中医药治疗肿瘤相关性抑郁(cancer related depression,CRD)的作用机制主要包括:调节下丘脑-垂体-肾上腺轴功能,提高单胺类神经递质的水平,调节细胞因子水平(包括调节血管生成的相关因子水平、下调炎性细胞因子),调节免疫系统等。目前,...中医药治疗肿瘤相关性抑郁(cancer related depression,CRD)的作用机制主要包括:调节下丘脑-垂体-肾上腺轴功能,提高单胺类神经递质的水平,调节细胞因子水平(包括调节血管生成的相关因子水平、下调炎性细胞因子),调节免疫系统等。目前,研究存在的问题是:(1)中医药治疗CRD的研究主要集中于中药复方,对单味中药及有效成分研究较少;(2)纳入文献样本量少,缺乏多中心、大样本、双盲的随机对照研究,并且大多数研究为中西医结合治疗,缺乏中药单药治疗CRD的相关研究;(3)作用机制研究中同一中药复方的基础研究与临床研究不能紧密联系;(4)多数研究集中于中医药治疗CRD的疗效,缺乏毒理作用的研究,无法进一步阐明其安全性;(5)中医药与现代科学技术的结合力度小,缺乏中药复方有效成分其靶点的研究;(6)中医药研究集中于治疗CRD,对如何有效预防CRD及何时进行干预仍有待进一步研究。今后,应将基础研究与大样本、多中心、双盲的临床研究相结合,进一步证实其作用机制;与此同时,将中医药与现代科学技术相结合,实现精准化防治结合。展开更多
Abstract： In the presem study, we simultaneously quantified the levels of monoamine neurotransmitters （MANTs） and their metabolites （levodopa, norepinephrine, epinephrine, dopamine, 5-HT, 3,4-dihydroxyphenylacetic...Abstract： In the presem study, we simultaneously quantified the levels of monoamine neurotransmitters （MANTs） and their metabolites （levodopa, norepinephrine, epinephrine, dopamine, 5-HT, 3,4-dihydroxyphenylacetic acid, homovanillic acid and 5-hydroxyindole-3-acetic acid） in different brain subregions of rats using a newly developed simple, sensitive and selective high-performance liquid chromatography with fluorescence detection （HPLC-FLD） method. In this new HPLC-FLD method, analytes were directly extracted and separated without deriveatization step within 20 min. The FLD wavelength was set at 280 nm and 330 nm for excitation and emission, respectively. The analytes were separated on an Agilent Eclipse Plus Cls column （4.6 mm×150 mm, 5.0 μm） equipped with an Agilent XDB-C18 security guard column （4.6 mm×12.5 mm, 5.0 lam）, and the column temperature was maintained at 35 ℃. The mobile phase for elution was isocratic. The mobile phase consisted of citric acid buffer （50 mmol/L citric acid, 50 mmol/L sodium acetate, 0.5 mmol/L octane sulfonic acid sodium salt, 0.5 mmol/L Na2EDTA and 5 mmol/L triethylamine, pH 3.8） and methanol （90：10, v/v） at a flow rate of 1.0 mL/min. The detection limit （DL） was 0.9-23 nM for all the MANTs and their metabolites with a sample volume of 50 μL. The method was shown to be highly reproducible in terms of peak area （intraday, 0.08%-1.85% RSD, n = 5）. The simultaneous measurement of these MANTs and their metabolites improved our understanding of the neurochemistry in the central nervous system （CNS） in relation to different addictive drugs （methamphetamine, heroin and their mixture） in drug-addicted rat models.展开更多
文摘中医药治疗肿瘤相关性抑郁(cancer related depression,CRD)的作用机制主要包括:调节下丘脑-垂体-肾上腺轴功能,提高单胺类神经递质的水平,调节细胞因子水平(包括调节血管生成的相关因子水平、下调炎性细胞因子),调节免疫系统等。目前,研究存在的问题是:(1)中医药治疗CRD的研究主要集中于中药复方,对单味中药及有效成分研究较少;(2)纳入文献样本量少,缺乏多中心、大样本、双盲的随机对照研究,并且大多数研究为中西医结合治疗,缺乏中药单药治疗CRD的相关研究;(3)作用机制研究中同一中药复方的基础研究与临床研究不能紧密联系;(4)多数研究集中于中医药治疗CRD的疗效,缺乏毒理作用的研究,无法进一步阐明其安全性;(5)中医药与现代科学技术的结合力度小,缺乏中药复方有效成分其靶点的研究;(6)中医药研究集中于治疗CRD,对如何有效预防CRD及何时进行干预仍有待进一步研究。今后,应将基础研究与大样本、多中心、双盲的临床研究相结合,进一步证实其作用机制;与此同时,将中医药与现代科学技术相结合,实现精准化防治结合。
基金National Natural Science Foundation(Grant No.813 73372)the Open Foundation of State Key Laboratory of Natural and Biomimetic Drugs(Grant No.SKL2012004)Specialized Research Fund for the Doctoral Program of Higher Education of China(Grant No.20110001110021 and 20130001110059)
文摘Abstract： In the presem study, we simultaneously quantified the levels of monoamine neurotransmitters （MANTs） and their metabolites （levodopa, norepinephrine, epinephrine, dopamine, 5-HT, 3,4-dihydroxyphenylacetic acid, homovanillic acid and 5-hydroxyindole-3-acetic acid） in different brain subregions of rats using a newly developed simple, sensitive and selective high-performance liquid chromatography with fluorescence detection （HPLC-FLD） method. In this new HPLC-FLD method, analytes were directly extracted and separated without deriveatization step within 20 min. The FLD wavelength was set at 280 nm and 330 nm for excitation and emission, respectively. The analytes were separated on an Agilent Eclipse Plus Cls column （4.6 mm×150 mm, 5.0 μm） equipped with an Agilent XDB-C18 security guard column （4.6 mm×12.5 mm, 5.0 lam）, and the column temperature was maintained at 35 ℃. The mobile phase for elution was isocratic. The mobile phase consisted of citric acid buffer （50 mmol/L citric acid, 50 mmol/L sodium acetate, 0.5 mmol/L octane sulfonic acid sodium salt, 0.5 mmol/L Na2EDTA and 5 mmol/L triethylamine, pH 3.8） and methanol （90：10, v/v） at a flow rate of 1.0 mL/min. The detection limit （DL） was 0.9-23 nM for all the MANTs and their metabolites with a sample volume of 50 μL. The method was shown to be highly reproducible in terms of peak area （intraday, 0.08%-1.85% RSD, n = 5）. The simultaneous measurement of these MANTs and their metabolites improved our understanding of the neurochemistry in the central nervous system （CNS） in relation to different addictive drugs （methamphetamine, heroin and their mixture） in drug-addicted rat models.
