Objective To investigate the effects of YOD1 overexpression on the proliferation and migration of human oral keratinocytes（HOKs）, and to clarify whether the mechanisms involve transforming growth factor-β（TGF-β）...Objective To investigate the effects of YOD1 overexpression on the proliferation and migration of human oral keratinocytes（HOKs）, and to clarify whether the mechanisms involve transforming growth factor-β（TGF-β） signaling. Methods HOKs were transfected with the plasmid p EGFP-N3-YOD1 containing YOD1. The mR NA levels of YOD1 and TGF-β were determined by q PCR. The protein expressions of YOD1, TGF-β, Smad2/3, Smad4, and phospho-Smad2/3 were determined by western blotting. Cell proliferation and migration were evaluated by Cell Counting Kit-8 assay and wound healing assay, respectively. Results The m RNA and protein levels of YOD1 were higher in HOKs transfected with YOD1. YOD1 overexpression significantly enhanced the migration of HOKs. The mR NA and protein levels of TGF-β3 were increased by YOD1 overexpression. HOKs transfected with YOD1 exhibited increased phospho-Smad2/3 levels. Conclusion YOD1 overexpression enhances cell migration by promoting TGF-β3 signaling which may play an important role in lip and palate formation. YOD1 mutation may contribute to aberrant TGF-β3 signaling associated with decreased cell migration resulting in NSCLP.展开更多
目的对非综合征唇腭裂患者与骨性Ⅰ类人群的上气道对称性进行三维分析对比研究。方法选择非综合征唇腭裂患者51例(男性37例,女性14例,平均年龄(17.40±5.93)岁)和骨性Ⅰ类人群53例(男39例,女14例,平均年龄(17.40±6.07)岁),拍...目的对非综合征唇腭裂患者与骨性Ⅰ类人群的上气道对称性进行三维分析对比研究。方法选择非综合征唇腭裂患者51例(男性37例,女性14例,平均年龄(17.40±5.93)岁)和骨性Ⅰ类人群53例(男39例,女14例,平均年龄(17.40±6.07)岁),拍摄锥形束CT,应用Dolphin 3D 11.9软件进行三维测量分析,采用SPSS 25.0软件包进行独立样本t检验对比两者上气道对称性。结果单侧唇腭裂患者上气道鼻咽段和腭咽段为不对称结构(P<0.05),其患侧横截面直径小于健侧;而双侧唇腭裂患者及骨性Ⅰ类人群,其上气道鼻咽和腭咽段为基本对称结构(P>0.05)。结论单侧唇腭裂患者的上气道横截面直径中,有患侧偏向健侧的趋势,应注重其患侧的通气及呼吸情况。展开更多
[目的]系统评价腹前同源盒1(VAX1)基因多态性与非综合征性唇腭裂易感性的关联。[方法]检索PubMed、EMbase、Web of Science和中国知网数据库,检索时限均为建库到2020年3月31日,共检索到126篇文献。应用RevMan 5.3与STATA 15.0对所有提...[目的]系统评价腹前同源盒1(VAX1)基因多态性与非综合征性唇腭裂易感性的关联。[方法]检索PubMed、EMbase、Web of Science和中国知网数据库,检索时限均为建库到2020年3月31日,共检索到126篇文献。应用RevMan 5.3与STATA 15.0对所有提取到的数据进行分析。[结果]在进行文献筛选后,共纳入13篇相关文献,其中试验组3963例,对照组2557例。分析结果显示:VAX1基因rs7078160位点[A vs G:OR=1.33,95%CI(1.17,1.51),P<0.001;AG vs GG:OR=1.29,95%CI(1.15,1.46),P<0.001;AA vs GG:OR=1.67,95%CI(1.23,2.28),P=0.001;AA vs AG+GG:OR=1.52,95%CI(1.16,2.01),P=0.003;AA+AG vs GG:OR=1.37,95%CI(1.22,1.53),P<0.001]与rs4752028位点[C vs T:OR=1.60,95%CI(1.20,2.14),P=0.001;CC vs TT:OR=2.65,95%CI(1.46,4.81),P=0.001;CC vs CT+TT:OR=2.34,95%CI(1.32,4.14),P=0.004;CC+CT vs TT:OR=1.62,95%CI(1.10,2.38),P=0.014]是非综合征性唇腭裂的易感因素。[结论]VAX1基因rs7078160位点多态性与非综合征性唇腭裂有关,A位点是危险因素;VAX1基因rs4752028位点多态性与非综合征性唇腭裂可能有关,C位点是危险因素。展开更多
