A major focus of systems biology is to characterize interactions between cellular compo- nents, in order to develop an accurate picture of the intricate networks within biological systems. Over the past decade, protei...A major focus of systems biology is to characterize interactions between cellular compo- nents, in order to develop an accurate picture of the intricate networks within biological systems. Over the past decade, protein microarrays have greatly contributed to advances in proteomics and are becoming an important platform for systems biology. Protein microarrays are highly flex- ible, ranging from large-scale proteome microarrays to smaller customizable microarrays, making the technology amenable for detection of a broad spectrum of biochemical properties of proteins. In this article, we will focus on the numerous studies that have utilized protein microarrays to recon- struct biological networks including protein-DNA interactions, posttranslational protein modifica- tions （PTMs）, lectin glycan recognition, pathogen-host interactions and hierarchical signaling cascades. The diversity in applications allows for integration of interaction data from numerous molecular classes and cellular states, providing insight into the structure of complex biological sys- tems. We will also discuss emerging applications and future directions of protein microarray tech- nology in the global frontier.展开更多
Legato公司的NetWorker Business Edition产品包支持8个客户端，支持26个槽位以下的磁带库和多个磁带驱动器．并提供对集群服务器支持等先进的备份功能。NetWorker Business Edition可升级到Network Edition或Power Edition，以支持更...Legato公司的NetWorker Business Edition产品包支持8个客户端，支持26个槽位以下的磁带库和多个磁带驱动器．并提供对集群服务器支持等先进的备份功能。NetWorker Business Edition可升级到Network Edition或Power Edition，以支持更多的备份客户端和SAN的应用，可平滑迁移到Unix或Linux的备份环境而无需对原有备份数据作任何改变。展开更多
This is the first part of an introduction to multicriteria decision making using the Analytic Hierarchy Process (AHP) and its generalization, the Analytic Network Process (ANP). The discussion involves individual and ...This is the first part of an introduction to multicriteria decision making using the Analytic Hierarchy Process (AHP) and its generalization, the Analytic Network Process (ANP). The discussion involves individual and group decisions both with the independence of the criteria from the alternatives as in the AHP and also with dependence and feedback in the entire decision structure as in the ANP. This part explains the Analytic Hierarchy Process, with examples, and presents in some detail the mathematical foundations. An exposition of the Analytic Network Process and its applications will appear in later issues of this journal.展开更多
Chinese medicine(CM) is usually prescribed as CM formula to treat disease. The lack of effective research approach makes it difficult to elucidate the molecular mechanisms of CM formula owing to its complicated chemic...Chinese medicine(CM) is usually prescribed as CM formula to treat disease. The lack of effective research approach makes it difficult to elucidate the molecular mechanisms of CM formula owing to its complicated chemical compounds. Network pharmacology is increasingly applied in CM formula research in recent years, which is identified suitable for the study of CM formula. In this review, we summarized the methodology of network pharmacology, including network construction, network analysis and network verification. The aim of constructing a network is to achieve the interaction between the bioactive compounds and targets and the interaction between various targets, and then find out and validate the key nodes via network analysis and network verification. Besides, we reviewed the application in CM formula research, mainly including targets discovery, bioactive compounds screening, toxicity evaluation, mechanism research and quality control research. Finally, we proposed prospective in the future and limitations of network pharmacology, expecting to provide new strategy and thinking on study for CM formula.展开更多
基金the Grants awarded to HZ (Grant No. RR020839, DK082840,RO1GM076102, CA125807, CA160036 and HG006434)an F31 NRSA Predoctoral Fellowship to IU (Grant No.5F31GM096716)
文摘A major focus of systems biology is to characterize interactions between cellular compo- nents, in order to develop an accurate picture of the intricate networks within biological systems. Over the past decade, protein microarrays have greatly contributed to advances in proteomics and are becoming an important platform for systems biology. Protein microarrays are highly flex- ible, ranging from large-scale proteome microarrays to smaller customizable microarrays, making the technology amenable for detection of a broad spectrum of biochemical properties of proteins. In this article, we will focus on the numerous studies that have utilized protein microarrays to recon- struct biological networks including protein-DNA interactions, posttranslational protein modifica- tions （PTMs）, lectin glycan recognition, pathogen-host interactions and hierarchical signaling cascades. The diversity in applications allows for integration of interaction data from numerous molecular classes and cellular states, providing insight into the structure of complex biological sys- tems. We will also discuss emerging applications and future directions of protein microarray tech- nology in the global frontier.
文摘Legato公司的NetWorker Business Edition产品包支持8个客户端，支持26个槽位以下的磁带库和多个磁带驱动器．并提供对集群服务器支持等先进的备份功能。NetWorker Business Edition可升级到Network Edition或Power Edition，以支持更多的备份客户端和SAN的应用，可平滑迁移到Unix或Linux的备份环境而无需对原有备份数据作任何改变。
文摘This is the first part of an introduction to multicriteria decision making using the Analytic Hierarchy Process (AHP) and its generalization, the Analytic Network Process (ANP). The discussion involves individual and group decisions both with the independence of the criteria from the alternatives as in the AHP and also with dependence and feedback in the entire decision structure as in the ANP. This part explains the Analytic Hierarchy Process, with examples, and presents in some detail the mathematical foundations. An exposition of the Analytic Network Process and its applications will appear in later issues of this journal.
基金Supported by the National Natural Science Foundation of China(No.81530102)Guangdong Provincial Science and Technology Agency Special Funds(No.2017B050504005)Guangzhou City Science and Technology Agency Special Funds(No.201803010069)
文摘Chinese medicine(CM) is usually prescribed as CM formula to treat disease. The lack of effective research approach makes it difficult to elucidate the molecular mechanisms of CM formula owing to its complicated chemical compounds. Network pharmacology is increasingly applied in CM formula research in recent years, which is identified suitable for the study of CM formula. In this review, we summarized the methodology of network pharmacology, including network construction, network analysis and network verification. The aim of constructing a network is to achieve the interaction between the bioactive compounds and targets and the interaction between various targets, and then find out and validate the key nodes via network analysis and network verification. Besides, we reviewed the application in CM formula research, mainly including targets discovery, bioactive compounds screening, toxicity evaluation, mechanism research and quality control research. Finally, we proposed prospective in the future and limitations of network pharmacology, expecting to provide new strategy and thinking on study for CM formula.
