A novel approach of combining conventional infrared spectroscopy （IR） and atomic force microscopy （AFM） is presented to better understand the behavior of a drug adsorbed on a metal substrate at the nanoscale level...A novel approach of combining conventional infrared spectroscopy （IR） and atomic force microscopy （AFM） is presented to better understand the behavior of a drug adsorbed on a metal substrate at the nanoscale level. Tip-enhanced infrared nanospectroscopy （TEIRA） was used for the first time to investigate Lu AA33810, a selective brain-penetrating Y5 receptor antagonist, after immobilization on gold nanopartides （GNPs）. Here, a gold coated AFM tip and gold substrate were used to obtain the near-field electromagnetic field trapping effect. Because of the huge signal enhancement, it was possible to obtain the spectral information regarding the self-assembled monolayer of the investigated molecule. The effect of two orthogonal polarizations （p- and s-polarization modulations） of the excitation laser beam on the spectral patterns is also discussed. The results show that there is a strong relationship between the state of polarization of the incident radiation and the relative infrared band intensities. Another factor affecting the observed spectral differences is the topology of the metal substrate, which may result in the induction of a cross-polarization effect. The performed analysis indicates that the C--C bond from the cyclohexyl group is oriented almost parallel to the metal surface. Conversely, the p- and s-polarized spectral variations suggest that the O=S---O angle is high enough to enable the simultaneous interaction of both oxygen atoms with the GNPs.展开更多
We review the recent biomedical detection developments of scanning near-field optical microscopy(SNOM),focusing on scattering-type SNOM,atomic force microscope-based infrared spectroscopy,peak force infrared microscop...We review the recent biomedical detection developments of scanning near-field optical microscopy(SNOM),focusing on scattering-type SNOM,atomic force microscope-based infrared spectroscopy,peak force infrared microscopy,and photo-induced force microscopy,which have the advantages of label-free,noninvasive,and specific spectral recognition.Considering the high water content of biological samples and the strong absorption of water by infrared waves,we divide the relevant research on these techniques into two categories:one based on a nonliquid environment and the other based on a liquid environment.In the nonliquid environment,the chemical composition and structural information of biomedical samples can be obtained with nanometer resolution.In the liquid environment,these techniques can be used to monitor the dynamic chemical reaction process and track the process of chemical composition and structural change of single molecules,which is conducive to exploring the development mechanism of physiological processes.We elaborate their experimental challenges,technical means,and actual cases for three microbiomedical samples(including biomacromolecules,cells,and tissues).We also discuss the prospects and challenges for their development.Our work lays a foundation for the rational design and efficient use of near-field optical microscopy to explore the characteristics of microscopic biology.展开更多
Tip-enhanced infrared(IR)spectra and imaging have been widely used in cutting-edge studies for the in-depth understanding of the composition,structure,and function of interfaces at the nanoscale.However,molecular mono...Tip-enhanced infrared(IR)spectra and imaging have been widely used in cutting-edge studies for the in-depth understanding of the composition,structure,and function of interfaces at the nanoscale.However,molecular monolayer sensitivity has only been demonstrated at solid/gas interfaces.In an aqueous environment,reduced sensitivity limits the practical applications of tip-enhanced IR nanospectroscopy.Here,we present an approach to hypersensitive nanoscale IR spectra and imaging in an aqueous environment with a combination of photoinduced force(PiF)microscopy and resonant antennas.展开更多
The application of metal nanoparticles as an efficient drug delivery system is one of the directions of cancer therapy development.However,this strategy requires precise information about how the drug interacts with t...The application of metal nanoparticles as an efficient drug delivery system is one of the directions of cancer therapy development.However,this strategy requires precise information about how the drug interacts with the applied nanocarrier.In this study,atomic force microscopy combined with infrared spectroscopy(AFM-IR)was used for the first time to investigate the erlotinib adsorption structure on two different types of 15 nm metal nanoparticle mono-layers,namely,silver nanoparticle(AgNP)and gold nanoparticle(AuNP)mono-layers.Because the metal nanoparticles are loosely bound samples,only the tapping AFM-IR mode is suitable for the collection of IR maps and spectra for such a system.The obtained results indicated the relevance of the AFM-IR technique for characterizing drug interactions with a metal mono-layer surface.The investigated drug interacts with the AgNPs mainly through phenyl rings and methoxy moieties,while quinazoline,amino,and ethoxy moieties appear to be farther from the surface.For the AuNPs,the interaction occurs through both the phenyl ring and the quinazoline moiety.Additionally,the aliphatic groups of erlotinib directly participate in this interaction.The novelty of the present work is also related to the use of the tapping AFM-IR mode to study metal NP mono-layers with a drug adsorbed on them.The collected IR maps for the most enhanced erlotinib bands show specific areas with very high signal intensity.The connection between these areas and the"hot spots"typical for the surface plasmon resonance phenomenon of metals is considered.展开更多
