期刊文献+
共找到655篇文章
< 1 2 33 >
每页显示 20 50 100
西藏山南地区牦牛多杀性巴氏杆菌的分离鉴定及药敏试验 被引量:7
1
作者 崔爱莲 吴庆侠 +1 位作者 董海龙 朱洪云 《畜牧与兽医》 北大核心 2016年第7期146-147,共2页
牛巴氏杆菌病又称牛出血性败血症(HSC),简称"牛出败",是由多杀性巴氏杆菌(Pasteurella multocida,Pm)引起的一种急性传染病,以高热肺炎、急性胃肠炎以及内脏器官广泛出血为特征牛传染病。作为全国五大牧区之一的西藏,牦牛作为牧... 牛巴氏杆菌病又称牛出血性败血症(HSC),简称"牛出败",是由多杀性巴氏杆菌(Pasteurella multocida,Pm)引起的一种急性传染病,以高热肺炎、急性胃肠炎以及内脏器官广泛出血为特征牛传染病。作为全国五大牧区之一的西藏,牦牛作为牧区的主导产业,是以我国青藏高原为中心产区,主要分布在喜马拉雅山脉和青藏高原的特有地方家畜品种。 展开更多
关键词 多杀性巴氏杆菌 牛巴氏杆菌病 药敏试验 出血性败血症 急性传染 multocida PASTEURELLA 主导产业 鲜血琼脂 革兰染色镜检
原文传递
多杀性巴氏杆菌诊断技术及防控策略研究进展 被引量:1
2
作者 李昕 张正刚 +2 位作者 郭亚男 宗亮泽 梁小军 《中南农业科技》 2023年第10期218-221,共4页
多杀性巴氏杆菌是动物呼吸道常见的致病细菌,在世界范围内严重威胁牲畜健康。该病原广泛存在于各种健康动物的鼻腔、咽及肺等部位,当机体受到外界不良刺激造成免疫水平下降时,该菌大量繁殖并引发疾病。从多杀性巴氏杆菌培养特性、发病... 多杀性巴氏杆菌是动物呼吸道常见的致病细菌,在世界范围内严重威胁牲畜健康。该病原广泛存在于各种健康动物的鼻腔、咽及肺等部位,当机体受到外界不良刺激造成免疫水平下降时,该菌大量繁殖并引发疾病。从多杀性巴氏杆菌培养特性、发病机制、诊断技术和防控策略等方面进行综述,为多杀性巴氏杆菌疾病的防治奠定基础。 展开更多
关键词 多杀性巴氏杆菌(Pasteurella multocida) 培养特性 诊断技术 防控策略
下载PDF
猪A型多杀性巴氏杆菌的分离鉴定及耐药性分析 被引量:5
3
作者 张显浩 陈敏鸿 +5 位作者 李冰 蔡佳跃 黎云裕 裴仉福 陈瑞爱 贺东生 《黑龙江畜牧兽医(下半月)》 北大核心 2016年第1期110-112,共3页
多杀性巴氏杆菌(Pasteurella multocida,Pm)能够引起畜禽等多种动物及人类感染发病[1]。本菌可在猪群中广泛传播和存在,主要引起猪肺疫和进行性萎缩性鼻炎(Progressive atrophic rhinitis,PAR)[2],且可与多种病原混合协同感染,从而... 多杀性巴氏杆菌(Pasteurella multocida,Pm)能够引起畜禽等多种动物及人类感染发病[1]。本菌可在猪群中广泛传播和存在,主要引起猪肺疫和进行性萎缩性鼻炎(Progressive atrophic rhinitis,PAR)[2],且可与多种病原混合协同感染,从而增加猪群呼吸道疾病的发病率和死亡率,造成严重的经济损失。 展开更多
关键词 多杀性巴氏杆菌 萎缩性鼻炎 猪肺疫 RHINITIS multocida PASTEURELLA 类感染 抗原成分 ATROPHIC 生化鉴定管
原文传递
一例山羊链球菌与多杀性巴氏杆菌混合感染的调查分析 被引量:2
4
作者 潘艳 贺会利 +2 位作者 冯世文 李军 袁翠平 《湖北农业科学》 2021年第23期123-126,共4页
对2020年8月广西某养羊场送检的1份羊病料采用细菌学和分子生物学方法进行诊断,对分离菌株进行形态学特性、生理生化、16S rDNA测序,结果鉴定为链球菌,应用PCR方法检测到多杀性巴氏杆菌阳性。对分离菌进行药敏试验,结果显示该菌对青霉... 对2020年8月广西某养羊场送检的1份羊病料采用细菌学和分子生物学方法进行诊断,对分离菌株进行形态学特性、生理生化、16S rDNA测序,结果鉴定为链球菌,应用PCR方法检测到多杀性巴氏杆菌阳性。对分离菌进行药敏试验,结果显示该菌对青霉素、恩诺沙星、氧氟沙星、土霉素、头孢噻呋钠高度敏感。对分离菌进行致病性试验,结果显示该菌有较强的致病性。检测结果表明该病为链球菌与多杀性巴氏杆菌混合感染引起的。 展开更多
关键词 细菌学 链球菌(Streptococcus) 多杀性巴氏杆菌(Pasteurella multocida) 药敏试验 致病性
下载PDF
Development of Multiplex-PCR for Identification of Pasteurella multocida,Haemophilus parasuis and Actinbacillus pleuropneumoniae 被引量:2
5
作者 HE Ying,ZHAO Ping,CHU Yue-feng,GAO Peng-cheng,LU Zhong-xin Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences State Key Laboratory of Veterinary Etiological Biology +1 位作者 Key laboratory of Grazing Animal Diseases of Ministry of Agriculture Key Laboratory of Animal Virology of Ministry of Agriculture,Lanzhou 730046,China 《Animal Husbandry and Feed Science》 CAS 2010年第8期25-27,共3页
[ Objective] To develop multiplex-PCR for diagnosis of mixed infection caused by Pasteurella multocida ( PM), Haomophilus parasuis (HPS) and Actinbaci/lus pleuropneumoniae (App). [ Method ] PCR method was develo... [ Objective] To develop multiplex-PCR for diagnosis of mixed infection caused by Pasteurella multocida ( PM), Haomophilus parasuis (HPS) and Actinbaci/lus pleuropneumoniae (App). [ Method ] PCR method was developed to detect single infection caused by PM, HPS or App. The conditions of amplification and primers were optimized, and the multiple-PCR was developed to detect mixed infection of PM, HPS and App. [ Result] A 457-bp band, a 821-bp band and a 342-bp band were simultaneously amplified in the one PCR reaction system. The method had high sensitivity and specificity. [ Conduslon] The multiple-PCR is successfully developed and can be used for differential diagnosis of PM, HPS and App. 展开更多
