herbal material, Dalbergia odorifera is often counterfeited by Dalbergia stevensonii in the market. For the confident chemical identification of D. odorifera and D. stevensonii, the eth...As a rare and valuable wood and herbal material, Dalbergia odorifera is often counterfeited by Dalbergia stevensonii in the market. For the confident chemical identification of D. odorifera and D. stevensonii, the ethanol-benzene extractives are characterized by multiple metabolomics tools, including Fourier transform infrared （FTIR） spectroscopy, nuclear magnetic resonance （NMR）, and gas chromatography- mass spectrometry （GC/MS）. Conventional FTIR spectroscopy, second derivative infrared （SD-IR） spectroscopy and two-dimensional correlation infrared （2D-IR） spectroscopy are combined to interpret the functional groups of the ethanol-benzene extractives. Fingerprint-like characteristics make FTIR a rapid and accurate method to distinguish D. odor/fera from D. stevensonii. Chemical differences of the extractives revealed by FrIR methods can be further confirmed by ^1H NMR and ^13C NMR. Meanwhile, the volatile compounds in the extractives can be identified by GC/MS. The combination of FTIR, NMR and GC/ MS makes it possible to obtain the multiple profiles of the ethanol-benzene extractives, which is essential for the confident chemical identification of D. odorifera and D. stevensonii.展开更多
Objective:Dalbergia odorifera has long been used as a Chinese herbal medicine for the treatment of cardiovascular and cerebrovascular diseases.This study aimed to determine the potential myocardial protective effect a...Objective:Dalbergia odorifera has long been used as a Chinese herbal medicine for the treatment of cardiovascular and cerebrovascular diseases.This study aimed to determine the potential myocardial protective effect and possible mechanism of action of D.odor ife ra essential oil(DOEO).Materials and Methods:The essential oil of D.odorifera was extracted by hydrodistillation.The cardioprotective effects of DOEO were examined by histopathological observation,myocardial enzyme detection,peroxidation,anti-oxidant level detection,and related protein expression.The compounds in the blood were identified by gas chromatography-mass spectrometry.Results:These results showed that DOEO had significant myocardial cell protection,with IC50 values ranging from 17.64 to 24.78μg/mL in vitro.Compared to the myocardial ischemia group,the DOEO pretreatment groups had lower levels of myocardial injury,creatinine kinase,lactate dehydrogenase,alanine transaminase,aspartate transaminase,hydrogen peroxide,and nitric oxide,and higher levels of glutathione and superoxide dismutase.In addition,DOEO pretreatment significantly increased Na+-K+-ATPase and Ca2+-ATPase levels.Moreove r,immunohistochemical e xperiments showed that DOEO remarkably increased the protein levels of NF-E2-related nuclear factor 2(Nrf2)and heme oxygenase-1(HO-1)and reduced the expression of apoptotic caspases,including caspase 3 and caspase 9.The main components of the blood were transnerolidol and nerolidol oxide.Overall,the study showed that DOEO displayed myocardial protection by upregulating the NF-E2-related nuclear factor-antioxidant response element(Nrf2-ARE)and caspase pathways.DOEO has a therapeutic effect on MI by inhibiting the oxidant and apoptotic effects.Conclusions:D.odorifera may be a potential candidate drug for treating myocardial ischemic injury.展开更多
