In natural environments,plants are exposed to diverse microbiota that they interact with in complex ways.While plant-pathogen interactions have been intensely studied to understand defense mechanisms in plants,many mi...In natural environments,plants are exposed to diverse microbiota that they interact with in complex ways.While plant-pathogen interactions have been intensely studied to understand defense mechanisms in plants,many microbes and microbial communities can have substantial beneficial effects on their plant host.Such beneficial effects include improved acquisition of nutrients,accelerated growth,resilience against pathogens,and improved resistance against abiotic stress conditions such as heat,drought,and salinity.However,the beneficial effects of bacterial strains or consortia on their host are often cultivar and species specific,posing an obstacle to their general application.Remarkably,many of the signals that trigger plant immune responses are molecularly highly similar and often identical in pathogenic and beneficial microbes.Thus,it is unclear what determines the outcome of a particular microbe-host interaction and which factors enable plants to distinguish beneficials from pathogens.To unravel the complex network of genetic,microbial,and metabolic interactions,including the signaling events mediating microbe-host interactions,comprehensive quantitative systems biology approaches will be needed.展开更多
A recent study published in Nature Communications showed that essential modulatory roles of interfacial adhesion and mechanical microenvironments such as geometric constraints and extracellular matrix stiffness,in mic...A recent study published in Nature Communications showed that essential modulatory roles of interfacial adhesion and mechanical microenvironments such as geometric constraints and extracellular matrix stiffness,in microbehost cell interactions.This study utilized single-cell force spectroscopy and RNA sequencing to gain insight into the intrinsic mechanisms by which the mechanical microenvironment regulates bacterial-host interactions and therefore reveal potential interventions against bacterial invasion.Meanwhile,the adhesion forces involved in the bacterial–host interactions were recognized as a new indicator for assessing the extent of bacterial infection.Taken together,these findings demonstrate that interfacial adhesion forces and mechanical microenvironments play a dominant role in modulating functions and behaviors of microorganisms and host cells,which also provide a mechanobiology-inspired idea for the development of subsequent drug-resistant antimicrobials and broadspectrum antiviral drugs.展开更多
Many diseases and health conditions are closely related to various microbes,which participate in complex interactions with diverse drugs;nonetheless,the detailed targets of such drugs remain to be elucidated.Many exis...Many diseases and health conditions are closely related to various microbes,which participate in complex interactions with diverse drugs;nonetheless,the detailed targets of such drugs remain to be elucidated.Many existing studies have reported causal associations among drugs,gut microbes,or diseases,calling for a workflow to reveal their intricate interactions.In this study,we developed a systematic workflow comprising three modules to construct a Quorum Sensing-based Drug-Microbe-Disease(QSDMD)database(http://www.qsdmd.lbci.net/),which includes diverse interactions for more than 8,000 drugs,163 microbes,and 42 common diseases.Potential interactions between microbes and more than 8,000 drugs have been systematically studied by targeting microbial QS receptors combined with a docking-based virtual screening technique and in vitro experimental validations.Furthermore,we have constructed a QS-based drug-receptor interaction network,proposed a systematic framework including various drug-receptor-microbe-disease connections,and mapped a paradigmatic circular interaction network based on the QS-DMD,which can provide the underlying QS-based mechanisms for the reported causal associations.The QS-DMD will promote an understanding of personalized medicine and the development of potential therapies for diverse diseases.This work contributes to a paradigm for the construction of a molecule-receptor-microbe-disease interaction network for human health that may form one of the key knowledge maps of precision medicine in the future.展开更多
文摘In natural environments,plants are exposed to diverse microbiota that they interact with in complex ways.While plant-pathogen interactions have been intensely studied to understand defense mechanisms in plants,many microbes and microbial communities can have substantial beneficial effects on their plant host.Such beneficial effects include improved acquisition of nutrients,accelerated growth,resilience against pathogens,and improved resistance against abiotic stress conditions such as heat,drought,and salinity.However,the beneficial effects of bacterial strains or consortia on their host are often cultivar and species specific,posing an obstacle to their general application.Remarkably,many of the signals that trigger plant immune responses are molecularly highly similar and often identical in pathogenic and beneficial microbes.Thus,it is unclear what determines the outcome of a particular microbe-host interaction and which factors enable plants to distinguish beneficials from pathogens.To unravel the complex network of genetic,microbial,and metabolic interactions,including the signaling events mediating microbe-host interactions,comprehensive quantitative systems biology approaches will be needed.
基金supported by National Natural Science Foundation of China(Grant no.12372175).
文摘A recent study published in Nature Communications showed that essential modulatory roles of interfacial adhesion and mechanical microenvironments such as geometric constraints and extracellular matrix stiffness,in microbehost cell interactions.This study utilized single-cell force spectroscopy and RNA sequencing to gain insight into the intrinsic mechanisms by which the mechanical microenvironment regulates bacterial-host interactions and therefore reveal potential interventions against bacterial invasion.Meanwhile,the adhesion forces involved in the bacterial–host interactions were recognized as a new indicator for assessing the extent of bacterial infection.Taken together,these findings demonstrate that interfacial adhesion forces and mechanical microenvironments play a dominant role in modulating functions and behaviors of microorganisms and host cells,which also provide a mechanobiology-inspired idea for the development of subsequent drug-resistant antimicrobials and broadspectrum antiviral drugs.
基金supported by the National Key Research and Development Project of China(2019YFA0905600,2020YFA0907900)the National Natural Science Foundation of China(31570089,31770076,62172296)+1 种基金the Funds for Creative Research Groups of China(21621004)the New Century Outstanding Talent Support Program,Education Ministry of China。
文摘Many diseases and health conditions are closely related to various microbes,which participate in complex interactions with diverse drugs;nonetheless,the detailed targets of such drugs remain to be elucidated.Many existing studies have reported causal associations among drugs,gut microbes,or diseases,calling for a workflow to reveal their intricate interactions.In this study,we developed a systematic workflow comprising three modules to construct a Quorum Sensing-based Drug-Microbe-Disease(QSDMD)database(http://www.qsdmd.lbci.net/),which includes diverse interactions for more than 8,000 drugs,163 microbes,and 42 common diseases.Potential interactions between microbes and more than 8,000 drugs have been systematically studied by targeting microbial QS receptors combined with a docking-based virtual screening technique and in vitro experimental validations.Furthermore,we have constructed a QS-based drug-receptor interaction network,proposed a systematic framework including various drug-receptor-microbe-disease connections,and mapped a paradigmatic circular interaction network based on the QS-DMD,which can provide the underlying QS-based mechanisms for the reported causal associations.The QS-DMD will promote an understanding of personalized medicine and the development of potential therapies for diverse diseases.This work contributes to a paradigm for the construction of a molecule-receptor-microbe-disease interaction network for human health that may form one of the key knowledge maps of precision medicine in the future.