The performance of functional materials and specifically energy-related functional materials,such as fuel cells,solar cells,batteries and catalysts depends on the their local,nanoscale properties.However,heterogeneiti...The performance of functional materials and specifically energy-related functional materials,such as fuel cells,solar cells,batteries and catalysts depends on the their local,nanoscale properties.However,heterogeneities in the phase,structure and composition of these functional materials makes it difficult to directly monitor and identify the influence of local physicochemical parameters on their global functionality.In this review we will discuss recent developments in the field of IR nanospectroscopy that enables the extraction of detailed chemical information at the nanoscale and the identification of nanoscale properties that influence the global performances of functional materials.Specifically,we will discuss the ways by which infrared (IR) nanospectroscopy techniques,namely photo thermal induced resonance (PTIR) and scattering scanning near-field optical microscopy (s-SNOM),are utilized in order to identify nanoscale properties and their influence on the functionality of halide-perovskite solar cells and catalytic nanoparticles.In the last part of this review we will address the technical challenges and opportunities in expanding the scope of IR nanospectroscopy measurements into the field of electrochemistry-based functional materials.展开更多
文摘A novel approach of combining conventional infrared spectroscopy （IR） and atomic force microscopy （AFM） is presented to better understand the behavior of a drug adsorbed on a metal substrate at the nanoscale level. Tip-enhanced infrared nanospectroscopy （TEIRA） was used for the first time to investigate Lu AA33810, a selective brain-penetrating Y5 receptor antagonist, after immobilization on gold nanopartides （GNPs）. Here, a gold coated AFM tip and gold substrate were used to obtain the near-field electromagnetic field trapping effect. Because of the huge signal enhancement, it was possible to obtain the spectral information regarding the self-assembled monolayer of the investigated molecule. The effect of two orthogonal polarizations （p- and s-polarization modulations） of the excitation laser beam on the spectral patterns is also discussed. The results show that there is a strong relationship between the state of polarization of the incident radiation and the relative infrared band intensities. Another factor affecting the observed spectral differences is the topology of the metal substrate, which may result in the induction of a cross-polarization effect. The performed analysis indicates that the C--C bond from the cyclohexyl group is oriented almost parallel to the metal surface. Conversely, the p- and s-polarized spectral variations suggest that the O=S---O angle is high enough to enable the simultaneous interaction of both oxygen atoms with the GNPs.
基金supported by the National Key Research and Development Program(Grant No.2022YFA1404004)the Key Domestic Scientific and Technological Cooperation Projects in Shanghai(Grant No.21015800200).
文摘We review the recent biomedical detection developments of scanning near-field optical microscopy(SNOM),focusing on scattering-type SNOM,atomic force microscope-based infrared spectroscopy,peak force infrared microscopy,and photo-induced force microscopy,which have the advantages of label-free,noninvasive,and specific spectral recognition.Considering the high water content of biological samples and the strong absorption of water by infrared waves,we divide the relevant research on these techniques into two categories:one based on a nonliquid environment and the other based on a liquid environment.In the nonliquid environment,the chemical composition and structural information of biomedical samples can be obtained with nanometer resolution.In the liquid environment,these techniques can be used to monitor the dynamic chemical reaction process and track the process of chemical composition and structural change of single molecules,which is conducive to exploring the development mechanism of physiological processes.We elaborate their experimental challenges,technical means,and actual cases for three microbiomedical samples(including biomacromolecules,cells,and tissues).We also discuss the prospects and challenges for their development.Our work lays a foundation for the rational design and efficient use of near-field optical microscopy to explore the characteristics of microscopic biology.
