目的:探讨多发性骨髓瘤(multiple myeloma,MM)患者血清miR-424及miR-520的表达及其临床意义。方法:选取2015年1月至2019年12月我院收治的87例MM患者,依据国际分期系统分为Ⅰ期(n=18)、Ⅱ期(n=26)和Ⅲ期(n=43),根据免疫分型结果分为IgG型...目的:探讨多发性骨髓瘤(multiple myeloma,MM)患者血清miR-424及miR-520的表达及其临床意义。方法:选取2015年1月至2019年12月我院收治的87例MM患者,依据国际分期系统分为Ⅰ期(n=18)、Ⅱ期(n=26)和Ⅲ期(n=43),根据免疫分型结果分为IgG型(n=49)、IgA型(n=20)、轻链型(n=11)和非分泌型(n=7)。另选取同期60例正常者作为对照组。检测各组血清miR-424、miR-520、胱抑素C(Cys-C)及β2-微球蛋白(β2-MG)水平。应用受试者工作特征(ROC)曲线分析血清miR-424、miR-520、Cys-C及β2-MG水平诊断MM的价值。采用Pearson相关分析MM患者血清miR-424、miR-520水平与Cys-C及β2-MG的相关性。结果:MM组血清miR-424(2.93±1.35 vs 0.82±0.27)、miR-520(2.14±0.86 vs 0.56±0.18)、Cys-C(2.16±0.84 vs 0.75±0.18,mg/L)及β2-MG(6.24±2.37 vs 1.80±0.66,mg/L)水平均明显高于对照组(P<0.001)。MM患者Ⅲ期血清miR-424(5.20±2.46 vs 2.28±0.95,1.17±0.38)、miR-520(3.60±1.51 vs 1.87±0.72,0.83±0.26)、Cys-C(3.75±1.28 vs 1.64±0.53,0.90±0.21,mg/L)及β2-MG(8.82±3.14 vs 5.25±1.38,3.12±0.96,mg/L)水平均明显高于Ⅰ期和Ⅱ期(P<0.001),且Ⅱ期血清miR-424(2.28±0.95 vs 1.17±0.38)、miR-520(1.87±0.72 vs 0.83±0.26)、Cys-C(1.64±0.53 vs 0.90±0.21)及β2-MG(5.25±1.38 vs 3.12±0.96)水平均明显高于Ⅰ期(P<0.001)。MM患者IgG型血清miR-424(4.37±2.18 vs 2.40±1.06,2.27±0.93,2.35±0.84)及miR-520(3.22±1.37 vs 1.72±0.74,1.68±0.63,1.78±0.65)水平均明显高于IgA型、轻链型和非分泌型(P<0.001)。ROC曲线分析显示,miR-424、miR-520、Cys-C及β2-MG四项联合诊断MM的曲线下面积(0.963,95%CI:0.910~0.998)最大,其敏感度和特异度为97.0%和92.0%。相关分析结果显示,MM患者血清miR-424及miR-520表达水平与Cys-C及β2-MG均呈正相关(r=0.748,P<0.001,r=0.862,P<0.001,r=0.705,P<0.001,r=0.803,P<0.001)。结论:MM患者血清miR-424及miR-520表达水平明显升高,且随着MM病情分期进展呈升高趋势,联合Cys-C及β2-MG检测对MM诊断具有较高的�展开更多
Although the etiology of sciatica remains uncertain,there is increasing evidence that the disease process of sciatica is associated with the levels of inflammatory factors.Piperine,an alkaloid isolated from Piper nigr...Although the etiology of sciatica remains uncertain,there is increasing evidence that the disease process of sciatica is associated with the levels of inflammatory factors.Piperine,an alkaloid isolated from Piper nigrum,has previously been demonstrated to inhibit inflammation and analgesic effects.The purpose of this study is to verify the regulatory relationship between miR-520a and p65 and to explore how miR-520a/P65 affects the level of cytokines under the action of piperine,so as to play a therapeutic role in sciatica.Through ELISA experiment,we confirmed that four inflammatory factors(IL-1β,TNF-α,IL-10,TGF-β1)can be used as evaluation indexes of sciatica.The differentially expressed miRNA was screened as miR-520a,by microarray technology,and the downstream target of miR-520a was P65 by bioinformatics.Real-time fluorescence quantitative PCR confirmed that the expression of miR-520a was negatively correlated with pro-inflammatory cytokines,positively correlated with anti-inflammatory cytokines and negatively correlated with p65 expression at mRNA level.The expression of p65 was positively correlated with pro-inflammatory cytokines and negatively correlated with anti-inflammatory cytokines at the protein level verified by ELISA and Western blot.HE staining analysis showed that the nerve fibers were repaired by piprine,the vacuoles were significantly reduced,and the degree of nerve fiber damage was also improved.Immunohistochemical analysis showed that the expression of p65 decreased after administration of piperine.Dual-luciferase reporter gene assay confirmed that the luciferase signal decreased significantly after cotransfection of miR-520a mimics and p653'UTR recombinant plasmid.To sum up,in the rat model of non-compressed lumbar disc herniation,piperine plays a significant role in analgesia.MiR-520a can specifically and directly target P65,and piperine can promote the expression of miR-520a,then inhibit the expression of p65,down-regulate the pro-inflammatory factors IL-1βand TNF-α,and up-regulate the 展开更多
