Intervaginal space injection(ISI)is a novel mode of administration investigated over the last decade.After injecting nanoparticles into the intervaginal space,they can be transported along low flow resistance channels...Intervaginal space injection(ISI)is a novel mode of administration investigated over the last decade.After injecting nanoparticles into the intervaginal space,they can be transported along low flow resistance channels into the interstitial space.This transport has a certain delivery direction,and site-specific injection can work on specific organs or tissues.In this study,the thorax,a new ISI site in the interstitial surrounding the internal thoracic artery named the thoracic interstitial injection(tISI)was investigated.To prove the targeting ability of the tISI,two sizes of gold nanoparticles(AuNPs)(47 and 87 nm)were administered to mice.After 1 h,the biodistribution of AuNPs in the tissues was measured via single particle inductively coupled plasma mass spectrometry(spICP-MS).The results showed that the concentration of AuNPs in the aorta after tISI injection was significantly higher than that after intravenous injection.Moreover,fewer nanoparticles with larger particle sizes were observed to have entered the blood and were better targeted to the aorta.Thereafter,tanshinone IIa sodium sulfonate liposomes were administered for the treatment of aortic atherosclerosis.The proportion of aortic plaques in atherosclerotic Apoe-/-mice administered via tISI was significantly lower than that in other model animals(P<0.001).Furthermore,the proteoglycan content and CD68-positive cell count in the plaques were significantly reduced.The vascular elastic fibers at the plaque site were thickened,and fractures were reduced.tISI was,therefore,determined to be an effective strategy for the treatment of atherosclerotic aortic plaques.展开更多
In the present study we carried out the synthesis of β-cyclodextrin （β-CD） functionalized gold nanoparticles （AuNPs） using a microwave assisted heating method in alkaline media. Stable dispersion of β-CD stabil...In the present study we carried out the synthesis of β-cyclodextrin （β-CD） functionalized gold nanoparticles （AuNPs） using a microwave assisted heating method in alkaline media. Stable dispersion of β-CD stabilized AuNPs was obtained at an optimized pH of 10,5. At this pH value the deprotonated secondary hydroxyl group of β-CD shows the highest chelating affinity toward Pb2＋ ions thereby inducing AuNP aggregation. The Pb2＋ induced aggregation in β-CD-AuNP solution is monitored by both colorimetric response and UV-Vis spectroscopy. TEM, DLS and FTIR analyses were carried out to confirm the Pb2＋ ion induced aggregation behaviour of β-CD-AuNPs under alkaline conditions. Furthermore at the experimental pH the response of the β-CD-AuNP system towards Pb2＋ ions is selective when compared with other interfering metal cations.展开更多
基金supported by the Key Research Program of Frontier Science of CAS(No.ZDBS-LY-SLH036)Key deployment projects of CAS(No.QYKJZD-SSW-SLH02).
文摘Intervaginal space injection(ISI)is a novel mode of administration investigated over the last decade.After injecting nanoparticles into the intervaginal space,they can be transported along low flow resistance channels into the interstitial space.This transport has a certain delivery direction,and site-specific injection can work on specific organs or tissues.In this study,the thorax,a new ISI site in the interstitial surrounding the internal thoracic artery named the thoracic interstitial injection(tISI)was investigated.To prove the targeting ability of the tISI,two sizes of gold nanoparticles(AuNPs)(47 and 87 nm)were administered to mice.After 1 h,the biodistribution of AuNPs in the tissues was measured via single particle inductively coupled plasma mass spectrometry(spICP-MS).The results showed that the concentration of AuNPs in the aorta after tISI injection was significantly higher than that after intravenous injection.Moreover,fewer nanoparticles with larger particle sizes were observed to have entered the blood and were better targeted to the aorta.Thereafter,tanshinone IIa sodium sulfonate liposomes were administered for the treatment of aortic atherosclerosis.The proportion of aortic plaques in atherosclerotic Apoe-/-mice administered via tISI was significantly lower than that in other model animals(P<0.001).Furthermore,the proteoglycan content and CD68-positive cell count in the plaques were significantly reduced.The vascular elastic fibers at the plaque site were thickened,and fractures were reduced.tISI was,therefore,determined to be an effective strategy for the treatment of atherosclerotic aortic plaques.
文摘In the present study we carried out the synthesis of β-cyclodextrin （β-CD） functionalized gold nanoparticles （AuNPs） using a microwave assisted heating method in alkaline media. Stable dispersion of β-CD stabilized AuNPs was obtained at an optimized pH of 10,5. At this pH value the deprotonated secondary hydroxyl group of β-CD shows the highest chelating affinity toward Pb2＋ ions thereby inducing AuNP aggregation. The Pb2＋ induced aggregation in β-CD-AuNP solution is monitored by both colorimetric response and UV-Vis spectroscopy. TEM, DLS and FTIR analyses were carried out to confirm the Pb2＋ ion induced aggregation behaviour of β-CD-AuNPs under alkaline conditions. Furthermore at the experimental pH the response of the β-CD-AuNP system towards Pb2＋ ions is selective when compared with other interfering metal cations.
