AIM: To analyze the association of HCV-RNA with peripheral blood mononuclear cells (PBMC) and to answer the question whether HCV-RNA positivity in PBMC is due to viral replication. METHODS: HCV-RNA was monitored in se...AIM: To analyze the association of HCV-RNA with peripheral blood mononuclear cells (PBMC) and to answer the question whether HCV-RNA positivity in PBMC is due to viral replication. METHODS: HCV-RNA was monitored in serum and PBMC preparations from 15 patients with chronic HCV infection before, during and after an IFN-alpha therapy using a nested RT/PCR technique. In a second approach, PBMC from healthy donors were incubated in HCV positive plasma. RESULTS: In the IFN-alpha responding patients,HCV-RNA disappeared first from total RNA preparations of PBMC and then from serum. In contrast, in relapsing patients, HCV-RNA reappeared first in serum and then in PBMC. A quantitative analysis of the HCV-RNA concentration in serum was performed before and after transition from detectable to non detectable HCV-RNA in PBMC-RNA and vice versa. When HCV-RNA was detectable in PBMC preparations, the HCV concentration in serum was significantly higher than the serum HCV-RNA concentration when HCV-RNA in PBMC was not detectable. Furthermore, at no time during the observation period was HCV specific RNA observed in PBMC, if HCV-RNA in serum was under the detection limit. Incubation of PBMC from healthy donors with several dilutions of HCV positive plasma for two hours showed a concentration dependent PCR positivity for HCV-RNA in reisolated PBMC. CONCLUSION: The detectability of HCV-RNA in total RNA from PBMC seems to depend on the HCV concentration in serum. Contamination or passive adsorption by circulating virus could be the reason for detection of HCV-RNA in PBMC preparations of chronically infected patients.展开更多
AIM:The significance of hepatitis C virus (HCV) serum titers has been examined in several clinical situations. There is much evidence that patients with a lower viral load have better response rates to anti-viral ther...AIM:The significance of hepatitis C virus (HCV) serum titers has been examined in several clinical situations. There is much evidence that patients with a lower viral load have better response rates to anti-viral therapy compared to those with higher levels.Moreover,a direct association has been observed between serum titers of HCV and transmission rates of the virus.The aim of the present study was to determine if there was any correlation between HCV viral load and the severity of liver disease. METHODS:Fifty patients with HCV infection were included in the study.These comprised of 34 subjects with a history of alcohol use and 16 non-alcoholics.Quantitative serum HCV RNA assay was carried out using the branched DNA (bDNA) technique.Linear regression analysis was performed between serum viral titers and liver tests.In addition,for the purpose of comparison,the subjects were divided into two groups:those with low viral liters (≤50 genome mEq/mL) and high titers (>50 mEq/mL). RESULTS:All subjects were men,with a mean±SD age of 47±7.8 years.The mean HCV RNA level in the blood was 76.3×10~5±109.1 genome equivalents/mL.There was no correlation between HCV RNA levels and age of the patients (r=0.181),and the history or amount (g/d) of alcohol consumption (r=0.07).Furthermore,no correlation was observed between serum HCV RNA levels and the severity of liver disease as judged by the values of serum albumin (r=0.175),bilirubin (r=0.217),ALT (r=0.06) and AST (r=0.004) levels.Similarly,no significant difference was observed between patients with low viral titers and high liters with respect to any of the parameters. CONCLUSION:Our results indicate that the severity of liver disease is independent of serum levels of hepatitis C virus.These findings are important since they have a direct impact on the current debate regarding the role of direct cytopathic effect of hepatitis C virus versus immune-mediated injury in the pathogenesis of HCV-related liver damage.展开更多
