Our aim is to evaluate the association between body mass index(BMI)and preoperative total testosterone(TT)levels with the risk of single and multiple metastatic lymph node invasion(LNI)in prostate cancer patients unde...Our aim is to evaluate the association between body mass index(BMI)and preoperative total testosterone(TT)levels with the risk of single and multiple metastatic lymph node invasion(LNI)in prostate cancer patients undergoing radical prostatectomy and extended pelvic lymph node dissection.Preoperative BMI,basal levels of TT,and prostate-specific antigen(PSA)were evaluated in 361 consecutive patients undergoing radical prostatectomy with extended pelvic lymph node dissection between 2014 and 2017・Patients were grouped into either nonmetastatic,one,or more than one metastatic lymph node invasion groups.The association among clinical factors and LNI was evaluated.LNI was detected in 52(14.4%)patients:28(7.8%)cases had one metastatic node and 24(6.6%)had more than one metastatic node.In the overall study population,BMI correlated inversely with TT(r=-0.256;P<0.0001).In patients without metastases,BMI inversely correlated with TT(r=-0.282;P<0.0001).In patients with metastasis,this correlation was lost.In the overall study population,BMI(odds ratio[OR]=1.268;P=0.005)was the only in dependent clinical factor associated with the risk of multiple metastatic LNI compared to cases with one metastatic node.In the nonmetastatic group,TT was lower in patients with BMI>28 kg m^2(P<0.0001).In patients with any LNI,this association was lost(P=0.232).The median number of positive nodes was higher in patients with BMI>28 kg m^2(P-0.048).In our study,overweight and obese patients had a higher risk of harboring multiple prostate cancer lymph node metastases and lower TT levels when compared to patients with normal BMI.展开更多
目的探讨沉默核蛋白1(nuclear protein 1,NUPR1)对人多发性骨髓瘤细胞自噬、迁移和侵袭的影响及其机制。方法用含有NUPR1-shRNA慢病毒载体的病毒感染骨髓瘤U266细胞,通过流式细胞术检测转染效率,实验分为阴性对照组(转染无关序列shRNA...目的探讨沉默核蛋白1(nuclear protein 1,NUPR1)对人多发性骨髓瘤细胞自噬、迁移和侵袭的影响及其机制。方法用含有NUPR1-shRNA慢病毒载体的病毒感染骨髓瘤U266细胞,通过流式细胞术检测转染效率,实验分为阴性对照组(转染无关序列shRNA慢病毒)和敲低组(转染NUPR1-shRNA慢病毒)。qRT-PCR检测NUPR1mRNA表达,Western blot检测NUPR1、自噬相关蛋白(LC3Ⅱ/LC3Ⅰ、Beclin1、ATG5和P62)、迁移和侵袭相关蛋白(MMP9、CXCR4)以及通路蛋白(p-AKT/T-AKT和p-mTOR/T-mTOR)的表达;透射电子显微镜(TEM)观察自噬小体;Transwell检测细胞迁移和侵袭能力。结果与阴性对照组比较,敲低组NUPR1 mRNA和蛋白表达显著下调(P<0.05),自噬相关蛋白ATG5、Beclin1和LC3Ⅱ/LC3Ⅰ的表达明显降低(P<0.05),而MMP9、CXCR4、P62、p-AKT/T-AKT和p-mTOR/T-mTOR蛋白表达明显增高(P<0.05);敲低组自噬小体明显减少,细胞迁移和侵袭能力增强,雷帕霉素可阻断该促进作用。结论沉默NUPR1可能通过降低自噬水平促进U266细胞迁移和侵袭,并可能与AKT/mTOR通路激活有关。展开更多
[目的]检测miR-410与HMGB1在多发性骨髓瘤中的表达水平及其对细胞增殖与侵袭能力的影响,探讨miR-410在多发性骨髓瘤致病中的作用。[方法]实时荧光定量PCR检测多发性骨髓瘤骨髓及细胞中miR-410的表达水平;蛋白质印迹用于分析HMGB1蛋白表...[目的]检测miR-410与HMGB1在多发性骨髓瘤中的表达水平及其对细胞增殖与侵袭能力的影响,探讨miR-410在多发性骨髓瘤致病中的作用。[方法]实时荧光定量PCR检测多发性骨髓瘤骨髓及细胞中miR-410的表达水平;蛋白质印迹用于分析HMGB1蛋白表达水平;CCK8实验和Boyden侵袭小室实验分别用于检测细胞增殖活性及侵袭能力;双荧光素酶报告实验用于检测miR-410与HMGB1之间的相互作用;通过重组质粒构建及细胞转染调节相关基因在细胞内的表达;通过建立裸鼠异位移植瘤模型,在体验证miR-410对肿瘤生长的影响。[结果] Mi R-410在多发性骨髓瘤骨髓及细胞中表达均下调(P<0.01),而HMGB1表达均上调(P<0.01)。Mi R-410抑制多发性骨髓瘤细胞的增殖和侵袭(P<0.01)。Mi R-410通过与HMGB1的3’UTR作用,负向调控HMGB1的表达。Mi R-410通过下调HMGB1抑制多发性骨髓瘤细胞增殖和侵袭(P<0.05)。Mi R-410抑制多发性骨髓瘤移植瘤小鼠肿瘤生长(P<0.05)。[结论] Mi R-410通过负向调控HMGB1表达,抑制多发性骨髓瘤细胞增殖和侵袭,从而抑制肿瘤发生发展。展开更多