关键词 Haemophilus parasuis Pasteurella multocida Actinbacillus pleuropneumoniae MULTIPLEX-PCR
下载PDF
Construction and Virulence of Filamentous Hemagglutinin Protein B1 Mutant of Pasteurella multocida in Chickens 被引量:2
6
作者 GUO Dong-chun SUN Yan +7 位作者 ZHANG Ai-qin LIU Jia-sen LU Yan LIU Pei-xin YUAN Dong-wei JIANG Qian SI Chang-de QU Lian-dong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2014年第10期2268-2275,共8页
Pasteurella multocida, a Gram-negative nonmotile coccobacillus, is the causative agent of fowl cholera, bovine hemorrhagic septicemia, enzoonotic pneumonia and swine atropic rhinitis. Two filamentous hemagglutinin gen... Pasteurella multocida, a Gram-negative nonmotile coccobacillus, is the causative agent of fowl cholera, bovine hemorrhagic septicemia, enzoonotic pneumonia and swine atropic rhinitis. Two filamentous hemagglutinin genes, fhaB1 and JhaB2, are the potential virulence factors. In this study, an inactivationfhaB1 mutant ofP. multocida in avian strain C48-102 was constructed by a kanamycin-resistance cassette. The virulence of thefhaB1 mutant and the wild type strain was assessed in chickens by intranasal and intramuscular challenge. The inactivation offhaB1 resulted in a high degree of attenuation when the chickens were challenged intranasally and a lesser degree when challenged intramuscularly. ThefhaB1 mutant and the wild type strain were investigated their sensitivity to the antibody-dependent classical complement-mediated killing pathway in 90% convalescent chicken serum. ThefhaB1 mutant was serum sensitive as the viability has reduced between untreated serum and heat inactivated chicken serum (P〈0.007). These results confirmed that FhaB1 played the critical roles in the bacterial pathogenesis and further studies were needed to investigate the mechanism which caused reduced virulence of the fhaB1 mutant. 展开更多
关键词 Pasteurella multocida filamentous hemagglutinin B 1 PATHOGENICITY VIRULENCE
下载PDF
鹅巴氏杆菌油佐剂灭活疫苗的研制 被引量:2
7
作者 王学理 鄢长庆 +2 位作者 刘珂飞 杨明 杨明霞 《湖北农业科学》 北大核心 2012年第5期965-967,共3页
为了有效控制霍尔多巴吉鹅巴氏杆菌病(Pasteurellosis)的发生,将内蒙古通辽地区分离鉴定的巴氏杆菌作为菌种,采用液体增菌培养后灭活,加入佐剂制成油乳剂灭活疫苗,经无菌和安全检验合格后免疫20日龄雏鹅(0.5 mL/只)。结果表明,该疫苗免... 为了有效控制霍尔多巴吉鹅巴氏杆菌病(Pasteurellosis)的发生,将内蒙古通辽地区分离鉴定的巴氏杆菌作为菌种,采用液体增菌培养后灭活,加入佐剂制成油乳剂灭活疫苗,经无菌和安全检验合格后免疫20日龄雏鹅(0.5 mL/只)。结果表明,该疫苗免疫效果良好,1次免疫后保护率达73.3%,2次免疫后保护率达96.7%。 展开更多
关键词 巴氏杆菌(Pasteurella multocida) 霍尔多巴吉鹅 疫苗
下载PDF
猪呼吸道病综合征的流行与防控 被引量:4
8
作者 翁善钢 《养猪》 2017年第2期126-128,共3页
过去的20年间,有一种被称作猪呼吸道病综合征(porcine respiratory disease complex,PRDC)的疾病先后在北美和欧洲流行。该综合征包括了几种可以独立发生的疾病。不过,从病原学的角度往往是以几种病原混合感染的形式出现。根据不同学... 过去的20年间,有一种被称作猪呼吸道病综合征(porcine respiratory disease complex,PRDC)的疾病先后在北美和欧洲流行。该综合征包括了几种可以独立发生的疾病。不过,从病原学的角度往往是以几种病原混合感染的形式出现。根据不同学者的研究,该病的可能病原至少有3种类型, 展开更多
关键词 猪呼吸道病 猪肺 混合感染 porcine 支原体肺炎 多杀性巴氏杆菌 PMWS multocida 猪霍乱沙门氏菌 PASTEURELLA
下载PDF
Development of a SYBR-based real-time PCR to detect rabbit hemorrhagic disease virus(RHDV) and analyze its tissue distribution in experimentally infected rabbits 被引量:4