Objective:To investigate the effects of co-transfection of miR-520c-3p and miR-132 on proliferation and apoptosis of hepatocellular carcinoma Huh7.Methods:Hepatocellular carcinoma Huh7 was cultured in vitro and lipido...Objective:To investigate the effects of co-transfection of miR-520c-3p and miR-132 on proliferation and apoptosis of hepatocellular carcinoma Huh7.Methods:Hepatocellular carcinoma Huh7 was cultured in vitro and lipidosome was used to transfect miR-520c-3p and miR-132 respectively or together.The effects of transfection of miR-520c-3p and miR-132 on proliferation and apoptosis of Huh7 were detected by CCK8 and Annexin V staining and flow cytometry,and the expression level of the targeted gene of over-expressed miR-520c-3p and miR-132 was determined by Western blot and realtime PCR.Results:Compared with the control group,the proliferation ability of Huh7 of the single transfected and co-transfected miR-520c-3p and miR-132 decreased significantly,and the apoptosis ratio increased distinctly(P<0.05).Besides,the affect of the co-transfection group was better than that of the single transfection group.The protein levels of GPC3(Glypican-3) and YAP(Yes-associated protein),the target genes transfected only by miR-520c-3p and miR-132,respectively,reduced obviously(P<0.05),which was similar with the co-infected cells,but cells transfected by miR-132 only showed a decrease of YAP.Conclusions:The co-transfection of miR-520c-3p and miR-132 can target-regulate the expression of GPC3 and YAP,enhance the exhibition effect on proliferation of hepatocellular carcinoma Huh7 and induce cell apoptosis synergistically.展开更多
文摘目的:探讨多发性骨髓瘤(multiple myeloma,MM)患者血清miR-424及miR-520的表达及其临床意义。方法:选取2015年1月至2019年12月我院收治的87例MM患者,依据国际分期系统分为Ⅰ期(n=18)、Ⅱ期(n=26)和Ⅲ期(n=43),根据免疫分型结果分为IgG型(n=49)、IgA型(n=20)、轻链型(n=11)和非分泌型(n=7)。另选取同期60例正常者作为对照组。检测各组血清miR-424、miR-520、胱抑素C(Cys-C)及β2-微球蛋白(β2-MG)水平。应用受试者工作特征(ROC)曲线分析血清miR-424、miR-520、Cys-C及β2-MG水平诊断MM的价值。采用Pearson相关分析MM患者血清miR-424、miR-520水平与Cys-C及β2-MG的相关性。结果:MM组血清miR-424(2.93±1.35 vs 0.82±0.27)、miR-520(2.14±0.86 vs 0.56±0.18)、Cys-C(2.16±0.84 vs 0.75±0.18,mg/L)及β2-MG(6.24±2.37 vs 1.80±0.66,mg/L)水平均明显高于对照组(P<0.001)。MM患者Ⅲ期血清miR-424(5.20±2.46 vs 2.28±0.95,1.17±0.38)、miR-520(3.60±1.51 vs 1.87±0.72,0.83±0.26)、Cys-C(3.75±1.28 vs 1.64±0.53,0.90±0.21,mg/L)及β2-MG(8.82±3.14 vs 5.25±1.38,3.12±0.96,mg/L)水平均明显高于Ⅰ期和Ⅱ期(P<0.001),且Ⅱ期血清miR-424(2.28±0.95 vs 1.17±0.38)、miR-520(1.87±0.72 vs 0.83±0.26)、Cys-C(1.64±0.53 vs 0.90±0.21)及β2-MG(5.25±1.38 vs 3.12±0.96)水平均明显高于Ⅰ期(P<0.001)。MM患者IgG型血清miR-424(4.37±2.18 vs 2.40±1.06,2.27±0.93,2.35±0.84)及miR-520(3.22±1.37 vs 1.72±0.74,1.68±0.63,1.78±0.65)水平均明显高于IgA型、轻链型和非分泌型(P<0.001)。ROC曲线分析显示,miR-424、miR-520、Cys-C及β2-MG四项联合诊断MM的曲线下面积(0.963,95%CI:0.910~0.998)最大,其敏感度和特异度为97.0%和92.0%。相关分析结果显示,MM患者血清miR-424及miR-520表达水平与Cys-C及β2-MG均呈正相关(r=0.748,P<0.001,r=0.862,P<0.001,r=0.705,P<0.001,r=0.803,P<0.001)。结论:MM患者血清miR-424及miR-520表达水平明显升高,且随着MM病情分期进展呈升高趋势,联合Cys-C及β2-MG检测对MM诊断具有较高的�
基金supported by the National Natural Science Foundation of China(Nos.81760748,81550047)the Natural Science Foundation of Inner Mongolia Autonomous Region(Nos.2018LH08032,2017MS0831)Inner Mongolia Autonomous Region"Prairie Excellence"Project,West Light Foundation of the Chinese Academy of Sciences,the Major Science Foundation of Affiliated Hospital of Inner Mongolia Medical University(No.NY-FY ZD010).
