Microbial fermentation has contributed to 80%of global amino acid production.The key to microbial fermentation is to obtain fermentation strains with high performance to produce target amino acids with a high yield.Th...Microbial fermentation has contributed to 80%of global amino acid production.The key to microbial fermentation is to obtain fermentation strains with high performance to produce target amino acids with a high yield.These strains are primarily derived from screening enormous mutant libraries.Therefore,a high-throughput,rapid,accurate,and universal screening strategy for amino acid overproducers has become a guarantee for ob-taining optional amino acid overproducers.In recent years,the rapid development of various novel screening strategies has been witnessed.However,proper analysis and discussion of these innovative technologies are lacking.Here we systematically reviewed recent advances in screening strategies:the auxotrophic-based strategy,the biosensor-based strategy,and the latest translation-based screening strategy.The design principle,application scope,working efficiency,screening accuracy,and universality of these strategies were discussed in detail.The potential for screening nonstandard amino acid overproducers was also analyzed.Guidance for the improvement of future screening strategies is provided in this review,which could expedite the reconstruction of amino acid overproducers and help promote the fermentation industry to reduce cost,increase yield,and improve quality.展开更多
基金supported by the National Key R&D Program of China(2019YFA0906500)the National Natural Science Foundation of China(32000059)+1 种基金the Innovation Team Project of Colleges and Universities in Jinan(2019GXRC033)the Fundamental Research Funds for the Central Universities.
文摘Microbial fermentation has contributed to 80%of global amino acid production.The key to microbial fermentation is to obtain fermentation strains with high performance to produce target amino acids with a high yield.These strains are primarily derived from screening enormous mutant libraries.Therefore,a high-throughput,rapid,accurate,and universal screening strategy for amino acid overproducers has become a guarantee for ob-taining optional amino acid overproducers.In recent years,the rapid development of various novel screening strategies has been witnessed.However,proper analysis and discussion of these innovative technologies are lacking.Here we systematically reviewed recent advances in screening strategies:the auxotrophic-based strategy,the biosensor-based strategy,and the latest translation-based screening strategy.The design principle,application scope,working efficiency,screening accuracy,and universality of these strategies were discussed in detail.The potential for screening nonstandard amino acid overproducers was also analyzed.Guidance for the improvement of future screening strategies is provided in this review,which could expedite the reconstruction of amino acid overproducers and help promote the fermentation industry to reduce cost,increase yield,and improve quality.