9
作者 Wenkai Liu +3 位作者 Ruyi Dang Xinglong Wang 《Virologica Sinica》 SCIE CAS CSCD 2015年第3期228-230,共3页
Dear Editor,Rabbit hemorrhagic disease(RHD)is a highly contagious disease of both wild and domesticated rabbits(Oryctolagus cuniculus).The causative agent of the disease is the rabbit hemorrhagic disease virus(RHDV),b... Dear Editor,Rabbit hemorrhagic disease(RHD)is a highly contagious disease of both wild and domesticated rabbits(Oryctolagus cuniculus).The causative agent of the disease is the rabbit hemorrhagic disease virus(RHDV),belongs to the genus Lagovirus within the family Caliciviridae(Granzow et al.,1996;Ohlinger et al.,1990).It is a small and non-enveloped virus with a 7.5 kb single stranded positive sense RNA genome(Meyers et al.,1991;Meyers et al.,2000).Based on an analysis of VP60 展开更多
关键词 HEMORRHAGIC RABBIT contagious belongs stranded INFECTED RABBIT PLASMID organs multocida
原文传递
四种家兔呼吸道疾病病原多重PCR方法的建立与应用 被引量:4
10
作者 邓钊宾 陈铭 +4 位作者 耿毅 杨泽晓 坤清芳 汪开毓 李亚军 《中国兽医科学》 CAS CSCD 北大核心 2016年第3期275-281,共7页
为建立同时检测家兔肺炎克雷伯菌(K.pneum oniae)、兔病毒性出血症病毒(RH D V)、多杀性巴氏杆菌(P.m ultocida)和支气管败血波氏杆菌(B.bronchiseptica)的多重PC R方法,根据G en Bank中肺炎克雷伯菌的16S r RN A基因、RHDV的VP60基因... 为建立同时检测家兔肺炎克雷伯菌(K.pneum oniae)、兔病毒性出血症病毒(RH D V)、多杀性巴氏杆菌(P.m ultocida)和支气管败血波氏杆菌(B.bronchiseptica)的多重PC R方法,根据G en Bank中肺炎克雷伯菌的16S r RN A基因、RHDV的VP60基因、多杀性巴氏杆菌的ktm1基因和支气管败血波氏杆菌的ptx A基因的保守序列设计4对特异性引物,优化反应体系与反应条件,建立能同时扩增兔肺炎克雷伯菌(127bp)、RHDV(322 bp)、多杀性巴氏杆菌(457 bp)和支气管败血波氏杆菌(872 bp)相应基因的四重PCR方法。结果显示,该方法能单管同时特异性检测兔源肺炎克雷伯菌、RHDV、多杀性巴氏杆菌与支气管败血波氏杆菌,且对兔源金黄色葡萄球菌、无乳链球菌、大肠杆菌与魏氏梭菌等无特异性扩增,说明该方法具有较强的特异性。该方法的灵敏度分别为9.16、7.24×10-3、7.96、6.8×10-1pg/L。采用建立的方法对人工感染试验病例与临床样本检测结果表明,该方法能快速、特异地鉴别诊断4种病原的单独与混合感染,与单一PCR方法相比,无显著性差异(P>0.05)。该方法的建立为临床上鉴别诊断4种家兔呼吸道疾病病原提供了重要的技术手段。 展开更多
关键词 兔病毒性出血症病毒 多杀性巴氏杆菌 支气管败血波氏杆菌 肺炎克雷伯菌 多重PCR 家兔
原文传递
Subcutaneous abscess caused by Pasteurella multocida in a patient due to a cat bite 被引量:1
11
作者 ZONGZhi-yong GAOYan-yu WANGXiao-hui 《Chinese Medical Journal》 SCIE CAS CSCD 2005年第12期1045-1046,共2页
P asteurella multocida (P. multocida), a Gram-negative pathogen, is mainly associated with animal infections. Human P. multocida infections are occasionally encountered and most human cases are due to the anima... P asteurella multocida (P. multocida), a Gram-negative pathogen, is mainly associated with animal infections. Human P. multocida infections are occasionally encountered and most human cases are due to the animal exposure or contact, especially due to cat bites. 1 In China, P. multocida infections are rarely described. CASE REPORT A 50-year old woman was sent to the Emergency Department of West China Hospital on June 25, 2004, due to the complain of an onset of pain, erythema, bleeding, and swelling at a wound on the right leg, caused by a pet cat bite 11 hours ago. She was diagnosed as having a wound infection and received intravenous penicillin G 4 million U/day. One day later, though the bleeding was ceased, she felt worse. Then, she was admitted to the Department of Infectious Diseases. She has no fever nor other systemic symptoms when she was admitted. On examination, three