AIM： To evaluate the antibacterial activity of Sapindus mukorossi （S. mukorossl） and Rheum emodi （R. emodl）. METHODS： Powders of S. mukorossi and R. emodi were extracted successively with petroleum ether, benzen...AIM： To evaluate the antibacterial activity of Sapindus mukorossi （S. mukorossl） and Rheum emodi （R. emodl）. METHODS： Powders of S. mukorossi and R. emodi were extracted successively with petroleum ether, benzene, chloroform and ethanol and were concentrated in vacuum. The disk diffusion method was used for in vitro studies and in vivo studies were performed on male Wister rats. Thirty resistant clinical isolates of H pylon, as determined by their antibiotic sensitivity patterns by E-test, along with two Gram ＋ve （S. aureus, B. subtilis） and two Gram -ve （E. coli, P. vugaris） organisms were screened for their susceptibility patterns against these extracts. RESULTS： In our screening, all 30 resistant isolates and the other four organisms （two Gram ＋ve S. aureus, B. subtilis and two Gram -ve, E. coli, P. vugaris） were sensitive to the test compounds. It was found that ethanol and chloroform extracts of S. mukorossi and ethanol and benzene extracts of R. emodi inhibited Hpylori at very low concentrations, In the in vitro study, the isolates showed a considerable zone of inhibition at very low concentrations （10 μg/mL） for both the extracts. In the in vivo study, the H pylori infection was cleared with minimal doses of extracts of S. mukorossi （2.5 mg/mL） and R. emodi （3.0 mg/mL） given orally for seven days. CONCLUSION： We can conclude from this study that the extracts of S. mukorossi and R. emodi inhibited the growth of pylori in vitro and, in in vivo studies, the H pylori infection cleared within seven days at very low concentrations. We also found that H pylori did not acquire resistance against these herbal extracts even after 10 consecutive passages.展开更多
Objective:To investigate the antihyperglycemic and antihyperlipidemie properties of hydroalcoholic extract of fruits of Sapindus mukorossi Caerlen and its beneficial effect on haeniatological parameters with histopath...Objective:To investigate the antihyperglycemic and antihyperlipidemie properties of hydroalcoholic extract of fruits of Sapindus mukorossi Caerlen and its beneficial effect on haeniatological parameters with histopathological analysis in streptpzotocin induced diabetic rats.Methods:Sapindus mukorossi fruits extract(250 and 500 mg/kg body weight) and standard drug glybenclamide(0.5 mg/kg body weight) were administered to diabetic rats.Effect of extract on hyperglycemia,hyperlipidemia and hematological parameters was studied in diabetic rats.Histopathological changes in diabetic rat pancreas were also observed after extract and glybenclamide treatment.Results:Daily oral administration of Sapindus mukorossi fruits extract(250 and 500 mg/kg body weight) and glybenclamide for 20 days showed beneficial effects on blood glucose level(P【0.01) and lipid level.The extract has a favorable effect on the histopathological changes of the pancreas in streptozotocin induced diabetes.Conclusions: These findings reveal that the hydroalcoholic extract of Sapindus mukorossi fruits extract possesses antihyperglycemic and antihyperlipidemie properties.In addition.the extract can prevent various complications of diabetes and improve some haeniatological parameters.展开更多
Aim: To observe the alterations in the biochemical and biophysical changes in the sperm membrane during sperm maturation in male rats treated with the water extract of the fruit pericarp of S. mukorossi. Methods: Adul...Aim: To observe the alterations in the biochemical and biophysical changes in the sperm membrane during sperm maturation in male rats treated with the water extract of the fruit pericarp of S. mukorossi. Methods: Adult male Sprague-Dawley rats were gavaged the aqueous extract of the fruit pericarp of S. mukorossi at a dose of 50 mg/kg/d for 45 days. On day 46, the sperm parameters were observed in different sections of the epididymis and the sperm superoxide dismutase and the lipid peroxidation was determined and compared with the controls. The testis and epididymis were routinely prepared for histological examination under the light microscope. Results: No significant differences in the sperm number and morphology were observed between