Cigar line Beinhart 1000-1 has effective durable resistance to black shank(BS) and is considered one of the most resistant sources in tobacco(Nicotiana tabacum L.). To investigate the inheritance and identification of...Cigar line Beinhart 1000-1 has effective durable resistance to black shank(BS) and is considered one of the most resistant sources in tobacco(Nicotiana tabacum L.). To investigate the inheritance and identification of stable quantitative trait loci(QTL) for BS response, F2,BC1 F2 individuals and BC1 F2:3 lines were produced from a cross between Beinhart 1000-1 and Xiaohuangjin 1025. Two major quantitative trait loci(M-QTL) named qBS7 and qBS17 were repeatedly detected under different conditions. QTL qBS7 was mapped to the region between PT30174 and PT60621 and explained 17.40%–25.60% of the phenotypic variance under different conditions. The other QTL qBS17 in interval PT61564–PT61538 of linkage group 17 was detected in a BC1 F2 population in the field and in BC1 F2:3 in both the field and at the seedling stage, explaining 6.90% to 11.60% of the phenotypic variance. The results improve our understanding of the inheritance of resistance to BS and provide information that can be used in marker-assisted breeding.展开更多
The degradation of tilmicosin(TLM),a semi-synthetic 16-membered macrolide antibiotic,has been receiving increasing attention.Conventionally,there are three tilmicosin degradation methods,and among them microbial degra...The degradation of tilmicosin(TLM),a semi-synthetic 16-membered macrolide antibiotic,has been receiving increasing attention.Conventionally,there are three tilmicosin degradation methods,and among them microbial degradation is considered the best due to its high efficiency,eco-friendliness,and low cost.Coincidently,we found a new strain,Glutamicibacter nicotianae sp.AT6,capable of degrading high-concentration TLM at 100 mg/L with a 97%removal efficiency.The role of tryptone was as well investigated,and the results revealed that the loading of tryptone had a significant influence on TLM removals.The toxicity assessment indicated that strain AT6 could efficiently convert TLM into less-toxic substances.Based on the identified intermediates,the degradation of TLM by AT6 processing through two distinct pathways was then proposed.展开更多
Crop rotations are widely used because they can significantly reduce the incidence of pests and diseases.The interactions between non-host roots and pathogens may be key in the inhibition of soilborne pathogens in cro...Crop rotations are widely used because they can significantly reduce the incidence of pests and diseases.The interactions between non-host roots and pathogens may be key in the inhibition of soilborne pathogens in crop rotations.Interactions between fennel(Foeniculum vulgare)roots/root exudates and Phytophthora nicotianae were investigated because of the known allelopathy between fennel and tobacco(Nicotiana tabacum).The effects of the key compounds in the fennel rhizosphere on the mycelial growth and zoospore behavior of P.nicotianae were assessed.The roots of fennel attracted P.nicotianae zoospores and inhibited their motility and the germination of cystospores,with some cystospores rupturing.4-ethylacetophenone,vanillin and N-formylpiperidine were consistently identified in the fennel rhizosphere and were found to interfere with the infection of P.nicotianae,especially vanillin.Hyphae treated with these compounds produced more abnormal branches and accumulated reactive oxygen species.These interspecific interactions between non-host roots and pathogens were found to be an important factor in the inhibition by fennel of infection by P.nicotianae.展开更多
Phytophthora nicotianae causes substantial economic losses in most countries where tobacco is produced.At present,the control of P.nicotianae mainly depends on chemical methods,with considerable environmental and heal...Phytophthora nicotianae causes substantial economic losses in most countries where tobacco is produced.At present,the control of P.nicotianae mainly depends on chemical methods,with considerable environmental and health issues.We investigated the effects of ethanol extracts from Scutellaria baicalensis Georgi(SBG)and Magnolia officinalis(MO).On mycelial growth,sporangium formation,and zoospore release of P.nicotianae.Both extracts inhibited the growth of P.nicotianae,with mycelial growth inhibition rates of 88.92%and 93.92%,respectively,at 40 mg/mL,and EC50 values of 5.39 and 5.74 mg/mL,respectively.The underlying mechanisms were the inhibition of sporangium formation,the reduction of zoospore number,and the destruction of the mycelium structure.At an SBG extract concentration of 16.17 mg/mL,the inhibition rates for sporangia and zoospores were 98.66%and 99.39%,respectively.At an MO extract concentration of 2.87 mg/mL,the production of sporangia and zoospores was completely inhibited.The hyphae treated with the two plant extracts showed different degrees of deformation and damage.Hyphae treated with SBG extract showed adhesion and local swelling,whereas treatment with MO extract resulted in broken hyphae.Mixture of the extracts resulted in a good synergistic effect.展开更多
