Background:The pathogenesis of osteosarcoma(OS)is still unclear,and it is still necessary to find new targets and drugs for anti-OS.This study aimed to investigate the role and mechanism of the anti-OS effects of miR-...Background:The pathogenesis of osteosarcoma(OS)is still unclear,and it is still necessary to find new targets and drugs for anti-OS.This study aimed to investigate the role and mechanism of the anti-OS effects of miR-296-5p.Methods:We measured the expression of miR-296-5p in human OS cell lines and tissues.The effect of miR-296-5p and its target gene staphylococcal nuclease and tudor domain containing 1 on proliferation,migration,and invasion of human OS lines was examined.The Student's t test was used for statistical analysis.Results:We found that microRNA(miR)-296-5p was significantly downregulated in OS cell lines and tissues(control vs.OS,1.802±0.313 i/s.0.618±0.235,f=6.402,P<0.01).Overexpression of miR-296-5p suppressed proliferation,migration,and invasion of OA cells.SND1 was identified as a target of miR-296-5p by bioinformatic analysis and dual-luciferase reporter assay.Overexpression oiSNDl abrogated the effects induced by miR-296-5p upregulation(miRNA-296-5p vs.miRNA-296-5p+SND1,0.294±0.159 t/s.2.300±0.277,t=12.68,P=0.003).Conclusion:Our study indicates that miR-296-5p may function as a tumor suppressor by targeting SND1 in OS.展开更多
Objective To investigate the miR-296＇s function in prostate carcinoma（PCa） cells. Methods In order to profile the miRNA expression in LNCaP cells, the cultured cells were stimulated with androgen after 48-h starvat...Objective To investigate the miR-296＇s function in prostate carcinoma（PCa） cells. Methods In order to profile the miRNA expression in LNCaP cells, the cultured cells were stimulated with androgen after 48-h starvation, miRNA microarray analysis and Q-RT-PCR assay were performed. To characterize the effects of miR296 on PCa cells, CL-1 and PC-3 cells were transfected with miR-296 and antisense-miR-296, cell growth and apoptosis were then analyzed. Results The miR-296-5p expression was up-regulated by 2.22 folds in the CL-1 cells, which do not express significantly androgen receptor, than in LNCaP cells. Knockdown of miR-296-5p induced apoptosis of CL-1 cells, but not LNCaP cells. However, knockdown of miR-296-5p inhibited the growth rate of LNCaP cells cultured in absence of androgen. Conclusion MiR-296-5p could be important for development of prostate cancer from androgen dependence to androgen independence.展开更多
基金This study was granted by the National Natural Science Foundation of China(No.81572179 and No.81874018)the Clinical Special Program of Jiangsu Province(No.BL2014044)+4 种基金the Mingsheng Science and Technology Project of Suzhou City(No.SS201634 and No.SS201814)the Clinical Medical Center Project of Suzhou City(No.SZZX201504)Advantage Discipline Groups of the Second Affiliated Hospital of Soochow University(No.XKQ2015001)the Zhejiang Provincial Natural Science Foundation of China(Nos.LY15H060006 and LY16H060007)the Zhejiang Provincial Health Bureau Science Foundation of China(No.2018KY017).
文摘Background:The pathogenesis of osteosarcoma(OS)is still unclear,and it is still necessary to find new targets and drugs for anti-OS.This study aimed to investigate the role and mechanism of the anti-OS effects of miR-296-5p.Methods:We measured the expression of miR-296-5p in human OS cell lines and tissues.The effect of miR-296-5p and its target gene staphylococcal nuclease and tudor domain containing 1 on proliferation,migration,and invasion of human OS lines was examined.The Student's t test was used for statistical analysis.Results:We found that microRNA(miR)-296-5p was significantly downregulated in OS cell lines and tissues(control vs.OS,1.802±0.313 i/s.0.618±0.235,f=6.402,P<0.01).Overexpression of miR-296-5p suppressed proliferation,migration,and invasion of OA cells.SND1 was identified as a target of miR-296-5p by bioinformatic analysis and dual-luciferase reporter assay.Overexpression oiSNDl abrogated the effects induced by miR-296-5p upregulation(miRNA-296-5p vs.miRNA-296-5p+SND1,0.294±0.159 t/s.2.300±0.277,t=12.68,P=0.003).Conclusion:Our study indicates that miR-296-5p may function as a tumor suppressor by targeting SND1 in OS.
文摘Objective To investigate the miR-296＇s function in prostate carcinoma（PCa） cells. Methods In order to profile the miRNA expression in LNCaP cells, the cultured cells were stimulated with androgen after 48-h starvation, miRNA microarray analysis and Q-RT-PCR assay were performed. To characterize the effects of miR296 on PCa cells, CL-1 and PC-3 cells were transfected with miR-296 and antisense-miR-296, cell growth and apoptosis were then analyzed. Results The miR-296-5p expression was up-regulated by 2.22 folds in the CL-1 cells, which do not express significantly androgen receptor, than in LNCaP cells. Knockdown of miR-296-5p induced apoptosis of CL-1 cells, but not LNCaP cells. However, knockdown of miR-296-5p inhibited the growth rate of LNCaP cells cultured in absence of androgen. Conclusion MiR-296-5p could be important for development of prostate cancer from androgen dependence to androgen independence.