The attenuation function of Dalbergia odorifera leaves on cerebral ischemia-reperfusion(I/R)is little known.The candidate targets for the Chinese herb were extracted from brain tissues through the high-affinity chroma...The attenuation function of Dalbergia odorifera leaves on cerebral ischemia-reperfusion(I/R)is little known.The candidate targets for the Chinese herb were extracted from brain tissues through the high-affinity chromatography.The molecular mechanism of D.odorifera leaves on cerebral I/R was investigated.Methods:Serial affinity chromatography based on D.odorifera leaves extract(DLE)affinity matrices were applied to find specific binding proteins in the brain tissues implemented on C57BL/6 mice by intraluminal middle cerebral artery occlusion for 1 h and reperfusion for 24 h.Specific binding proteins were subjected to mass-spectrometry to search for the differentially expressed proteins between control and DLE-affinity matrices.The hub genes were screened based on weighted gene co-expression network analysis(WGCNA).Then,predictive biology and potential experimental verification were performed for the candidate genes.The protective role of DLE in blood-brain barrier damage in cerebral I/R mice was evaluated by the leakage of Evans blue,western blotting,immunohistochemistry,and immunofluorescent staining.Results:952 differentially expressed proteins were classified into seven modules based on WGCNA under soft threshold 6.Based on WGCNA,AKT1,PIK3CA,NOS3,SMAD3,SMAD1,IL6,MAPK1,TGFBR2,TGFBR1,MAPK3,IGF1R,LRG1,mTOR,ROCK1,TGFB1,IL1B,SMAD2,and SMAD518 candidate hub proteins were involved in turquoise module.TGF-β,MAPK,focal adhesion,and adherens junction signaling pathway were associated with candidate hub proteins.Gene ontology analysis demonstrated that candidate hub proteins were related to the TGF-βreceptor signaling pathway,common-partner SMAD protein phosphorylation,etc.DLE could significantly reduce the leakage of Evans blue in mice with cerebral I/R,while attenuating the expression of occludin,claudin-5,and zonula occludens-1.Western blotting demonstrated that regulation of TGF-β/SMAD signaling pathway played an essential role in the protective effect of DLE.Conclusion:Thus,a number of candidate hub proteins were 展开更多
Dalbergia odorifera is particularly scarce,and the D. odorifera furniture and commercial raw materials on the market are mainly old furniture and agricultural tools,as well as commercially collected D. odorifera logs....Dalbergia odorifera is particularly scarce,and the D. odorifera furniture and commercial raw materials on the market are mainly old furniture and agricultural tools,as well as commercially collected D. odorifera logs. The logs and high-grade furniture made from D. odorifera are extremely expensive. It takes several decades for D. odorifera to grow into wood. At present,there is only a small area of newly planted and regenerated forest,and the planting area of D. odorifera is about 15 000 ha in Hainan Island,distributed in the low altitude area of Hainan Island. It is suggested that Hainan D. odorifera island should be built through the transformation of low-yielding forest land,the use of wasteland and marginal cultivated land,land around houses,green belt and roadside land for the cultivation of D. odorifera,and the development area can be up to 100 000-150 000 ha. It is necessary to mobilize farmers to revitalize the rural green economy,plant D. odorifera,beautify the environment,and increase the economic resources of rural trees; actively publicize the legal nature and obligation of D. odorifera protection,and create a strong atmosphere for each family to plant and protect D. odorifera.展开更多
Tar spot of Dalbergia odorifera, caused by Phyllachora dalbergiicola, is a common disease, and se-riously affected the rate of photosynthesis. Here we developed a species-specific Nested-PCR approach for rapidand accu...Tar spot of Dalbergia odorifera, caused by Phyllachora dalbergiicola, is a common disease, and se-riously affected the rate of photosynthesis. Here we developed a species-specific Nested-PCR approach for rapidand accurate detection of P. dalbergiicola, based on the differences in internal transcribed spacer （ITS） se-quences of P. dalbergiicola and another P. spp., an endophytic fungus, from which a pair of species-specificprimers P1/P2 （P1 ： 5＂-CGAGGTCAGAATCAAACG-3＂, P2： 5＂-TGAAGAACGCAGCGAAAT-3＂）, was designed.P1/P2 amplified only a unique 273 bp band from the genomic DNA of P. dalbergiicola. A Nested-PCR proce-dure using ITS4/ITS5 as the first-round primers, followed by P1/P2 primers, increased detection sensitivity10 000 fold to 100 ag. Using the Fast DNA-kit to extract DNA from the diseased plant tissues, the detection ofthe pathogen by Nested-PCR assay could be completed within I day. The results suggested that the PCR-basedmethods here could simplify both plant disease diagnosis and pathogen detection.展开更多
