A neutral metalloprotease was purified from the cultured mycelia of Laccocephalum mylittae,an effective medicinal fungus widely used in anthelmintic therapy.The protease was purified to homogeneity with 31.85-fold pur...A neutral metalloprotease was purified from the cultured mycelia of Laccocephalum mylittae,an effective medicinal fungus widely used in anthelmintic therapy.The protease was purified to homogeneity with 31.85-fold purification and a final yield of 21.76%.The subunit molecular weight of the protease is about 40000 estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE).The optimum reaction pH and temperature are 7.5 and 50oC,respectively.The protease activity is largely enhanced by Ca2 +,but highly inhibited by tetrasodium ethylenediaminetetraacetate(EDTA),a metal-chelator,suggesting that the enzyme is a metalloprotease.The Michaelis-Menten constan Km and Vmax value for casein substrate are 6.09 mg·ml -1and 21.32μg·min -1·ml -1, respectively.In vitro anthelmintic tests of the protease exhibit distinct lethal effects on the third stage larvae(L3)of Ascaris suum.Scanning electron microscopy and SDS-PAGE analysis indicates that the proteolysis of larvae proteins caused by this protease may relate to the anthelmintic activity of L.mylittae.展开更多
Commercially important polyphagous tropical tasar silk insect, Antheraea mylitta Drury is distributed along central India （12-31 ° N latitude, 72-96° E longitude）, which on adaptation formed in to forty fo...Commercially important polyphagous tropical tasar silk insect, Antheraea mylitta Drury is distributed along central India （12-31 ° N latitude, 72-96° E longitude）, which on adaptation formed in to forty four ecoraces of varied phenotypes. Its wild ecoraces like Raily, Modal, Jata, Sarihan, Laria, Bhandara and Andhra, besides domesticated Daba and Sukinda are contributing for livelihood of forest based aboriginals. However, the deforestation and extensive collection of nature grown cocoons led to declination of wild population and even have endangered few ecoraces. The government and Non Government Organizations （NGOs）, although promote ecorace conservation, it could upkeep the insect wildlife only to limited extent. But the forest department with associated self help societies has advantage in conserving tasar insect as prospective forest wealth, with better knowledge and access to its habitats. The co-ordinate efforts from forest, sericulture departments to participate native aboriginals of such societies can facilitate conserving tasar-biodiversity as most feasible forest associated resource.展开更多
基金Supported by the National High Technology Research and Development Program of China（2007AA021506） the Natural Science Foundation of Zhejiang Province（R207609）
文摘A neutral metalloprotease was purified from the cultured mycelia of Laccocephalum mylittae,an effective medicinal fungus widely used in anthelmintic therapy.The protease was purified to homogeneity with 31.85-fold purification and a final yield of 21.76%.The subunit molecular weight of the protease is about 40000 estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE).The optimum reaction pH and temperature are 7.5 and 50oC,respectively.The protease activity is largely enhanced by Ca2 +,but highly inhibited by tetrasodium ethylenediaminetetraacetate(EDTA),a metal-chelator,suggesting that the enzyme is a metalloprotease.The Michaelis-Menten constan Km and Vmax value for casein substrate are 6.09 mg·ml -1and 21.32μg·min -1·ml -1, respectively.In vitro anthelmintic tests of the protease exhibit distinct lethal effects on the third stage larvae(L3)of Ascaris suum.Scanning electron microscopy and SDS-PAGE analysis indicates that the proteolysis of larvae proteins caused by this protease may relate to the anthelmintic activity of L.mylittae.
文摘Commercially important polyphagous tropical tasar silk insect, Antheraea mylitta Drury is distributed along central India （12-31 ° N latitude, 72-96° E longitude）, which on adaptation formed in to forty four ecoraces of varied phenotypes. Its wild ecoraces like Raily, Modal, Jata, Sarihan, Laria, Bhandara and Andhra, besides domesticated Daba and Sukinda are contributing for livelihood of forest based aboriginals. However, the deforestation and extensive collection of nature grown cocoons led to declination of wild population and even have endangered few ecoraces. The government and Non Government Organizations （NGOs）, although promote ecorace conservation, it could upkeep the insect wildlife only to limited extent. But the forest department with associated self help societies has advantage in conserving tasar insect as prospective forest wealth, with better knowledge and access to its habitats. The co-ordinate efforts from forest, sericulture departments to participate native aboriginals of such societies can facilitate conserving tasar-biodiversity as most feasible forest associated resource.
