BACKGROUND： Recent studies have paid much attention to the newly found neuregulin-1, which might be closely linked to the molecular genetics of schizophrenia. OBJECTIVE： To investigate the association of neuregulin-...BACKGROUND： Recent studies have paid much attention to the newly found neuregulin-1, which might be closely linked to the molecular genetics of schizophrenia. OBJECTIVE： To investigate the association of neuregulin-related genes with schizophrenia, and to summarize the advancements in this current research. RETRIEVAL STRATEGY： Using the terms ＂neuregulins, gene, schizophrenia＂, we retrieved articles published from January 2000 to June 2007 from http：//www.ncbi.nlm.gov, http：//www.elsevier.lib.tsinghua.edu.cn, and http：//www.cjfd.cnki.net to identify studies addressing the association of neuregulin-related genes to schizophrenia. At the same time, we searched more than 10 medical journals by hand. The languages were limited to English and Chinese. Forty-two manuscripts were obtained and were firstly screened. Inclusion criteria： studies on neuregulins, schizophrenia, neuregulin-1, and the pathogenesis of schizophrenia, including randomized, blinded, and other original studies. Exclusion criteria： studies not related to schizophrenia, or repetitive studies. LITERATURE EVALUATION： The included 42 manuscripts were sorted. Twenty-one were selected as references for this article： fourteen were basic studies, and the remaining articles were case-controlled studies or other. DATA SYNTHESIS： Neuregulins are primarily expressed in the nervous system and heart, and limited expression is also seen in other tissues.. These proteins transmit signals among certain cells and play an important role in normal development of the nervous system. Neuregulin-1 is a typical neuregulin-related gene. Neuregulin genes are closely related to glutamatergic, GABAergic, and dopaminergic neurons. CONCLUSION： Neuregulin-related genes, such as neuregulin-1, are important and promising candidate genes for studying schizophrenia disease. Their roles in the onset of schizophrenia, neuregulin-related gene expression products, and correlations of ErbB receptor to schizophrenia symptoms need to be further investigated. Furt展开更多
Objective： To explore the effects of neuregulins on ErbB2 receptor signal transduction pathway activation, and invasion and metastasis of non-overexpression ErbB2 breast cancer cell MDA-MB-231. Methods： The express...Objective： To explore the effects of neuregulins on ErbB2 receptor signal transduction pathway activation, and invasion and metastasis of non-overexpression ErbB2 breast cancer cell MDA-MB-231. Methods： The expressions of neuregulin were detected by immunocytochemistry and Western blot. MDA-MB-231 cells were treated with ErbB2 kinase inhibitor AG825. Proliferations were measured with MTT assay. Invasion and metastasis of MDA-ME-231 cells were evaluated with transwell chamber. The enzyme activities of MMP-2 and MMP-9 were detected by gelatin zymography. The expressions of MMP-2 and HIF-1α were detected by Western blot. Results： MDA-MB-231 cells expressed a relatively higher level of neuregulin. In Western blot, the positive reaction band was found at 44KD which coincides with the molecular weight of NRG. When MDA-MB-231 cells were treated with AG825, the proliferation was inhibited in a time-dose-dependent manner （P〈0.01）, invasion and metastasis were also depressed （P〈0.05）. The enzyme activities of MMP-2 and MMP-9 were lower （P〈0.05）. The expression levels of MMP-2 and HIF-lct were decreased （P〈0.05）. Conclusion： Our study indicates that neuregulins are synthesized in MDA-MB-231 cells as transmembrane proteins, neuregulins could activate ErbB2 receptor signal transduction pathway by autocrine or paracrine secretion, and induce invasion and metastasis of MDA-MB-231 cells.展开更多
文摘BACKGROUND： Recent studies have paid much attention to the newly found neuregulin-1, which might be closely linked to the molecular genetics of schizophrenia. OBJECTIVE： To investigate the association of neuregulin-related genes with schizophrenia, and to summarize the advancements in this current research. RETRIEVAL STRATEGY： Using the terms ＂neuregulins, gene, schizophrenia＂, we retrieved articles published from January 2000 to June 2007 from http：//www.ncbi.nlm.gov, http：//www.elsevier.lib.tsinghua.edu.cn, and http：//www.cjfd.cnki.net to identify studies addressing the association of neuregulin-related genes to schizophrenia. At the same time, we searched more than 10 medical journals by hand. The languages were limited to English and Chinese. Forty-two manuscripts were obtained and were firstly screened. Inclusion criteria： studies on neuregulins, schizophrenia, neuregulin-1, and the pathogenesis of schizophrenia, including randomized, blinded, and other original studies. Exclusion criteria： studies not related to schizophrenia, or repetitive studies. LITERATURE EVALUATION： The included 42 manuscripts were sorted. Twenty-one were selected as references for this article： fourteen were basic studies, and the remaining articles were case-controlled studies or other. DATA SYNTHESIS： Neuregulins are primarily expressed in the nervous system and heart, and limited expression is also seen in other tissues.. These proteins transmit signals among certain cells and play an important role in normal development of the nervous system. Neuregulin-1 is a typical neuregulin-related gene. Neuregulin genes are closely related to glutamatergic, GABAergic, and dopaminergic neurons. CONCLUSION： Neuregulin-related genes, such as neuregulin-1, are important and promising candidate genes for studying schizophrenia disease. Their roles in the onset of schizophrenia, neuregulin-related gene expression products, and correlations of ErbB receptor to schizophrenia symptoms need to be further investigated. Furt
文摘Objective： To explore the effects of neuregulins on ErbB2 receptor signal transduction pathway activation, and invasion and metastasis of non-overexpression ErbB2 breast cancer cell MDA-MB-231. Methods： The expressions of neuregulin were detected by immunocytochemistry and Western blot. MDA-MB-231 cells were treated with ErbB2 kinase inhibitor AG825. Proliferations were measured with MTT assay. Invasion and metastasis of MDA-ME-231 cells were evaluated with transwell chamber. The enzyme activities of MMP-2 and MMP-9 were detected by gelatin zymography. The expressions of MMP-2 and HIF-1α were detected by Western blot. Results： MDA-MB-231 cells expressed a relatively higher level of neuregulin. In Western blot, the positive reaction band was found at 44KD which coincides with the molecular weight of NRG. When MDA-MB-231 cells were treated with AG825, the proliferation was inhibited in a time-dose-dependent manner （P〈0.01）, invasion and metastasis were also depressed （P〈0.05）. The enzyme activities of MMP-2 and MMP-9 were lower （P〈0.05）. The expression levels of MMP-2 and HIF-lct were decreased （P〈0.05）. Conclusion： Our study indicates that neuregulins are synthesized in MDA-MB-231 cells as transmembrane proteins, neuregulins could activate ErbB2 receptor signal transduction pathway by autocrine or paracrine secretion, and induce invasion and metastasis of MDA-MB-231 cells.