close, small and deep holes were found on the right leg near the ankle. Around the holes, the skin and soft tissue were red swelling and tenderness with a high skin temperature. Little pus was presented when the wound was crushed. Regional lymphadenopathy in right groin was found. No dyskinesia and tenderness of the ankle joint was found. Examination of her respiratory, cardiovascular, and gastrointestinal systems was unremarkable. Initial blood routine examination for her revealed: haemoglobin 12.5 g/dl; white blood cell count 7×10 9/L (neutrophils 4.48, lymphocytes 2.45); platelet, 184×10 9/L. But the white cell count increased to 11.2×10 9/L (neutrophils 8.52, lymphocytes 2.58) on the next day. Routine biochemical examination for her revealed normality. 展开更多
关键词 Pasteurella multocida ABSCESS BITE
原文传递
Presence of <i>Actinobacillus pleuropneumoniae, Streptococcus suis, Pasteurella multocida, Bordetella bronchiseptica, Haemophilus parasuis and Mycoplasma hyopneumoniae</i>in upper respiratory tract of swine in farms from Aguascalientes, Mexico 被引量:1
12
作者 Abraham Loera-Muro Francisco J. Avelar-González +2 位作者 Víctor M. Loera-Muro Mario Jacques Alma L. Guerrero-Barrera 《Open Journal of Animal Sciences》 2013年第2期132-137,共6页
Respiratory diseases are one of the most important health problems in pig herds. The porcine respiratory disease complex (PRDC) is the term used to describe pneumonic diseases caused by multiple infectious agents that... Respiratory diseases are one of the most important health problems in pig herds. The porcine respiratory disease complex (PRDC) is the term used to describe pneumonic diseases caused by multiple infectious agents that provoke weight loss in animals or death. In the PRDC multiple pathogens (bacteria and/or viruses) work in combination to induce this respiratory disease. Within this complex, Actinobacillus pleuropneumoniae, Streptococcus suis, Pasteurella multocida, Bordetella bronchiseptica, Haemophilus parasuis and Mycoplasma hyopneumoniae are the main bacterial pathogens involved in great economic losses to the swine industry. The aim of this work was to estimate the presence of A. pleuropneumoniae, S. suis, P. multocida, B. bronchiseptica, H. parasuis and M. hyopneumoniae in the upper respiratory tract of pigs in representative swine farms inAguascalientes,Mexico, using PCR technique. The study was performed in 14 swine farms. We obtained a total of 212 nasal swabs. Near 20% of samples were positive for A. pleuropneumoniae (located in the 79% of farms);17% were positive for S. suis (in 86% of farms), of these, 3% were S. suis serovar 2;30% were positive for H. parasuis (93% of farms);23% of the samples to P. multocida (in 79% of farms);and 19% to M. hyopneumoniae (in 64% of farms). B. bronchiseptica was not detected in this study. The results obtained show that bacterial pathogens of PRDC were present in the upper respiratory tract of pigs in all farms studied;therefore, these pathogens are widely disseminated in pig farms of Aguascalientes, Mexico. 展开更多
关键词 Porcine Respiratory Disease Complex ACTINOBACILLUS pleuropneumoniae Streptococcus SUIS PASTEURELLA multocida BORDETELLA bronchiseptica HAEMOPHILUS parasuis Mycoplasma hyopneumoniae