文摘Although the etiology of sciatica remains uncertain,there is increasing evidence that the disease process of sciatica is associated with the levels of inflammatory factors.Piperine,an alkaloid isolated from Piper nigrum,has previously been demonstrated to inhibit inflammation and analgesic effects.The purpose of this study is to verify the regulatory relationship between miR-520a and p65 and to explore how miR-520a/P65 affects the level of cytokines under the action of piperine,so as to play a therapeutic role in sciatica.Through ELISA experiment,we confirmed that four inflammatory factors(IL-1β,TNF-α,IL-10,TGF-β1)can be used as evaluation indexes of sciatica.The differentially expressed miRNA was screened as miR-520a,by microarray technology,and the downstream target of miR-520a was P65 by bioinformatics.Real-time fluorescence quantitative PCR confirmed that the expression of miR-520a was negatively correlated with pro-inflammatory cytokines,positively correlated with anti-inflammatory cytokines and negatively correlated with p65 expression at mRNA level.The expression of p65 was positively correlated with pro-inflammatory cytokines and negatively correlated with anti-inflammatory cytokines at the protein level verified by ELISA and Western blot.HE staining analysis showed that the nerve fibers were repaired by piprine,the vacuoles were significantly reduced,and the degree of nerve fiber damage was also improved.Immunohistochemical analysis showed that the expression of p65 decreased after administration of piperine.Dual-luciferase reporter gene assay confirmed that the luciferase signal decreased significantly after cotransfection of miR-520a mimics and p653'UTR recombinant plasmid.To sum up,in the rat model of non-compressed lumbar disc herniation,piperine plays a significant role in analgesia.MiR-520a can specifically and directly target P65,and piperine can promote the expression of miR-520a,then inhibit the expression of p65,down-regulate the pro-inflammatory factors IL-1βand TNF-α,and up-regulate the
基金supported by Supported by Education Department of Hubei Province science and technology research project(NO.B2015230)Applied Fundamental Research Project of Wuhan Municipal Science and Technology Bureau(Grant No.2015061701011630)+1 种基金Medical Scientific Research Project of Health and Familly Planning Commission of Wuhan Municipality(Grant No.WX16E12)the fourth batch of "Hanyang Talent Associate Program"
文摘Objective:To investigate the effects of co-transfection of miR-520c-3p and miR-132 on proliferation and apoptosis of hepatocellular carcinoma Huh7.Methods:Hepatocellular carcinoma Huh7 was cultured in vitro and lipidosome was used to transfect miR-520c-3p and miR-132 respectively or together.The effects of transfection of miR-520c-3p and miR-132 on proliferation and apoptosis of Huh7 were detected by CCK8 and Annexin V staining and flow cytometry,and the expression level of the targeted gene of over-expressed miR-520c-3p and miR-132 was determined by Western blot and realtime PCR.Results:Compared with the control group,the proliferation ability of Huh7 of the single transfected and co-transfected miR-520c-3p and miR-132 decreased significantly,and the apoptosis ratio increased distinctly(P<0.05).Besides,the affect of the co-transfection group was better than that of the single transfection group.The protein levels of GPC3(Glypican-3) and YAP(Yes-associated protein),the target genes transfected only by miR-520c-3p and miR-132,respectively,reduced obviously(P<0.05),which was similar with the co-infected cells,but cells transfected by miR-132 only showed a decrease of YAP.Conclusions:The co-transfection of miR-520c-3p and miR-132 can target-regulate the expression of GPC3 and YAP,enhance the exhibition effect on proliferation of hepatocellular carcinoma Huh7 and induce cell apoptosis synergistically.