基金supported by the National Natural Science Foundation of China(No.31670564)
文摘As a rare and valuable wood and herbal material, Dalbergia odorifera is often counterfeited by Dalbergia stevensonii in the market. For the confident chemical identification of D. odorifera and D. stevensonii, the ethanol-benzene extractives are characterized by multiple metabolomics tools, including Fourier transform infrared （FTIR） spectroscopy, nuclear magnetic resonance （NMR）, and gas chromatography- mass spectrometry （GC/MS）. Conventional FTIR spectroscopy, second derivative infrared （SD-IR） spectroscopy and two-dimensional correlation infrared （2D-IR） spectroscopy are combined to interpret the functional groups of the ethanol-benzene extractives. Fingerprint-like characteristics make FTIR a rapid and accurate method to distinguish D. odor/fera from D. stevensonii. Chemical differences of the extractives revealed by FrIR methods can be further confirmed by ^1H NMR and ^13C NMR. Meanwhile, the volatile compounds in the extractives can be identified by GC/MS. The combination of FTIR, NMR and GC/ MS makes it possible to obtain the multiple profiles of the ethanol-benzene extractives, which is essential for the confident chemical identification of D. odorifera and D. stevensonii.
基金supported by the Hainan Province Science and Technology Special Fund(ZDYF2023SHFZ141,ZDYF2018123,ZDYF2021SHFZ077)the CAMS Innovation Fund for Medical Sciences(2021-I2M-1-032)Natural Science Foundation of Hainan Province,China(2019RC344)。
文摘Objective:Dalbergia odorifera has long been used as a Chinese herbal medicine for the treatment of cardiovascular and cerebrovascular diseases.This study aimed to determine the potential myocardial protective effect and possible mechanism of action of D.odor ife ra essential oil(DOEO).Materials and Methods:The essential oil of D.odorifera was extracted by hydrodistillation.The cardioprotective effects of DOEO were examined by histopathological observation,myocardial enzyme detection,peroxidation,anti-oxidant level detection,and related protein expression.The compounds in the blood were identified by gas chromatography-mass spectrometry.Results:These results showed that DOEO had significant myocardial cell protection,with IC50 values ranging from 17.64 to 24.78μg/mL in vitro.Compared to the myocardial ischemia group,the DOEO pretreatment groups had lower levels of myocardial injury,creatinine kinase,lactate dehydrogenase,alanine transaminase,aspartate transaminase,hydrogen peroxide,and nitric oxide,and higher levels of glutathione and superoxide dismutase.In addition,DOEO pretreatment significantly increased Na+-K+-ATPase and Ca2+-ATPase levels.Moreove r,immunohistochemical e xperiments showed that DOEO remarkably increased the protein levels of NF-E2-related nuclear factor 2(Nrf2)and heme oxygenase-1(HO-1)and reduced the expression of apoptotic caspases,including caspase 3 and caspase 9.The main components of the blood were transnerolidol and nerolidol oxide.Overall,the study showed that DOEO displayed myocardial protection by upregulating the NF-E2-related nuclear factor-antioxidant response element(Nrf2-ARE)and caspase pathways.DOEO has a therapeutic effect on MI by inhibiting the oxidant and apoptotic effects.Conclusions:D.odorifera may be a potential candidate drug for treating myocardial ischemic injury.
基金supported by National Natural Science Foundation of China(Nos.82100417,81760094,81760724)The Foundation of Jiangxi Provincial Department of Science and Technology Project(Nos.20202ACBL206001,20212BAB206022,20181BAB205026)+1 种基金Youth Project of Jiangxi Education Department(No.GJJ200217)Open Project of Key Laboratory of Modern of TCM,Ministry of Education Jiangxi University of Traditional Chinese Medicine(TCM-2019010).