下载PDF
禽多杀性巴氏杆菌PCR检测方法的初步建立 被引量:3
13
作者 闫瑞雪 罗青平 +3 位作者 汪辉 董俊 孔令严 刘国平 《湖北畜牧兽医》 2015年第2期5-7,共3页
禽多杀性巴氏杆菌(Pasteurella multocida,PM)是引起禽霍乱的一种病原菌,能快速引起家禽的败血症等一系列病症,给我国的家禽业造成了巨大的经济损失。试验通过在Gene Bank中查询禽多杀性巴氏杆菌的全基因组序列,从而找到PM的保守序列,运... 禽多杀性巴氏杆菌(Pasteurella multocida,PM)是引起禽霍乱的一种病原菌,能快速引起家禽的败血症等一系列病症,给我国的家禽业造成了巨大的经济损失。试验通过在Gene Bank中查询禽多杀性巴氏杆菌的全基因组序列,从而找到PM的保守序列,运用Primer5设计引物,常规PCR后得到目的核酸扩增产物。经过敏感性、特异性、干扰性等试验确定了该方法的特异性和稳定性。结果证明该方法特异性强,敏感性高,干扰性极低,并且与生化鉴定得出的结果有较高的符合率。该方法简单易操作,适合广泛应用于临床以检测禽多杀性巴氏杆菌病。 展开更多
关键词 禽多杀性巴氏杆菌(Pasteurella multocida PM) PCR检测 敏感性 特异性 干扰性
下载PDF
A large-scale fatal outbreak of Pasteurella multocida among wild rodents in southwestern China 被引量:3
14
作者 Chun-Hong Du Yong Huang +17 位作者 Jia-Fu Jiang Zi-Hou Gao Na Jia Shan Yang Chao-Nian Chen Shun-Gao Ma Yu-Ying Liang Bao-Gui Jiang Wei-Hong Sang Peng Wang Yun Liang Yun-Zhi Zhang Ji-Hua Zhou Shui-Ping Chen Frans Jongejan Zhi-Zhong Song Yi-Gang Tong Wu-Chun Cao 《Journal of Biosafety and Biosecurity》 2020年第2期91-98,共8页
Background:Pasteurella multocida is an important and old zoonotic pathogen worldwide which has an impressive host spectrum including numerous domestic and wild animals as well as birds,causing specific diseases or out... Background:Pasteurella multocida is an important and old zoonotic pathogen worldwide which has an impressive host spectrum including numerous domestic and wild animals as well as birds,causing specific diseases or outbreak with great economic impact.It has never been reported that P.multocida can cause an epidemic in wild rodents.In June 5–17,2016,more than 1000 rodent deaths of an unknown cause quickly spread in the PuEr City,Yunnan province,southwestern China.Methods:The rodents in affected areas and outside of the epidemic areas were collected and screened for possible known pathogens including Yersinia pestis,rabies virus and hantavirus as well as other bacteria.The possible bacterial pathogens were isolated both by culture medium and by mouse inoculation in parallel.The isolates were identified by the Vitek GNI card and PCR assays for 16S rRNA genes.The pathogen strains were selected for whole genome sequencing analysis.Results:A total of 123 rodents were collected from 25 sample sites at affected area,among of which,all 119 dead rodents were negative for the pathogen under consideration except P.multocida,and all four live rodents were negative for P.multocida.In addition,480 rodents collected from other 23 counties outside of the epidemic area in Yunnan were negative for with P.multocida.A total of 14 strains of P.multocida(six directly isolated from the field rodents and eight from the experimental mice that were injected with the organ substrates from the dead rodents)belonged to serogroup A and serogroup F represented by 9 N and 20 N were identified in these epidemic areas.Whole genome sequencing revealed that the serogroup F strain shared 99%similarity to P.multocida Pm70 from chicken,but contained a 50 k bp insertion sequence.The serogroup A strain shared 95%similarity to P.multocida FDAARGOS_385 from a human patient,but contained four large structural differences.Histological abnormalities were identified in the livers,lungs,hearts and brains of the inoculated mice.Conclusions:The simultaneous occu 展开更多