基金supported by grants from the National Key Research and Development Program of China(no.2017YFA0206500)the National Natural Science Foundation of China(no.21635004,22004069)+1 种基金the Excellent Research Program of Nanjing University(no.ZYJH004)the State Key Laboratory of Analytical Chemistry for Life Science(no.5431ZZXM2001).
文摘Tip-enhanced infrared(IR)spectra and imaging have been widely used in cutting-edge studies for the in-depth understanding of the composition,structure,and function of interfaces at the nanoscale.However,molecular monolayer sensitivity has only been demonstrated at solid/gas interfaces.In an aqueous environment,reduced sensitivity limits the practical applications of tip-enhanced IR nanospectroscopy.Here,we present an approach to hypersensitive nanoscale IR spectra and imaging in an aqueous environment with a combination of photoinduced force(PiF)microscopy and resonant antennas.
基金This work was supported by the National Science Centre Poland(No.2016/21/D/ST4/02178 to N.P.).N.P.gratefully acknowledges the financial support of the French Government and the French Embassy in Poland.These researches were also supported by the Paris Ile-de-France Region-DIM Materiaux anciens et patrim oniaux.The m easurem ents were partly perform ed using the equipm ent purchased in the frame of the project co-funded by the MatopolskaRegional Operational Program Measure 5.1 Krakow Metropolitan Areaas an important hub of the European Research Area for 2007-2013,project no.MRP0.05.01.00-12-013/15e.
文摘The application of metal nanoparticles as an efficient drug delivery system is one of the directions of cancer therapy development.However,this strategy requires precise information about how the drug interacts with the applied nanocarrier.In this study,atomic force microscopy combined with infrared spectroscopy(AFM-IR)was used for the first time to investigate the erlotinib adsorption structure on two different types of 15 nm metal nanoparticle mono-layers,namely,silver nanoparticle(AgNP)and gold nanoparticle(AuNP)mono-layers.Because the metal nanoparticles are loosely bound samples,only the tapping AFM-IR mode is suitable for the collection of IR maps and spectra for such a system.The obtained results indicated the relevance of the AFM-IR technique for characterizing drug interactions with a metal mono-layer surface.The investigated drug interacts with the AgNPs mainly through phenyl rings and methoxy moieties,while quinazoline,amino,and ethoxy moieties appear to be farther from the surface.For the AuNPs,the interaction occurs through both the phenyl ring and the quinazoline moiety.Additionally,the aliphatic groups of erlotinib directly participate in this interaction.The novelty of the present work is also related to the use of the tapping AFM-IR mode to study metal NP mono-layers with a drug adsorbed on them.The collected IR maps for the most enhanced erlotinib bands show specific areas with very high signal intensity.The connection between these areas and the"hot spots"typical for the surface plasmon resonance phenomenon of metals is considered.
文摘The performance of functional materials and specifically energy-related functional materials,such as fuel cells,solar cells,batteries and catalysts depends on the their local,nanoscale properties.However,heterogeneities in the phase,structure and composition of these functional materials makes it difficult to directly monitor and identify the influence of local physicochemical parameters on their global functionality.In this review we will discuss recent developments in the field of IR nanospectroscopy that enables the extraction of detailed chemical information at the nanoscale and the identification of nanoscale properties that influence the global performances of functional materials.Specifically,we will discuss the ways by which infrared (IR) nanospectroscopy techniques,namely photo thermal induced resonance (PTIR) and scattering scanning near-field optical microscopy (s-SNOM),are utilized in order to identify nanoscale properties and their influence on the functionality of halide-perovskite solar cells and catalytic nanoparticles.In the last part of this review we will address the technical challenges and opportunities in expanding the scope of IR nanospectroscopy measurements into the field of electrochemistry-based functional materials.
