AIM: To study the method of dissociation, culture and investigate its morphologic changes in vitro of interstitial cells of Cajal (ICC).METHODS: Enzymatic digestion and Ficoll density centrifugation were used to disso...AIM: To study the method of dissociation, culture and investigate its morphologic changes in vitro of interstitial cells of Cajal (ICC).METHODS: Enzymatic digestion and Ficoll density centrifugation were used to dissociate ICC from the ileal segment of mice. Factors including contamination, Ca2+, Mg2+ and collagenase, and stem cell factor, etc., were investigated.ACK2, the antibody of c-kit, was used to identify the cultured ICC. Both light microscope and fluorescence microscope were used to observe the changes of ICC in vitro.RESULTS: The method for dissociation and culture of ICC in vitro was successfully established. After 24 h, cultured ICC exhibited a few axis-cylinders, and longer axis-cylinders were observed to form synapse of each other after 3 d. More widespread connections formed within 7 d in vitro. The changes of its morphologic character were obvious within 7 d; however, there were no obvious morphologic changes after 30 d.CONCLUSION: Many factors can influence the dissociation and culture of ICC.展开更多
By tracking and monitoring the profile configuration, topography, and hydrodynamic factors of an artificial cobble beach in Tianquan Bay, Xiamen, China over three consecutive years after its completion, we analyzed th...By tracking and monitoring the profile configuration, topography, and hydrodynamic factors of an artificial cobble beach in Tianquan Bay, Xiamen, China over three consecutive years after its completion, we analyzed the evolution of its profile configura- tion and plane morphology, and its storm response characteristics. The evolution of the profile configuration of the artificial cobble beach in Tianquan Bay can be divided into four stages. The beach was unstable during the initial stage after the beach nourishment the profile configuration changed obviously, and an upper concave composite cobble beach formed gradually, which was character- ized by a steep upper part and a gentle lower part. In the second stage, the cobble beach approached dynamic equilibrium with minor changes in the profile configuration. At the third stage the beach was in a high-energy state under the influence of Typhoon Meranti, and the response of the artificial cobble beach differed significantly from that of the low-tide terrace sandy beach. Within a short time, there was net onshore transport of cobbles in the cross-shore direction. The beach face was eroded, the beach berm was accumulated, and the slope of the beach was steepened considerably. In the alongshore direction, there was notable transport of cobbles on the beach from east to west along the shore, and the total volume of the beach decreased by 4.5×103 m 3, which accounted for 50% of the total amount of beach volume lost within three years. The fourth stage was the restoration stage after the typhoon, characterized by a little gentler profile slope and the increase in width and the decrease in height of beach berm. Because of the action of waves and the wave-driven longshore current caused by the specific terrain and landform conditions along the coast (e.g., coastal headlands, near-shore artificial structures, and reefs), the coastline of the artificial cobble beach gradually evolved from being essentially parallel to the artificial coast upon completion to a slightly curved展开更多
It is easy to identify ancient fluvial morphologic types by the outcrop,log and core data.However,the horizontal distribution and geometry of the channels can only be identified and predicted by relying on the 3-D sei...It is easy to identify ancient fluvial morphologic types by the outcrop,log and core data.However,the horizontal distribution and geometry of the channels can only be identified and predicted by relying on the 3-D seismic data.The 3-D seismic horizon slices,especially,can play an important role in the sandstone prediction of meandering rivers,distributary channels and low-sinuosity channels.Every microfacies unit,including main channels,such as sinuous or branching channels,levee,crevasse channels,ligule crevasse splay and floodplain etc.can be identified.Braided channel sandstones are planar tabular lateral-connected sandbodies and the distribution of thick main channel belts can only be identified from 3-D seismic data.As the braided sandstones are ubiquitous,their occurrence and distribution do not need to be predicted.Generally,the coal velocity is so low that it can create a strong amplitude reflection in coal strata.It consequently conceals the amplitude respondence to anastomosing channel sandstone which could be identified from 3-D seismic inversion data sometimes.Case studies of mud-rich low-sinuosity rivers identified with 3-D seismic data indicate that the scales and width-to-thickness ratio of such sandbodies are small,laterally unconnected,and generally occurred on distant or further parts of an alluvial fan under dry climate conditions.Sometimes extraction of seismic attributes of every reflection event along horizons is expected to maximize expression of the spatial evolutions of ancient channels.展开更多
基金Supported by the National Natural Science Foundation of China, No. 30300156
文摘AIM: To study the method of dissociation, culture and investigate its morphologic changes in vitro of interstitial cells of Cajal (ICC).METHODS: Enzymatic digestion and Ficoll density centrifugation were used to dissociate ICC from the ileal segment of mice. Factors including contamination, Ca2+, Mg2+ and collagenase, and stem cell factor, etc., were investigated.ACK2, the antibody of c-kit, was used to identify the cultured ICC. Both light microscope and fluorescence microscope were used to observe the changes of ICC in vitro.RESULTS: The method for dissociation and culture of ICC in vitro was successfully established. After 24 h, cultured ICC exhibited a few axis-cylinders, and longer axis-cylinders were observed to form synapse of each other after 3 d. More widespread connections formed within 7 d in vitro. The changes of its morphologic character were obvious within 7 d; however, there were no obvious morphologic changes after 30 d.CONCLUSION: Many factors can influence the dissociation and culture of ICC.