Objective To control stem blight disease of Schizonepeta tenuifolia caused by Phytophthora nicotianae. Methods The antagonist effect of 13 Trichoderma strains (including T. viride and T. harzianum) was evaluated upon ...Objective To control stem blight disease of Schizonepeta tenuifolia caused by Phytophthora nicotianae. Methods The antagonist effect of 13 Trichoderma strains (including T. viride and T. harzianum) was evaluated upon mycelia growth of P. nicotianae. Trichoderma strains with high antagonistic activities against the pathogen were used to control stem blight of S. tenuifolia in the field. Results Of 13 Trichoderma strains tested, T. viride strain M3 showed maximum mycelia growth inhibition (83.2%) to the pathogen, followed by T. viride strain Tv04-2 (78.2%) and then T. harzianum strain ThB (65.0%), in vitro. Fungal cell wall degrading enzymes, protease, and β-1,3-glucanase were analyzed qualitatively and quantitatively in further study. T. viride strains M3, Tv04-2, and T. harzianum strain ThB efficiently against P. nicotianae were used to control stem blight of S. tenuifolia in the field, and T. viride strain M3 showed the best biocontrol potential. Conclusion Trichoderma spp. can be used as alternatives of pesticides to control stem blight, one of the serious soilborne diseases of S. tenuifolia caused by P. nicotianae. However, though T. viride strains Tv04-2 and T. harzianum strain ThB are also highly against P. nicotianae in vitro, the controlling efficacy of them on stem blight disease is not as excellent as T. viride strains M3 in the field.展开更多
[Objective] This study aimed to investigate the resistance to different fungicides in Phytophthora parasitica var. nicotianae. [Method] Under indoor incubation conditions, the resistance to dimethomorph, metalaxyl-man...[Objective] This study aimed to investigate the resistance to different fungicides in Phytophthora parasitica var. nicotianae. [Method] Under indoor incubation conditions, the resistance to dimethomorph, metalaxyl-mancozeb, propamocarb and ovraclostrobin.dimethomorph in P. parasitica strain isolated from Zhenyuan County in Qiandongnan State was analyzed with colony growth measurement method. [Result] P. parasitica exhibited different levels of sensitivity to four fungicides. To be specific, P. parasitica exhibited the highest resistance to dimethomorph, and ECho reached 1.19 μg/ml. [Conclusion] In Zhenyuan tobacco-growing area, long-term single use of dimethomorph possesses certain resistance risk in prevention and control of black shank disease.展开更多
[ Objective] To overcome the resistance of Phytophthora parasitica var. nicotianae against metalaxyl and effectively control its damage, new efficient complex agent of metalaxyl was studied and developed. [ Method] Th...[ Objective] To overcome the resistance of Phytophthora parasitica var. nicotianae against metalaxyl and effectively control its damage, new efficient complex agent of metalaxyl was studied and developed. [ Method] The toxieities of nine fungicides against P. parasitica were measured using growth rate method. On this basis, the fungicides with good effects were selected to compound with metalaxyl, and the optimum complex ratio was confirmed. [Result] The toxicity of metalaxyl was the strongest with EC50 of 2. 130 5μg/ml; followed by carbendazim, mancezeb and dimethomorph with EC50 of 2.357 9, 2.639 8 and 2. 778 8 μg/ml. The effect of cyazofamid was the poorest with EC50 of 6. 278 8 μg/ml. The optimum complex ratios of dimethomorph, carbendazim and mancezeb with metalaxyl were 40： 60, 30：70 and 20： 80, and their co-toxicity coefficients were 138.80,124.25 and 115.00, respectively. [ Conclusion] The complex agents had application and promotion value, which could be used to carry out further field trials.展开更多
