围绕着胚囊,珠心组织中经常出现核物质的穿壁运动,并且以近合点端尤为频繁,穿壁核呈现明显的 ATP 酶活性。随着反足细胞的迅速增殖,内层珠心细胞原生质逐步解体,内含物以多种囊泡和无定形核物质为主要形式,大量迁入胚囊为反足细胞所利...围绕着胚囊,珠心组织中经常出现核物质的穿壁运动,并且以近合点端尤为频繁,穿壁核呈现明显的 ATP 酶活性。随着反足细胞的迅速增殖,内层珠心细胞原生质逐步解体,内含物以多种囊泡和无定形核物质为主要形式,大量迁入胚囊为反足细胞所利用。反足细胞胞壁封闭不完全,其原生质体的局部裸露以及胞间孔道的存留,极有利于珠心解体产物的摄入与转运,从而为反足细胞的加速增长提供了丰富的物质基础。对反足细胞增殖的几种可能方式,亦进行了讨论。展开更多
During the diversification of angiosperms, seeds have evolved structuralp chemical, molecular and physiolog- ically developing changes that specially affect the nucellus and endosperm. All through seed evolution, prog...During the diversification of angiosperms, seeds have evolved structuralp chemical, molecular and physiolog- ically developing changes that specially affect the nucellus and endosperm. All through seed evolution, programmed celldeath （PCD） has played a fundamental role. However, examples of PCD during seed development are limited. The present review examines PCD in integuments, nucellus, suspensor and endosperm in those representative examples of seeds studied to date.展开更多
Studies were initiated to explore the role of nucellus tissues and growth regulators in plantlets regeneration via somatic embryogenesis of Kinnow mandarin [Citrus reticulata L. (Blanco)]. Nucellus tissues were cultur...Studies were initiated to explore the role of nucellus tissues and growth regulators in plantlets regeneration via somatic embryogenesis of Kinnow mandarin [Citrus reticulata L. (Blanco)]. Nucellus tissues were cultured on MS medium supplemented with different concentrations and combinations of auxins, cytokinins and malt extract for primary callus induction. The best response for primary callus induction (90%) was obtained when MS medium was supplemented with 5 mg/l 2,4-D and 500 mg/l malt extract. Best results for embryogenic callus induction (80%) were obtained in C<sub>8</sub> medium. The induction of somatic embryos was highest when MS medium was supplemented with 1 mg/l BAP and maturation of somatic embryos occurred when MS medium was supplemented with 5 mg/l 2,4-D and 1 mg/l BAP. Maximum plantlets were regenerated (92%) from the somatic embryos on half strength MS medium with no hormones. The plantlets were successfully acclimatized in different potting mixtures and highest survival rate (100%) was achieved in potting mixture containing sand and peat moss (2:1).展开更多
[Objective] The research aimed to study a new whole clearing and preparation technology.[Method] After young seeds of Stephania sp.preserved in the alcohol solution,as well as the fresh pistil and nucellus of Sorghum ...[Objective] The research aimed to study a new whole clearing and preparation technology.[Method] After young seeds of Stephania sp.preserved in the alcohol solution,as well as the fresh pistil and nucellus of Sorghum bicolor were separated,they were soaked and cleared in the solution of 84 disinfectant.And the plant materials could be mounted by using three methods.[Result] The globular embryos and heart-shaped embryos at different stages of Stephania sp.as well as the pistil,nucellus,embryo sac,hypostase o...展开更多
文摘围绕着胚囊,珠心组织中经常出现核物质的穿壁运动,并且以近合点端尤为频繁,穿壁核呈现明显的 ATP 酶活性。随着反足细胞的迅速增殖,内层珠心细胞原生质逐步解体,内含物以多种囊泡和无定形核物质为主要形式,大量迁入胚囊为反足细胞所利用。反足细胞胞壁封闭不完全,其原生质体的局部裸露以及胞间孔道的存留,极有利于珠心解体产物的摄入与转运,从而为反足细胞的加速增长提供了丰富的物质基础。对反足细胞增殖的几种可能方式,亦进行了讨论。
文摘During the diversification of angiosperms, seeds have evolved structuralp chemical, molecular and physiolog- ically developing changes that specially affect the nucellus and endosperm. All through seed evolution, programmed celldeath （PCD） has played a fundamental role. However, examples of PCD during seed development are limited. The present review examines PCD in integuments, nucellus, suspensor and endosperm in those representative examples of seeds studied to date.
文摘Studies were initiated to explore the role of nucellus tissues and growth regulators in plantlets regeneration via somatic embryogenesis of Kinnow mandarin [Citrus reticulata L. (Blanco)]. Nucellus tissues were cultured on MS medium supplemented with different concentrations and combinations of auxins, cytokinins and malt extract for primary callus induction. The best response for primary callus induction (90%) was obtained when MS medium was supplemented with 5 mg/l 2,4-D and 500 mg/l malt extract. Best results for embryogenic callus induction (80%) were obtained in C<sub>8</sub> medium. The induction of somatic embryos was highest when MS medium was supplemented with 1 mg/l BAP and maturation of somatic embryos occurred when MS medium was supplemented with 5 mg/l 2,4-D and 1 mg/l BAP. Maximum plantlets were regenerated (92%) from the somatic embryos on half strength MS medium with no hormones. The plantlets were successfully acclimatized in different potting mixtures and highest survival rate (100%) was achieved in potting mixture containing sand and peat moss (2:1).
基金Supported by National Natural Science Foundation of China(30770124)~~
文摘[Objective] The research aimed to study a new whole clearing and preparation technology.[Method] After young seeds of Stephania sp.preserved in the alcohol solution,as well as the fresh pistil and nucellus of Sorghum bicolor were separated,they were soaked and cleared in the solution of 84 disinfectant.And the plant materials could be mounted by using three methods.[Result] The globular embryos and heart-shaped embryos at different stages of Stephania sp.as well as the pistil,nucellus,embryo sac,hypostase o...