文摘The attenuation function of Dalbergia odorifera leaves on cerebral ischemia-reperfusion(I/R)is little known.The candidate targets for the Chinese herb were extracted from brain tissues through the high-affinity chromatography.The molecular mechanism of D.odorifera leaves on cerebral I/R was investigated.Methods:Serial affinity chromatography based on D.odorifera leaves extract(DLE)affinity matrices were applied to find specific binding proteins in the brain tissues implemented on C57BL/6 mice by intraluminal middle cerebral artery occlusion for 1 h and reperfusion for 24 h.Specific binding proteins were subjected to mass-spectrometry to search for the differentially expressed proteins between control and DLE-affinity matrices.The hub genes were screened based on weighted gene co-expression network analysis(WGCNA).Then,predictive biology and potential experimental verification were performed for the candidate genes.The protective role of DLE in blood-brain barrier damage in cerebral I/R mice was evaluated by the leakage of Evans blue,western blotting,immunohistochemistry,and immunofluorescent staining.Results:952 differentially expressed proteins were classified into seven modules based on WGCNA under soft threshold 6.Based on WGCNA,AKT1,PIK3CA,NOS3,SMAD3,SMAD1,IL6,MAPK1,TGFBR2,TGFBR1,MAPK3,IGF1R,LRG1,mTOR,ROCK1,TGFB1,IL1B,SMAD2,and SMAD518 candidate hub proteins were involved in turquoise module.TGF-β,MAPK,focal adhesion,and adherens junction signaling pathway were associated with candidate hub proteins.Gene ontology analysis demonstrated that candidate hub proteins were related to the TGF-βreceptor signaling pathway,common-partner SMAD protein phosphorylation,etc.DLE could significantly reduce the leakage of Evans blue in mice with cerebral I/R,while attenuating the expression of occludin,claudin-5,and zonula occludens-1.Western blotting demonstrated that regulation of TGF-β/SMAD signaling pathway played an essential role in the protective effect of DLE.Conclusion:Thus,a number of candidate hub proteins were
基金Supported by the Applied Technology Research and Development Demonstration and Extension Project in Hainan Province(ZDXM2014101)
文摘Dalbergia odorifera is particularly scarce,and the D. odorifera furniture and commercial raw materials on the market are mainly old furniture and agricultural tools,as well as commercially collected D. odorifera logs. The logs and high-grade furniture made from D. odorifera are extremely expensive. It takes several decades for D. odorifera to grow into wood. At present,there is only a small area of newly planted and regenerated forest,and the planting area of D. odorifera is about 15 000 ha in Hainan Island,distributed in the low altitude area of Hainan Island. It is suggested that Hainan D. odorifera island should be built through the transformation of low-yielding forest land,the use of wasteland and marginal cultivated land,land around houses,green belt and roadside land for the cultivation of D. odorifera,and the development area can be up to 100 000-150 000 ha. It is necessary to mobilize farmers to revitalize the rural green economy,plant D. odorifera,beautify the environment,and increase the economic resources of rural trees; actively publicize the legal nature and obligation of D. odorifera protection,and create a strong atmosphere for each family to plant and protect D. odorifera.
文摘Tar spot of Dalbergia odorifera, caused by Phyllachora dalbergiicola, is a common disease, and se-riously affected the rate of photosynthesis. Here we developed a species-specific Nested-PCR approach for rapidand accurate detection of P. dalbergiicola, based on the differences in internal transcribed spacer （ITS） se-quences of P. dalbergiicola and another P. spp., an endophytic fungus, from which a pair of species-specificprimers P1/P2 （P1 ： 5＂-CGAGGTCAGAATCAAACG-3＂, P2： 5＂-TGAAGAACGCAGCGAAAT-3＂）, was designed.P1/P2 amplified only a unique 273 bp band from the genomic DNA of P. dalbergiicola. A Nested-PCR proce-dure using ITS4/ITS5 as the first-round primers, followed by P1/P2 primers, increased detection sensitivity10 000 fold to 100 ag. Using the Fast DNA-kit to extract DNA from the diseased plant tissues, the detection ofthe pathogen by Nested-PCR assay could be completed within I day. The results suggested that the PCR-basedmethods here could simplify both plant disease diagnosis and pathogen detection.