The value of friction coefficient between the main cable and saddle, relates to not only the anti-slippage stability of three-tower suspension bridge, but also the reasonable stiffness of the middle tower and the magn...The value of friction coefficient between the main cable and saddle, relates to not only the anti-slippage stability of three-tower suspension bridge, but also the reasonable stiffness of the middle tower and the magnitude of rigidity of the whole bridge. First, the paper does some comparative studies about the relevant provisions of international norms, and then, summarizes the relevant load test results both at home and abroad. Finally, the paper draws the appropriate anti-slippage safety factor for the most unfavorable load in accordance with international load standards, and discusses the rationality and feasibility of the friction coefficient of 0.2 between main cable and saddle.展开更多
Objective: To investigate the efficacy of Zhongyan-2 Recipe (ZY-2), a recipe consisting of Chinese herbal medicine. Methods: Twenty-nine HIV-infected patients were treated with ZY-2 for 3 months as one treatment cours...Objective: To investigate the efficacy of Zhongyan-2 Recipe (ZY-2), a recipe consisting of Chinese herbal medicine. Methods: Twenty-nine HIV-infected patients were treated with ZY-2 for 3 months as one treatment course; and viral load, immune function and symptoms-signs were determined before and after treatment. Results: After treatment, the absolute values of CD4 were elevated in 8 cases, and lowered in 14 cases, 7 cases were not changed; the ratio of CD4/CD8 were elevated in 2 cases, lowered in 1 case, 16 of them were not changed obviously. The CD4 and CD4/CD8 ratio were elevated simultaneously in 2 cases. Neopterin lowered in 4 cases, elevated in 11 cases, and not changed in 1 case. Viral load (VL) was lowered in 6 cases, elevated in 8 cases and not changed in 2 cases. The total effective rate was 42.28% on the basis of comprehensive assessment of VL, immune function, body weight and symptoms-signs. Conclusion: ZY-2 is an effective Chinese recipe in treating HIV-infected patients.展开更多
The sediment sequence analysis of Mann-Kendall method based on major rivers of 10 hydrological station in the middle reaches of the Yellow River*[1]*t,.The results show that:The main rivers in the middle reaches of th...The sediment sequence analysis of Mann-Kendall method based on major rivers of 10 hydrological station in the middle reaches of the Yellow River*[1]*t,.The results show that:The main rivers in the middle reaches of the Yellow River hydrologic station sediment overall showed a trend of decreased significantly.Sediment discharge of all stations except Gao Jiachuan station have reached the maximum in 1956-1969s[2_3].Among various hydrologic station sediment discharge of inter-generational are generally shows the tendency of reducing year by year.Calculate the sediment transport of major river basin of Yellow River,which average is 0.63.展开更多
基金Supported by a grant of DFG (SFB 402 Teilprojekt C1 (Mihm))by a grant of Hoffmann La Roche (Grenzach-Wyhden, Germany)Part of the data has been presented as poster at the 1999 EASL-meeting in Neaples
文摘AIM: To analyze the association of HCV-RNA with peripheral blood mononuclear cells (PBMC) and to answer the question whether HCV-RNA positivity in PBMC is due to viral replication. METHODS: HCV-RNA was monitored in serum and PBMC preparations from 15 patients with chronic HCV infection before, during and after an IFN-alpha therapy using a nested RT/PCR technique. In a second approach, PBMC from healthy donors were incubated in HCV positive plasma. RESULTS: In the IFN-alpha responding patients,HCV-RNA disappeared first from total RNA preparations of PBMC and then from serum. In contrast, in relapsing patients, HCV-RNA reappeared first in serum and then in PBMC. A quantitative analysis of the HCV-RNA concentration in serum was performed before and after transition from detectable to non detectable HCV-RNA in PBMC-RNA and vice versa. When HCV-RNA was detectable in PBMC preparations, the HCV concentration in serum was significantly higher than the serum HCV-RNA concentration when HCV-RNA in PBMC was not detectable. Furthermore, at no time during the observation period was HCV specific RNA observed in PBMC, if HCV-RNA in serum was under the detection limit. Incubation of PBMC from healthy donors with several dilutions of HCV positive plasma for two hours showed a concentration dependent PCR positivity for HCV-RNA in reisolated PBMC. CONCLUSION: The detectability of HCV-RNA in total RNA from PBMC seems to depend on the HCV concentration in serum. Contamination or passive adsorption by circulating virus could be the reason for detection of HCV-RNA in PBMC preparations of chronically infected patients.
