We describe a technique for micro-patterning and immobilization of dyes on polymer substrates using a low-power visible laser for dye-excitation. Deposits from an aqueous medium containing the dye can be attached at a...We describe a technique for micro-patterning and immobilization of dyes on polymer substrates using a low-power visible laser for dye-excitation. Deposits from an aqueous medium containing the dye can be attached at any desired spot on the substrate simply by exposing the area to laser light. The area of the laser beam can control the spot-size of immobilized dye, in the range of 10 - 100 microns. The immobilization technique is characterized by micro-printing numerals, alphabets and patterns on polybutadiene substrates with Rhodamine (Rh6G) dye. Adsorption of laser-excited dye molecules within the polymer appears to be the mechanism for laser-printing technique.展开更多
目的探索单一微挤压一次性3D打印皮肤组织方法的可行性。方法从人皮肤真皮层分离、培养成纤维细胞并通过免疫荧光染色鉴定。以成纤维细胞和HaCaT细胞为种子细胞,用明胶、透明质酸和纤维蛋白原组成支架材料,通过微挤压法打印真皮层和表皮...目的探索单一微挤压一次性3D打印皮肤组织方法的可行性。方法从人皮肤真皮层分离、培养成纤维细胞并通过免疫荧光染色鉴定。以成纤维细胞和HaCaT细胞为种子细胞,用明胶、透明质酸和纤维蛋白原组成支架材料,通过微挤压法打印真皮层和表皮层,凝血酶交联支架材料。通过HE染色和免疫荧光染色观察3D打印皮肤组织的细胞分布。用活/死细胞染色试剂盒检测3D打印皮肤组织内细胞的活性。采用甲苯胺蓝渗透试验检测打印皮肤组织的屏障功能。结果分离培养的成纤维细胞波形蛋白阳性细胞占98%以上。当支架材料中纤维蛋白原浓度在真皮层和表皮层分别为5 mg/ml和1 mg/ml,成纤维细胞(4 x 106个/ml)打印8层,HaCaT细胞(5 x 106个/ml)打印1层时,制备的3D打印皮肤组织气液培养7 d后,HE染色和免疫荧光检测显示表皮层和真皮层分层良好,表皮层厚度为(63.5±3.5)|im。3D打印皮肤组织浸没培养2 d和气液培养7d,细胞活性分别为(90.2±0.9)%和(95.3±0.8)%°3D打印组织皮肤气液培养7 d后和正常皮肤具有类似的屏障功能。结论我们建立了单一微挤压一次性3D打印皮肤组织的方法,成功制备了结构、表皮层厚度和屏障功能类似于正常人皮肤组织的组织工程皮肤,有可能成为未来批量生产组织工程皮肤的有效方法。展开更多
文摘We describe a technique for micro-patterning and immobilization of dyes on polymer substrates using a low-power visible laser for dye-excitation. Deposits from an aqueous medium containing the dye can be attached at any desired spot on the substrate simply by exposing the area to laser light. The area of the laser beam can control the spot-size of immobilized dye, in the range of 10 - 100 microns. The immobilization technique is characterized by micro-printing numerals, alphabets and patterns on polybutadiene substrates with Rhodamine (Rh6G) dye. Adsorption of laser-excited dye molecules within the polymer appears to be the mechanism for laser-printing technique.
文摘目的探索单一微挤压一次性3D打印皮肤组织方法的可行性。方法从人皮肤真皮层分离、培养成纤维细胞并通过免疫荧光染色鉴定。以成纤维细胞和HaCaT细胞为种子细胞,用明胶、透明质酸和纤维蛋白原组成支架材料,通过微挤压法打印真皮层和表皮层,凝血酶交联支架材料。通过HE染色和免疫荧光染色观察3D打印皮肤组织的细胞分布。用活/死细胞染色试剂盒检测3D打印皮肤组织内细胞的活性。采用甲苯胺蓝渗透试验检测打印皮肤组织的屏障功能。结果分离培养的成纤维细胞波形蛋白阳性细胞占98%以上。当支架材料中纤维蛋白原浓度在真皮层和表皮层分别为5 mg/ml和1 mg/ml,成纤维细胞(4 x 106个/ml)打印8层,HaCaT细胞(5 x 106个/ml)打印1层时,制备的3D打印皮肤组织气液培养7 d后,HE染色和免疫荧光检测显示表皮层和真皮层分层良好,表皮层厚度为(63.5±3.5)|im。3D打印皮肤组织浸没培养2 d和气液培养7d,细胞活性分别为(90.2±0.9)%和(95.3±0.8)%°3D打印组织皮肤气液培养7 d后和正常皮肤具有类似的屏障功能。结论我们建立了单一微挤压一次性3D打印皮肤组织的方法,成功制备了结构、表皮层厚度和屏障功能类似于正常人皮肤组织的组织工程皮肤,有可能成为未来批量生产组织工程皮肤的有效方法。