关键词 Pasteurella multocida Serotypes A Serotypes F RODENTS OUTBREAK China
原文传递
多杀性巴氏杆菌外源基因表达元件的构建及其序列分析
15
作者 李国攀 熊萍萍 荣俊 《湖北农业科学》 2015年第19期4847-4851,共5页
为了在多杀性巴氏杆菌(Pasteurella multocida)中高效表达外源基因,克隆了天冬酰氨合成酶A(asn A)基因的启动子Pasn A和终止子Tasn A,双酶切获取p UC18质粒的多克隆位点,将这3个片段组装成一个可以方便插入外源基因的表达元件。测序结... 为了在多杀性巴氏杆菌(Pasteurella multocida)中高效表达外源基因,克隆了天冬酰氨合成酶A(asn A)基因的启动子Pasn A和终止子Tasn A,双酶切获取p UC18质粒的多克隆位点,将这3个片段组装成一个可以方便插入外源基因的表达元件。测序结果分析表明,Pasn A是巴氏杆菌asn A基因的强启动子,具有原核生物典型的Pribnow盒和SD序列。p UC18的多克隆位点mcs具有多个核酸限制性内切酶的单一切点,便于外源基因的插入。Tasn A是巴氏杆菌asn A基因的强终止子,具有很好的回文结构,在发卡结构后面有一段富含A/T区。3个片段表达元件(Pcms T)的构建为完整的巴氏杆菌-大肠杆菌穿梭表达质粒的构建奠定了基础。 展开更多
关键词 多杀性巴氏杆菌(Pasteurella multocida) 表达元件 天冬酰氨合成酶A基因 启动子 终止子
下载PDF
Adjuvant activity of Pasteurella multocida A strain,Pasteurella multocida B strain and Salmonella typhimurium bacterial DNA on cellular and humoral immunity responses against Pasteurella multocida specific strain infections in Balb/c mice
16
作者 Maryam Homayoon Yahya Tahamtan +2 位作者 Mohammad Kargar Seyed Mohammad Hossein Hosseini Abbas Akhavan Sepahy 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2018年第5期336-341,共6页
Objective: To evaluate the effects of Pasteurella multocida(P. multocida) vaccines on the expression and release of antibodies, interleukin(IL)-6 and IL-12 by serum. Methods: Balb/c mice were immunized with two formal... Objective: To evaluate the effects of Pasteurella multocida(P. multocida) vaccines on the expression and release of antibodies, interleukin(IL)-6 and IL-12 by serum. Methods: Balb/c mice were immunized with two formalin and iron inactivated vaccine doses within 2 weeks. The vaccines were adjuvant with P. multocida A strain, P. multocida B strain and Salmonella typhimurium bacterial DNA(AbDNA, BbDNA and SbDNA for short, respectively). The animals were challenged 4 weeks after immunization. Blood of mice was collected to detect the change of specific antibody, IL-6, and IL-12 using ELISA. Results: The specific antibody and interleukins in the immunized group increased significantly compared to the control mice after vaccination and challenge(P<0.05). The highest release of these cytokines was obtained by P.multocida inactivated with iron and adjuvant with AbDNA at a concentration of 25 μg/mL. The antibody titer peak was 0.447 in mice vaccinated with iron-killed whole-cell antigen adjunct with AbDNA. The time-courses of release showed that bacterial DNA was able to stimulate IL-6 and IL-12 production more than alum(P<0.05). Conclusions: Our findings introduce that bacterial DNA is capable of releasing an immunological response with several cytokines.These indicate that bacterial DNA entrapped with killed P. multocida antigen is a new and effective adjuvant to enhance specific immunity and resistance of animal against the infectious pathogen, which could simplify the development of highly promising strong adjuvant. 展开更多
关键词 Pasteurella multocida Bacterial DNA Adjuvant activity VACCINE IMMUNITY Balb/c mice