基金supported by the Scientific Research Foundation of the Third Institute of Oceanography, State Oceanic Administration (Nos. 20170305, 2011010)the Public Science and Technology Research Funds Projects of Ocean (No. 201405037)
文摘By tracking and monitoring the profile configuration, topography, and hydrodynamic factors of an artificial cobble beach in Tianquan Bay, Xiamen, China over three consecutive years after its completion, we analyzed the evolution of its profile configura- tion and plane morphology, and its storm response characteristics. The evolution of the profile configuration of the artificial cobble beach in Tianquan Bay can be divided into four stages. The beach was unstable during the initial stage after the beach nourishment the profile configuration changed obviously, and an upper concave composite cobble beach formed gradually, which was character- ized by a steep upper part and a gentle lower part. In the second stage, the cobble beach approached dynamic equilibrium with minor changes in the profile configuration. At the third stage the beach was in a high-energy state under the influence of Typhoon Meranti, and the response of the artificial cobble beach differed significantly from that of the low-tide terrace sandy beach. Within a short time, there was net onshore transport of cobbles in the cross-shore direction. The beach face was eroded, the beach berm was accumulated, and the slope of the beach was steepened considerably. In the alongshore direction, there was notable transport of cobbles on the beach from east to west along the shore, and the total volume of the beach decreased by 4.5×103 m 3, which accounted for 50% of the total amount of beach volume lost within three years. The fourth stage was the restoration stage after the typhoon, characterized by a little gentler profile slope and the increase in width and the decrease in height of beach berm. Because of the action of waves and the wave-driven longshore current caused by the specific terrain and landform conditions along the coast (e.g., coastal headlands, near-shore artificial structures, and reefs), the coastline of the artificial cobble beach gradually evolved from being essentially parallel to the artificial coast upon completion to a slightly curved
基金supported by the National 973 Project (No.2003CB214602)
文摘It is easy to identify ancient fluvial morphologic types by the outcrop,log and core data.However,the horizontal distribution and geometry of the channels can only be identified and predicted by relying on the 3-D seismic data.The 3-D seismic horizon slices,especially,can play an important role in the sandstone prediction of meandering rivers,distributary channels and low-sinuosity channels.Every microfacies unit,including main channels,such as sinuous or branching channels,levee,crevasse channels,ligule crevasse splay and floodplain etc.can be identified.Braided channel sandstones are planar tabular lateral-connected sandbodies and the distribution of thick main channel belts can only be identified from 3-D seismic data.As the braided sandstones are ubiquitous,their occurrence and distribution do not need to be predicted.Generally,the coal velocity is so low that it can create a strong amplitude reflection in coal strata.It consequently conceals the amplitude respondence to anastomosing channel sandstone which could be identified from 3-D seismic inversion data sometimes.Case studies of mud-rich low-sinuosity rivers identified with 3-D seismic data indicate that the scales and width-to-thickness ratio of such sandbodies are small,laterally unconnected,and generally occurred on distant or further parts of an alluvial fan under dry climate conditions.Sometimes extraction of seismic attributes of every reflection event along horizons is expected to maximize expression of the spatial evolutions of ancient channels.
