To investigate the immune responses to the attenuated Mycoplasma hyopneumoniae 168 strain vaccine, 8-15 d old piglets were immunized with M. hyopneurnoniae 168 strain vaccine by intrapulmonic route. And the specific I...To investigate the immune responses to the attenuated Mycoplasma hyopneumoniae 168 strain vaccine, 8-15 d old piglets were immunized with M. hyopneurnoniae 168 strain vaccine by intrapulmonic route. And the specific IgG antibody in serum, lymphoproliferation, IFNT, and specific secretory IgA （SIgA） antibody in bronchoalveolar lavage fluid were detected on 30 and 60 d post-immunization （DPI）, respectively. On 60 DPI, all the pigs except for those in health control group were challenged with a field M. hyopneumoniae strain JS. Necropsy was performed on 30 d post-challenge （DPC）. The results showed that IFN7 and specific SIgA were stimulated on surface of respiratory tract after immunization. And peripheral blood mononuclear cells could also be proliferated about 1.81 and 2.12 fold on 30 and 60 DPI when stimulated by M. hyopneumoniae protein in vitro. However, no serum IgG antibody against M. hyopneumoniae was detected during the whole immune phage. After challenge, vaccinated pigs were observed with only very slight histological lesion in individual lobes. None of vaccinated pigs showed any clinical signs. While the unvaccinated pigs from challenge control group showed varying degrees of clinical sign and severe macroscopical lesion of mycoplasmal pneumonia of swine （MPS）. The result suggested that the attenuated M. hyopneumoniae 168 strain vaccine inoculated by intrapulmonic route could activate the systemic cellular immunity, the local mucosal immunity and IFNγ secretion in respiratory tract to against M. hyopneumoniae infection in piglets.展开更多
猪肺炎支原体(Mycoplasma hyopneumoniae,Mhp)是引起猪支原体肺炎(Mycoplasma pneumoniae of Swine,MPS)的病原,给养猪业造成巨大的经济损失。由于对具有良好免疫原性的Mhp特异性蛋白缺少深入研究,使得Mhp在致病机制、血清学检测方法和...猪肺炎支原体(Mycoplasma hyopneumoniae,Mhp)是引起猪支原体肺炎(Mycoplasma pneumoniae of Swine,MPS)的病原,给养猪业造成巨大的经济损失。由于对具有良好免疫原性的Mhp特异性蛋白缺少深入研究,使得Mhp在致病机制、血清学检测方法和新型疫苗的研究发展上受到一定限制,从而影响了对MPS的控制。本研究综合Mhp主要粘附因子、膜蛋白及细胞质蛋白等主要抗原蛋白的研究进展,为该病原致病机理研究、建立特异性诊断方法和推动基因工程疫苗发展提供一定的理论基础,对MPS的正确诊断、检测、免疫预防和控制也有重要意义。展开更多
基金supported by the Natural Science Foundation of Jiangsu Province, China (BK2007711)the China Postdoctoral Fundation (20070421022)the Three Agricultural Projects Fundation of Jiangsu Province, China (SX2007082)
文摘To investigate the immune responses to the attenuated Mycoplasma hyopneumoniae 168 strain vaccine, 8-15 d old piglets were immunized with M. hyopneurnoniae 168 strain vaccine by intrapulmonic route. And the specific IgG antibody in serum, lymphoproliferation, IFNT, and specific secretory IgA （SIgA） antibody in bronchoalveolar lavage fluid were detected on 30 and 60 d post-immunization （DPI）, respectively. On 60 DPI, all the pigs except for those in health control group were challenged with a field M. hyopneumoniae strain JS. Necropsy was performed on 30 d post-challenge （DPC）. The results showed that IFN7 and specific SIgA were stimulated on surface of respiratory tract after immunization. And peripheral blood mononuclear cells could also be proliferated about 1.81 and 2.12 fold on 30 and 60 DPI when stimulated by M. hyopneumoniae protein in vitro. However, no serum IgG antibody against M. hyopneumoniae was detected during the whole immune phage. After challenge, vaccinated pigs were observed with only very slight histological lesion in individual lobes. None of vaccinated pigs showed any clinical signs. While the unvaccinated pigs from challenge control group showed varying degrees of clinical sign and severe macroscopical lesion of mycoplasmal pneumonia of swine （MPS）. The result suggested that the attenuated M. hyopneumoniae 168 strain vaccine inoculated by intrapulmonic route could activate the systemic cellular immunity, the local mucosal immunity and IFNγ secretion in respiratory tract to against M. hyopneumoniae infection in piglets.
文摘猪肺炎支原体(Mycoplasma hyopneumoniae,Mhp)是引起猪支原体肺炎(Mycoplasma pneumoniae of Swine,MPS)的病原,给养猪业造成巨大的经济损失。由于对具有良好免疫原性的Mhp特异性蛋白缺少深入研究,使得Mhp在致病机制、血清学检测方法和新型疫苗的研究发展上受到一定限制,从而影响了对MPS的控制。本研究综合Mhp主要粘附因子、膜蛋白及细胞质蛋白等主要抗原蛋白的研究进展,为该病原致病机理研究、建立特异性诊断方法和推动基因工程疫苗发展提供一定的理论基础,对MPS的正确诊断、检测、免疫预防和控制也有重要意义。
文摘根据猪肺炎支原体(Mhp)和猪鼻支原体(Mhr)的16S rRNA基因设计3条引物,建立Mhp和Mhr的双重PCR检测方法,并对该方法进行了特异性和敏感性试验。应用建立的方法检测了临床样品和疫苗样品。结果显示,该方法具有良好的特异性,最低可检测到0.66 ng的Mhp基因组DNA和0.58 ng Mhr基因组DNA。临床样品和疫苗样品检测结果与普通PCR检测结果一致。该双重PCR方法可用于Mhp与Mhr的鉴别、诊断以及疫苗纯粹性检查,快速而准确。