the control and treated groups. However, a significant inhibition (P<0.05-0.01) of sperm motility in the caput, corpus and cauda regions of the epididymis was seen in the treated group. No significant histopathological changes were found in the testis and epididymis. The important finding was that in the treated animals, the spermatozoa showed an abnormal distribution of the superoxide dismutase activity, being minimum in the caput and maximum in the corpus, which was just opposite to that of the controls. Conclusion: The study provides a unique observation where the plant extract alters the sperm membrane physiology without change the testicular and epididymal morphology.展开更多
This study explores the possible application of a biodegradable plant based surfactant, obtained from Sapindus mukorossi, for washing low levels of arsenic （As） from an iron （Fe） rich soil. Natural association of...This study explores the possible application of a biodegradable plant based surfactant, obtained from Sapindus mukorossi, for washing low levels of arsenic （As） from an iron （Fe） rich soil. Natural association of As（Ⅴ） with Fe（Ⅲ） makes the process difficult. Soapnut solution was compared to anionic surfactant sodium dodecyl sulfate （SDS） in down-flow and a newly introduced suction mode for soil column washing. It was observed that soapnut attained up to 86% efficiency with respect to SDS in removing As. Full factorial design of experiment revealed a very good fit of data. The suction mode generated up to 83 kPa pressure inside column whilst down-flow mode generated a much higher pressure of 214 kPa, thus making the suction mode more efficient. Micellar solubilisation was found to be responsible for As desorption from the soil and it followed 1st order kinetics. Desorption rate coefficient of suction mode was found to be in the range of 0.005 to 0.01, much higher than down-flow mode values. Analysis of the PT-IR data suggested that the soapnut solution did not interact chemically with As, offering an option for reusing the surfactant. Soapnut can be considered as a soil washing agent for removing As even from soil with high Fe content.展开更多
The study has been carried out to investigate the effects of single factors such as solvents extraction temperature, times, solid-liquid ration, and the time of extractions on the yields of saponins present in the pul...The study has been carried out to investigate the effects of single factors such as solvents extraction temperature, times, solid-liquid ration, and the time of extractions on the yields of saponins present in the pulp of Sapindus mukorossi Gaerth. On this basis, an L9 orthogonal design of experiment was adopted to determine the optimal conditions for the extraction of saponins. The factors that influence the extraction of saponins are put in the order of extraction times, extraction time, solid-liquid ration, and the best combination is that the powder of the pulp is extracted with EtOH (solid-to-solvent ratio = 1:8, w/v) for three times at 60°C for 3 hours. Under these conditions, about 1.63 g saponins will be extracted from 10 g raw material. The stability test showed that the Sapindus mukuross saponins can maintain surface activity at water temperature (25°C - 40°C), pH (6.3 - 7.7) and water hardness (50 - 250 mg.L-1). It is proved that Sapindus mukuross saponins are quality non-ionic active agent.展开更多
Two new tirucallane-type triterpenoid saponins, Sapimukoside A (1) and Sapimukoside B (2), were isolated from the roots of Sapindus mukorossi Gaertn. On the basis of spectroscopic and chemical evidence, their structur...Two new tirucallane-type triterpenoid saponins, Sapimukoside A (1) and Sapimukoside B (2), were isolated from the roots of Sapindus mukorossi Gaertn. On the basis of spectroscopic and chemical evidence, their structures were elucidated to be 3-O-alpha-L-rhamnopyranosyl-(1-->2)-[alpha-L-arabinopyranosyl-(1-->3)]-beta-D-glucopyranosyl-21, 23R-epoxyl tirucall-7, 24R-diene-3beta, 21-diol (1) and 3-O-alpha-L-rhamnopyranosyl-(1-->6)-beta-D-glucopyranosyl-21, 23R-epoxyl tirucall-7, 24R-diene-3beta, 21-diol (2).展开更多
