The QTL qTGW3-1 was located on chromosome 3 of rice （Oryza sativa L.） and associated with the 1 000-grain weight （TGW） according to the result of our earlier study. With the objective of fine mapping of this locus...The QTL qTGW3-1 was located on chromosome 3 of rice （Oryza sativa L.） and associated with the 1 000-grain weight （TGW） according to the result of our earlier study. With the objective of fine mapping of this locus, we developed a F2 population consisting of 3 428 plants derived from the cross between TGW-related near isogenic line DL017 （BC3F4 generation of GSL 156×Nipponbare） and the recurrent parent Nipponbare. Using six microsatellites, this QTL was delimited between RM5477 and RM6417. Markers MM 1455 and MM 1456 within this region were used for further mapping of this QTL. Finally, qTGW3-1 was fine-mapped into a 89-kb interval between RM5477 and MM1456, which locates in the BAC clone AC107226 harboring five putative candidate genes.展开更多
Silicon is essential for optimal growth of rice(Oryza sativa L.).This study was conducted to fine map qHUS6.1,a quantitative trait locus(QTL) for rice hull silicon content previously located in the interval RM510-RM19...Silicon is essential for optimal growth of rice(Oryza sativa L.).This study was conducted to fine map qHUS6.1,a quantitative trait locus(QTL) for rice hull silicon content previously located in the interval RM510-RM19417 on the short arm of chromosome 6,and to analyze the effect of this QTL on the silicon content in different organs of rice.Selfed progenies of a residual heterozygous line of rice were detected using 13 microsatellite markers in the vicinity of qHUS6.1.Three plants with overlapping heterozygous segments were selected.Three sets of near isogenic lines(NILs) were developed from the selfed progenies of the 3 plants.They were grown in a paddy field and the silicon contents of the hull,flag leaf,and stem were measured at maturity.Based on analyses of the phenotypic distribution and variance among different genotypic groups in the same NIL set,a significant genotypic effect was shown in the NIL set that was heterogenous in the interval RM19410-RM5815,whereas a significant effect was not found in the remaining 2 NIL sets that were heterogenous in either of the intervals RM4923-RM19410 or RM19417-RM204.On comparison among the physical positions of the 3 heterogenous segments,qHUS6.1 was delimited to a 64.2-kb region flanked by RM19410 and RM19417 that contains nine annotated genes according to the genome sequence of Nipponbare.This QTL showed strong effects on all of the three traits tested,and the enhancing alleles were always derived from the paternal line Milyang 46.The present study will facilitate the cloning of qHUS6.1 and the exploration of new genetic resources for QTL fine mapping.展开更多
基金supported by the National Basic Research Program of China (2010CB129504)the National Key Technologies R&D Program of China (2009BADA2B01)the 948 Project of MOA, China (2011-G2B)
文摘The QTL qTGW3-1 was located on chromosome 3 of rice （Oryza sativa L.） and associated with the 1 000-grain weight （TGW） according to the result of our earlier study. With the objective of fine mapping of this locus, we developed a F2 population consisting of 3 428 plants derived from the cross between TGW-related near isogenic line DL017 （BC3F4 generation of GSL 156×Nipponbare） and the recurrent parent Nipponbare. Using six microsatellites, this QTL was delimited between RM5477 and RM6417. Markers MM 1455 and MM 1456 within this region were used for further mapping of this QTL. Finally, qTGW3-1 was fine-mapped into a 89-kb interval between RM5477 and MM1456, which locates in the BAC clone AC107226 harboring five putative candidate genes.
基金supported by the National High Technology Research and Development Program of China (2009AA101101)the National Natu-ral Science Foundation of China (30571062)
文摘Silicon is essential for optimal growth of rice(Oryza sativa L.).This study was conducted to fine map qHUS6.1,a quantitative trait locus(QTL) for rice hull silicon content previously located in the interval RM510-RM19417 on the short arm of chromosome 6,and to analyze the effect of this QTL on the silicon content in different organs of rice.Selfed progenies of a residual heterozygous line of rice were detected using 13 microsatellite markers in the vicinity of qHUS6.1.Three plants with overlapping heterozygous segments were selected.Three sets of near isogenic lines(NILs) were developed from the selfed progenies of the 3 plants.They were grown in a paddy field and the silicon contents of the hull,flag leaf,and stem were measured at maturity.Based on analyses of the phenotypic distribution and variance among different genotypic groups in the same NIL set,a significant genotypic effect was shown in the NIL set that was heterogenous in the interval RM19410-RM5815,whereas a significant effect was not found in the remaining 2 NIL sets that were heterogenous in either of the intervals RM4923-RM19410 or RM19417-RM204.On comparison among the physical positions of the 3 heterogenous segments,qHUS6.1 was delimited to a 64.2-kb region flanked by RM19410 and RM19417 that contains nine annotated genes according to the genome sequence of Nipponbare.This QTL showed strong effects on all of the three traits tested,and the enhancing alleles were always derived from the paternal line Milyang 46.The present study will facilitate the cloning of qHUS6.1 and the exploration of new genetic resources for QTL fine mapping.
