Chitosan is a nontoxic natural polysaccharide with good biocompatibility.It can form polymer\|metal complexes with many metal cations for its extra electron pair of nitrogen.The complexes exhibit high sorptive capacit...Chitosan is a nontoxic natural polysaccharide with good biocompatibility.It can form polymer\|metal complexes with many metal cations for its extra electron pair of nitrogen.The complexes exhibit high sorptive capacity and good selectivity for urea.The sorption capacity was influenced by the metal content in the complex,time of sorption,concentration of urea etc.The sorption capacities were 83.6,79.1,72.9,69.4 and 53.4 mg/g for urea by the chitosan metal complexes of Cu(Ⅱ),Cd(Ⅱ),Co(Ⅱ),Zn(Ⅱ) and Fe(Ⅲ),respectively at pH 6.0 and the concentration of urea being 1 300 mg/L.展开更多
目的：从蕨菜中分离纯化多糖成分，并进行相对分子质量、单糖组成和免疫调节活性研究。方法：采用热水浸提法提取蕨菜粗多糖，再经脱蛋白、DEAE Sepharose Fast Flow和Sepharose 4B凝胶柱层析得到3种均一多糖组分PLP1、PLP2和PLP3；采...目的：从蕨菜中分离纯化多糖成分，并进行相对分子质量、单糖组成和免疫调节活性研究。方法：采用热水浸提法提取蕨菜粗多糖，再经脱蛋白、DEAE Sepharose Fast Flow和Sepharose 4B凝胶柱层析得到3种均一多糖组分PLP1、PLP2和PLP3；采用高效液相凝胶渗透色谱法测定均一多糖相对分子质量；采用气相色谱分析均一多糖的单糖组分，采用MTT法和Griess法检测多糖对RAW264.7细胞生长和释放NO能力的影响。结果：PLP1、PLP2和PLP3的相对分子质量分别为6.62×10^4、4.34×10^5、1.88×10^5；PLP1含有鼠李糖、阿拉伯糖、岩藻糖、木糖、甘露糖、葡萄糖和半乳糖，PLP2含有鼠李糖、阿拉伯糖、岩藻糖、木糖、甘露糖和半乳糖，PLP3含有鼠李糖、阿拉伯糖、岩藻糖、木糖、甘露糖和半乳糖。3种多糖均可显著促进RAW264.7细胞的增殖，均具有显著增强RAW264.7细胞释放NO的能力，并呈一定的剂量效应关系。结论：PLP1、PLP2和PLP3 3个组分均为新的天然来源的均一杂多糖。3种多糖体外均能激活巨噬细胞，呈现显著的免疫调节活性。展开更多
文摘Chitosan is a nontoxic natural polysaccharide with good biocompatibility.It can form polymer\|metal complexes with many metal cations for its extra electron pair of nitrogen.The complexes exhibit high sorptive capacity and good selectivity for urea.The sorption capacity was influenced by the metal content in the complex,time of sorption,concentration of urea etc.The sorption capacities were 83.6,79.1,72.9,69.4 and 53.4 mg/g for urea by the chitosan metal complexes of Cu(Ⅱ),Cd(Ⅱ),Co(Ⅱ),Zn(Ⅱ) and Fe(Ⅲ),respectively at pH 6.0 and the concentration of urea being 1 300 mg/L.