To explore differences in leaf morphology between Sapindus mukorossi and Sapindus delavayi,and how the environment might drive these differences,80 germplasm samples from the Sapindus germplasm nursery in Fujian Provi...To explore differences in leaf morphology between Sapindus mukorossi and Sapindus delavayi,and how the environment might drive these differences,80 germplasm samples from the Sapindus germplasm nursery in Fujian Province were selected.The study revealed a wide variation and diversity in 16 germplasm traits,both within and between species grown under the same conditions.On average,the relative contribution of intraspecific variability to total variability was more important(83%) than the relative contribution of interspecific variability(17%).PERMANOVA analysis showed differences in leaflet thickness,length,perimeter,length to width ratio,and leaf hairs or trichome density.Correlation analyses between leaf morphological traits and environmental variables indicated that leaves tended to be larger,longer,and thicker in wetter,warmer,and low-altitude conditions.Our analysis of the relationship between climate and leaf morphology revealed that S.mukorossi had a greater sensitivity to climate variation,particularly in response to mean temperatures of the coldest and warmest seasons,which led to differences in leaf traits and the distribution of the two species.These findings contribute to the understanding of leaf morphology variations in S.mukorossi and S.delavayi,and provide a basis for the collection of Sapindus germplasm resources,their cultivation and use to help address climate change.展开更多
[Objectives]To investigate the antimicrobial activity and mechanism for saponin from Sapindus mukorossi against Escherichia coli.[Methods]E.coli was used as the test bacteria,and the antimicrobial effect of Sapindus s...[Objectives]To investigate the antimicrobial activity and mechanism for saponin from Sapindus mukorossi against Escherichia coli.[Methods]E.coli was used as the test bacteria,and the antimicrobial effect of Sapindus saponin was studied by the minimum inhibitory concentration method.The antimicrobial mechanism of Sapindus saponin was studied in terms of growth curves,membrane potential,activity of cells,cell surface morphology and cell internal structure.[Results]The results indicate that Sapindus saponin can inhibit the growth of E.coli,the minimum inhibitory concentration is 5 mg/mL and has a significant inhibitory effect on the growth of E.coli.After treated with Sapindus saponin,the membrane potential of E.coli increased by 16.7,17.3 and 19.1 times after exposition to 0.5,1,2 MIC Sapindus saponin respectively.PI staining results show that cell viability decreased and permeability of cell membranes increased.The results of SEM and TEM further corroborate the membrane damage,the cell was damaged after exposition to Sapindus saponin.[Conclusions]The antimicrobial mechanism of Sapindus saponin were to changed membrane potential of cell,and damage the cell membrane structures.展开更多
In order to solve the problem of rapid propagation of Sapindus mukorossi Gaertn seedlings, research on the cutting propagation of S. mukorossi Gaertn was conducted. The results showed that the rooting rate of cuttings...In order to solve the problem of rapid propagation of Sapindus mukorossi Gaertn seedlings, research on the cutting propagation of S. mukorossi Gaertn was conducted. The results showed that the rooting rate of cuttings of the ten clones ranged from 30.9% to 83.0%. Among them, the rooting rate of cuttings of clones SM01 and SM06 was higher than that of other clones, and there were significant differences between different clones in the rooting rate of cuttings. The rooting rate of the semi-lignified shoots having two leaves could be improved significantly. The treatment of the cuttings by GGR6 solutions had significant effects on their rooting rate.展开更多
