AIM： To compare the anti-inflammatory properties of butyrate with two other SCFAs, namely acetate and propionate, which have less well-documented effects on inflammation. METHODS： The effect of SCFAs on cytokine rel...AIM： To compare the anti-inflammatory properties of butyrate with two other SCFAs, namely acetate and propionate, which have less well-documented effects on inflammation. METHODS： The effect of SCFAs on cytokine release from human neutrophils was studied with EHSA. SCFA- dependent modulation of NF-κB reporter activity was assessed in the human colon adenocarcinoma cell line, Colo320DM. Finally, the effect of SCFAs on gene expression and cytokine release, measured with RT-PCR and ELISA, respectively, was studied in mouse colon organ cultures established from colitic mice. RESULTS： Acetate, propionate and butyrate at 30 mmol/L decreased LPS-stimulated TNFα release from neutrophils, without affecting IL-8 protein release. All SCFAs dose dependently inhibited NF-κB reporter activity in Colo320DM cells. Propionate dose-dependently suppressed IL-6 mRNA and protein release from colon organ cultures and comparative studies revealed that propionate and butyrate at 30 mmol/L caused a strong inhibition of immune-related gene expression, whereas acetate was less effective. A similar inhibition was achieved with the proteasome inhibitor MG-132, but not the p38 MAPK inhibitor SB203580. All SCFAs decreased IL-6 protein release from organ cultures. CONCLUSION： In the present study propionate and butyrate were equipotent, whereas acetate was less effective, at suppressing NF-κB reporter activity, immune-related gene expression and cytokine release in vitro. Our findings suggest that propionate and acetate, in addition to butyrate, could be useful in the treatment of inflammatory disorders, including IBD.展开更多
[目的]观察疏肝利胆汤治疗慢性胆囊炎疗效。[方法]使用随机平行对照方法,将120例住院及门诊患者按抛硬币法简单随机分为两组。对照组60例10m L 50%硫磺镁,1次/d,口服;头抱挫林钠+250m L 4%生理盐水,1次/d,静滴;甲硝唑,200m L/次,1次/d,...[目的]观察疏肝利胆汤治疗慢性胆囊炎疗效。[方法]使用随机平行对照方法,将120例住院及门诊患者按抛硬币法简单随机分为两组。对照组60例10m L 50%硫磺镁,1次/d,口服;头抱挫林钠+250m L 4%生理盐水,1次/d,静滴;甲硝唑,200m L/次,1次/d,静滴。治疗组60例疏肝利胆汤(柴胡、枳壳、赤芍、木香、黄芪、鸡内金、郁金、川厚朴、山楂各10g,生甘草8g,黄连6g,熟大黄8g;胁痛严重加元胡10g;蛔虫加使君子10g,川谏子、槟榔各6g;胁痛、肠鸣腹泻减枳实,加白术、获菩各10g;恶心、呕吐加竹茹10g;苔厚腻夹湿加苍术6g、陈皮、获菩各10g),1剂/d,水煎200m L,早晚温服。连续治疗15d为1疗程。观测临床症状、不良反应。连续治疗3疗程,判定疗效。[结果]治疗组痊愈50例,有效9例,无效1例,总有效率98.33%。对照组痊愈40例,有效10例,无效10例,总有效率83.33%。治疗组疗效优于对照组(P<0.01)。[结论]疏肝利胆汤治疗慢性胆囊炎疗效满意,无严重不良反应,值得推广。展开更多
文摘AIM： To compare the anti-inflammatory properties of butyrate with two other SCFAs, namely acetate and propionate, which have less well-documented effects on inflammation. METHODS： The effect of SCFAs on cytokine release from human neutrophils was studied with EHSA. SCFA- dependent modulation of NF-κB reporter activity was assessed in the human colon adenocarcinoma cell line, Colo320DM. Finally, the effect of SCFAs on gene expression and cytokine release, measured with RT-PCR and ELISA, respectively, was studied in mouse colon organ cultures established from colitic mice. RESULTS： Acetate, propionate and butyrate at 30 mmol/L decreased LPS-stimulated TNFα release from neutrophils, without affecting IL-8 protein release. All SCFAs dose dependently inhibited NF-κB reporter activity in Colo320DM cells. Propionate dose-dependently suppressed IL-6 mRNA and protein release from colon organ cultures and comparative studies revealed that propionate and butyrate at 30 mmol/L caused a strong inhibition of immune-related gene expression, whereas acetate was less effective. A similar inhibition was achieved with the proteasome inhibitor MG-132, but not the p38 MAPK inhibitor SB203580. All SCFAs decreased IL-6 protein release from organ cultures. CONCLUSION： In the present study propionate and butyrate were equipotent, whereas acetate was less effective, at suppressing NF-κB reporter activity, immune-related gene expression and cytokine release in vitro. Our findings suggest that propionate and acetate, in addition to butyrate, could be useful in the treatment of inflammatory disorders, including IBD.
文摘[目的]观察疏肝利胆汤治疗慢性胆囊炎疗效。[方法]使用随机平行对照方法,将120例住院及门诊患者按抛硬币法简单随机分为两组。对照组60例10m L 50%硫磺镁,1次/d,口服;头抱挫林钠+250m L 4%生理盐水,1次/d,静滴;甲硝唑,200m L/次,1次/d,静滴。治疗组60例疏肝利胆汤(柴胡、枳壳、赤芍、木香、黄芪、鸡内金、郁金、川厚朴、山楂各10g,生甘草8g,黄连6g,熟大黄8g;胁痛严重加元胡10g;蛔虫加使君子10g,川谏子、槟榔各6g;胁痛、肠鸣腹泻减枳实,加白术、获菩各10g;恶心、呕吐加竹茹10g;苔厚腻夹湿加苍术6g、陈皮、获菩各10g),1剂/d,水煎200m L,早晚温服。连续治疗15d为1疗程。观测临床症状、不良反应。连续治疗3疗程,判定疗效。[结果]治疗组痊愈50例,有效9例,无效1例,总有效率98.33%。对照组痊愈40例,有效10例,无效10例,总有效率83.33%。治疗组疗效优于对照组(P<0.01)。[结论]疏肝利胆汤治疗慢性胆囊炎疗效满意,无严重不良反应,值得推广。