下载PDF
Deadly case of Pasteurella multocida aortitis and mycotic aneurysm following a cat bite
17
作者 Dennis Dane Cho Yaniv Berliner David Carr 《World Journal of Clinical Cases》 SCIE 2016年第6期142-145,共4页
Animal bites are frequently encountered in the emergency department(ED). Aortitis leading to mycotic abdominal aortic aneurysm is a rare and potentially deadly complication of Pasteurella multocida(P. multocida) follo... Animal bites are frequently encountered in the emergency department(ED). Aortitis leading to mycotic abdominal aortic aneurysm is a rare and potentially deadly complication of Pasteurella multocida(P. multocida) following an animal bite. We present the case of a 68-year-old male who presented to the ED after falling at home. He complained of weakness and abdominal pain. He was in septic shock and was treated empirically with broad-spectrum antibiotics and intravenous fluids. He reported previous antibiotic treatment of a cellulitis secondary to a cat bite injury to his right thumb four weeks prior. Abdominal ultrasound and subsequent computed tomography scan revealed a leaking mycotic abdominal aneurysm that was surgically repaired. Blood cultures and aortic wall tissue cultures grew P. multocida. Given how common animal bite presentations are in the ED, this case highlights the need to consider aortitis and mycotic abdominal aortic aneurysm in an unwell patient with an animal bite. 展开更多
关键词 Mycotic aneurysm Emergency department Cat bite Pasteurel a multocida AORTITIS
下载PDF
Molecular analysis of Pasteurella multocida strains isolated from fowl cholera infection in backyard chickens
18
作者 Mohamed-Wael Abdelazeem Mohamed Moemen Abdel Azeem Mohamed Abdel Mageed 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2014年第1期8-12,共5页
Objective:To characterize Pasteurella isolated from backyard chickens using whole cell protein lysate profiles and random amplified polymorphic DNA(RAPD)techniques to show their genetic relationship because Pasteurcll... Objective:To characterize Pasteurella isolated from backyard chickens using whole cell protein lysate profiles and random amplified polymorphic DNA(RAPD)techniques to show their genetic relationship because Pasteurclla multocida(P.multocida)is an important cause of fatal infections in backyard chickens.Methods:Twenty one P.multocida isolates were recovered previously from clinical cases of fowl cholera belonging to individual owners and phenotypically analysed using biochemical tests and serotyping were used far the genetic characterization.Results:Phylogenetic study based on both methods revealed that the recovered population of P.multocida isolated from backyard chickens differs markedly,constituting a well-separated cluster and appearance of 3 distinguishing lineages with greater discrimination shown by RAPDPCR that resulted in two suclusters in cluster A and three subclusters in cluster B and were related greatly with capsular serogroups for the examined strains.The whole cell protein revealed the presence of dominant protein bands at approximately 41 and 61 kDa in all of the examined isolates that may be a virulent proteins share