The study of microbial communities in the produced water of coalbed methane(CBM)wells is an important aspect of microbial-enhanced methane production.Water produced from 15 CBM wells in four synclines in eastern Yunna...The study of microbial communities in the produced water of coalbed methane(CBM)wells is an important aspect of microbial-enhanced methane production.Water produced from 15 CBM wells in four synclines in eastern Yunnan and western Guizhou was collected.Through the use of 16S ribosomal RNA(16S rRNA)amplicon sequencing and realtime fluorescence quantitative polymerase chain reaction(PCR),the characteristics of bacterial and archaeal communities before and after enrichment culture were studied.The methanogenic pathways of secondary biogas were discussed,and potential microbial-enhanced methane production was preliminarily evaluated.The results showed that the bacterial DNA content in uncultured produced water was low,so it is difficult to detect.After enrichment,the dominant bacteria phyla were Proteobacteria,Bacteroidetes,and Firmicutes.A total of seven phyla were detected in the uncultured produced water,and the dominant archaeal phylum was Euyarchaeota.Methanogens were the main component of archaea.The dominant archaeal genera were Methanobacterium,Methanoculleus and Methanobrevibacter.The community structure of the archaea changed noticeably after four days of enrichment culture.The relative abundance of Euryarchaeota increased to 99%in most samples after enrichment culture.It was found that there was a transition from Methanoregula to Methanobacterium within genera.The relative abundance of Methanobacterium increased,which can produce hydrogenotrophic methane.Combined with the isotopic composition of the produced water and gas,it is considered that the CBM in the Tucheng and Enhong synlines consists of a mixture of thermogenic gas and biogas.The proportion of secondary biogas in the Tucheng and Enhong synlines are estimated to range from 10.89% to 49.62%.There are mainly hydrogentrophic methanogens in the study area,and CO_(2) reduction is the main way of microbial gas production.After enrichment culture of produced water in the study area,the hydrogenotrophic methanogens were enriched.These two areas have stro展开更多
以16S r DNA为分子标记,采用高通量测序技术分析吉林油田FY区块D1、D2和D3三口采油井中的微生物群落构成.对3个DNA样本中细菌16S r DNA的PCR扩增产物进行高通量测序,得到123 360条优化序列,测序深度指数超过99.9%.根据序列相似性进行聚...以16S r DNA为分子标记,采用高通量测序技术分析吉林油田FY区块D1、D2和D3三口采油井中的微生物群落构成.对3个DNA样本中细菌16S r DNA的PCR扩增产物进行高通量测序,得到123 360条优化序列,测序深度指数超过99.9%.根据序列相似性进行聚类分析,得到139个OTU.基于OTU的物种分类分析,发现3个样本中的细菌种类覆盖91个属29个纲20个门,其中包括多种采油有益菌.分别对各个样本的菌种组成和相对丰度进行分析,发现不同采油井的主要菌种组成和优势类群呈现出差异性.D1中以γ-proteobacteria(52%)和ε-proteobacteria(39%)为主,优势属为Pseudomonas(51%)和Arcobacter(38%);D2中以ε-proteobacteria(88%)为主,优势属为Arcobacter(88%);D3中以α-proteobacteria(55%)、ε-proteobacteria(20%)和β-proteobacter ia(19%)为主,优势属为Rhizobium(36%)和Arcobacter(20%).本研究结果可为油藏微生物资源的开发利用和微生物采油技术的开展提供精确全面的背景信息支持.(图6表1参27)展开更多
基金Financial support for this work was provided by the “14th Five-Year Plan” forward-looking basic major scientific and technological project of China National Petroleum Corporation’s (No. 2021DJ2303)the National Natural Science Foundation of China (Grant Nos. 42272195 and 42130802)Guizhou Provincial Science and Technology Program: Qiankehe Strategic Mineral Search (No. [2022] ZD001-01).
文摘The study of microbial communities in the produced water of coalbed methane(CBM)wells is an important aspect of microbial-enhanced methane production.Water produced from 15 CBM wells in four synclines in eastern Yunnan and western Guizhou was collected.Through the use of 16S ribosomal RNA(16S rRNA)amplicon sequencing and realtime fluorescence quantitative polymerase chain reaction(PCR),the characteristics of bacterial and archaeal communities before and after enrichment culture were studied.The methanogenic pathways of secondary biogas were discussed,and potential microbial-enhanced methane production was preliminarily evaluated.The results showed that the bacterial DNA content in uncultured produced water was low,so it is difficult to detect.After enrichment,the dominant bacteria phyla were Proteobacteria,Bacteroidetes,and Firmicutes.A total of seven phyla were detected in the uncultured produced water,and the dominant archaeal phylum was Euyarchaeota.Methanogens were the main component of archaea.The dominant archaeal genera were Methanobacterium,Methanoculleus and Methanobrevibacter.The community structure of the archaea changed noticeably after four days of enrichment culture.The relative abundance of Euryarchaeota increased to 99%in most samples after enrichment culture.It was found that there was a transition from Methanoregula to Methanobacterium within genera.The relative abundance of Methanobacterium increased,which can produce hydrogenotrophic methane.Combined with the isotopic composition of the produced water and gas,it is considered that the CBM in the Tucheng and Enhong synlines consists of a mixture of thermogenic gas and biogas.The proportion of secondary biogas in the Tucheng and Enhong synlines are estimated to range from 10.89% to 49.62%.There are mainly hydrogentrophic methanogens in the study area,and CO_(2) reduction is the main way of microbial gas production.After enrichment culture of produced water in the study area,the hydrogenotrophic methanogens were enriched.These two areas have stro
文摘以16S r DNA为分子标记,采用高通量测序技术分析吉林油田FY区块D1、D2和D3三口采油井中的微生物群落构成.对3个DNA样本中细菌16S r DNA的PCR扩增产物进行高通量测序,得到123 360条优化序列,测序深度指数超过99.9%.根据序列相似性进行聚类分析,得到139个OTU.基于OTU的物种分类分析,发现3个样本中的细菌种类覆盖91个属29个纲20个门,其中包括多种采油有益菌.分别对各个样本的菌种组成和相对丰度进行分析,发现不同采油井的主要菌种组成和优势类群呈现出差异性.D1中以γ-proteobacteria(52%)和ε-proteobacteria(39%)为主,优势属为Pseudomonas(51%)和Arcobacter(38%);D2中以ε-proteobacteria(88%)为主,优势属为Arcobacter(88%);D3中以α-proteobacteria(55%)、ε-proteobacteria(20%)和β-proteobacter ia(19%)为主,优势属为Rhizobium(36%)和Arcobacter(20%).本研究结果可为油藏微生物资源的开发利用和微生物采油技术的开展提供精确全面的背景信息支持.(图6表1参27)