The removal of Cr(III) from aqueous Cr(III) using Arthrobacter nicotianae cells was examined. Cr(III) removal was strongly affected by the pH of the solution and the amounts of Cr(III) removed increased as the pH (1 -...The removal of Cr(III) from aqueous Cr(III) using Arthrobacter nicotianae cells was examined. Cr(III) removal was strongly affected by the pH of the solution and the amounts of Cr(III) removed increased as the pH (1 - 5) of the solution increased. The removal of Cr(III) using the cells was also strongly affected by the Cr(III) concentration of the solution, and obeyed the Langmuir isotherm. The percentage of Cr increased as the cell quantity increased, whereas the amount of Cr (μmol/g dry wt. cells) decreased. The removal of Cr(III) using the cells was very fast, and reached an equilibrium within 6 h from the supply of Cr(III) in the solution. A small amount of Cr(III) absorbed by immobilized cells was desorbed at 30oC;however, most was desorbed at reflux temperature using diluted HCl. Cr(III) adsorption-desorption cycles can be repeated 5 times using immobilized cells. These results have practical implications for industrial wastewater management.展开更多
Tobacco black shank caused by Phytophthora parasitica var.nicotianae(Breda de Hean)is one of the most serious diseases in tobacco growing,and few effective fungicides are available currently.The purpose of this study ...Tobacco black shank caused by Phytophthora parasitica var.nicotianae(Breda de Hean)is one of the most serious diseases in tobacco growing,and few effective fungicides are available currently.The purpose of this study was to assess the feasibility of using fluazinam to treat tobacco black shank disease.In vitro toxicities of fluazinam to 62 isolates of P.nicotianae were determined by mycelial growth rate method.The EC50 values ranged from 0.294 6 to 2.389 9 μg/mL with a mean of 1.084 0 μg/mL.The sensitivity distribution was unimodal,and there was no resistant isolate in P.nicotianae population.The carboxylic acid amide(CAA)fungicides were single-site in action and activity to oomycetes.The synergistic interactions were tested by Wadley method between fluazinam and dimethomorph,flumorph,mandipropamid,respectively.The mixture containing dimethomoph:Huazinam at 1:2 ratio showed additive actions,with the synergy ratio of 1.2.The mixture containing flumorph and fluazinam at 1:5 ratio had the highest synergy ratio(SR 2.07).Mandipropamid and fluazinam had an additive interaction and the proportion of 2:1 had the lowest EC50 value(0.020 7 μg/mL).This study demonstrated the sensitivity of P.nicotianae to fluazinam,and provided the experimental foundation to use fluazinam for tobacco black shank management.展开更多
基金supported by grants from the Agricultural Science and Technology Innovation Program (ASTIP-TRIC01)National Natural Science Foundation of China (31571738)
文摘Cigar line Beinhart 1000-1 has effective durable resistance to black shank(BS) and is considered one of the most resistant sources in tobacco(Nicotiana tabacum L.). To investigate the inheritance and identification of stable quantitative trait loci(QTL) for BS response, F2,BC1 F2 individuals and BC1 F2:3 lines were produced from a cross between Beinhart 1000-1 and Xiaohuangjin 1025. Two major quantitative trait loci(M-QTL) named qBS7 and qBS17 were repeatedly detected under different conditions. QTL qBS7 was mapped to the region between PT30174 and PT60621 and explained 17.40%–25.60% of the phenotypic variance under different conditions. The other QTL qBS17 in interval PT61564–PT61538 of linkage group 17 was detected in a BC1 F2 population in the field and in BC1 F2:3 in both the field and at the seedling stage, explaining 6.90% to 11.60% of the phenotypic variance. The results improve our understanding of the inheritance of resistance to BS and provide information that can be used in marker-assisted breeding.
基金supported by the National Natural Science Foundation of China(Nos.21868011,42106144,42077444)the National Key R&D Program of China(No.2017YFC1103800)the financial support from Shandong University of Science and Technology(No.SKR19-1-012)。
文摘The degradation of tilmicosin(TLM),a semi-synthetic 16-membered macrolide antibiotic,has been receiving increasing attention.Conventionally,there are three tilmicosin degradation methods,and among them microbial degradation is considered the best due to its high efficiency,eco-friendliness,and low cost.Coincidently,we found a new strain,Glutamicibacter nicotianae sp.AT6,capable of degrading high-concentration TLM at 100 mg/L with a 97%removal efficiency.The role of tryptone was as well investigated,and the results revealed that the loading of tryptone had a significant influence on TLM removals.The toxicity assessment indicated that strain AT6 could efficiently convert TLM into less-toxic substances.Based on the identified intermediates,the degradation of TLM by AT6 processing through two distinct pathways was then proposed.
