MicroRNAs(miRNAs)are known to fine-tune growth,development,and stress-induced responses.Osa-miR1873 is a rice-specific miRNA targeting LOC_Os05g01790.Here,we show that Osa-miR1873 fine-tunes rice immunity against Magn...MicroRNAs(miRNAs)are known to fine-tune growth,development,and stress-induced responses.Osa-miR1873 is a rice-specific miRNA targeting LOC_Os05g01790.Here,we show that Osa-miR1873 fine-tunes rice immunity against Magnaporthe oryzae and yield traits via LOC_Os05g01790.Osa-miR1873 was significantly upregulated in a susceptible accession but downregulated in a resistance accession at 24 h post-inoculation(hpi)of M.oryzae.Overexpressing Osa-miR1873 enhanced susceptibility to M.oryzae and compromised induction of defense responses.In contrast,blocking Osa-miR1873 through target mimicry compromised susceptibility to M.oryzae and enhanced induction of defense responses.Altered expression of Osa-miR1873 also resulted in some defects in yield traits,including grain numbers and seed setting rate.Moreover,overexpression of the target gene LOC_Os05g01790 increased rice blast disease resistance but severely penalized growth and yield.Taken together,we demonstrate that Osa-miR1873 fine-tunes the rice immunity-growth trade-off via LOC_Os05g01790,and blocking Osa-miR1873 could improve blast disease resistance without significant yield penalty.Thus,the Osa-miR1873-LOC_Os05g01790 regulatory module is valuable in balancing yield traits and blast resistance.展开更多
基金This work was supported by the National Natural Science Foundation of China(grant 31430072 and 31672090 to W.-M.W.).
文摘MicroRNAs(miRNAs)are known to fine-tune growth,development,and stress-induced responses.Osa-miR1873 is a rice-specific miRNA targeting LOC_Os05g01790.Here,we show that Osa-miR1873 fine-tunes rice immunity against Magnaporthe oryzae and yield traits via LOC_Os05g01790.Osa-miR1873 was significantly upregulated in a susceptible accession but downregulated in a resistance accession at 24 h post-inoculation(hpi)of M.oryzae.Overexpressing Osa-miR1873 enhanced susceptibility to M.oryzae and compromised induction of defense responses.In contrast,blocking Osa-miR1873 through target mimicry compromised susceptibility to M.oryzae and enhanced induction of defense responses.Altered expression of Osa-miR1873 also resulted in some defects in yield traits,including grain numbers and seed setting rate.Moreover,overexpression of the target gene LOC_Os05g01790 increased rice blast disease resistance but severely penalized growth and yield.Taken together,we demonstrate that Osa-miR1873 fine-tunes the rice immunity-growth trade-off via LOC_Os05g01790,and blocking Osa-miR1873 could improve blast disease resistance without significant yield penalty.Thus,the Osa-miR1873-LOC_Os05g01790 regulatory module is valuable in balancing yield traits and blast resistance.