文摘AIM:The significance of hepatitis C virus (HCV) serum titers has been examined in several clinical situations. There is much evidence that patients with a lower viral load have better response rates to anti-viral therapy compared to those with higher levels.Moreover,a direct association has been observed between serum titers of HCV and transmission rates of the virus.The aim of the present study was to determine if there was any correlation between HCV viral load and the severity of liver disease. METHODS:Fifty patients with HCV infection were included in the study.These comprised of 34 subjects with a history of alcohol use and 16 non-alcoholics.Quantitative serum HCV RNA assay was carried out using the branched DNA (bDNA) technique.Linear regression analysis was performed between serum viral titers and liver tests.In addition,for the purpose of comparison,the subjects were divided into two groups:those with low viral liters (≤50 genome mEq/mL) and high titers (>50 mEq/mL). RESULTS:All subjects were men,with a mean±SD age of 47±7.8 years.The mean HCV RNA level in the blood was 76.3×10~5±109.1 genome equivalents/mL.There was no correlation between HCV RNA levels and age of the patients (r=0.181),and the history or amount (g/d) of alcohol consumption (r=0.07).Furthermore,no correlation was observed between serum HCV RNA levels and the severity of liver disease as judged by the values of serum albumin (r=0.175),bilirubin (r=0.217),ALT (r=0.06) and AST (r=0.004) levels.Similarly,no significant difference was observed between patients with low viral titers and high liters with respect to any of the parameters. CONCLUSION:Our results indicate that the severity of liver disease is independent of serum levels of hepatitis C virus.These findings are important since they have a direct impact on the current debate regarding the role of direct cytopathic effect of hepatitis C virus versus immune-mediated injury in the pathogenesis of HCV-related liver damage.
基金National Science and Technology Support Program of China(No.2009BAG15B01)
文摘The value of friction coefficient between the main cable and saddle, relates to not only the anti-slippage stability of three-tower suspension bridge, but also the reasonable stiffness of the middle tower and the magnitude of rigidity of the whole bridge. First, the paper does some comparative studies about the relevant provisions of international norms, and then, summarizes the relevant load test results both at home and abroad. Finally, the paper draws the appropriate anti-slippage safety factor for the most unfavorable load in accordance with international load standards, and discusses the rationality and feasibility of the friction coefficient of 0.2 between main cable and saddle.
文摘Objective: To investigate the efficacy of Zhongyan-2 Recipe (ZY-2), a recipe consisting of Chinese herbal medicine. Methods: Twenty-nine HIV-infected patients were treated with ZY-2 for 3 months as one treatment course; and viral load, immune function and symptoms-signs were determined before and after treatment. Results: After treatment, the absolute values of CD4 were elevated in 8 cases, and lowered in 14 cases, 7 cases were not changed; the ratio of CD4/CD8 were elevated in 2 cases, lowered in 1 case, 16 of them were not changed obviously. The CD4 and CD4/CD8 ratio were elevated simultaneously in 2 cases. Neopterin lowered in 4 cases, elevated in 11 cases, and not changed in 1 case. Viral load (VL) was lowered in 6 cases, elevated in 8 cases and not changed in 2 cases. The total effective rate was 42.28% on the basis of comprehensive assessment of VL, immune function, body weight and symptoms-signs. Conclusion: ZY-2 is an effective Chinese recipe in treating HIV-infected patients.
文摘The sediment sequence analysis of Mann-Kendall method based on major rivers of 10 hydrological station in the middle reaches of the Yellow River*[1]*t,.The results show that:The main rivers in the middle reaches of the Yellow River hydrologic station sediment overall showed a trend of decreased significantly.Sediment discharge of all stations except Gao Jiachuan station have reached the maximum in 1956-1969s[2_3].Among various hydrologic station sediment discharge of inter-generational are generally shows the tendency of reducing year by year.Calculate the sediment transport of major river basin of Yellow River,which average is 0.63.