基金This study was supported by the National Natural Science Foundation of China(31972328,31601682)the Natural Science Foundation of Yunnan Province(2018FG001-046)+3 种基金the Major Program in Yunnan Province(202002AE320012-02-03)the key Yunnan provincial programs of Yunnan Eco-Friendly Food International Cooperation Research Center(2019ZG00901-03)the Yunnan Academician Workstation of Chinese Academy of Engineering(2018IC063)the Program for Innovative Research Team in Science and Technology in University of Yunnan Province.
文摘Crop rotations are widely used because they can significantly reduce the incidence of pests and diseases.The interactions between non-host roots and pathogens may be key in the inhibition of soilborne pathogens in crop rotations.Interactions between fennel(Foeniculum vulgare)roots/root exudates and Phytophthora nicotianae were investigated because of the known allelopathy between fennel and tobacco(Nicotiana tabacum).The effects of the key compounds in the fennel rhizosphere on the mycelial growth and zoospore behavior of P.nicotianae were assessed.The roots of fennel attracted P.nicotianae zoospores and inhibited their motility and the germination of cystospores,with some cystospores rupturing.4-ethylacetophenone,vanillin and N-formylpiperidine were consistently identified in the fennel rhizosphere and were found to interfere with the infection of P.nicotianae,especially vanillin.Hyphae treated with these compounds produced more abnormal branches and accumulated reactive oxygen species.These interspecific interactions between non-host roots and pathogens were found to be an important factor in the inhibition by fennel of infection by P.nicotianae.
基金funded by financial grants from the Education Department of Hunan Province(SCX1840 and CX20190515).
文摘Phytophthora nicotianae causes substantial economic losses in most countries where tobacco is produced.At present,the control of P.nicotianae mainly depends on chemical methods,with considerable environmental and health issues.We investigated the effects of ethanol extracts from Scutellaria baicalensis Georgi(SBG)and Magnolia officinalis(MO).On mycelial growth,sporangium formation,and zoospore release of P.nicotianae.Both extracts inhibited the growth of P.nicotianae,with mycelial growth inhibition rates of 88.92%and 93.92%,respectively,at 40 mg/mL,and EC50 values of 5.39 and 5.74 mg/mL,respectively.The underlying mechanisms were the inhibition of sporangium formation,the reduction of zoospore number,and the destruction of the mycelium structure.At an SBG extract concentration of 16.17 mg/mL,the inhibition rates for sporangia and zoospores were 98.66%and 99.39%,respectively.At an MO extract concentration of 2.87 mg/mL,the production of sporangia and zoospores was completely inhibited.The hyphae treated with the two plant extracts showed different degrees of deformation and damage.Hyphae treated with SBG extract showed adhesion and local swelling,whereas treatment with MO extract resulted in broken hyphae.Mixture of the extracts resulted in a good synergistic effect.
基金Central Public-interest Scientific Institution Basal Research Fund (yz-08-09)
文摘Objective To control stem blight disease of Schizonepeta tenuifolia caused by Phytophthora nicotianae. Methods The antagonist effect of 13 Trichoderma strains (including T. viride and T. harzianum) was evaluated upon mycelia growth of P. nicotianae. Trichoderma strains with high antagonistic activities against the pathogen were used to control stem blight of S. tenuifolia in the field. Results Of 13 Trichoderma strains tested, T. viride strain M3 showed maximum mycelia growth inhibition (83.2%) to the pathogen, followed by T. viride strain Tv04-2 (78.2%) and then T. harzianum strain ThB (65.0%), in vitro. Fungal cell wall degrading enzymes, protease, and β-1,3-glucanase were analyzed qualitatively and quantitatively in further study. T. viride strains M3, Tv04-2, and T. harzianum strain ThB efficiently against P. nicotianae were used to control stem blight of S. tenuifolia in the field, and T. viride strain M3 showed the best biocontrol potential. Conclusion Trichoderma spp. can be used as alternatives of pesticides to control stem blight, one of the serious soilborne diseases of S. tenuifolia caused by P. nicotianae. However, though T. viride strains Tv04-2 and T. harzianum strain ThB are also highly against P. nicotianae in vitro, the controlling efficacy of them on stem blight disease is not as excellent as T. viride strains M3 in the field.