in the increasing of its pathogenicity.Clear distinctive bands ranged from 123 to 1534 bp.Conclusions:Based on the previous findings,there are three spreading clusters that may indicate the association of a small number of P.multocida variants with the majority of cases suggesting that certain clones of P.multocida are able to colonue the examined backyard chickens.Also,the ease and rapidity of RAPD-PCR support the use of this technique as alternative to the more labour-intensive SDS-PACE system for strain differentiation and epidemiological studies of avian P.multocida.Further application of RAPD technology to the examination of avian cholera outbreaks in commercially available flocks may facilitate more effective management of this disease by providing the potential to investigate correlations of P.multocida genotypes,to identify affiliations between bird types and bacterial genot 展开更多
关键词 PASTEURELLA multocida CHICKENS Variation Molecular characterization
下载PDF
Study on Genetic Inactivation Bacterial Ghosts of Pasteurella multocida based PhiX174 Gene E Lysis Cassette Mediated
19
作者 ZHU Bi-feng YANG Xu-fu LIU Dong-sheng 《Animal Husbandry and Feed Science》 CAS 2012年第4期172-175,共4页
[Objective] To study on genetic inactivation bacterial ghosts of Pasteurella multocida based PhiX174 gene E lysis cassette mediated. [ Method ] Recombinant pPBA1100-e was constructed by which the gene E of bacteriopha... [Objective] To study on genetic inactivation bacterial ghosts of Pasteurella multocida based PhiX174 gene E lysis cassette mediated. [ Method ] Recombinant pPBA1100-e was constructed by which the gene E of bacteriophage Phix174 and temperature sensitivity expressing control system hybridized with plasmid pPBA1100 by genetic engineering method. Recombinant was transformed to Pasteurella multocida and lysis gene E expressed by temperature induction. Recombinant was detected by restriction endonuclease. Cell morphology of bacterial ghost of Pasteurella mul- tocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysis. I Result~ The results indicated that the recombination plasmid presented three bands by restriction endonuclease and agarose electrophoresis and that molecular weight of every band ac- corded with theoretical value. The result of SEM observing showed that recombination plasmid expressed successfully in P. multocida and produced bacterial ghost. The result of CFU detecting demonstrated that inactivation ratio of P. multocida reached 99 per cent. ~Conclusion~ This study pro- vided technical bases for the preparation of antigen vaccine of natural bacterial outer membrane protein. 展开更多
关键词 lysis gene E P. multocida Bacterial ghost Plasmid construction
下载PDF
多杀性巴氏杆菌相关毒力因子的研究进展 被引量:2
20
作者 四郎曲珍 《中国畜牧兽医文摘》 2015年第9期66-66,228,共2页
多杀性巴氏杆菌(pasteurella multocida,Pm)是重要的畜禽致病菌,可引起多种动物出现巴氏杆菌病。家禽感染表现为禽霍乱,猪感染则引起萎缩性鼻炎,牛,羊感染则主要表现为出血性败血症和传染性肺炎。此外,还能感染美洲野牛,牦牛,鹿,河马... 多杀性巴氏杆菌(pasteurella multocida,Pm)是重要的畜禽致病菌,可引起多种动物出现巴氏杆菌病。家禽感染表现为禽霍乱,猪感染则引起萎缩性鼻炎,牛,羊感染则主要表现为出血性败血症和传染性肺炎。此外,还能感染美洲野牛,牦牛,鹿,河马,大象,雪豹及狮子等野生动物。 展开更多
关键词 多杀性巴氏杆菌 毒力因子 出血性败血症 萎缩性鼻炎 multocida 美洲野牛 外膜蛋白 牛出血性败血病 流行区 宿主细胞
下载PDF
上一页 1 2 33 下一页 到第
使用帮助 返回顶部