Objective:Due to the many negative properties of sodium hypochlorite used in current root canal treatment,interest in biocompatible natural agents is increasing day by day.The aim of this study was to evaluate whether...Objective:Due to the many negative properties of sodium hypochlorite used in current root canal treatment,interest in biocompatible natural agents is increasing day by day.The aim of this study was to evaluate whether various extract solutions of Sapindus mukorossi have dissolution effects on human pulp tissues.Methods:Primarily powder extracts were obtained by extracting fruit shells of S.mukorossi in different solvents(ethanol,methanol,buthanol and distilled water).The test solutions were prepared and randomly separated into six groups with 10 samples in each group:ethanol extract,methanol extract,butanol extract,distilled water extract of S.mukorossi,sodium hypochlorite(Na OCl)and the control group.Among these,S.mukorossi extracts were separated into two subgroups,depending on their concentration level(50μg/m L and 100μg/m L).The pulp tissues of freshly extracted human molars were used for dissolution test.The weights of the pulpal tissues were measured and recorded for two times after the samples were placed in the solutions.Statistical analysis for all descriptive statistics was performed using SPSS 22(P<0.05).Results:Our results showed that maximum percent yield of preparation was obtained in methanol extract of S.mukorossi.Among all of the groups,the best dissolution capacity was seen in the Na OCl group(positive control group).Among S.mukorossi groups,the best tissue solvent solution was found in SMM group at 50μg/m L and SMB group at 100μg/m L.Conclusion:The different extracts of S.mukorossi had a capacity to dissolve pulp tissue but this capacity was less than Na OCl.Therefore,further studies will enable the creation of a commercial solution for clinical use by increasing the effectiveness of S.mukorossi while combining it with other endodontic irrigation solutions.展开更多
文摘AIM： To evaluate the antibacterial activity of Sapindus mukorossi （S. mukorossl） and Rheum emodi （R. emodl）. METHODS： Powders of S. mukorossi and R. emodi were extracted successively with petroleum ether, benzene, chloroform and ethanol and were concentrated in vacuum. The disk diffusion method was used for in vitro studies and in vivo studies were performed on male Wister rats. Thirty resistant clinical isolates of H pylon, as determined by their antibiotic sensitivity patterns by E-test, along with two Gram ＋ve （S. aureus, B. subtilis） and two Gram -ve （E. coli, P. vugaris） organisms were screened for their susceptibility patterns against these extracts. RESULTS： In our screening, all 30 resistant isolates and the other four organisms （two Gram ＋ve S. aureus, B. subtilis and two Gram -ve, E. coli, P. vugaris） were sensitive to the test compounds. It was found that ethanol and chloroform extracts of S. mukorossi and ethanol and benzene extracts of R. emodi inhibited Hpylori at very low concentrations, In the in vitro study, the isolates showed a considerable zone of inhibition at very low concentrations （10 μg/mL） for both the extracts. In the in vivo study, the H pylori infection was cleared with minimal doses of extracts of S. mukorossi （2.5 mg/mL） and R. emodi （3.0 mg/mL） given orally for seven days. CONCLUSION： We can conclude from this study that the extracts of S. mukorossi and R. emodi inhibited the growth of pylori in vitro and, in in vivo studies, the H pylori infection cleared within seven days at very low concentrations. We also found that H pylori did not acquire resistance against these herbal extracts even after 10 consecutive passages.
文摘Objective:To investigate the antihyperglycemic and antihyperlipidemie properties of hydroalcoholic extract of fruits of Sapindus mukorossi Caerlen and its beneficial effect on haeniatological parameters with histopathological analysis in streptpzotocin induced diabetic rats.Methods:Sapindus mukorossi fruits extract(250 and 500 mg/kg body weight) and standard drug glybenclamide(0.5 mg/kg body weight) were administered to diabetic rats.Effect of extract on hyperglycemia,hyperlipidemia and hematological parameters was studied in diabetic rats.Histopathological changes in diabetic rat pancreas were also observed after extract and glybenclamide treatment.Results:Daily oral administration of Sapindus mukorossi fruits extract(250 and 500 mg/kg body weight) and glybenclamide for 20 days showed beneficial effects on blood glucose level(P【0.01) and lipid level.The extract has a favorable effect on the histopathological changes of the pancreas in streptozotocin induced diabetes.Conclusions: These findings reveal that the hydroalcoholic extract of Sapindus mukorossi fruits extract possesses antihyperglycemic and antihyperlipidemie properties.In addition.the extract can prevent various complications of diabetes and improve some haeniatological parameters.