文摘[Objective] This study aimed to investigate the resistance to different fungicides in Phytophthora parasitica var. nicotianae. [Method] Under indoor incubation conditions, the resistance to dimethomorph, metalaxyl-mancozeb, propamocarb and ovraclostrobin.dimethomorph in P. parasitica strain isolated from Zhenyuan County in Qiandongnan State was analyzed with colony growth measurement method. [Result] P. parasitica exhibited different levels of sensitivity to four fungicides. To be specific, P. parasitica exhibited the highest resistance to dimethomorph, and ECho reached 1.19 μg/ml. [Conclusion] In Zhenyuan tobacco-growing area, long-term single use of dimethomorph possesses certain resistance risk in prevention and control of black shank disease.
基金Supported by Key Project of Sichuan Education Department "Study on Physiological Race of Tobacco Black Shank in Liangshan and Resistance Evaluation of Tobacco Germplasm Resources in Sichuan Province"(08ZA032)~~
文摘[ Objective] To overcome the resistance of Phytophthora parasitica var. nicotianae against metalaxyl and effectively control its damage, new efficient complex agent of metalaxyl was studied and developed. [ Method] The toxieities of nine fungicides against P. parasitica were measured using growth rate method. On this basis, the fungicides with good effects were selected to compound with metalaxyl, and the optimum complex ratio was confirmed. [Result] The toxicity of metalaxyl was the strongest with EC50 of 2. 130 5μg/ml; followed by carbendazim, mancezeb and dimethomorph with EC50 of 2.357 9, 2.639 8 and 2. 778 8 μg/ml. The effect of cyazofamid was the poorest with EC50 of 6. 278 8 μg/ml. The optimum complex ratios of dimethomorph, carbendazim and mancezeb with metalaxyl were 40： 60, 30：70 and 20： 80, and their co-toxicity coefficients were 138.80,124.25 and 115.00, respectively. [ Conclusion] The complex agents had application and promotion value, which could be used to carry out further field trials.
文摘The removal of Cr(III) from aqueous Cr(III) using Arthrobacter nicotianae cells was examined. Cr(III) removal was strongly affected by the pH of the solution and the amounts of Cr(III) removed increased as the pH (1 - 5) of the solution increased. The removal of Cr(III) using the cells was also strongly affected by the Cr(III) concentration of the solution, and obeyed the Langmuir isotherm. The percentage of Cr increased as the cell quantity increased, whereas the amount of Cr (μmol/g dry wt. cells) decreased. The removal of Cr(III) using the cells was very fast, and reached an equilibrium within 6 h from the supply of Cr(III) in the solution. A small amount of Cr(III) absorbed by immobilized cells was desorbed at 30oC;however, most was desorbed at reflux temperature using diluted HCl. Cr(III) adsorption-desorption cycles can be repeated 5 times using immobilized cells. These results have practical implications for industrial wastewater management.
文摘Tobacco black shank caused by Phytophthora parasitica var.nicotianae(Breda de Hean)is one of the most serious diseases in tobacco growing,and few effective fungicides are available currently.The purpose of this study was to assess the feasibility of using fluazinam to treat tobacco black shank disease.In vitro toxicities of fluazinam to 62 isolates of P.nicotianae were determined by mycelial growth rate method.The EC50 values ranged from 0.294 6 to 2.389 9 μg/mL with a mean of 1.084 0 μg/mL.The sensitivity distribution was unimodal,and there was no resistant isolate in P.nicotianae population.The carboxylic acid amide(CAA)fungicides were single-site in action and activity to oomycetes.The synergistic interactions were tested by Wadley method between fluazinam and dimethomorph,flumorph,mandipropamid,respectively.The mixture containing dimethomoph:Huazinam at 1:2 ratio showed additive actions,with the synergy ratio of 1.2.The mixture containing flumorph and fluazinam at 1:5 ratio had the highest synergy ratio(SR 2.07).Mandipropamid and fluazinam had an additive interaction and the proportion of 2:1 had the lowest EC50 value(0.020 7 μg/mL).This study demonstrated the sensitivity of P.nicotianae to fluazinam,and provided the experimental foundation to use fluazinam for tobacco black shank management.