基金supported by National Natural Science Foundations of China[No.81273103]the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
文摘Objective To investigate the effects of YOD1 overexpression on the proliferation and migration of human oral keratinocytes（HOKs）, and to clarify whether the mechanisms involve transforming growth factor-β（TGF-β） signaling. Methods HOKs were transfected with the plasmid p EGFP-N3-YOD1 containing YOD1. The mR NA levels of YOD1 and TGF-β were determined by q PCR. The protein expressions of YOD1, TGF-β, Smad2/3, Smad4, and phospho-Smad2/3 were determined by western blotting. Cell proliferation and migration were evaluated by Cell Counting Kit-8 assay and wound healing assay, respectively. Results The m RNA and protein levels of YOD1 were higher in HOKs transfected with YOD1. YOD1 overexpression significantly enhanced the migration of HOKs. The mR NA and protein levels of TGF-β3 were increased by YOD1 overexpression. HOKs transfected with YOD1 exhibited increased phospho-Smad2/3 levels. Conclusion YOD1 overexpression enhances cell migration by promoting TGF-β3 signaling which may play an important role in lip and palate formation. YOD1 mutation may contribute to aberrant TGF-β3 signaling associated with decreased cell migration resulting in NSCLP.
文摘目的对非综合征唇腭裂患者与骨性Ⅰ类人群的上气道对称性进行三维分析对比研究。方法选择非综合征唇腭裂患者51例(男性37例,女性14例,平均年龄(17.40±5.93)岁)和骨性Ⅰ类人群53例(男39例,女14例,平均年龄(17.40±6.07)岁),拍摄锥形束CT,应用Dolphin 3D 11.9软件进行三维测量分析,采用SPSS 25.0软件包进行独立样本t检验对比两者上气道对称性。结果单侧唇腭裂患者上气道鼻咽段和腭咽段为不对称结构(P<0.05),其患侧横截面直径小于健侧;而双侧唇腭裂患者及骨性Ⅰ类人群,其上气道鼻咽和腭咽段为基本对称结构(P>0.05)。结论单侧唇腭裂患者的上气道横截面直径中,有患侧偏向健侧的趋势,应注重其患侧的通气及呼吸情况。
文摘[目的]系统评价腹前同源盒1(VAX1)基因多态性与非综合征性唇腭裂易感性的关联。[方法]检索PubMed、EMbase、Web of Science和中国知网数据库,检索时限均为建库到2020年3月31日,共检索到126篇文献。应用RevMan 5.3与STATA 15.0对所有提取到的数据进行分析。[结果]在进行文献筛选后,共纳入13篇相关文献,其中试验组3963例,对照组2557例。分析结果显示:VAX1基因rs7078160位点[A vs G:OR=1.33,95%CI(1.17,1.51),P<0.001;AG vs GG:OR=1.29,95%CI(1.15,1.46),P<0.001;AA vs GG:OR=1.67,95%CI(1.23,2.28),P=0.001;AA vs AG+GG:OR=1.52,95%CI(1.16,2.01),P=0.003;AA+AG vs GG:OR=1.37,95%CI(1.22,1.53),P<0.001]与rs4752028位点[C vs T:OR=1.60,95%CI(1.20,2.14),P=0.001;CC vs TT:OR=2.65,95%CI(1.46,4.81),P=0.001;CC vs CT+TT:OR=2.34,95%CI(1.32,4.14),P=0.004;CC+CT vs TT:OR=1.62,95%CI(1.10,2.38),P=0.014]是非综合征性唇腭裂的易感因素。[结论]VAX1基因rs7078160位点多态性与非综合征性唇腭裂有关,A位点是危险因素;VAX1基因rs4752028位点多态性与非综合征性唇腭裂可能有关,C位点是危险因素。