文摘Aim: To observe the alterations in the biochemical and biophysical changes in the sperm membrane during sperm maturation in male rats treated with the water extract of the fruit pericarp of S. mukorossi. Methods: Adult male Sprague-Dawley rats were gavaged the aqueous extract of the fruit pericarp of S. mukorossi at a dose of 50 mg/kg/d for 45 days. On day 46, the sperm parameters were observed in different sections of the epididymis and the sperm superoxide dismutase and the lipid peroxidation was determined and compared with the controls. The testis and epididymis were routinely prepared for histological examination under the light microscope. Results: No significant differences in the sperm number and morphology were observed between the control and treated groups. However, a significant inhibition (P<0.05-0.01) of sperm motility in the caput, corpus and cauda regions of the epididymis was seen in the treated group. No significant histopathological changes were found in the testis and epididymis. The important finding was that in the treated animals, the spermatozoa showed an abnormal distribution of the superoxide dismutase activity, being minimum in the caput and maximum in the corpus, which was just opposite to that of the controls. Conclusion: The study provides a unique observation where the plant extract alters the sperm membrane physiology without change the testicular and epididymal morphology.
基金funding provided by University of Malaya, Kuala Lumpur (No. PV102-2011A, UM-QUB6A-2011) for carrying out this research
文摘This study explores the possible application of a biodegradable plant based surfactant, obtained from Sapindus mukorossi, for washing low levels of arsenic （As） from an iron （Fe） rich soil. Natural association of As（Ⅴ） with Fe（Ⅲ） makes the process difficult. Soapnut solution was compared to anionic surfactant sodium dodecyl sulfate （SDS） in down-flow and a newly introduced suction mode for soil column washing. It was observed that soapnut attained up to 86% efficiency with respect to SDS in removing As. Full factorial design of experiment revealed a very good fit of data. The suction mode generated up to 83 kPa pressure inside column whilst down-flow mode generated a much higher pressure of 214 kPa, thus making the suction mode more efficient. Micellar solubilisation was found to be responsible for As desorption from the soil and it followed 1st order kinetics. Desorption rate coefficient of suction mode was found to be in the range of 0.005 to 0.01, much higher than down-flow mode values. Analysis of the PT-IR data suggested that the soapnut solution did not interact chemically with As, offering an option for reusing the surfactant. Soapnut can be considered as a soil washing agent for removing As even from soil with high Fe content.
文摘The study has been carried out to investigate the effects of single factors such as solvents extraction temperature, times, solid-liquid ration, and the time of extractions on the yields of saponins present in the pulp of Sapindus mukorossi Gaerth. On this basis, an L9 orthogonal design of experiment was adopted to determine the optimal conditions for the extraction of saponins. The factors that influence the extraction of saponins are put in the order of extraction times, extraction time, solid-liquid ration, and the best combination is that the powder of the pulp is extracted with EtOH (solid-to-solvent ratio = 1:8, w/v) for three times at 60°C for 3 hours. Under these conditions, about 1.63 g saponins will be extracted from 10 g raw material. The stability test showed that the Sapindus mukuross saponins can maintain surface activity at water temperature (25°C - 40°C), pH (6.3 - 7.7) and water hardness (50 - 250 mg.L-1). It is proved that Sapindus mukuross saponins are quality non-ionic active agent.
文摘Two new tirucallane-type triterpenoid saponins, Sapimukoside A (1) and Sapimukoside B (2), were isolated from the roots of Sapindus mukorossi Gaertn. On the basis of spectroscopic and chemical evidence, their structures were elucidated to be 3-O-alpha-L-rhamnopyranosyl-(1-->2)-[alpha-L-arabinopyranosyl-(1-->3)]-beta-D-glucopyranosyl-21, 23R-epoxyl tirucall-7, 24R-diene-3beta, 21-diol (1) and 3-O-alpha-L-rhamnopyranosyl-(1-->6)-beta-D-glucopyranosyl-21, 23R-epoxyl tirucall-7, 24R-diene-3beta, 21-diol (2).