文摘Our aim is to evaluate the association between body mass index(BMI)and preoperative total testosterone(TT)levels with the risk of single and multiple metastatic lymph node invasion(LNI)in prostate cancer patients undergoing radical prostatectomy and extended pelvic lymph node dissection.Preoperative BMI,basal levels of TT,and prostate-specific antigen(PSA)were evaluated in 361 consecutive patients undergoing radical prostatectomy with extended pelvic lymph node dissection between 2014 and 2017・Patients were grouped into either nonmetastatic,one,or more than one metastatic lymph node invasion groups.The association among clinical factors and LNI was evaluated.LNI was detected in 52(14.4%)patients:28(7.8%)cases had one metastatic node and 24(6.6%)had more than one metastatic node.In the overall study population,BMI correlated inversely with TT(r=-0.256;P<0.0001).In patients without metastases,BMI inversely correlated with TT(r=-0.282;P<0.0001).In patients with metastasis,this correlation was lost.In the overall study population,BMI(odds ratio[OR]=1.268;P=0.005)was the only in dependent clinical factor associated with the risk of multiple metastatic LNI compared to cases with one metastatic node.In the nonmetastatic group,TT was lower in patients with BMI>28 kg m^2(P<0.0001).In patients with any LNI,this association was lost(P=0.232).The median number of positive nodes was higher in patients with BMI>28 kg m^2(P-0.048).In our study,overweight and obese patients had a higher risk of harboring multiple prostate cancer lymph node metastases and lower TT levels when compared to patients with normal BMI.
文摘目的探讨沉默核蛋白1(nuclear protein 1,NUPR1)对人多发性骨髓瘤细胞自噬、迁移和侵袭的影响及其机制。方法用含有NUPR1-shRNA慢病毒载体的病毒感染骨髓瘤U266细胞,通过流式细胞术检测转染效率,实验分为阴性对照组(转染无关序列shRNA慢病毒)和敲低组(转染NUPR1-shRNA慢病毒)。qRT-PCR检测NUPR1mRNA表达,Western blot检测NUPR1、自噬相关蛋白(LC3Ⅱ/LC3Ⅰ、Beclin1、ATG5和P62)、迁移和侵袭相关蛋白(MMP9、CXCR4)以及通路蛋白(p-AKT/T-AKT和p-mTOR/T-mTOR)的表达;透射电子显微镜(TEM)观察自噬小体;Transwell检测细胞迁移和侵袭能力。结果与阴性对照组比较,敲低组NUPR1 mRNA和蛋白表达显著下调(P<0.05),自噬相关蛋白ATG5、Beclin1和LC3Ⅱ/LC3Ⅰ的表达明显降低(P<0.05),而MMP9、CXCR4、P62、p-AKT/T-AKT和p-mTOR/T-mTOR蛋白表达明显增高(P<0.05);敲低组自噬小体明显减少,细胞迁移和侵袭能力增强,雷帕霉素可阻断该促进作用。结论沉默NUPR1可能通过降低自噬水平促进U266细胞迁移和侵袭,并可能与AKT/mTOR通路激活有关。
文摘[目的]检测miR-410与HMGB1在多发性骨髓瘤中的表达水平及其对细胞增殖与侵袭能力的影响,探讨miR-410在多发性骨髓瘤致病中的作用。[方法]实时荧光定量PCR检测多发性骨髓瘤骨髓及细胞中miR-410的表达水平;蛋白质印迹用于分析HMGB1蛋白表达水平;CCK8实验和Boyden侵袭小室实验分别用于检测细胞增殖活性及侵袭能力;双荧光素酶报告实验用于检测miR-410与HMGB1之间的相互作用;通过重组质粒构建及细胞转染调节相关基因在细胞内的表达;通过建立裸鼠异位移植瘤模型,在体验证miR-410对肿瘤生长的影响。[结果] Mi R-410在多发性骨髓瘤骨髓及细胞中表达均下调(P<0.01),而HMGB1表达均上调(P<0.01)。Mi R-410抑制多发性骨髓瘤细胞的增殖和侵袭(P<0.01)。Mi R-410通过与HMGB1的3’UTR作用,负向调控HMGB1的表达。Mi R-410通过下调HMGB1抑制多发性骨髓瘤细胞增殖和侵袭(P<0.05)。Mi R-410抑制多发性骨髓瘤移植瘤小鼠肿瘤生长(P<0.05)。[结论] Mi R-410通过负向调控HMGB1表达,抑制多发性骨髓瘤细胞增殖和侵袭,从而抑制肿瘤发生发展。