基金supported by the Fundamental Research Funds for the Central Universities of China (2015ZCQLX-02 2017-LYSJWJ-1)。
文摘To explore differences in leaf morphology between Sapindus mukorossi and Sapindus delavayi,and how the environment might drive these differences,80 germplasm samples from the Sapindus germplasm nursery in Fujian Province were selected.The study revealed a wide variation and diversity in 16 germplasm traits,both within and between species grown under the same conditions.On average,the relative contribution of intraspecific variability to total variability was more important(83%) than the relative contribution of interspecific variability(17%).PERMANOVA analysis showed differences in leaflet thickness,length,perimeter,length to width ratio,and leaf hairs or trichome density.Correlation analyses between leaf morphological traits and environmental variables indicated that leaves tended to be larger,longer,and thicker in wetter,warmer,and low-altitude conditions.Our analysis of the relationship between climate and leaf morphology revealed that S.mukorossi had a greater sensitivity to climate variation,particularly in response to mean temperatures of the coldest and warmest seasons,which led to differences in leaf traits and the distribution of the two species.These findings contribute to the understanding of leaf morphology variations in S.mukorossi and S.delavayi,and provide a basis for the collection of Sapindus germplasm resources,their cultivation and use to help address climate change.
文摘[Objectives]To investigate the antimicrobial activity and mechanism for saponin from Sapindus mukorossi against Escherichia coli.[Methods]E.coli was used as the test bacteria,and the antimicrobial effect of Sapindus saponin was studied by the minimum inhibitory concentration method.The antimicrobial mechanism of Sapindus saponin was studied in terms of growth curves,membrane potential,activity of cells,cell surface morphology and cell internal structure.[Results]The results indicate that Sapindus saponin can inhibit the growth of E.coli,the minimum inhibitory concentration is 5 mg/mL and has a significant inhibitory effect on the growth of E.coli.After treated with Sapindus saponin,the membrane potential of E.coli increased by 16.7,17.3 and 19.1 times after exposition to 0.5,1,2 MIC Sapindus saponin respectively.PI staining results show that cell viability decreased and permeability of cell membranes increased.The results of SEM and TEM further corroborate the membrane damage,the cell was damaged after exposition to Sapindus saponin.[Conclusions]The antimicrobial mechanism of Sapindus saponin were to changed membrane potential of cell,and damage the cell membrane structures.
基金Supported by Forestry Science and Technology Planning Project of Hunan Province,China(XLK201511)
文摘In order to solve the problem of rapid propagation of Sapindus mukorossi Gaertn seedlings, research on the cutting propagation of S. mukorossi Gaertn was conducted. The results showed that the rooting rate of cuttings of the ten clones ranged from 30.9% to 83.0%. Among them, the rooting rate of cuttings of clones SM01 and SM06 was higher than that of other clones, and there were significant differences between different clones in the rooting rate of cuttings. The rooting rate of the semi-lignified shoots having two leaves could be improved significantly. The treatment of the cuttings by GGR6 solutions had significant effects on their rooting rate.
基金supported by from the Scientific and Technological Research Council of Turkey(TUBITAK),project No.215S627。
文摘Objective:Due to the many negative properties of sodium hypochlorite used in current root canal treatment,interest in biocompatible natural agents is increasing day by day.The aim of this study was to evaluate whether various extract solutions of Sapindus mukorossi have dissolution effects on human pulp tissues.Methods:Primarily powder extracts were obtained by extracting fruit shells of S.mukorossi in different solvents(ethanol,methanol,buthanol and distilled water).The test solutions were prepared and randomly separated into six groups with 10 samples in each group:ethanol extract,methanol extract,butanol extract,distilled water extract of S.mukorossi,sodium hypochlorite(Na OCl)and the control group.Among these,S.mukorossi extracts were separated into two subgroups,depending on their concentration level(50μg/m L and 100μg/m L).The pulp tissues of freshly extracted human molars were used for dissolution test.The weights of the pulpal tissues were measured and recorded for two times after the samples were placed in the solutions.Statistical analysis for all descriptive statistics was performed using SPSS 22(P<0.05).Results:Our results showed that maximum percent yield of preparation was obtained in methanol extract of S.mukorossi.Among all of the groups,the best dissolution capacity was seen in the Na OCl group(positive control group).Among S.mukorossi groups,the best tissue solvent solution was found in SMM group at 50μg/m L and SMB group at 100μg/m L.Conclusion:The different extracts of S.mukorossi had a capacity to dissolve pulp tissue but this capacity was less than Na OCl.Therefore,further studies will enable the creation of a commercial solution for clinical use by increasing the effectiveness of S.mukorossi while combining it with other endodontic irrigation solutions.
